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The map label for this gene is narK [H]

Identifier: 91790426

GI number: 91790426

Start: 4873917

End: 4875179

Strand: Reverse

Name: narK [H]

Synonym: Bpro_4597

Alternate gene names: 91790426

Gene position: 4875179-4873917 (Counterclockwise)

Preceding gene: 91790427

Following gene: 91790425

Centisome position: 93.75

GC content: 61.6

Gene sequence:

>1263_bases
ATGACCTCTGAACTGGGTAATTCAAGAAAAGCGTGGTCGGTGCTCATTGTCAGCACGCTGGCGTTCACCGTTTGCTTCAT
GGTCTGGATGATGTTCGGCGTCATCGGCATACCGATCAAGAAGATGCTCAACCTCAACGCCACGGAGTTCGGCCTGCTTG
CGGCCACGCCCGTGCTGACGGGCTCCCTGATCCGCGTTCCGCTGGGTATCTGGACCGACCGCTACGGCGGGCGCATGGTC
ATGGCGATCCTGATGGCTGTCACGGTCCCCGCGATCTGGCTCATGAGTTACGCCACCGCGTACTGGCATTTTCTGGTGAT
CGGCCTGTTTGTGGGCCTGGCCGGCGGTTCGTTCTCTGTGGGTACGCCCTATGTGGCGCGCTGGTTTCCGAAAAACCGCC
AGGGCATGGCGATGGGCGTCTATGGCGCCGGCAATTCGGGCGCTGCCGTCAACAAGTTTGTGGCGCCCGTGTTGCTGGTG
GCTTTCGGCTGGGCCATGGTGCCGCAGGTGTATGCCGTCATCATGCTGGGCGCGCTGGTGCTGTTCTGGCTGTTCAGCTA
CAGCGACCCTTCGCACCTGGTGCCCAGCCACGTCAAGTTCAGCGACCAGCTCAAGGCGCTGAAAGACCCCAAGGTGATCA
AGTACTGCCAGTACTACAGCATCGTGTTCGGCGGCTATGTGGCGCTGTCGCTCTGGATGGTTCAGTACTACGTCGGTGAA
TTTGGCCTGGATATCCGTGTGGCGGCCTTGCTGGCGGCGTGCTTCTCGCTGCCGGGCGGCGTGTTGCGCGCTTTCGGCGG
CGTGCTGTCCGACAGATACGGCGCGCACAGCGTCACCTGGTGGGTGATGTGGGTGAGCTGGGTCTGCCTGTTCCTGCTGT
CCTACCCGCAGACCGACTTCACCATCCTCACGGTCAACGGACCCGCAAGCTTTCACATCGGGCTGAACGTCTACACCTTC
ACCGCGCTGATGTTCACGCTGGGCATCGCCTGGGCCTTCGGCAAGGCCAGTGTTTTCAAGTACATCAGCGAAGACTACGG
CGCGAACATCGGCGGCATCAGCGGCATCGTGGGCCTGGCAGGCGGCCTGGGTGGCTTTGTGCTGCCCATCATCTTCGGTG
GGCTGATGGACCTGACCGGCATCCGCTCCAGCGCCTTCATGTTCATGTACGGCGTGGTCTGCGTTTCACTGATGTGGATG
TACTGGACCGAAGTCCGCGGTACTGAAGTCATGGGCAGGAACGCGGCTGGCGCTCAGGCCTGA

Upstream 100 bases:

>100_bases
GCCGCCATCGCCTTTTCTGCCTGATCTGCACGTTCCAAAGGAGGATGTTCAAGGGGGCGGGAAAGGTCGACAGTCATGCC
ATGTGTCTGGAGAAACATGC

Downstream 100 bases:

>100_bases
AACGGAACCCTTTTCGAGAAATCACCATGAATACAAAAACCATTTCCGGCACCGCTTCCACCAGTTCCACCGTTTCCGGT
GTTGACATCATCGACTGGCG

Product: major facilitator transporter

Products: Proton [Cytoplasm]; nitrite [Periplasm] [C]

Alternate protein names: Nitrite facilitator [H]

Number of amino acids: Translated: 420; Mature: 419

Protein sequence:

>420_residues
MTSELGNSRKAWSVLIVSTLAFTVCFMVWMMFGVIGIPIKKMLNLNATEFGLLAATPVLTGSLIRVPLGIWTDRYGGRMV
MAILMAVTVPAIWLMSYATAYWHFLVIGLFVGLAGGSFSVGTPYVARWFPKNRQGMAMGVYGAGNSGAAVNKFVAPVLLV
AFGWAMVPQVYAVIMLGALVLFWLFSYSDPSHLVPSHVKFSDQLKALKDPKVIKYCQYYSIVFGGYVALSLWMVQYYVGE
FGLDIRVAALLAACFSLPGGVLRAFGGVLSDRYGAHSVTWWVMWVSWVCLFLLSYPQTDFTILTVNGPASFHIGLNVYTF
TALMFTLGIAWAFGKASVFKYISEDYGANIGGISGIVGLAGGLGGFVLPIIFGGLMDLTGIRSSAFMFMYGVVCVSLMWM
YWTEVRGTEVMGRNAAGAQA

Sequences:

>Translated_420_residues
MTSELGNSRKAWSVLIVSTLAFTVCFMVWMMFGVIGIPIKKMLNLNATEFGLLAATPVLTGSLIRVPLGIWTDRYGGRMV
MAILMAVTVPAIWLMSYATAYWHFLVIGLFVGLAGGSFSVGTPYVARWFPKNRQGMAMGVYGAGNSGAAVNKFVAPVLLV
AFGWAMVPQVYAVIMLGALVLFWLFSYSDPSHLVPSHVKFSDQLKALKDPKVIKYCQYYSIVFGGYVALSLWMVQYYVGE
FGLDIRVAALLAACFSLPGGVLRAFGGVLSDRYGAHSVTWWVMWVSWVCLFLLSYPQTDFTILTVNGPASFHIGLNVYTF
TALMFTLGIAWAFGKASVFKYISEDYGANIGGISGIVGLAGGLGGFVLPIIFGGLMDLTGIRSSAFMFMYGVVCVSLMWM
YWTEVRGTEVMGRNAAGAQA
>Mature_419_residues
TSELGNSRKAWSVLIVSTLAFTVCFMVWMMFGVIGIPIKKMLNLNATEFGLLAATPVLTGSLIRVPLGIWTDRYGGRMVM
AILMAVTVPAIWLMSYATAYWHFLVIGLFVGLAGGSFSVGTPYVARWFPKNRQGMAMGVYGAGNSGAAVNKFVAPVLLVA
FGWAMVPQVYAVIMLGALVLFWLFSYSDPSHLVPSHVKFSDQLKALKDPKVIKYCQYYSIVFGGYVALSLWMVQYYVGEF
GLDIRVAALLAACFSLPGGVLRAFGGVLSDRYGAHSVTWWVMWVSWVCLFLLSYPQTDFTILTVNGPASFHIGLNVYTFT
ALMFTLGIAWAFGKASVFKYISEDYGANIGGISGIVGLAGGLGGFVLPIIFGGLMDLTGIRSSAFMFMYGVVCVSLMWMY
WTEVRGTEVMGRNAAGAQA

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787743, Length=443, Percent_Identity=24.3792325056433, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI1787475, Length=446, Percent_Identity=26.6816143497758, Blast_Score=86, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 45712; Mature: 45581

Theoretical pI: Translated: 9.39; Mature: 9.39

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSELGNSRKAWSVLIVSTLAFTVCFMVWMMFGVIGIPIKKMLNLNATEFGLLAATPVLT
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHH
GSLIRVPLGIWTDRYGGRMVMAILMAVTVPAIWLMSYATAYWHFLVIGLFVGLAGGSFSV
CHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GTPYVARWFPKNRQGMAMGVYGAGNSGAAVNKFVAPVLLVAFGWAMVPQVYAVIMLGALV
CCCHHHHCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFWLFSYSDPSHLVPSHVKFSDQLKALKDPKVIKYCQYYSIVFGGYVALSLWMVQYYVGE
HHHHHCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FGLDIRVAALLAACFSLPGGVLRAFGGVLSDRYGAHSVTWWVMWVSWVCLFLLSYPQTDF
CCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCE
TILTVNGPASFHIGLNVYTFTALMFTLGIAWAFGKASVFKYISEDYGANIGGISGIVGLA
EEEEECCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
GGLGGFVLPIIFGGLMDLTGIRSSAFMFMYGVVCVSLMWMYWTEVRGTEVMGRNAAGAQA
CCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCC
>Mature Secondary Structure 
TSELGNSRKAWSVLIVSTLAFTVCFMVWMMFGVIGIPIKKMLNLNATEFGLLAATPVLT
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHH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PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: nitrite [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: nitrite [Cytoplasm] + Proton [Periplasm] = Proton [Cytoplasm] + nitrite [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8846791; 9384377; 9353933 [H]