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The map label for this gene is rsmB [H]

Identifier: 91781434

GI number: 91781434

Start: 65920

End: 67293

Strand: Direct

Name: rsmB [H]

Synonym: Bxe_A4412

Alternate gene names: 91781434

Gene position: 65920-67293 (Clockwise)

Preceding gene: 91781433

Following gene: 91781435

Centisome position: 1.35

GC content: 67.83

Gene sequence:

>1374_bases
ATGCTGCATCTCGCGCCCGAGTCGCTCGGCTTCGCGCTCGACTGCGCGGGCCAGGCTGTCGGCGCAGTGCGTCTCGGCGC
AGCGTTGCCGGCCGCGCTGCAATCCGTCTTCGTGTCCGCGCCCGAGGGTAGCGTCGCGGCCGCGCGCGGCGCGGTGCAGG
ACATCGCGTACCGCACCATGCGGCGTCTGGCGACCGCCGAGTGGCTGATCGCGAAGCTGGTGAAAAAGGCGCCGCCGCCG
CACGTCGGTCATGTGCTCGCCTGTTCGCTGGCCTTGCTTGTCGACGACGAAGCGAACGCAGCCTACGCGCCGTTCACGGT
GGTCGATCAGGCGGTGAGCGCGATCGCCGCGCGTCGTGAATTCGCTTTTGCCAAGGGGCTCGTCAATGCCGTGCTGCGCA
CTTTCCTGCGTGAGCGCGAGACGCTGTTGAGCGCCGCGCAAGCCGACGAAGTGGCGCGCTGGAACTACCCCGCCTGGTGG
ATCGATGCGGTGAAGCGCGCATGGCCCGACGCATGGCAGTCGGTGCTCGCCGCCGGCAACACGCAAGGTCCGCTGACGCT
GCGCGTGAACGCGCGCCGTTCGACTGTGGACGCCTACCTGCAACTGCTGCAGAACCAGCACATTGCCGCAAGCAAGGCGG
GAGACTACGCCGTCAAGCTCGCCACGCCGATGCCCGTTGACCGCATTCCGGGCTTCACCGACGGTGTCGTTTCGGTGCAG
GACGCCGGCGCGCAACTCGCCGCGCAACTGCTCGGCGTGCACGACGGTATGCGCGTGCTCGACGCCTGCGCCGCGCCCGG
CGGCAAGACCGGCCATCTGCTCGAACTGGCCAATCTTCAGCTCGTCGCGCTCGAAAGCGACGCCTCGCGTGCGCGCCGGA
TCGGCGAGAACCTGCAGCGCCTCAAGCTCGAAGCGGAAGTGCGGATCGGCGACGCCGGCACGCCCGCGAAATGGCACGAC
GACATCGACCAGCCATTCGACCGCATTCTCGCCGACGTGCCGTGTTCGGCGTCGGGCATCGTGCGGCGCCATCCGGACAT
TCGCTGGCTGCGCCGCGCGTCCGACATTCCCGCGCTGGTCGCCGAGCAGCGCCGCATTCTCGACGCGCTGTGGCCGCTCG
TGAAACCAGGCGGCGAGTTGCTCTACGTTACGTGTTCGATCTTTCCCGAAGAAGGCGAGTTGCAGGCGCAGTGGTTTGGA
AACAAGTATCAGGATGCGGTACGATTGGACGCGCCGGGGCAGCTGTTGCCTTCAGTCGCTCGCGCGCCCGCCGACGCATC
GGCCGGTTCCCATGCCGGACAATCCGCTGAAGACAGCGCCGGCGCGAACTCAGACCACGACGGATTCTTCTACGCGCGCT
TCCAGAAACGGTGA

Upstream 100 bases:

>100_bases
CGCGCGCTCCGCGCGGCCCGCCTTAGCCTTCTTCATGACCATAAAGCCTTCTTCGCGCACTGCTTCCGCACCGGCGCGTC
CGCGCGAATCCCGTTTGTCG

Downstream 100 bases:

>100_bases
CCATTAAACGCTTCTTCCCGCTTCGGCTCGCTGCCGTGCTCTGGATCGCGCTGGCCGTATGGCTGGCGGCGCCGGGCGTG
GCGCATGCTGACTCGATCGC

Product: Fmu, rRNA SAM-dependent methyltransferase

Products: NA

Alternate protein names: 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MLHLAPESLGFALDCAGQAVGAVRLGAALPAALQSVFVSAPEGSVAAARGAVQDIAYRTMRRLATAEWLIAKLVKKAPPP
HVGHVLACSLALLVDDEANAAYAPFTVVDQAVSAIAARREFAFAKGLVNAVLRTFLRERETLLSAAQADEVARWNYPAWW
IDAVKRAWPDAWQSVLAAGNTQGPLTLRVNARRSTVDAYLQLLQNQHIAASKAGDYAVKLATPMPVDRIPGFTDGVVSVQ
DAGAQLAAQLLGVHDGMRVLDACAAPGGKTGHLLELANLQLVALESDASRARRIGENLQRLKLEAEVRIGDAGTPAKWHD
DIDQPFDRILADVPCSASGIVRRHPDIRWLRRASDIPALVAEQRRILDALWPLVKPGGELLYVTCSIFPEEGELQAQWFG
NKYQDAVRLDAPGQLLPSVARAPADASAGSHAGQSAEDSAGANSDHDGFFYARFQKR

Sequences:

>Translated_457_residues
MLHLAPESLGFALDCAGQAVGAVRLGAALPAALQSVFVSAPEGSVAAARGAVQDIAYRTMRRLATAEWLIAKLVKKAPPP
HVGHVLACSLALLVDDEANAAYAPFTVVDQAVSAIAARREFAFAKGLVNAVLRTFLRERETLLSAAQADEVARWNYPAWW
IDAVKRAWPDAWQSVLAAGNTQGPLTLRVNARRSTVDAYLQLLQNQHIAASKAGDYAVKLATPMPVDRIPGFTDGVVSVQ
DAGAQLAAQLLGVHDGMRVLDACAAPGGKTGHLLELANLQLVALESDASRARRIGENLQRLKLEAEVRIGDAGTPAKWHD
DIDQPFDRILADVPCSASGIVRRHPDIRWLRRASDIPALVAEQRRILDALWPLVKPGGELLYVTCSIFPEEGELQAQWFG
NKYQDAVRLDAPGQLLPSVARAPADASAGSHAGQSAEDSAGANSDHDGFFYARFQKR
>Mature_457_residues
MLHLAPESLGFALDCAGQAVGAVRLGAALPAALQSVFVSAPEGSVAAARGAVQDIAYRTMRRLATAEWLIAKLVKKAPPP
HVGHVLACSLALLVDDEANAAYAPFTVVDQAVSAIAARREFAFAKGLVNAVLRTFLRERETLLSAAQADEVARWNYPAWW
IDAVKRAWPDAWQSVLAAGNTQGPLTLRVNARRSTVDAYLQLLQNQHIAASKAGDYAVKLATPMPVDRIPGFTDGVVSVQ
DAGAQLAAQLLGVHDGMRVLDACAAPGGKTGHLLELANLQLVALESDASRARRIGENLQRLKLEAEVRIGDAGTPAKWHD
DIDQPFDRILADVPCSASGIVRRHPDIRWLRRASDIPALVAEQRRILDALWPLVKPGGELLYVTCSIFPEEGELQAQWFG
NKYQDAVRLDAPGQLLPSVARAPADASAGSHAGQSAEDSAGANSDHDGFFYARFQKR

Specific function: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=273, Percent_Identity=27.8388278388278, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI76150623, Length=273, Percent_Identity=27.8388278388278, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI32698918, Length=195, Percent_Identity=31.2820512820513, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI39995082, Length=302, Percent_Identity=25.1655629139073, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI301336155, Length=207, Percent_Identity=28.9855072463768, Blast_Score=69, Evalue=7e-12,
Organism=Escherichia coli, GI2367212, Length=415, Percent_Identity=39.0361445783133, Blast_Score=276, Evalue=2e-75,
Organism=Escherichia coli, GI87081985, Length=322, Percent_Identity=27.9503105590062, Blast_Score=92, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17536757, Length=269, Percent_Identity=27.8810408921933, Blast_Score=98, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6319447, Length=333, Percent_Identity=26.1261261261261, Blast_Score=85, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6324268, Length=295, Percent_Identity=27.1186440677966, Blast_Score=81, Evalue=4e-16,
Organism=Drosophila melanogaster, GI22024126, Length=283, Percent_Identity=26.5017667844523, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24668781, Length=246, Percent_Identity=33.3333333333333, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21356513, Length=241, Percent_Identity=33.195020746888, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI19920494, Length=170, Percent_Identity=32.9411764705882, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: =2.1.1.176 [H]

Molecular weight: Translated: 49033; Mature: 49033

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: PS01153 NOL1_NOP2_SUN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHLAPESLGFALDCAGQAVGAVRLGAALPAALQSVFVSAPEGSVAAARGAVQDIAYRTM
CCCCCCHHHCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
RRLATAEWLIAKLVKKAPPPHVGHVLACSLALLVDDEANAAYAPFTVVDQAVSAIAARRE
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHHHHH
FAFAKGLVNAVLRTFLRERETLLSAAQADEVARWNYPAWWIDAVKRAWPDAWQSVLAAGN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCHHHHHHHHHCCC
TQGPLTLRVNARRSTVDAYLQLLQNQHIAASKAGDYAVKLATPMPVDRIPGFTDGVVSVQ
CCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHCCCCCCCEEEHH
DAGAQLAAQLLGVHDGMRVLDACAAPGGKTGHLLELANLQLVALESDASRARRIGENLQR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCEEEEEECCCHHHHHHHHHHHHH
LKLEAEVRIGDAGTPAKWHDDIDQPFDRILADVPCSASGIVRRHPDIRWLRRASDIPALV
HEEEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHCCCCHHHHHHHCCCHHHH
AEQRRILDALWPLVKPGGELLYVTCSIFPEEGELQAQWFGNKYQDAVRLDAPGQLLPSVA
HHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCEEHHHCCCCCCCCEEECCCHHHHHHHH
RAPADASAGSHAGQSAEDSAGANSDHDGFFYARFQKR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECC
>Mature Secondary Structure
MLHLAPESLGFALDCAGQAVGAVRLGAALPAALQSVFVSAPEGSVAAARGAVQDIAYRTM
CCCCCCHHHCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
RRLATAEWLIAKLVKKAPPPHVGHVLACSLALLVDDEANAAYAPFTVVDQAVSAIAARRE
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHHHHH
FAFAKGLVNAVLRTFLRERETLLSAAQADEVARWNYPAWWIDAVKRAWPDAWQSVLAAGN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCHHHHHHHHHCCC
TQGPLTLRVNARRSTVDAYLQLLQNQHIAASKAGDYAVKLATPMPVDRIPGFTDGVVSVQ
CCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHCCCCCCCEEEHH
DAGAQLAAQLLGVHDGMRVLDACAAPGGKTGHLLELANLQLVALESDASRARRIGENLQR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCEEEEEECCCHHHHHHHHHHHHH
LKLEAEVRIGDAGTPAKWHDDIDQPFDRILADVPCSASGIVRRHPDIRWLRRASDIPALV
HEEEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHCCCCHHHHHHHCCCHHHH
AEQRRILDALWPLVKPGGELLYVTCSIFPEEGELQAQWFGNKYQDAVRLDAPGQLLPSVA
HHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCEEHHHCCCCCCCCEEECCCHHHHHHHH
RAPADASAGSHAGQSAEDSAGANSDHDGFFYARFQKR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA