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The map label for this gene is yafJ [C]

Identifier: 91790371

GI number: 91790371

Start: 4800998

End: 4801786

Strand: Direct

Name: yafJ [C]

Synonym: Bpro_4539

Alternate gene names: 91790371

Gene position: 4800998-4801786 (Clockwise)

Preceding gene: 91790370

Following gene: 91790379

Centisome position: 92.32

GC content: 63.62

Gene sequence:

>789_bases
ATGTGCCAATTGCTGGGAATGAACTGCAACACGCCGACGGACGTGACCTTCAGCTTTCGTGGTTTTGCGCAGCGTGGCGG
GCACACCGACCAGCACGCCGACGGCTGGGGCATTGCATTTTTTGAAGGCTCGGGCAGTGCAGACAGTGACAAGGGCCTGC
GCCATTTCGTCGACCACCAGCCGGCCTGCGAGTCGCCGGTGGCAGACCTGATTCGCCGCTACCCGATCAAAAGCCGCAAC
GTCATCGCGCACATCCGCAAGGCCACGCAGGGCCGGGTGGCGCTGGAAAACTGCCACCCCTTTGTGCGCGAGTTGTGGGG
CCGCTACTGGGTGTTTGCGCACAATGGCGACCTGAAAGATTTTGCGCCGCGGCTGCATGGCAGTTTCCGGCCCGTCGGCA
CCACCGATAGCGAACGGGCTTTTTGCTGGATCATGCAGGAGTTGGCCAAGTCCCATGCCGGCGTGCCCAGCGTGCGGGAG
CTGAGCATCACGCTGCGCGAGCTGGCGGCGCAGATTGCGCCGCACGGCACCTTCAATTTCCTGATGAGCAACGGCCAGGC
GCTGTGGGCCCATGCGTCGACCAGTCTGTGCTACGTGGAGCGCCGCCACCCGTTTACCCATGCCACCCTGAGCGACGAAG
ACGTGAGCGTCAACTTTGCCGAGCAAACCAGCCCGAACGACCGGGTGGCCGTGGTCGTCACGGCGCCGCTCACCACCAAC
GAAAACTGGACGGCTTTTGCCCCCGGCGAACTCAAGGTGTTCGAGGACGGCGCTCTGATCAACGCCTGA

Upstream 100 bases:

>100_bases
TGGCTGCCCTGTGCGCGCTGGCCATGGTGGCGGCCTGGCAACTCAAGGCGCCGGCACCTGTGCCAGCGCCCTTGCCCCAG
CCCTCGAAGGCGGATTGACC

Downstream 100 bases:

>100_bases
TCAACGCCTGATCAGCGCCGGCAAGGGGTTGCCGGTCAACTTTTCAGCGCCTGCTCCAGGGCGTCGATAAACATTGCCGG
GACGTCAAAGCCGGTCTGGT

Product: class-II glutamine amidotransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MCQLLGMNCNTPTDVTFSFRGFAQRGGHTDQHADGWGIAFFEGSGSADSDKGLRHFVDHQPACESPVADLIRRYPIKSRN
VIAHIRKATQGRVALENCHPFVRELWGRYWVFAHNGDLKDFAPRLHGSFRPVGTTDSERAFCWIMQELAKSHAGVPSVRE
LSITLRELAAQIAPHGTFNFLMSNGQALWAHASTSLCYVERRHPFTHATLSDEDVSVNFAEQTSPNDRVAVVVTAPLTTN
ENWTAFAPGELKVFEDGALINA

Sequences:

>Translated_262_residues
MCQLLGMNCNTPTDVTFSFRGFAQRGGHTDQHADGWGIAFFEGSGSADSDKGLRHFVDHQPACESPVADLIRRYPIKSRN
VIAHIRKATQGRVALENCHPFVRELWGRYWVFAHNGDLKDFAPRLHGSFRPVGTTDSERAFCWIMQELAKSHAGVPSVRE
LSITLRELAAQIAPHGTFNFLMSNGQALWAHASTSLCYVERRHPFTHATLSDEDVSVNFAEQTSPNDRVAVVVTAPLTTN
ENWTAFAPGELKVFEDGALINA
>Mature_262_residues
MCQLLGMNCNTPTDVTFSFRGFAQRGGHTDQHADGWGIAFFEGSGSADSDKGLRHFVDHQPACESPVADLIRRYPIKSRN
VIAHIRKATQGRVALENCHPFVRELWGRYWVFAHNGDLKDFAPRLHGSFRPVGTTDSERAFCWIMQELAKSHAGVPSVRE
LSITLRELAAQIAPHGTFNFLMSNGQALWAHASTSLCYVERRHPFTHATLSDEDVSVNFAEQTSPNDRVAVVVTAPLTTN
ENWTAFAPGELKVFEDGALINA

Specific function: Unknown

COG id: COG0121

COG function: function code R; Predicted glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1786417, Length=255, Percent_Identity=50.1960784313725, Blast_Score=270, Evalue=6e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932 [H]

Pfam domain/function: PF00310 GATase_2 [H]

EC number: NA

Molecular weight: Translated: 29064; Mature: 29064

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCQLLGMNCNTPTDVTFSFRGFAQRGGHTDQHADGWGIAFFEGSGSADSDKGLRHFVDHQ
CCEEEECCCCCCCCEEEEECCHHHCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHCCC
PACESPVADLIRRYPIKSRNVIAHIRKATQGRVALENCHPFVRELWGRYWVFAHNGDLKD
CCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCCEEHHHCCHHHHHHCCCEEEEEECCCHHH
FAPRLHGSFRPVGTTDSERAFCWIMQELAKSHAGVPSVRELSITLRELAAQIAPHGTFNF
HHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEE
LMSNGQALWAHASTSLCYVERRHPFTHATLSDEDVSVNFAEQTSPNDRVAVVVTAPLTTN
EECCCCEEEEECCCEEEEEECCCCEEECEECCCCEEEEEHHCCCCCCCEEEEEEECEECC
ENWTAFAPGELKVFEDGALINA
CCEEEECCCCEEEEECCCEECC
>Mature Secondary Structure
MCQLLGMNCNTPTDVTFSFRGFAQRGGHTDQHADGWGIAFFEGSGSADSDKGLRHFVDHQ
CCEEEECCCCCCCCEEEEECCHHHCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHCCC
PACESPVADLIRRYPIKSRNVIAHIRKATQGRVALENCHPFVRELWGRYWVFAHNGDLKD
CCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCCEEHHHCCHHHHHHCCCEEEEEECCCHHH
FAPRLHGSFRPVGTTDSERAFCWIMQELAKSHAGVPSVRELSITLRELAAQIAPHGTFNF
HHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEE
LMSNGQALWAHASTSLCYVERRHPFTHATLSDEDVSVNFAEQTSPNDRVAVVVTAPLTTN
EECCCCEEEEECCCEEEEEECCCCEEECEECCCCEEEEEHHCCCCCCCEEEEEEECEECC
ENWTAFAPGELKVFEDGALINA
CCEEEECCCCEEEEECCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]