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The map label for this gene is pcm2

Identifier: 91790116

GI number: 91790116

Start: 4509635

End: 4510270

Strand: Direct

Name: pcm2

Synonym: Bpro_4278

Alternate gene names: 91790116

Gene position: 4509635-4510270 (Clockwise)

Preceding gene: 91790115

Following gene: 91790117

Centisome position: 86.72

GC content: 66.35

Gene sequence:

>636_bases
ATGATGGAGGAAATCACGGCGCTCGTTCGCGAGACCCGCACCGAGACCGGCAAGCCAGCCCTTGATGAGCGGGTGATGGC
GGTCATGGGGAAGGTGCCGCGACACGAGTTCGTTCCCGCTGACCAGCTTCCCCGTGCCTACCAGAACCGCCCCCTGCCGA
TCGGCCACGGGCAGACCATCTCGCAGCCTTACATCGTCGCGCTGATGACCGATCTGGCCAGGGTGGAACCCGGCCACAAG
GTGCTCGAGGTCGGCACCGGTTCGGGTTATCAGGCGGCGGTGATGGCGCACCTAGCGCGTGCCGTCTACACCATCGAGAT
CATCGAGCCGCTGGGGCTGCAAGCCCGGCAACGCCTGCAGAAGCTGGGCTACGACAACGTCCAGGTGCGGCTGGGCGACG
GCTACCACGGCTGGGAGGAACACGCGCCGTACGATGCCATTCTCGTCACCGCGGCGGCCAGCCACATTCCGCCGCCACTC
ATCCGCCAGCTCAAGGCCGGCGGCAGGATGGTGATTCCGGTGGGCGCAGCATTCATGGTCCAGCAACTGATGCTGGTCGA
GAAGAACCGCGATGGGACGGTGTCGACCCGGCAGATCCTGCCGGTGGCCTTCGTTCCGCTGACCGGCCAGCGCTAG

Upstream 100 bases:

>100_bases
GGCCACACCCACCGTCCGTGTTGTTGCCGCAGCGCTCCTCTTGGCCATGACAGGGGCACAGACCGCCGCAGCCGAAGACT
TCGCGTCCGGGCACCGGCGG

Downstream 100 bases:

>100_bases
GATGAAACCCACCCCCGTTGCTGGCTCACTGCGTGTAGCCGCCTCCCCCTTCGAGGGGGCAACACCAGCGGCCCGGCAGA
GCCGGTTCCGCGGTGTTCCC

Product: protein-L-isoaspartate O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase 2; Protein L-isoaspartyl methyltransferase 2; Protein-beta-aspartate methyltransferase 2; PIMT 2

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MMEEITALVRETRTETGKPALDERVMAVMGKVPRHEFVPADQLPRAYQNRPLPIGHGQTISQPYIVALMTDLARVEPGHK
VLEVGTGSGYQAAVMAHLARAVYTIEIIEPLGLQARQRLQKLGYDNVQVRLGDGYHGWEEHAPYDAILVTAAASHIPPPL
IRQLKAGGRMVIPVGAAFMVQQLMLVEKNRDGTVSTRQILPVAFVPLTGQR

Sequences:

>Translated_211_residues
MMEEITALVRETRTETGKPALDERVMAVMGKVPRHEFVPADQLPRAYQNRPLPIGHGQTISQPYIVALMTDLARVEPGHK
VLEVGTGSGYQAAVMAHLARAVYTIEIIEPLGLQARQRLQKLGYDNVQVRLGDGYHGWEEHAPYDAILVTAAASHIPPPL
IRQLKAGGRMVIPVGAAFMVQQLMLVEKNRDGTVSTRQILPVAFVPLTGQR
>Mature_211_residues
MMEEITALVRETRTETGKPALDERVMAVMGKVPRHEFVPADQLPRAYQNRPLPIGHGQTISQPYIVALMTDLARVEPGHK
VLEVGTGSGYQAAVMAHLARAVYTIEIIEPLGLQARQRLQKLGYDNVQVRLGDGYHGWEEHAPYDAILVTAAASHIPPPL
IRQLKAGGRMVIPVGAAFMVQQLMLVEKNRDGTVSTRQILPVAFVPLTGQR

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family

Homologues:

Organism=Homo sapiens, GI226530908, Length=199, Percent_Identity=35.1758793969849, Blast_Score=98, Evalue=5e-21,
Organism=Escherichia coli, GI1789100, Length=186, Percent_Identity=47.3118279569892, Blast_Score=166, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI71983477, Length=218, Percent_Identity=33.4862385321101, Blast_Score=87, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI193207222, Length=214, Percent_Identity=33.1775700934579, Blast_Score=78, Evalue=3e-15,
Organism=Drosophila melanogaster, GI17981723, Length=204, Percent_Identity=31.3725490196078, Blast_Score=95, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PIMT2_POLSJ (Q123X2)

Other databases:

- EMBL:   CP000316
- RefSeq:   YP_551068.1
- ProteinModelPortal:   Q123X2
- SMR:   Q123X2
- STRING:   Q123X2
- GeneID:   4013041
- GenomeReviews:   CP000316_GR
- KEGG:   pol:Bpro_4278
- NMPDR:   fig|296591.1.peg.4349
- eggNOG:   COG2518
- HOGENOM:   HBG699907
- OMA:   GYKATIS
- PhylomeDB:   Q123X2
- ProtClustDB:   CLSK773872
- BioCyc:   PSP296591:BPRO_4278-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00090
- InterPro:   IPR000682
- PANTHER:   PTHR11579
- TIGRFAMs:   TIGR00080

Pfam domain/function: PF01135 PCMT

EC number: =2.1.1.77

Molecular weight: Translated: 23226; Mature: 23226

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: PS01279 PCMT

Important sites: ACT_SITE 61-61

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMEEITALVRETRTETGKPALDERVMAVMGKVPRHEFVPADQLPRAYQNRPLPIGHGQTI
CHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCHHHCCHHHCCCCCCCCCCCCC
SQPYIVALMTDLARVEPGHKVLEVGTGSGYQAAVMAHLARAVYTIEIIEPLGLQARQRLQ
CCCHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
KLGYDNVQVRLGDGYHGWEEHAPYDAILVTAAASHIPPPLIRQLKAGGRMVIPVGAAFMV
HCCCCCEEEEECCCCCCHHHCCCHHHHHEEHHHHHCCHHHHHHHHCCCEEEEECCHHHHH
QQLMLVEKNRDGTVSTRQILPVAFVPLTGQR
HHHHHHHCCCCCCCCHHEECEEEEEECCCCC
>Mature Secondary Structure
MMEEITALVRETRTETGKPALDERVMAVMGKVPRHEFVPADQLPRAYQNRPLPIGHGQTI
CHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCHHHCCHHHCCCCCCCCCCCCC
SQPYIVALMTDLARVEPGHKVLEVGTGSGYQAAVMAHLARAVYTIEIIEPLGLQARQRLQ
CCCHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
KLGYDNVQVRLGDGYHGWEEHAPYDAILVTAAASHIPPPLIRQLKAGGRMVIPVGAAFMV
HCCCCCEEEEECCCCCCHHHCCCHHHHHEEHHHHHCCHHHHHHHHCCCEEEEECCHHHHH
QQLMLVEKNRDGTVSTRQILPVAFVPLTGQR
HHHHHHHCCCCCCCCHHEECEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA