Click here to switch to the map view.

The map label for this gene is aceK

Identifier: 91790026

GI number: 91790026

Start: 4400775

End: 4402652

Strand: Reverse

Name: aceK

Synonym: Bpro_4188

Alternate gene names: 91790026

Gene position: 4402652-4400775 (Counterclockwise)

Preceding gene: 91790027

Following gene: 91790025

Centisome position: 84.66

GC content: 59.27

Gene sequence:

>1878_bases
GTGTTTCCCCAGCGCCTTGACTCCACCATCGCCTACGACATTGCGAAAGCGATGATGGACGGGTTCAACCGCCACTACCA
GCTGTTTCGCACCGAGTCGGCGCGTGCCAAGCACCGTTTCGAAACCGCCGACTGGCATGGCCAGCAACGCGCCCAGCGCG
AGCGTATCGAGTTTTACGACCTGCGCGTGCGCGAAGCGTCGACGCGGCTGGAGCGTGAGTTCAAGGCCGGCGAGCAGTCC
ATGGATGTCTGGCACCAGGTCAAGCTGCATTACATCGGCCTGCTGGTCAATCACCACCAGCCCGAACTGGCTGAGACCTT
CTTCAATTCGGTCACCACCAAAATACTGCACCGCAGCTATTTCCAGAACGACTTCATCTTCGTCCGGCCAGCCGTCAGCA
CCGAGTACATTGAAAACGAGGAGCCGACGGCGCAACCCACCTACCGTGCGTATTACCCGACCCGCGACAACCTGCGCGAG
ATCATCGTCCGGCTGGTGCGCGACTTCGACCTGCGCCTGGAGTTTGAAGACCTGGAGCGCGATGCCGGCTATGTGCTGGA
GGCCGTCAGCGAGCGGCTCAGCGACGTGAAACTGCGCGCCAATTTCCAGATACAGGTGTTGTCGGGCCTGTTTTTCCGCA
ACAAGGGCGCTTACGTCGTCGGCAAGATCATCAACGGCTTTAATGAAGTGCCGCTTGCCCTGCCGATATTGCACAGCAAG
AGCGGCAAGCTGGTGATAGACGCCGCACTGTTTGGCGAGGATGACCTGCTCATCCTGTTCAGTTTTGCGCGTGCCTACTT
CATGGTGGACATGGGCATCCCGTCGGCCTATGTGCAGTTTCTGCGCAGCATGATGCCGCACATGCCGCGCGCGGAAATCT
ACAACGCGCTCGGCCTGGCCAAGCAGGGCAAGACGCTGTTCTACCGAGACTTCCTTCACCACCTGCGGCACTCGACCGAC
AAGTTCCGCATTGCGCCCGGCATCAAGGGCATGGTGATGCTGGTGTTCGACCTGCCCTCGTTTCCTTATGTCTTCAAGGT
CATCAAGGACTACTATCCGCCGCAAAAAGACACCAGCCGCGAGCAGATCAAGGGCAAGTACCTGCTCGTCAAGCAGCATG
ACCGCGTGGGCCGCATGGCTGATACCCAGGAGTACAGTGAAGTTGCCTTTCCGCGCGCCCGGTTTGATGATGAGCTGATT
GCTGAAATTGAAAAGTTTGCACCCAGCCAGCTCGAAATCAGCGACCGCGACCGCGACGGCAACACGGAGGTGATCATCAA
GCACGTCTACATCGAGCGGCGCATGATTCCGCTGAACATTTACCTGCAGGAAGCGTTTGACGCCGGCGTCGGCGAGCCCG
CTGCAAAAAACCAGATCGAACGTGCCGTTGTCGAATACGGCAACGCCATCAAGGACCTGGTGGCAGCCAATATCTTTCCG
GGTGACATGCTGTGGAAGAACTTCGGCATCACCCGCCACGGCAAGGTCGTGTTCTATGACTACGACGAGATTGAATACAT
CACCGACTGCAAATTCCGCCGGGTGCCGGCGCCGCGCAATGATGAAGAGGAAATGAGCGGCGAGGTCTGGTATTCCGTGG
GGCCCAAAGACGTCTTTCCCGAAACCTTCGCCCCCTTCCTGCTCGGCAATGACGCGGTGCGCGAGGTTTTCATGAAGCAC
CACGGCGACTTGCTGGATGCCGAGTTCTGGCAAAGCCACCAGGCCCGCATTCAGGCCGGCCATGTGCACGACGTCTTCCC
CTATGAGCGGGAAAAGCGCTTCACCCGGCGCCATTCCCCCGGCCGGAATGACCACGAATTGTTGACCCACCTTCCGCCCG
AGCCGATGCTGACCGGCCTCAGTGGCATGACCCCCTAA

Upstream 100 bases:

>100_bases
CTGGATGCCCGCTACCGGCAGGCGGTGGACCGCGTCTGGGACCTCTGGAACAGCCGGTAACGTCAGATAACGCCAGACAG
CGCCAGAACCAGGACTCGCC

Downstream 100 bases:

>100_bases
GCGCGGCAAGAGCCTGGCAAGCGCGCAAAAAGCACGGCAAGAACAGCACCACAGTTTTCATTCAACCCTGAAAGGACACA
CATCATGTCGGATTCCATCG

Product: bifunctional isocitrate dehydrogenase kinase/phosphatase protein

Products: NA

Alternate protein names: IDH kinase/phosphatase; IDHK/P

Number of amino acids: Translated: 625; Mature: 625

Protein sequence:

>625_residues
MFPQRLDSTIAYDIAKAMMDGFNRHYQLFRTESARAKHRFETADWHGQQRAQRERIEFYDLRVREASTRLEREFKAGEQS
MDVWHQVKLHYIGLLVNHHQPELAETFFNSVTTKILHRSYFQNDFIFVRPAVSTEYIENEEPTAQPTYRAYYPTRDNLRE
IIVRLVRDFDLRLEFEDLERDAGYVLEAVSERLSDVKLRANFQIQVLSGLFFRNKGAYVVGKIINGFNEVPLALPILHSK
SGKLVIDAALFGEDDLLILFSFARAYFMVDMGIPSAYVQFLRSMMPHMPRAEIYNALGLAKQGKTLFYRDFLHHLRHSTD
KFRIAPGIKGMVMLVFDLPSFPYVFKVIKDYYPPQKDTSREQIKGKYLLVKQHDRVGRMADTQEYSEVAFPRARFDDELI
AEIEKFAPSQLEISDRDRDGNTEVIIKHVYIERRMIPLNIYLQEAFDAGVGEPAAKNQIERAVVEYGNAIKDLVAANIFP
GDMLWKNFGITRHGKVVFYDYDEIEYITDCKFRRVPAPRNDEEEMSGEVWYSVGPKDVFPETFAPFLLGNDAVREVFMKH
HGDLLDAEFWQSHQARIQAGHVHDVFPYEREKRFTRRHSPGRNDHELLTHLPPEPMLTGLSGMTP

Sequences:

>Translated_625_residues
MFPQRLDSTIAYDIAKAMMDGFNRHYQLFRTESARAKHRFETADWHGQQRAQRERIEFYDLRVREASTRLEREFKAGEQS
MDVWHQVKLHYIGLLVNHHQPELAETFFNSVTTKILHRSYFQNDFIFVRPAVSTEYIENEEPTAQPTYRAYYPTRDNLRE
IIVRLVRDFDLRLEFEDLERDAGYVLEAVSERLSDVKLRANFQIQVLSGLFFRNKGAYVVGKIINGFNEVPLALPILHSK
SGKLVIDAALFGEDDLLILFSFARAYFMVDMGIPSAYVQFLRSMMPHMPRAEIYNALGLAKQGKTLFYRDFLHHLRHSTD
KFRIAPGIKGMVMLVFDLPSFPYVFKVIKDYYPPQKDTSREQIKGKYLLVKQHDRVGRMADTQEYSEVAFPRARFDDELI
AEIEKFAPSQLEISDRDRDGNTEVIIKHVYIERRMIPLNIYLQEAFDAGVGEPAAKNQIERAVVEYGNAIKDLVAANIFP
GDMLWKNFGITRHGKVVFYDYDEIEYITDCKFRRVPAPRNDEEEMSGEVWYSVGPKDVFPETFAPFLLGNDAVREVFMKH
HGDLLDAEFWQSHQARIQAGHVHDVFPYEREKRFTRRHSPGRNDHELLTHLPPEPMLTGLSGMTP
>Mature_625_residues
MFPQRLDSTIAYDIAKAMMDGFNRHYQLFRTESARAKHRFETADWHGQQRAQRERIEFYDLRVREASTRLEREFKAGEQS
MDVWHQVKLHYIGLLVNHHQPELAETFFNSVTTKILHRSYFQNDFIFVRPAVSTEYIENEEPTAQPTYRAYYPTRDNLRE
IIVRLVRDFDLRLEFEDLERDAGYVLEAVSERLSDVKLRANFQIQVLSGLFFRNKGAYVVGKIINGFNEVPLALPILHSK
SGKLVIDAALFGEDDLLILFSFARAYFMVDMGIPSAYVQFLRSMMPHMPRAEIYNALGLAKQGKTLFYRDFLHHLRHSTD
KFRIAPGIKGMVMLVFDLPSFPYVFKVIKDYYPPQKDTSREQIKGKYLLVKQHDRVGRMADTQEYSEVAFPRARFDDELI
AEIEKFAPSQLEISDRDRDGNTEVIIKHVYIERRMIPLNIYLQEAFDAGVGEPAAKNQIERAVVEYGNAIKDLVAANIFP
GDMLWKNFGITRHGKVVFYDYDEIEYITDCKFRRVPAPRNDEEEMSGEVWYSVGPKDVFPETFAPFLLGNDAVREVFMKH
HGDLLDAEFWQSHQARIQAGHVHDVFPYEREKRFTRRHSPGRNDHELLTHLPPEPMLTGLSGMTP

Specific function: Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source

COG id: COG4579

COG function: function code T; Isocitrate dehydrogenase kinase/phosphatase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AceK family

Homologues:

Organism=Escherichia coli, GI1790446, Length=588, Percent_Identity=40.8163265306122, Blast_Score=441, Evalue=1e-125,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ACEK_POLSJ (Q124G2)

Other databases:

- EMBL:   CP000316
- RefSeq:   YP_550978.1
- STRING:   Q124G2
- GeneID:   4013128
- GenomeReviews:   CP000316_GR
- KEGG:   pol:Bpro_4188
- NMPDR:   fig|296591.1.peg.4260
- eggNOG:   COG4579
- HOGENOM:   HBG298593
- OMA:   RMTPLNI
- PhylomeDB:   Q124G2
- ProtClustDB:   PRK02946
- BioCyc:   PSP296591:BPRO_4188-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00747
- InterPro:   IPR010452
- PIRSF:   PIRSF000719
- ProDom:   PD043552

Pfam domain/function: PF06315 AceK

EC number: =2.7.11.5

Molecular weight: Translated: 72903; Mature: 72903

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: NA

Important sites: ACT_SITE 381-381 BINDING 346-346

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFPQRLDSTIAYDIAKAMMDGFNRHYQLFRTESARAKHRFETADWHGQQRAQRERIEFYD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHH
LRVREASTRLEREFKAGEQSMDVWHQVKLHYIGLLVNHHQPELAETFFNSVTTKILHRSY
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHH
FQNDFIFVRPAVSTEYIENEEPTAQPTYRAYYPTRDNLREIIVRLVRDFDLRLEFEDLER
HCCCEEEEECCCCHHHHCCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCEEEHHHHCC
DAGYVLEAVSERLSDVKLRANFQIQVLSGLFFRNKGAYVVGKIINGFNEVPLALPILHSK
HHHHHHHHHHHHHHCEEEECCEEEEEEHHHHCCCCCCEEEEHHHCCCCCCCEEEEEEECC
SGKLVIDAALFGEDDLLILFSFARAYFMVDMGIPSAYVQFLRSMMPHMPRAEIYNALGLA
CCCEEEEEEEECCCCHHHHHHHHHHHEEEECCCCHHHHHHHHHHCCCCCHHHHHHHHHHH
KQGKTLFYRDFLHHLRHSTDKFRIAPGIKGMVMLVFDLPSFPYVFKVIKDYYPPQKDTSR
HCCCCHHHHHHHHHHHCCCCCEEECCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCH
EQIKGKYLLVKQHDRVGRMADTQEYSEVAFPRARFDDELIAEIEKFAPSQLEISDRDRDG
HHHCCCEEEEECCCCCCCCCCHHHHHHHCCCHHCCCHHHHHHHHHCCCCCEEECCCCCCC
NTEVIIKHVYIERRMIPLNIYLQEAFDAGVGEPAAKNQIERAVVEYGNAIKDLVAANIFP
CCEEEEEEEEECCEEEEEEEEEEHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
GDMLWKNFGITRHGKVVFYDYDEIEYITDCKFRRVPAPRNDEEEMSGEVWYSVGPKDVFP
CHHHHHHCCCCCCCEEEEEECCCHHHHHCCCEECCCCCCCCHHHCCCCEEEECCCHHCCC
ETFAPFLLGNDAVREVFMKHHGDLLDAEFWQSHQARIQAGHVHDVFPYEREKRFTRRHSP
HHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHEECCCCCCCCCCHHHHHHHHHCCC
GRNDHELLTHLPPEPMLTGLSGMTP
CCCCHHHHHCCCCCHHHHCCCCCCC
>Mature Secondary Structure
MFPQRLDSTIAYDIAKAMMDGFNRHYQLFRTESARAKHRFETADWHGQQRAQRERIEFYD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHH
LRVREASTRLEREFKAGEQSMDVWHQVKLHYIGLLVNHHQPELAETFFNSVTTKILHRSY
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHH
FQNDFIFVRPAVSTEYIENEEPTAQPTYRAYYPTRDNLREIIVRLVRDFDLRLEFEDLER
HCCCEEEEECCCCHHHHCCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCEEEHHHHCC
DAGYVLEAVSERLSDVKLRANFQIQVLSGLFFRNKGAYVVGKIINGFNEVPLALPILHSK
HHHHHHHHHHHHHHCEEEECCEEEEEEHHHHCCCCCCEEEEHHHCCCCCCCEEEEEEECC
SGKLVIDAALFGEDDLLILFSFARAYFMVDMGIPSAYVQFLRSMMPHMPRAEIYNALGLA
CCCEEEEEEEECCCCHHHHHHHHHHHEEEECCCCHHHHHHHHHHCCCCCHHHHHHHHHHH
KQGKTLFYRDFLHHLRHSTDKFRIAPGIKGMVMLVFDLPSFPYVFKVIKDYYPPQKDTSR
HCCCCHHHHHHHHHHHCCCCCEEECCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCH
EQIKGKYLLVKQHDRVGRMADTQEYSEVAFPRARFDDELIAEIEKFAPSQLEISDRDRDG
HHHCCCEEEEECCCCCCCCCCHHHHHHHCCCHHCCCHHHHHHHHHCCCCCEEECCCCCCC
NTEVIIKHVYIERRMIPLNIYLQEAFDAGVGEPAAKNQIERAVVEYGNAIKDLVAANIFP
CCEEEEEEEEECCEEEEEEEEEEHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
GDMLWKNFGITRHGKVVFYDYDEIEYITDCKFRRVPAPRNDEEEMSGEVWYSVGPKDVFP
CHHHHHHCCCCCCCEEEEEECCCHHHHHCCCEECCCCCCCCHHHCCCCEEEECCCHHCCC
ETFAPFLLGNDAVREVFMKHHGDLLDAEFWQSHQARIQAGHVHDVFPYEREKRFTRRHSP
HHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHEECCCCCCCCCCHHHHHHHHHCCC
GRNDHELLTHLPPEPMLTGLSGMTP
CCCCHHHHHCCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA