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The map label for this gene is purB [H]

Identifier: 91790491

GI number: 91790491

Start: 4952145

End: 4953524

Strand: Direct

Name: purB [H]

Synonym: Bpro_4662

Alternate gene names: 91790491

Gene position: 4952145-4953524 (Clockwise)

Preceding gene: 91790489

Following gene: 91790494

Centisome position: 95.23

GC content: 61.67

Gene sequence:

>1380_bases
ATGAGCTTTTCTACCATCAACGCCTTGTCTCCGCTGGACGGCCGCTACGCCGGCAAACTCAGCCAGTTACGTCCCCTGAT
GTCCGAGCAAGGCTATATGCACCGCCGGGTCCAGGTGGAAGTGGCCTGGTTCATCGCCCTTTCGGACGCGAAGTTTGCCG
AATTCAAGCCGCTCAGCCCCGGGGCGCGCACTTACCTGCTGGGCCTCGTCAAGAATTTCTCGGAAAACGATGCGGTGGCC
ATCAAGGAGATTGAAAAAACCACCAACCACGACGTCAAGGCCGTGGAGTACTGGATCAAGTCCAAATTCGAAGCGCGGCC
GGAATTGCAGGCGGCCGGTGAGTTTGTGCATTTCGCCTGCACCAGCGAAGACATCAACAACACCAGCCATGCGCTGCAGC
TCAAAAGCAGCCGCGAGCTGGTACTGCTGCCGGCCCTGGACAAGGTGATTGCGCAGATGCGCAAGATGGCGCACACCTAC
GCGGCGGAGCCCATGCTCAGCCGCACCCACGGCCAGACCGCCAGTCCGACCACGGTAGGCAAGGAAATCGCCAACGTGGT
GGTGCGCCTGCAGGCGGCGCGCGAGAAAATCGCCGGCATCAGGCTGATGGCCAAAATGAACGGCGCCGTAGGCAACTACA
ACGCCCATCTGTCGGCCTGGCCCGACTTTGACTGGGAAGCCTTCAGCCGCAACGTGATTGAAACGCCCGAGCCACTGGGC
CTGGGCCTGACCTTCCAGCCCTACAGCATCCAGATCGAGCCGCATGACTACATGGCCGAGCTGTTTGACGCCGTCGCCCG
CACCAACACCATCCTGATCGACTTTGCGCGGGACGTCTGGGGTTATGTGAGCGTGGGCTATTTCAAGCAGCGCCTCAAGG
AGGGCGAAATCGGCTCCTCCACCATGCCGCACAAGGTGAACCCTATCGACTTTGAAAACGCCGAAGGCAACCTGGGCCTG
GCCAATGCGCTGCTCAGGCACCTGAGCGAAAAGCTGCCGGTGTCCCGCTGGCAGCGCGACCTGACCGACTCGACCGTGCT
GCGCAACATGGGCGTGGCGCTGGGTTACGCCGTGCTGGCCTACAACTCACTGGGCGTGGGGCTGGGCAAGCTGGAACTCA
ACCGGGAAGCGCTGCAGGAGGACCTGGACAGCTCGTGGGAGGTGCTGGCCGAACCGATACAGACCGTCATGCGCCGCTAC
GGCGTGCAGGGCGCCTACGAAAAGCTCAAGGAAGTAACGCGCGGTAAAACCGTCAAGGCTGAAGACCTGCATGCCCTGAT
CCGGTCCCTGCAAATTCCTGATGCAGAAAAACAGCGCCTGCTGGCCATGACACCGGCCAGCTACGTCGGCCTGGCTGCCA
CCCTGGCCAGACGGGTTTAA

Upstream 100 bases:

>100_bases
CGACACGGCCCGAAGGCGAGCAAATCTGCCAGGTTTTGGGCACCCCCGCTGCCACCGCGCGACCGGGCCATTCCCCGGGC
GACCAGCCTACAATTGCGGC

Downstream 100 bases:

>100_bases
CGCCCCTTGTCGTTGTTGTCGCTGCTGCAGCGGCAGCGCTAAACCGGCATGGCTGCACCGCAATGCCAGCACTGCTCGAA
CCCGCCTTCGACGGTTTCCC

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MSFSTINALSPLDGRYAGKLSQLRPLMSEQGYMHRRVQVEVAWFIALSDAKFAEFKPLSPGARTYLLGLVKNFSENDAVA
IKEIEKTTNHDVKAVEYWIKSKFEARPELQAAGEFVHFACTSEDINNTSHALQLKSSRELVLLPALDKVIAQMRKMAHTY
AAEPMLSRTHGQTASPTTVGKEIANVVVRLQAAREKIAGIRLMAKMNGAVGNYNAHLSAWPDFDWEAFSRNVIETPEPLG
LGLTFQPYSIQIEPHDYMAELFDAVARTNTILIDFARDVWGYVSVGYFKQRLKEGEIGSSTMPHKVNPIDFENAEGNLGL
ANALLRHLSEKLPVSRWQRDLTDSTVLRNMGVALGYAVLAYNSLGVGLGKLELNREALQEDLDSSWEVLAEPIQTVMRRY
GVQGAYEKLKEVTRGKTVKAEDLHALIRSLQIPDAEKQRLLAMTPASYVGLAATLARRV

Sequences:

>Translated_459_residues
MSFSTINALSPLDGRYAGKLSQLRPLMSEQGYMHRRVQVEVAWFIALSDAKFAEFKPLSPGARTYLLGLVKNFSENDAVA
IKEIEKTTNHDVKAVEYWIKSKFEARPELQAAGEFVHFACTSEDINNTSHALQLKSSRELVLLPALDKVIAQMRKMAHTY
AAEPMLSRTHGQTASPTTVGKEIANVVVRLQAAREKIAGIRLMAKMNGAVGNYNAHLSAWPDFDWEAFSRNVIETPEPLG
LGLTFQPYSIQIEPHDYMAELFDAVARTNTILIDFARDVWGYVSVGYFKQRLKEGEIGSSTMPHKVNPIDFENAEGNLGL
ANALLRHLSEKLPVSRWQRDLTDSTVLRNMGVALGYAVLAYNSLGVGLGKLELNREALQEDLDSSWEVLAEPIQTVMRRY
GVQGAYEKLKEVTRGKTVKAEDLHALIRSLQIPDAEKQRLLAMTPASYVGLAATLARRV
>Mature_458_residues
SFSTINALSPLDGRYAGKLSQLRPLMSEQGYMHRRVQVEVAWFIALSDAKFAEFKPLSPGARTYLLGLVKNFSENDAVAI
KEIEKTTNHDVKAVEYWIKSKFEARPELQAAGEFVHFACTSEDINNTSHALQLKSSRELVLLPALDKVIAQMRKMAHTYA
AEPMLSRTHGQTASPTTVGKEIANVVVRLQAAREKIAGIRLMAKMNGAVGNYNAHLSAWPDFDWEAFSRNVIETPEPLGL
GLTFQPYSIQIEPHDYMAELFDAVARTNTILIDFARDVWGYVSVGYFKQRLKEGEIGSSTMPHKVNPIDFENAEGNLGLA
NALLRHLSEKLPVSRWQRDLTDSTVLRNMGVALGYAVLAYNSLGVGLGKLELNREALQEDLDSSWEVLAEPIQTVMRRYG
VQGAYEKLKEVTRGKTVKAEDLHALIRSLQIPDAEKQRLLAMTPASYVGLAATLARRV

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787376, Length=456, Percent_Identity=57.6754385964912, Blast_Score=549, Evalue=1e-157,
Organism=Drosophila melanogaster, GI24647570, Length=335, Percent_Identity=27.1641791044776, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013539
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF08328 ASL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 51132; Mature: 51001

Theoretical pI: Translated: 7.86; Mature: 7.86

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFSTINALSPLDGRYAGKLSQLRPLMSEQGYMHRRVQVEVAWFIALSDAKFAEFKPLSP
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEEEECCCCCCCCCCCCC
GARTYLLGLVKNFSENDAVAIKEIEKTTNHDVKAVEYWIKSKFEARPELQAAGEFVHFAC
CHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHCCCEEEEEE
TSEDINNTSHALQLKSSRELVLLPALDKVIAQMRKMAHTYAAEPMLSRTHGQTASPTTVG
CCCCCCCCHHEEEECCCCCEEEECHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCHHHH
KEIANVVVRLQAAREKIAGIRLMAKMNGAVGNYNAHLSAWPDFDWEAFSRNVIETPEPLG
HHHHHHHHHHHHHHHHHHHHHHEEHHCCCCCCCCCCEECCCCCCHHHHCCCCCCCCCCCC
LGLTFQPYSIQIEPHDYMAELFDAVARTNTILIDFARDVWGYVSVGYFKQRLKEGEIGSS
CCEEECCCEEEECCHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCC
TMPHKVNPIDFENAEGNLGLANALLRHLSEKLPVSRWQRDLTDSTVLRNMGVALGYAVLA
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
YNSLGVGLGKLELNREALQEDLDSSWEVLAEPIQTVMRRYGVQGAYEKLKEVTRGKTVKA
HHHCCCCCCHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCH
EDLHALIRSLQIPDAEKQRLLAMTPASYVGLAATLARRV
HHHHHHHHHHCCCCHHHCCEEEECCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SFSTINALSPLDGRYAGKLSQLRPLMSEQGYMHRRVQVEVAWFIALSDAKFAEFKPLSP
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEEEECCCCCCCCCCCCC
GARTYLLGLVKNFSENDAVAIKEIEKTTNHDVKAVEYWIKSKFEARPELQAAGEFVHFAC
CHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHCCCEEEEEE
TSEDINNTSHALQLKSSRELVLLPALDKVIAQMRKMAHTYAAEPMLSRTHGQTASPTTVG
CCCCCCCCHHEEEECCCCCEEEECHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCHHHH
KEIANVVVRLQAAREKIAGIRLMAKMNGAVGNYNAHLSAWPDFDWEAFSRNVIETPEPLG
HHHHHHHHHHHHHHHHHHHHHHEEHHCCCCCCCCCCEECCCCCCHHHHCCCCCCCCCCCC
LGLTFQPYSIQIEPHDYMAELFDAVARTNTILIDFARDVWGYVSVGYFKQRLKEGEIGSS
CCEEECCCEEEECCHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCC
TMPHKVNPIDFENAEGNLGLANALLRHLSEKLPVSRWQRDLTDSTVLRNMGVALGYAVLA
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
YNSLGVGLGKLELNREALQEDLDSSWEVLAEPIQTVMRRYGVQGAYEKLKEVTRGKTVKA
HHHCCCCCCHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCH
EDLHALIRSLQIPDAEKQRLLAMTPASYVGLAATLARRV
HHHHHHHHHHCCCCHHHCCEEEECCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]