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The map label for this gene is glyS [H]

Identifier: 91790040

GI number: 91790040

Start: 4419515

End: 4421710

Strand: Reverse

Name: glyS [H]

Synonym: Bpro_4202

Alternate gene names: 91790040

Gene position: 4421710-4419515 (Counterclockwise)

Preceding gene: 91790041

Following gene: 91790039

Centisome position: 85.03

GC content: 62.52

Gene sequence:

>2196_bases
ATGACGATGACTACCCAAAACCTTCTGGTTGAGCTGTTTGTGGAGGAGCTGCCGCCCAAGGCGCTCAAGAAGTTAGGTGA
TGCGTTTGCTGGCGTGCTGCTGGAGCAGCTCAAGGCGCAGGGCCTGACGACCGCTGGGTCGGTGCTCACGTCTTTCGCCT
CACCGCGCCGCCTTGCCGCGCATGTGACGGCTGTCGCTGTGCAGGCCGCCGACAAGGCTGTTTCGCAAAAACTCATGCCG
GTGACCGTCGGTCTCGACGCGTCGGGCCAGGCCACACCGGCGTTGCTGAAAAAGCTGCAGGCGCTGGGCGCCGATGCCTT
GGCTGTGGCGGGCCTGAAGCGCGTACCGGATGGCAAGGCCGAAGCACTTTTCTACAACAGCACTGTGATCGGCGCCACGC
TGGCGGAAGGCCTGCAGAAAGCCCTCCTCGAGGCGATTGCCAAACTGCCGATTCCCAAGGTCATGACCTACCAGCTGGCC
GATGGCTGGAGCGACGTGAAGTTTGTGCGTCCTGCGCACGGGCTGGTTGCACTGCACGGCAGCGATGTCGTCGCCATTGA
AGCGTTGGGCCTGAAGGCCGGCAACATCACGCACGGCCATCGGTTTGAGGCTGCGGTTGATCCGGTGGTGTTGAAGAACG
CTGACGGGTACGCGGCCACGCTGCAAAAGGATGGCGCCGTGATCGCCAGCTTTGCCGAACGCAAGGCCGAGATTGCCCGC
CAGCTGGCGGCCGCTGCTTCCAAGGTGGGCGGTGGCGCCCGGGCGATTGAAGACGACGCCTTGCTCGACGAGGTGACTGC
ACTGGTCGAGCGCCCCAACGTGCTGGTCTGCGAGTTCGAAAAGGAATTCCTCGACGTGCCGCAGGAGTGCCTGATCCTCA
CGATGAAGGCCAACCAGAAGTACTTCCCGCTGCTGGATGCGCAGGGCAAGCTGACCCACAAGTTCCTCGTGGTCAGCAAC
ATCAGCCCCACCGATGCGAGCGCGGTGATTGGCGGCAACGAGCGCGTCGTGCGTCCGCGCCTGGCCGATGCCAAATTCTT
CTTCGACCAGGACCGCAAGAAAACGCTGGCCTCGCGCGTGGCCGGGCTGGACAAGGTGGTTTATCACAACAAGCTGGGTT
CGCAAGGTGAGCGCGTCATACGCGTGAGCCATATCGCTTACGAAATTGGCAAGAAGCTTTATCCCTTGGATGATTCAACG
GCGATCCATGCTCAGTTGGCGGCACAACTCGCCAAGGCTGACCTTGTAACCGACATGGTGGGCGAATTCCCGGAATTGCA
AGGCACCATGGGCCGCTATTACGCGTTGAACGATGGTCTTCGGCCTGATATCGCCGACGCCATTGAAGATCACTATAAGC
CGCGTTTTGCCGGTGACGAGCTGCCGCGCAACAGCGTTGGCGTGATCGTGGCACTGGCCGACAAGCTGGAGACACTGGTG
GGCATGTTCGGCATCGGCAACCTGCCCACCGGCGACAAGGATCCGTTTGCGCTGCGCCGTCATGCACTGGGTGTTATTCG
CATGCTGACGGAAAAGGACTTGGCATTGGACATGAACGCATTGCTGCAGAGTGCAGTCGATGTTTTTGGCGGCGCCGTTT
ATACGGAAGGTGTTAGTCCAAGTGCGCCAGTGCCTGGCACAAAGGGCGTGCTATCAACTGGCGTTGTGATGGATGTTCAA
ACCCGCGAGAAAATCACCGATTTCATCTACGACCGCCTGGCCGGCTCCTTGCGCGAGCAGGGCTACAGCGCGCAGGAAGT
TGATGCCGTGCTGGCCCTGCGGCCGCAGCGCCTGGGCGATGTCGCCAAACGCCTGGCGGCCGTGCGCGCCTTCGCGGCAT
TGCCCGAGGCGCCGGCACTCGCCGCGGCCAACAAGCGTGTCGGCAACATCCTGAAGAAAGCCGATGGCACGGTGGAGGCC
AGCGTCAGGCCGGAACTCCTGCAGGAAGCGGCGGAACAGGCACTGGCCGAAGCGCTGCAGTCCGTCGGGCCGAAAGCTGA
TGCAGCTTTCACCGCCGGTGACTACGCGGCTTCCCTGCAGGGGCTGGCCGCCCTGAAAGCACCCGTCGACGCGTTCTTCG
ACGGCGTGATGGTCAATGCCGAAGACCCGGCTCTGCGCGCCAACCGCCTCGGCCTGCTGGCCACGCTGCACCAGGCCATG
AACCGCGTGGCCGACCTGTCCAAGCTGGCAACATGA

Upstream 100 bases:

>100_bases
CCGTGGCACAAAGCTACTATGAGAGCCGCGAACGGCTCGGCTTCCCGATGGCGCCCCGCGAGTGGGTGGAACAGATGACG
AAGAAGGCGGCGTGAGAGCG

Downstream 100 bases:

>100_bases
GCCCCCTCGCAGTCTGACCCTTCAATGCAAAGCACCATGAAACTCGTCATCCTTGACCGCGACGGCACCATCAACAGCGA
CAGCGACGACTTCGTCAAGA

Product: glycine--tRNA ligase

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 731; Mature: 730

Protein sequence:

>731_residues
MTMTTQNLLVELFVEELPPKALKKLGDAFAGVLLEQLKAQGLTTAGSVLTSFASPRRLAAHVTAVAVQAADKAVSQKLMP
VTVGLDASGQATPALLKKLQALGADALAVAGLKRVPDGKAEALFYNSTVIGATLAEGLQKALLEAIAKLPIPKVMTYQLA
DGWSDVKFVRPAHGLVALHGSDVVAIEALGLKAGNITHGHRFEAAVDPVVLKNADGYAATLQKDGAVIASFAERKAEIAR
QLAAAASKVGGGARAIEDDALLDEVTALVERPNVLVCEFEKEFLDVPQECLILTMKANQKYFPLLDAQGKLTHKFLVVSN
ISPTDASAVIGGNERVVRPRLADAKFFFDQDRKKTLASRVAGLDKVVYHNKLGSQGERVIRVSHIAYEIGKKLYPLDDST
AIHAQLAAQLAKADLVTDMVGEFPELQGTMGRYYALNDGLRPDIADAIEDHYKPRFAGDELPRNSVGVIVALADKLETLV
GMFGIGNLPTGDKDPFALRRHALGVIRMLTEKDLALDMNALLQSAVDVFGGAVYTEGVSPSAPVPGTKGVLSTGVVMDVQ
TREKITDFIYDRLAGSLREQGYSAQEVDAVLALRPQRLGDVAKRLAAVRAFAALPEAPALAAANKRVGNILKKADGTVEA
SVRPELLQEAAEQALAEALQSVGPKADAAFTAGDYAASLQGLAALKAPVDAFFDGVMVNAEDPALRANRLGLLATLHQAM
NRVADLSKLAT

Sequences:

>Translated_731_residues
MTMTTQNLLVELFVEELPPKALKKLGDAFAGVLLEQLKAQGLTTAGSVLTSFASPRRLAAHVTAVAVQAADKAVSQKLMP
VTVGLDASGQATPALLKKLQALGADALAVAGLKRVPDGKAEALFYNSTVIGATLAEGLQKALLEAIAKLPIPKVMTYQLA
DGWSDVKFVRPAHGLVALHGSDVVAIEALGLKAGNITHGHRFEAAVDPVVLKNADGYAATLQKDGAVIASFAERKAEIAR
QLAAAASKVGGGARAIEDDALLDEVTALVERPNVLVCEFEKEFLDVPQECLILTMKANQKYFPLLDAQGKLTHKFLVVSN
ISPTDASAVIGGNERVVRPRLADAKFFFDQDRKKTLASRVAGLDKVVYHNKLGSQGERVIRVSHIAYEIGKKLYPLDDST
AIHAQLAAQLAKADLVTDMVGEFPELQGTMGRYYALNDGLRPDIADAIEDHYKPRFAGDELPRNSVGVIVALADKLETLV
GMFGIGNLPTGDKDPFALRRHALGVIRMLTEKDLALDMNALLQSAVDVFGGAVYTEGVSPSAPVPGTKGVLSTGVVMDVQ
TREKITDFIYDRLAGSLREQGYSAQEVDAVLALRPQRLGDVAKRLAAVRAFAALPEAPALAAANKRVGNILKKADGTVEA
SVRPELLQEAAEQALAEALQSVGPKADAAFTAGDYAASLQGLAALKAPVDAFFDGVMVNAEDPALRANRLGLLATLHQAM
NRVADLSKLAT
>Mature_730_residues
TMTTQNLLVELFVEELPPKALKKLGDAFAGVLLEQLKAQGLTTAGSVLTSFASPRRLAAHVTAVAVQAADKAVSQKLMPV
TVGLDASGQATPALLKKLQALGADALAVAGLKRVPDGKAEALFYNSTVIGATLAEGLQKALLEAIAKLPIPKVMTYQLAD
GWSDVKFVRPAHGLVALHGSDVVAIEALGLKAGNITHGHRFEAAVDPVVLKNADGYAATLQKDGAVIASFAERKAEIARQ
LAAAASKVGGGARAIEDDALLDEVTALVERPNVLVCEFEKEFLDVPQECLILTMKANQKYFPLLDAQGKLTHKFLVVSNI
SPTDASAVIGGNERVVRPRLADAKFFFDQDRKKTLASRVAGLDKVVYHNKLGSQGERVIRVSHIAYEIGKKLYPLDDSTA
IHAQLAAQLAKADLVTDMVGEFPELQGTMGRYYALNDGLRPDIADAIEDHYKPRFAGDELPRNSVGVIVALADKLETLVG
MFGIGNLPTGDKDPFALRRHALGVIRMLTEKDLALDMNALLQSAVDVFGGAVYTEGVSPSAPVPGTKGVLSTGVVMDVQT
REKITDFIYDRLAGSLREQGYSAQEVDAVLALRPQRLGDVAKRLAAVRAFAALPEAPALAAANKRVGNILKKADGTVEAS
VRPELLQEAAEQALAEALQSVGPKADAAFTAGDYAASLQGLAALKAPVDAFFDGVMVNAEDPALRANRLGLLATLHQAMN
RVADLSKLAT

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=727, Percent_Identity=40.8528198074278, Blast_Score=489, Evalue=1e-139,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311 [H]

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 77419; Mature: 77288

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMTTQNLLVELFVEELPPKALKKLGDAFAGVLLEQLKAQGLTTAGSVLTSFASPRRLAA
CCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHH
HVTAVAVQAADKAVSQKLMPVTVGLDASGQATPALLKKLQALGADALAVAGLKRVPDGKA
HHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCC
EALFYNSTVIGATLAEGLQKALLEAIAKLPIPKVMTYQLADGWSDVKFVRPAHGLVALHG
CEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEHHCCCCCCCEEECCCCCEEEECC
SDVVAIEALGLKAGNITHGHRFEAAVDPVVLKNADGYAATLQKDGAVIASFAERKAEIAR
CCEEEEEEECCCCCCCCCCCCCHHHCCCEEEECCCCCEEEECCCCHHHHHHHHHHHHHHH
QLAAAASKVGGGARAIEDDALLDEVTALVERPNVLVCEFEKEFLDVPQECLILTMKANQK
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECHHHHCCCHHHEEEEEECCCC
YFPLLDAQGKLTHKFLVVSNISPTDASAVIGGNERVVRPRLADAKFFFDQDRKKTLASRV
CCCEECCCCCCEEEEEEEECCCCCCCCEEECCCCCEECCCCCCHHHHHCCHHHHHHHHHH
AGLDKVVYHNKLGSQGERVIRVSHIAYEIGKKLYPLDDSTAIHAQLAAQLAKADLVTDMV
HHHHHHHHHHCCCCCCCCEEHHHHHHHHHCCEECCCCCCHHHHHHHHHHHHHHHHHHHHH
GEFPELQGTMGRYYALNDGLRPDIADAIEDHYKPRFAGDELPRNSVGVIVALADKLETLV
HCCHHHHCCCCCEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEHHHHHHHHH
GMFGIGNLPTGDKDPFALRRHALGVIRMLTEKDLALDMNALLQSAVDVFGGAVYTEGVSP
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEECCCCC
SAPVPGTKGVLSTGVVMDVQTREKITDFIYDRLAGSLREQGYSAQEVDAVLALRPQRLGD
CCCCCCCCCHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHH
VAKRLAAVRAFAALPEAPALAAANKRVGNILKKADGTVEASVRPELLQEAAEQALAEALQ
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHH
SVGPKADAAFTAGDYAASLQGLAALKAPVDAFFDGVMVNAEDPALRANRLGLLATLHQAM
HCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHH
NRVADLSKLAT
HHHHHHHHHCC
>Mature Secondary Structure 
TMTTQNLLVELFVEELPPKALKKLGDAFAGVLLEQLKAQGLTTAGSVLTSFASPRRLAA
CCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHH
HVTAVAVQAADKAVSQKLMPVTVGLDASGQATPALLKKLQALGADALAVAGLKRVPDGKA
HHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCC
EALFYNSTVIGATLAEGLQKALLEAIAKLPIPKVMTYQLADGWSDVKFVRPAHGLVALHG
CEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEHHCCCCCCCEEECCCCCEEEECC
SDVVAIEALGLKAGNITHGHRFEAAVDPVVLKNADGYAATLQKDGAVIASFAERKAEIAR
CCEEEEEEECCCCCCCCCCCCCHHHCCCEEEECCCCCEEEECCCCHHHHHHHHHHHHHHH
QLAAAASKVGGGARAIEDDALLDEVTALVERPNVLVCEFEKEFLDVPQECLILTMKANQK
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECHHHHCCCHHHEEEEEECCCC
YFPLLDAQGKLTHKFLVVSNISPTDASAVIGGNERVVRPRLADAKFFFDQDRKKTLASRV
CCCEECCCCCCEEEEEEEECCCCCCCCEEECCCCCEECCCCCCHHHHHCCHHHHHHHHHH
AGLDKVVYHNKLGSQGERVIRVSHIAYEIGKKLYPLDDSTAIHAQLAAQLAKADLVTDMV
HHHHHHHHHHCCCCCCCCEEHHHHHHHHHCCEECCCCCCHHHHHHHHHHHHHHHHHHHHH
GEFPELQGTMGRYYALNDGLRPDIADAIEDHYKPRFAGDELPRNSVGVIVALADKLETLV
HCCHHHHCCCCCEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEHHHHHHHHH
GMFGIGNLPTGDKDPFALRRHALGVIRMLTEKDLALDMNALLQSAVDVFGGAVYTEGVSP
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEECCCCC
SAPVPGTKGVLSTGVVMDVQTREKITDFIYDRLAGSLREQGYSAQEVDAVLALRPQRLGD
CCCCCCCCCHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHH
VAKRLAAVRAFAALPEAPALAAANKRVGNILKKADGTVEASVRPELLQEAAEQALAEALQ
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHH
SVGPKADAAFTAGDYAASLQGLAALKAPVDAFFDGVMVNAEDPALRANRLGLLATLHQAM
HCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHH
NRVADLSKLAT
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA