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The map label for this gene is cca [H]

Identifier: 161621311

GI number: 161621311

Start: 4631676

End: 4632908

Strand: Reverse

Name: cca [H]

Synonym: Bxe_A0201

Alternate gene names: 161621311

Gene position: 4632908-4631676 (Counterclockwise)

Preceding gene: 91785577

Following gene: 91785573

Centisome position: 94.63

GC content: 65.69

Gene sequence:

>1233_bases
ATGAACATCTATGCAGTAGGCGGCGCGATCCGCGACGAGTTACTGGGCGTACCGGTGCAGGATCGTGATTACGTGGTTGT
CGGCGCCACGCCGGAGCAGATGGTGGCGCAAGGCTACCGTCCGGTGGGCAAGGATTTTCCGGTGTTCCTGCATCCGCAGA
CTCATGAGGAGTACGCGCTCGCCCGCACTGAGCGCAAGACGGCGGCCGGCTATCACGGTTTCCAGTTTTTCTATGCGCCG
GATGTCACGCTGGAAGAAGACCTCGCGCGCCGCGATCTGACCATCAACGCCATGGCGCGCGAAGTGCGTCCGGACGGCGA
GTTGACCGGCCCGGTGATCGACCCGTTCAACGGCCAGGGTGATTTGCAGGCGCGCCTGTTTCGCCACGTCAGTGACGCGT
TTCTCGAAGACCCCGTGCGGATTTTACGGATCGCCCGGTTTGCGGCCCGTTTTGTGGACTTCACCGTCGCGCCGGAGACA
CTGGCGCTCATGCGCAAGATGGTGGCCGACGGCGAAGTGGACGCGCTGGTGGCCGAACGCGTGTGGCAGGAAGTGTCGCG
TGGTCTGATGGAAAGGAAGCCGTCGCGCATGTTCGGCGTGCTGCGCGAGTGCGGTGCGCTGGCGCGGATTCTGCCGGAAA
TCGACGCGCTGTTCGGCGTGCCGCAGCGCGCCGACTATCACCCCGAAGTGGACACCGGCGTGCACGTGATGATGGTGGTC
GATCATGCCGCGCAGCAGGGTTATACATTGCCGGTCCGGTTTGCGGCGCTGACGCATGACCTCGGCAAGGCGACCACACC
CGAAGACGTGCTGCCGCGGCATATCGGCCACGAAGGGCGCAGCGTGGATCTGCTGAAGCCCCTATGCGAGCGGCTCCGGG
TGCCGAATGATTGCCGCGATCTCGCGCTGCTGGTTGCGCGCGAGCACGGCAACATTCATCGCGTGATGGAAATGGGTGCT
GCTGCGCTGGTGAGGCTGATCGAGCGCTGCGATGCGATCCGCAAACCTGCGCGCTTTGCTGAAGCCTTGCAGGCTTGTGA
AGCCGACGCGCGTGGGCGGCTGGGGTTCGAGATGCGGGACTATCCGCAGGCCGAGCGGTTGAGGGTGGCGCTCGTCGCGG
CGCGCGGCGTGGACGCCGGCGCCGTGGCCAAACGGCTCGCCGACGCGCCGGCCGGCATCAAGGACGCCGTGCATCAGGAG
CGGGTCCGGGCTGTCGAAGCAGTGCTCGGCTAA

Upstream 100 bases:

>100_bases
GAGCTTACGTGCAGCGCGTGACGGAACTGCCGGCGGTGCAAGCGTGGATCGACGACGCGCTGAGCGAAACGTATGCGATC
GCTTATCAAGACGTATGCCC

Downstream 100 bases:

>100_bases
GACCTTCAGGTTGCGGGAGCGGCGGCGAGCGTGGAGTCCACGCCATTGCCGCCGCCGCCAAAGGCAGCCGAACCTAGAGC
AGTCTGGCCACAAGCGACAA

Product: multifunctional tRNA nucleotidyl transferase/2'3'-cyclic phosphodiesterase/2'nucleotidase/phosphatase

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase [H]

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MNIYAVGGAIRDELLGVPVQDRDYVVVGATPEQMVAQGYRPVGKDFPVFLHPQTHEEYALARTERKTAAGYHGFQFFYAP
DVTLEEDLARRDLTINAMAREVRPDGELTGPVIDPFNGQGDLQARLFRHVSDAFLEDPVRILRIARFAARFVDFTVAPET
LALMRKMVADGEVDALVAERVWQEVSRGLMERKPSRMFGVLRECGALARILPEIDALFGVPQRADYHPEVDTGVHVMMVV
DHAAQQGYTLPVRFAALTHDLGKATTPEDVLPRHIGHEGRSVDLLKPLCERLRVPNDCRDLALLVAREHGNIHRVMEMGA
AALVRLIERCDAIRKPARFAEALQACEADARGRLGFEMRDYPQAERLRVALVAARGVDAGAVAKRLADAPAGIKDAVHQE
RVRAVEAVLG

Sequences:

>Translated_410_residues
MNIYAVGGAIRDELLGVPVQDRDYVVVGATPEQMVAQGYRPVGKDFPVFLHPQTHEEYALARTERKTAAGYHGFQFFYAP
DVTLEEDLARRDLTINAMAREVRPDGELTGPVIDPFNGQGDLQARLFRHVSDAFLEDPVRILRIARFAARFVDFTVAPET
LALMRKMVADGEVDALVAERVWQEVSRGLMERKPSRMFGVLRECGALARILPEIDALFGVPQRADYHPEVDTGVHVMMVV
DHAAQQGYTLPVRFAALTHDLGKATTPEDVLPRHIGHEGRSVDLLKPLCERLRVPNDCRDLALLVAREHGNIHRVMEMGA
AALVRLIERCDAIRKPARFAEALQACEADARGRLGFEMRDYPQAERLRVALVAARGVDAGAVAKRLADAPAGIKDAVHQE
RVRAVEAVLG
>Mature_410_residues
MNIYAVGGAIRDELLGVPVQDRDYVVVGATPEQMVAQGYRPVGKDFPVFLHPQTHEEYALARTERKTAAGYHGFQFFYAP
DVTLEEDLARRDLTINAMAREVRPDGELTGPVIDPFNGQGDLQARLFRHVSDAFLEDPVRILRIARFAARFVDFTVAPET
LALMRKMVADGEVDALVAERVWQEVSRGLMERKPSRMFGVLRECGALARILPEIDALFGVPQRADYHPEVDTGVHVMMVV
DHAAQQGYTLPVRFAALTHDLGKATTPEDVLPRHIGHEGRSVDLLKPLCERLRVPNDCRDLALLVAREHGNIHRVMEMGA
AALVRLIERCDAIRKPARFAEALQACEADARGRLGFEMRDYPQAERLRVALVAARGVDAGAVAKRLADAPAGIKDAVHQE
RVRAVEAVLG

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789436, Length=386, Percent_Identity=56.2176165803109, Blast_Score=428, Evalue=1e-121,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 45454; Mature: 45454

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIYAVGGAIRDELLGVPVQDRDYVVVGATPEQMVAQGYRPVGKDFPVFLHPQTHEEYAL
CEEEEECCHHHHHHHCCCCCCCCEEEECCCHHHHHHCCCCCCCCCCCEEECCCCCHHHHH
ARTERKTAAGYHGFQFFYAPDVTLEEDLARRDLTINAMAREVRPDGELTGPVIDPFNGQG
HHHHHHHHCCCCCEEEEECCCCCHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCC
DLQARLFRHVSDAFLEDPVRILRIARFAARFVDFTVAPETLALMRKMVADGEVDALVAER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHH
VWQEVSRGLMERKPSRMFGVLRECGALARILPEIDALFGVPQRADYHPEVDTGVHVMMVV
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEE
DHAAQQGYTLPVRFAALTHDLGKATTPEDVLPRHIGHEGRSVDLLKPLCERLRVPNDCRD
EHHHHCCCCCEEHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHH
LALLVAREHGNIHRVMEMGAAALVRLIERCDAIRKPARFAEALQACEADARGRLGFEMRD
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCHHC
YPQAERLRVALVAARGVDAGAVAKRLADAPAGIKDAVHQERVRAVEAVLG
CCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MNIYAVGGAIRDELLGVPVQDRDYVVVGATPEQMVAQGYRPVGKDFPVFLHPQTHEEYAL
CEEEEECCHHHHHHHCCCCCCCCEEEECCCHHHHHHCCCCCCCCCCCEEECCCCCHHHHH
ARTERKTAAGYHGFQFFYAPDVTLEEDLARRDLTINAMAREVRPDGELTGPVIDPFNGQG
HHHHHHHHCCCCCEEEEECCCCCHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCC
DLQARLFRHVSDAFLEDPVRILRIARFAARFVDFTVAPETLALMRKMVADGEVDALVAER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHH
VWQEVSRGLMERKPSRMFGVLRECGALARILPEIDALFGVPQRADYHPEVDTGVHVMMVV
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEE
DHAAQQGYTLPVRFAALTHDLGKATTPEDVLPRHIGHEGRSVDLLKPLCERLRVPNDCRD
EHHHHCCCCCEEHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHH
LALLVAREHGNIHRVMEMGAAALVRLIERCDAIRKPARFAEALQACEADARGRLGFEMRD
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCHHC
YPQAERLRVALVAARGVDAGAVAKRLADAPAGIKDAVHQERVRAVEAVLG
CCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA