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The map label for this gene is narK [H]

Identifier: 91790425

GI number: 91790425

Start: 4872232

End: 4873890

Strand: Reverse

Name: narK [H]

Synonym: Bpro_4596

Alternate gene names: 91790425

Gene position: 4873890-4872232 (Counterclockwise)

Preceding gene: 91790426

Following gene: 91790424

Centisome position: 93.72

GC content: 61.97

Gene sequence:

>1659_bases
ATGAATACAAAAACCATTTCCGGCACCGCTTCCACCAGTTCCACCGTTTCCGGTGTTGACATCATCGACTGGCGTCCCGA
GGACGACCAGTTCTGGGAGAGCACGGGAAAACGCATCGCCTACCGCAACCTCTGGATCTCCGTGCCCAGCCTGCTGTGCG
GCTTTGCCGTCTGGGGCATGTGGGGCATCATCACGGTCCAGATGCTCAACCTGGGCTTTCCATTCACCCAGGCCGAGCTG
TTCACGCTGACGGCCATTGCGGGCCTGGCTGGCGCCACCATGCGCATTCCGGCGTCCTTCCTGATCCGGCTGGCCGGCGG
GCGCAACACCATTTTCCTGACGACCTCCATGCTGCTGGCGCCAGCGATAGGCACCGGCATCGTGCTCCAGCACCCCGAAT
GGCCCTTGTGGTCCTTCCAGCTGATGGCCCTGTGGTCGGGGGTGGGTGGCGGAAATTTCGCCAGCTCCATGTCCAACATC
AGCACCTTTTTCCCCAAGCGCTTGCAGGGCACGGCGCTGGGGCTCAATGCGGGACTGGGCAATTTCGGTGTCACGGCCAT
GCAGGTCGTGATCCCGCTGGTGATGACGCTGAGCGTCTTCGGCAGCTTCGGGGGAGATTCGATGACCCTGGTGAAGGACA
GCGGCTGGATTTTCGGAAAAATCGTCGCCGGCACGCCCACCTGGATCCAGAACGCCGGCTTCACCTGGCTGCTGTCGCTG
GTGCCGCTGTCGATTCTCTGCTTCTTCGGCATGAACAACCTCACGACGGTGTCGCCCGCGATTGGCGGCACGATCCCGGC
CTTTCTCAAGATCATCTGGCTGTACACGCTGTCGTTTGTGCCGGCCGGCATCGGCCTGTATCTCTACCTGCCCAAGCCCA
CCGGGCTGGGCCTGCTGAACATGTGGATTGCGATGCCCCTGATCGTGGCCAGCACCCTGATGGTGCTGAAGGTCACGGCC
TTTGGCCCGATGAAGGAAAACATTGCCAAGCAGTTCGCGATTTTCAGCAACAAGCACACCTGGTCGCTGACCCTGCTGTA
CATCGTGACTTTCGGCTCGTTCATCGGCTTTTCGATGGCCTTGCCGCTCGCCATCACGGTGATCTTCGGCATCAGCCACG
TGCCGGACGCGGCAGGCGTCATTCAGCACACATTGAAAAATCCGAACGCACCCTCGGCACTGACCTTCGCCTGGATAGGC
CCCTTTGTCGGCGCCTTGATCCGGCCCGTGGGAGGGTGGGTGTCGGACAAGGTCGGCGGCTCCATCGTGACCCAAATCAT
TTCCGCTGTCATGGTCGTCGCTTCGGCCGCGGTCGGCTACGTCATGCTGCTGGCCTACAAGTCGGCCGCGCCCGAGCAGT
ACTTCCTGCTCTTCATGGTGCTGTTCGTCGTGCTGTTTGCGGCCAGCGGCATCGGCAACGGCTCCACCTTCCGCACCATT
GGCGTCATCTTCGACCGCCAGCAGGCCGGCCCTGTGCTCGGCTGGACTTCGGCCATTGCCGCTTACGGCGCCTTCATCGC
CCCGGTGGTGATCGGTGCACAGATCAAGGCCGGCACGCCGGAAAACGCGATGTATGGCTTTGCCATCTTCTATGCCCTGT
GCCTGGTGCTCAACTGGTGGTTCTACCTGCGCCGCGGCGCCGAGATCAAGAACCCCTGA

Upstream 100 bases:

>100_bases
TGATGTGGATGTACTGGACCGAAGTCCGCGGTACTGAAGTCATGGGCAGGAACGCGGCTGGCGCTCAGGCCTGAAACGGA
ACCCTTTTCGAGAAATCACC

Downstream 100 bases:

>100_bases
CTCACCGCCCATCCGTTTTTTACCGCCTTACCGGAGAACCAAATGAGTCATTTTCTTGATCGACTGAATCACTTTTCAAA
TCCACGGGAATCCTTTTCTG

Product: nitrate/nitrite antiporter

Products: NA

Alternate protein names: Nitrite facilitator 1 [H]

Number of amino acids: Translated: 552; Mature: 552

Protein sequence:

>552_residues
MNTKTISGTASTSSTVSGVDIIDWRPEDDQFWESTGKRIAYRNLWISVPSLLCGFAVWGMWGIITVQMLNLGFPFTQAEL
FTLTAIAGLAGATMRIPASFLIRLAGGRNTIFLTTSMLLAPAIGTGIVLQHPEWPLWSFQLMALWSGVGGGNFASSMSNI
STFFPKRLQGTALGLNAGLGNFGVTAMQVVIPLVMTLSVFGSFGGDSMTLVKDSGWIFGKIVAGTPTWIQNAGFTWLLSL
VPLSILCFFGMNNLTTVSPAIGGTIPAFLKIIWLYTLSFVPAGIGLYLYLPKPTGLGLLNMWIAMPLIVASTLMVLKVTA
FGPMKENIAKQFAIFSNKHTWSLTLLYIVTFGSFIGFSMALPLAITVIFGISHVPDAAGVIQHTLKNPNAPSALTFAWIG
PFVGALIRPVGGWVSDKVGGSIVTQIISAVMVVASAAVGYVMLLAYKSAAPEQYFLLFMVLFVVLFAASGIGNGSTFRTI
GVIFDRQQAGPVLGWTSAIAAYGAFIAPVVIGAQIKAGTPENAMYGFAIFYALCLVLNWWFYLRRGAEIKNP

Sequences:

>Translated_552_residues
MNTKTISGTASTSSTVSGVDIIDWRPEDDQFWESTGKRIAYRNLWISVPSLLCGFAVWGMWGIITVQMLNLGFPFTQAEL
FTLTAIAGLAGATMRIPASFLIRLAGGRNTIFLTTSMLLAPAIGTGIVLQHPEWPLWSFQLMALWSGVGGGNFASSMSNI
STFFPKRLQGTALGLNAGLGNFGVTAMQVVIPLVMTLSVFGSFGGDSMTLVKDSGWIFGKIVAGTPTWIQNAGFTWLLSL
VPLSILCFFGMNNLTTVSPAIGGTIPAFLKIIWLYTLSFVPAGIGLYLYLPKPTGLGLLNMWIAMPLIVASTLMVLKVTA
FGPMKENIAKQFAIFSNKHTWSLTLLYIVTFGSFIGFSMALPLAITVIFGISHVPDAAGVIQHTLKNPNAPSALTFAWIG
PFVGALIRPVGGWVSDKVGGSIVTQIISAVMVVASAAVGYVMLLAYKSAAPEQYFLLFMVLFVVLFAASGIGNGSTFRTI
GVIFDRQQAGPVLGWTSAIAAYGAFIAPVVIGAQIKAGTPENAMYGFAIFYALCLVLNWWFYLRRGAEIKNP
>Mature_552_residues
MNTKTISGTASTSSTVSGVDIIDWRPEDDQFWESTGKRIAYRNLWISVPSLLCGFAVWGMWGIITVQMLNLGFPFTQAEL
FTLTAIAGLAGATMRIPASFLIRLAGGRNTIFLTTSMLLAPAIGTGIVLQHPEWPLWSFQLMALWSGVGGGNFASSMSNI
STFFPKRLQGTALGLNAGLGNFGVTAMQVVIPLVMTLSVFGSFGGDSMTLVKDSGWIFGKIVAGTPTWIQNAGFTWLLSL
VPLSILCFFGMNNLTTVSPAIGGTIPAFLKIIWLYTLSFVPAGIGLYLYLPKPTGLGLLNMWIAMPLIVASTLMVLKVTA
FGPMKENIAKQFAIFSNKHTWSLTLLYIVTFGSFIGFSMALPLAITVIFGISHVPDAAGVIQHTLKNPNAPSALTFAWIG
PFVGALIRPVGGWVSDKVGGSIVTQIISAVMVVASAAVGYVMLLAYKSAAPEQYFLLFMVLFVVLFAASGIGNGSTFRTI
GVIFDRQQAGPVLGWTSAIAAYGAFIAPVVIGAQIKAGTPENAMYGFAIFYALCLVLNWWFYLRRGAEIKNP

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787475, Length=272, Percent_Identity=41.1764705882353, Blast_Score=180, Evalue=2e-46,
Organism=Escherichia coli, GI1787743, Length=248, Percent_Identity=40.7258064516129, Blast_Score=166, Evalue=5e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004737 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 59137; Mature: 59137

Theoretical pI: Translated: 9.96; Mature: 9.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTKTISGTASTSSTVSGVDIIDWRPEDDQFWESTGKRIAYRNLWISVPSLLCGFAVWGM
CCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHH
WGIITVQMLNLGFPFTQAELFTLTAIAGLAGATMRIPASFLIRLAGGRNTIFLTTSMLLA
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHCCCHHHHHEECCCCCEEEEEHHHHHH
PAIGTGIVLQHPEWPLWSFQLMALWSGVGGGNFASSMSNISTFFPKRLQGTALGLNAGLG
HHCCCCEEEECCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCC
NFGVTAMQVVIPLVMTLSVFGSFGGDSMTLVKDSGWIFGKIVAGTPTWIQNAGFTWLLSL
CCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEEEECCCCHHHHCCCHHHHHHH
VPLSILCFFGMNNLTTVSPAIGGTIPAFLKIIWLYTLSFVPAGIGLYLYLPKPTGLGLLN
HHHHHHHHHCCCCCCEECCHHCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHH
MWIAMPLIVASTLMVLKVTAFGPMKENIAKQFAIFSNKHTWSLTLLYIVTFGSFIGFSMA
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
LPLAITVIFGISHVPDAAGVIQHTLKNPNAPSALTFAWIGPFVGALIRPVGGWVSDKVGG
HHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCH
SIVTQIISAVMVVASAAVGYVMLLAYKSAAPEQYFLLFMVLFVVLFAASGIGNGSTFRTI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEE
GVIFDRQQAGPVLGWTSAIAAYGAFIAPVVIGAQIKAGTPENAMYGFAIFYALCLVLNWW
EEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHHHH
FYLRRGAEIKNP
HHHHCCCCCCCC
>Mature Secondary Structure
MNTKTISGTASTSSTVSGVDIIDWRPEDDQFWESTGKRIAYRNLWISVPSLLCGFAVWGM
CCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHH
WGIITVQMLNLGFPFTQAELFTLTAIAGLAGATMRIPASFLIRLAGGRNTIFLTTSMLLA
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHCCCHHHHHEECCCCCEEEEEHHHHHH
PAIGTGIVLQHPEWPLWSFQLMALWSGVGGGNFASSMSNISTFFPKRLQGTALGLNAGLG
HHCCCCEEEECCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCC
NFGVTAMQVVIPLVMTLSVFGSFGGDSMTLVKDSGWIFGKIVAGTPTWIQNAGFTWLLSL
CCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEEEECCCCHHHHCCCHHHHHHH
VPLSILCFFGMNNLTTVSPAIGGTIPAFLKIIWLYTLSFVPAGIGLYLYLPKPTGLGLLN
HHHHHHHHHCCCCCCEECCHHCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHH
MWIAMPLIVASTLMVLKVTAFGPMKENIAKQFAIFSNKHTWSLTLLYIVTFGSFIGFSMA
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
LPLAITVIFGISHVPDAAGVIQHTLKNPNAPSALTFAWIGPFVGALIRPVGGWVSDKVGG
HHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCH
SIVTQIISAVMVVASAAVGYVMLLAYKSAAPEQYFLLFMVLFVVLFAASGIGNGSTFRTI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEE
GVIFDRQQAGPVLGWTSAIAAYGAFIAPVVIGAQIKAGTPENAMYGFAIFYALCLVLNWW
EEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHHHH
FYLRRGAEIKNP
HHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2668029; 8905232; 9278503; 2657652 [H]