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The map label for this gene is dacB [H]

Identifier: 91790451

GI number: 91790451

Start: 4903108

End: 4904625

Strand: Direct

Name: dacB [H]

Synonym: Bpro_4622

Alternate gene names: 91790451

Gene position: 4903108-4904625 (Clockwise)

Preceding gene: 91790447

Following gene: 91790452

Centisome position: 94.29

GC content: 69.76

Gene sequence:

>1518_bases
ATGCTTTTCCACTCACCCCGCGCCCGCTTGAAGGCGCACCTGGCCGCCGGCCTGCTGGCCGGGCTGATCAGTCTGACAAG
CATCCCGGCCGCCGCCCAGACAGCGGCCCAGGCCCTGCCCCCCGAAGTGGCGGCCCTGCTGGCGCAAGCCAGGGTGCCGC
GCGACGCACTGGCCGCCGTGGTGGTGGACGCCGCACCGGCCCTGAATGGCCAGTCCGCCCCCCTGCTGAGCTGGCGCGCC
AGCGCCGCCATGAACCCGGCCTCGGTCATGAAGCTGGTGACCACTTACGCCGCCCTGGAGCTGCTGGGCCCGTCATTCAC
CTGGAACACACCGGTGTATGTCGACGGCACCGTCGCGGATGGTGTGCTGCAGGGCAACCTCATCATTCAGGGCAGGGGCG
ACCCCAAGCTGGTGCTGGAGCGGCTGTGGCTGCTGCTGCGCCGGGTGCAGGGCCTGGGCATCAAGAGCATCACCGGCGAC
ATCCTGCTGGACCGCAGCGCGTTTGCCCTCACCGCGCAAAACTCGGGCGACTTTGACGGCGAACCGCTGCGGCCCTACAA
CGCGGCGCCCGATGCGCTGCTGATCAACTACAAATCGGTGGTGATGACCTTTGCGCCCCATCCCGGCAGCGGCATCGCCA
GCGTGAGCTTTGAGCCGCCCCTGGCCAATGTGCAGATGCAGGCCAGCGTGCCGCTGTCGGCGGGCCAGGGTGCAGGCGCT
TCGCTGCTGGAGTGCGGCGACTACCGGGGACGGCTGAAAGCCGACTTCAGCGACCCGTTTCGCATCACCTTCAGCGGTAG
CTACCCGGCAGGCTGCGGCGAAAAGGTCTGGCCCGTGGCCTACATCGACCCGACGAGTTATGCGGAGCGGGCCGTGGCGG
GCCTGTGGCAGGACATGGGCGGGCGCCTGGGCGGCCGGGTCCGCGAGGGCCGCGCGCCCCTGGGCGTGAAGCCGGCCTTC
GAGGTGGTGTCGCCGACGCTGGCCGAGGTGATTCGCGACATCAACAAATACAGCAACAACGTGATGGCGCAGCAGCTGTT
CCTGACCCTGAGCCTGGTGCCGGGCCAGCCACGCCCGGGTGGCCTGGACGATGCGGCCAGCAATGAGGCCTCGCGCGAGG
TGGTGCGCAGCTGGTGGCGCGAGCGCCTGGGCGAACAGGACATGCCGGTGCTGGACAACGGCTCAGGCCTGTCGCGGCTG
GAGCGTGCCACTCCGCAGGGGCTGGCGCGGCTGCTGCAGACCGCCTATGTCTCGGGCGCCATGCCGGAGCTGATGGCCTC
GCTGCCGATCAGCGGCGTGGACGGCACGCTCCGGCGCAGCAAATCGCGCATGTCGCAGGGCTGGGCTCACCTGAAATCCG
GCTCCCTGCGCGACGCCAATGCGCTGGCCGGCTATGTGCACACGCCAAGCGGCCGGCGCCTGGTGGTGGTGGCCATCGTC
AACCATCCGAATGCGCCGGCAGCGCGGCCCGCCCTGGAAGCGCTGGTGAACTGGGCGCTGAAGGAGGGCAGCAAATGA

Upstream 100 bases:

>100_bases
GGTTGGCAGGCCGCCGTCGGCGAACACGCATCCGTTGATTATCCGGCCTGGCGGCCCACCCGGGGTTTTGCAGGGTCGGA
CCAACGCAGATAATCACCCC

Downstream 100 bases:

>100_bases
GCCTGCCGATCGACCTGACCGACCTGGTGGGCACGCTGGCCGCCTGCCTGACCACCCTGAGCTTTTTGCCGCAGGCCTGG
CTCACCTTCAAGACGCGCGA

Product: D-alanyl-D-alanine carboxypeptidase PBP3

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; D-alanyl-D-alanine endopeptidase; DD-endopeptidase; Penicillin-binding protein 4; PBP-4 [H]

Number of amino acids: Translated: 505; Mature: 505

Protein sequence:

>505_residues
MLFHSPRARLKAHLAAGLLAGLISLTSIPAAAQTAAQALPPEVAALLAQARVPRDALAAVVVDAAPALNGQSAPLLSWRA
SAAMNPASVMKLVTTYAALELLGPSFTWNTPVYVDGTVADGVLQGNLIIQGRGDPKLVLERLWLLLRRVQGLGIKSITGD
ILLDRSAFALTAQNSGDFDGEPLRPYNAAPDALLINYKSVVMTFAPHPGSGIASVSFEPPLANVQMQASVPLSAGQGAGA
SLLECGDYRGRLKADFSDPFRITFSGSYPAGCGEKVWPVAYIDPTSYAERAVAGLWQDMGGRLGGRVREGRAPLGVKPAF
EVVSPTLAEVIRDINKYSNNVMAQQLFLTLSLVPGQPRPGGLDDAASNEASREVVRSWWRERLGEQDMPVLDNGSGLSRL
ERATPQGLARLLQTAYVSGAMPELMASLPISGVDGTLRRSKSRMSQGWAHLKSGSLRDANALAGYVHTPSGRRLVVVAIV
NHPNAPAARPALEALVNWALKEGSK

Sequences:

>Translated_505_residues
MLFHSPRARLKAHLAAGLLAGLISLTSIPAAAQTAAQALPPEVAALLAQARVPRDALAAVVVDAAPALNGQSAPLLSWRA
SAAMNPASVMKLVTTYAALELLGPSFTWNTPVYVDGTVADGVLQGNLIIQGRGDPKLVLERLWLLLRRVQGLGIKSITGD
ILLDRSAFALTAQNSGDFDGEPLRPYNAAPDALLINYKSVVMTFAPHPGSGIASVSFEPPLANVQMQASVPLSAGQGAGA
SLLECGDYRGRLKADFSDPFRITFSGSYPAGCGEKVWPVAYIDPTSYAERAVAGLWQDMGGRLGGRVREGRAPLGVKPAF
EVVSPTLAEVIRDINKYSNNVMAQQLFLTLSLVPGQPRPGGLDDAASNEASREVVRSWWRERLGEQDMPVLDNGSGLSRL
ERATPQGLARLLQTAYVSGAMPELMASLPISGVDGTLRRSKSRMSQGWAHLKSGSLRDANALAGYVHTPSGRRLVVVAIV
NHPNAPAARPALEALVNWALKEGSK
>Mature_505_residues
MLFHSPRARLKAHLAAGLLAGLISLTSIPAAAQTAAQALPPEVAALLAQARVPRDALAAVVVDAAPALNGQSAPLLSWRA
SAAMNPASVMKLVTTYAALELLGPSFTWNTPVYVDGTVADGVLQGNLIIQGRGDPKLVLERLWLLLRRVQGLGIKSITGD
ILLDRSAFALTAQNSGDFDGEPLRPYNAAPDALLINYKSVVMTFAPHPGSGIASVSFEPPLANVQMQASVPLSAGQGAGA
SLLECGDYRGRLKADFSDPFRITFSGSYPAGCGEKVWPVAYIDPTSYAERAVAGLWQDMGGRLGGRVREGRAPLGVKPAF
EVVSPTLAEVIRDINKYSNNVMAQQLFLTLSLVPGQPRPGGLDDAASNEASREVVRSWWRERLGEQDMPVLDNGSGLSRL
ERATPQGLARLLQTAYVSGAMPELMASLPISGVDGTLRRSKSRMSQGWAHLKSGSLRDANALAGYVHTPSGRRLVVVAIV
NHPNAPAARPALEALVNWALKEGSK

Specific function: Not involved in transpeptidation but exclusively catalyzes a DD-carboxypeptidase and DD-endopeptidase reaction [H]

COG id: COG2027

COG function: function code M; D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 4)

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S13 family [H]

Homologues:

Organism=Escherichia coli, GI1789573, Length=439, Percent_Identity=28.246013667426, Blast_Score=108, Evalue=9e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR000667 [H]

Pfam domain/function: PF02113 Peptidase_S13 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 53446; Mature: 53446

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFHSPRARLKAHLAAGLLAGLISLTSIPAAAQTAAQALPPEVAALLAQARVPRDALAAV
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHH
VVDAAPALNGQSAPLLSWRASAAMNPASVMKLVTTYAALELLGPSFTWNTPVYVDGTVAD
HHHCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCC
GVLQGNLIIQGRGDPKLVLERLWLLLRRVQGLGIKSITGDILLDRSAFALTAQNSGDFDG
CEEECCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCEEEEEECCCCCCCC
EPLRPYNAAPDALLINYKSVVMTFAPHPGSGIASVSFEPPLANVQMQASVPLSAGQGAGA
CCCCCCCCCCCEEEEEEEHEEEEECCCCCCCEEEEECCCCCCCEEEEECCCCCCCCCCCC
SLLECGDYRGRLKADFSDPFRITFSGSYPAGCGEKVWPVAYIDPTSYAERAVAGLWQDMG
HHHHHCCCCCCEECCCCCCEEEEECCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHH
GRLGGRVREGRAPLGVKPAFEVVSPTLAEVIRDINKYSNNVMAQQLFLTLSLVPGQPRPG
HHHCCCEECCCCCCCCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCC
GLDDAASNEASREVVRSWWRERLGEQDMPVLDNGSGLSRLERATPQGLARLLQTAYVSGA
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHCCHHHHHHHHHHHHHCCC
MPELMASLPISGVDGTLRRSKSRMSQGWAHLKSGSLRDANALAGYVHTPSGRRLVVVAIV
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHEEEEECCCCCEEEEEEEE
NHPNAPAARPALEALVNWALKEGSK
ECCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLFHSPRARLKAHLAAGLLAGLISLTSIPAAAQTAAQALPPEVAALLAQARVPRDALAAV
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHH
VVDAAPALNGQSAPLLSWRASAAMNPASVMKLVTTYAALELLGPSFTWNTPVYVDGTVAD
HHHCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCC
GVLQGNLIIQGRGDPKLVLERLWLLLRRVQGLGIKSITGDILLDRSAFALTAQNSGDFDG
CEEECCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCEEEEEECCCCCCCC
EPLRPYNAAPDALLINYKSVVMTFAPHPGSGIASVSFEPPLANVQMQASVPLSAGQGAGA
CCCCCCCCCCCEEEEEEEHEEEEECCCCCCCEEEEECCCCCCCEEEEECCCCCCCCCCCC
SLLECGDYRGRLKADFSDPFRITFSGSYPAGCGEKVWPVAYIDPTSYAERAVAGLWQDMG
HHHHHCCCCCCEECCCCCCEEEEECCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHH
GRLGGRVREGRAPLGVKPAFEVVSPTLAEVIRDINKYSNNVMAQQLFLTLSLVPGQPRPG
HHHCCCEECCCCCCCCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCC
GLDDAASNEASREVVRSWWRERLGEQDMPVLDNGSGLSRLERATPQGLARLLQTAYVSGA
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHCCHHHHHHHHHHHHHCCC
MPELMASLPISGVDGTLRRSKSRMSQGWAHLKSGSLRDANALAGYVHTPSGRRLVVVAIV
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHEEEEECCCCCEEEEEEEE
NHPNAPAARPALEALVNWALKEGSK
ECCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2040429; 9278503; 2046551 [H]