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The map label for this gene is narH [H]

Identifier: 91790423

GI number: 91790423

Start: 4866770

End: 4868302

Strand: Reverse

Name: narH [H]

Synonym: Bpro_4594

Alternate gene names: 91790423

Gene position: 4868302-4866770 (Counterclockwise)

Preceding gene: 91790424

Following gene: 91790422

Centisome position: 93.62

GC content: 60.93

Gene sequence:

>1533_bases
ATGAAGGTACGCGCGCAAATCGGCATGGTGCTGAACCTGGACAAGTGCATTGGCTGCCACACCTGCTCAGTCACCTGCAA
AAACGTCTGGACCAGCCGGACCGGCATGGAGTACGCCTGGTTCAACAACGTTGAAACCAAGCCCGGCATCGGTTACCCGA
AGGAGTGGGAAAACCAGGACAAGTGGAACGGCGGCTGGATTCGCAAGAGCGACGGTTCGATTGAGCCGCGCCAGGGAGGC
AAGTGGAAGCTGCTGATGCGCATCTTTGCCAACCCGAACATGCCGCAGATCGACGACTACTACGAGCCCTTCACCTTCGA
CTACGACCATTTGCAGTCGGCGCCCGAGATGAAGGCCGCACCAACGGCGCGCCCGCGCAGCCTGATCACCGGCAAGCGCA
TGGAGAAAATCGAATGGGGCCCGAACTGGGAGGAAATCCTGGGCGGTGAATTCTCCAAGCGCAGCAAGGACGCCAATCTG
GCGAACTTCGACCAGGTCCAGAAAGACATGGTGGGTCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGA
GCATTGCCTGAACCCGGCCTGCGTGGCCTCCTGTCCTTCGGGCTCCATCTACAAGCGCGAGGAAGACGGCATCGTGCTGA
TCGACCAGGACAAGTGCCGCGGCTGGCGCATGTGTGTGTCGGGCTGCCCGTACAAAAAAATCTACTACAACTGGAAAAGC
GGCAAGGCCGAGAAGTGCACGTTTTGCTATCCGCGCATCGAAGCCGGGCAACCCACCGTGTGCAGCGAAACCTGCGTCGG
ACGCATCCGTTACCTCGGCGTGGTGCTGTATGACGCCGACCGCATCCAGGAAGCGGCCAGTGTGGAAGACGACAAGGACC
TGTACCAGGCCCAGCTCGACATCTTCCTGGACCCGAACGATCCGAAAGTGATTGAACAGGCGCGCAAGGACGGCATTCCA
GAAGCCTGGCTGGAAGGCGCCCGCCGCAGTCCGGTCTACAAGATGGCTGTGGAATGGAAGGTGGCGTTGCCGCTGCATCC
CGAGTACCGTACGCTGCCCATGGTCTGGTATGTGCCGCCGCTGTCGCCTATCACCTCGGCGGCCAATGCCGGACAGATCG
GCATCAATGGCGAACTGCCCGACATCGCGCAGATGCGCATTCCCGTGCAGTACCTGGCCAACCTGCTGACGGCAGGCGAC
ACTGCGCCGGTGATCCGTTCGCTGCAGCGCATGCTGGCCATGCGCTCCTACCAGCGCAGCAAGCATGTGGACCAGGTGCA
GAACCTGGCCGTGCTGGAGCAGGCCGGGCTCTCGGTGGCCGAGGTCGAGGAGATGTACCACATCATGGCGATTGCCAACT
ACGAAGACCGTTTCGTGATTCCGACCACCCATCGCGAATACGCCGAGAACGCGTTCGACATGCGCGGTGGCTGCGGCTTC
ACCTTCGGCAACGGCTGCTCCGACGGCGCCAGCGACGTCAGCCTGTTTGGCGGCAACCGCAAACGAACTATTCCCATCAA
GGTGGAGGTCTGA

Upstream 100 bases:

>100_bases
ATGAATTCGTCGTGGTGCGCAAGATGGACAAGGTGGACTGGCTGGATACGCCGCGCGATGACGAGCGTGCGTTCGCCGTG
CAACAACTGGGAGAAGCAGC

Downstream 100 bases:

>100_bases
ACCATGGCACTTTTTAACAACCCCACTATGGCTTCGAGAAGCCTGCGCGTGCTGGCCAGGCTGCTCGGTTATCCCGACGC
CGGGTTGCGCGGCGACCTGG

Product: respiratory nitrate reductase subunit beta

Products: NA

Alternate protein names: Nitrate reductase A subunit beta; Quinol-nitrate oxidoreductase subunit beta [H]

Number of amino acids: Translated: 510; Mature: 510

Protein sequence:

>510_residues
MKVRAQIGMVLNLDKCIGCHTCSVTCKNVWTSRTGMEYAWFNNVETKPGIGYPKEWENQDKWNGGWIRKSDGSIEPRQGG
KWKLLMRIFANPNMPQIDDYYEPFTFDYDHLQSAPEMKAAPTARPRSLITGKRMEKIEWGPNWEEILGGEFSKRSKDANL
ANFDQVQKDMVGQFENTFMMYLPRLCEHCLNPACVASCPSGSIYKREEDGIVLIDQDKCRGWRMCVSGCPYKKIYYNWKS
GKAEKCTFCYPRIEAGQPTVCSETCVGRIRYLGVVLYDADRIQEAASVEDDKDLYQAQLDIFLDPNDPKVIEQARKDGIP
EAWLEGARRSPVYKMAVEWKVALPLHPEYRTLPMVWYVPPLSPITSAANAGQIGINGELPDIAQMRIPVQYLANLLTAGD
TAPVIRSLQRMLAMRSYQRSKHVDQVQNLAVLEQAGLSVAEVEEMYHIMAIANYEDRFVIPTTHREYAENAFDMRGGCGF
TFGNGCSDGASDVSLFGGNRKRTIPIKVEV

Sequences:

>Translated_510_residues
MKVRAQIGMVLNLDKCIGCHTCSVTCKNVWTSRTGMEYAWFNNVETKPGIGYPKEWENQDKWNGGWIRKSDGSIEPRQGG
KWKLLMRIFANPNMPQIDDYYEPFTFDYDHLQSAPEMKAAPTARPRSLITGKRMEKIEWGPNWEEILGGEFSKRSKDANL
ANFDQVQKDMVGQFENTFMMYLPRLCEHCLNPACVASCPSGSIYKREEDGIVLIDQDKCRGWRMCVSGCPYKKIYYNWKS
GKAEKCTFCYPRIEAGQPTVCSETCVGRIRYLGVVLYDADRIQEAASVEDDKDLYQAQLDIFLDPNDPKVIEQARKDGIP
EAWLEGARRSPVYKMAVEWKVALPLHPEYRTLPMVWYVPPLSPITSAANAGQIGINGELPDIAQMRIPVQYLANLLTAGD
TAPVIRSLQRMLAMRSYQRSKHVDQVQNLAVLEQAGLSVAEVEEMYHIMAIANYEDRFVIPTTHREYAENAFDMRGGCGF
TFGNGCSDGASDVSLFGGNRKRTIPIKVEV
>Mature_510_residues
MKVRAQIGMVLNLDKCIGCHTCSVTCKNVWTSRTGMEYAWFNNVETKPGIGYPKEWENQDKWNGGWIRKSDGSIEPRQGG
KWKLLMRIFANPNMPQIDDYYEPFTFDYDHLQSAPEMKAAPTARPRSLITGKRMEKIEWGPNWEEILGGEFSKRSKDANL
ANFDQVQKDMVGQFENTFMMYLPRLCEHCLNPACVASCPSGSIYKREEDGIVLIDQDKCRGWRMCVSGCPYKKIYYNWKS
GKAEKCTFCYPRIEAGQPTVCSETCVGRIRYLGVVLYDADRIQEAASVEDDKDLYQAQLDIFLDPNDPKVIEQARKDGIP
EAWLEGARRSPVYKMAVEWKVALPLHPEYRTLPMVWYVPPLSPITSAANAGQIGINGELPDIAQMRIPVQYLANLLTAGD
TAPVIRSLQRMLAMRSYQRSKHVDQVQNLAVLEQAGLSVAEVEEMYHIMAIANYEDRFVIPTTHREYAENAFDMRGGCGF
TFGNGCSDGASDVSLFGGNRKRTIPIKVEV

Specific function: The nitrate reductase enzyme complex allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The beta chain is an electron transfer unit containing four cysteine clusters involved in the formation of iron-sulfur centers. Electrons ar

COG id: COG1140

COG function: function code C; Nitrate reductase beta subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787478, Length=507, Percent_Identity=71.7948717948718, Blast_Score=788, Evalue=0.0,
Organism=Escherichia coli, GI1787740, Length=507, Percent_Identity=71.4003944773175, Blast_Score=786, Evalue=0.0,
Organism=Escherichia coli, GI1787872, Length=140, Percent_Identity=35.7142857142857, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787122, Length=140, Percent_Identity=35.7142857142857, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI2367345, Length=103, Percent_Identity=30.0970873786408, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789370, Length=106, Percent_Identity=37.7358490566038, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI1787749, Length=87, Percent_Identity=34.4827586206897, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI226510944, Length=90, Percent_Identity=34.4444444444444, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1790326, Length=87, Percent_Identity=33.3333333333333, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR006547 [H]

Pfam domain/function: NA

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 57810; Mature: 57810

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVRAQIGMVLNLDKCIGCHTCSVTCKNVWTSRTGMEYAWFNNVETKPGIGYPKEWENQD
CCCCEEECEEEEHHHHCCCCEEEEEHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCC
KWNGGWIRKSDGSIEPRQGGKWKLLMRIFANPNMPQIDDYYEPFTFDYDHLQSAPEMKAA
CCCCCEEECCCCCCCCCCCCCCEEEEHHHCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCC
PTARPRSLITGKRMEKIEWGPNWEEILGGEFSKRSKDANLANFDQVQKDMVGQFENTFMM
CCCCCHHHHCCCHHHHCCCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
YLPRLCEHCLNPACVASCPSGSIYKREEDGIVLIDQDKCRGWRMCVSGCPYKKIYYNWKS
HHHHHHHHHCCCCEEECCCCCCCEEECCCCEEEEECCCCCCHHHHHCCCCCCCEEEEECC
GKAEKCTFCYPRIEAGQPTVCSETCVGRIRYLGVVLYDADRIQEAASVEDDKDLYQAQLD
CCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHEEEEECHHHHHHHHCCCCHHHHHEEEEE
IFLDPNDPKVIEQARKDGIPEAWLEGARRSPVYKMAVEWKVALPLHPEYRTLPMVWYVPP
EEECCCCCHHHHHHHHCCCCHHHHCCCCCCCEEEEEEEEEEEECCCCCCCCEEEEEEECC
LSPITSAANAGQIGINGELPDIAQMRIPVQYLANLLTAGDTAPVIRSLQRMLAMRSYQRS
CCHHHCCCCCCEECCCCCCCCHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
KHVDQVQNLAVLEQAGLSVAEVEEMYHIMAIANYEDRFVIPTTHREYAENAFDMRGGCGF
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHEEECCCCCEEECCCCHHHHHHHHHCCCCCCC
TFGNGCSDGASDVSLFGGNRKRTIPIKVEV
CCCCCCCCCCCCEEEECCCCCEEEEEEEEC
>Mature Secondary Structure
MKVRAQIGMVLNLDKCIGCHTCSVTCKNVWTSRTGMEYAWFNNVETKPGIGYPKEWENQD
CCCCEEECEEEEHHHHCCCCEEEEEHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCC
KWNGGWIRKSDGSIEPRQGGKWKLLMRIFANPNMPQIDDYYEPFTFDYDHLQSAPEMKAA
CCCCCEEECCCCCCCCCCCCCCEEEEHHHCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCC
PTARPRSLITGKRMEKIEWGPNWEEILGGEFSKRSKDANLANFDQVQKDMVGQFENTFMM
CCCCCHHHHCCCHHHHCCCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
YLPRLCEHCLNPACVASCPSGSIYKREEDGIVLIDQDKCRGWRMCVSGCPYKKIYYNWKS
HHHHHHHHHCCCCEEECCCCCCCEEECCCCEEEEECCCCCCHHHHHCCCCCCCEEEEECC
GKAEKCTFCYPRIEAGQPTVCSETCVGRIRYLGVVLYDADRIQEAASVEDDKDLYQAQLD
CCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHEEEEECHHHHHHHHCCCCHHHHHEEEEE
IFLDPNDPKVIEQARKDGIPEAWLEGARRSPVYKMAVEWKVALPLHPEYRTLPMVWYVPP
EEECCCCCHHHHHHHHCCCCHHHHCCCCCCCEEEEEEEEEEEECCCCCCCCEEEEEEECC
LSPITSAANAGQIGINGELPDIAQMRIPVQYLANLLTAGDTAPVIRSLQRMLAMRSYQRS
CCHHHCCCCCCEECCCCCCCCHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
KHVDQVQNLAVLEQAGLSVAEVEEMYHIMAIANYEDRFVIPTTHREYAENAFDMRGGCGF
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHEEECCCCCEEECCCCHHHHHHHHHCCCCCCC
TFGNGCSDGASDVSLFGGNRKRTIPIKVEV
CCCCCCCCCCCCEEEECCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2674654; 8905232; 9278503; 2832376; 3053688; 1321049; 8383531; 8388253; 8664273; 9516445; 12910261 [H]