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The map label for this gene is metF [H]

Identifier: 91789969

GI number: 91789969

Start: 4335384

End: 4336217

Strand: Reverse

Name: metF [H]

Synonym: Bpro_4130

Alternate gene names: 91789969

Gene position: 4336217-4335384 (Counterclockwise)

Preceding gene: 91789970

Following gene: 91789968

Centisome position: 83.38

GC content: 61.03

Gene sequence:

>834_bases
ATGAACCTTCCCATCAGTTTTGAATTCTTTCCTCCCAAGACGCCTGAGGGCGCAGACAAGCTGCGCGTGACCCGCCAGGC
CCTGTACGCGCACAAGCCGGAGTTTTGCTCCGTGACCTTTGGCGCGGGTGGCTCCACGCAAGAAGGCACCTTTGGCACCG
TCAAGGAAATCCTGGACGAAGGCGTGATCGCGGCCTGTCATTTCTCGTGCATTGGCGCGACCAAGATCACGGTGCGCCAG
CAGCTGGCGACCCTGCAGGCCATGGGCGTGAAGTACCTGGTCGCCCTGCGCGGTGATCTGCCCAGCGGCTATGGCATTGG
CGGTGAGTTTCATTACGCCAGCGACCTGGTGGCTTTCATCCGTGAAGAAACCGGAGACGCTTTTCACATTGAAGTGGCGG
CGTATCCTGAAGTTCACCCGCAGGCCAAGTCGGCCGATGCCGACCTGCAGGCTTTTGCCACCAAGGTCAGGGCCGGCGCC
AATTCGTCCATCACCCAGTACTTTTTCAACGCGGACGCCTACTTTCGGTTTGTGGACGATGTGCGCAAACTTGGCCTGGA
TGTGCCGGTGGTGCCCGGCATCATGCCCATTACCAGCTCCACGCAGCTCATGCGCTTTTCCGATGCCTGCGGCGCGGAAA
TCCCCCGCTGGATCCGCCTGCGGCTGCAGGGCTTCGGCGACGACACGGCGTCCATCAAGAGCTTTGGCCTCGACGTGGTC
ACCGAGCTGTGCGACCAGCTCTGCACGGCTGGCGTGCCCGGCATTCATTTTTATACGATGAACCAGGCCGCTGCGACGAC
GGAGATTCTTCGGCGTTTGCAGTACAGGGCCTGA

Upstream 100 bases:

>100_bases
CGAACCGCATGAAGACCCGGAAGCCGCGCATGCGGGCCAGCCGGGACCGGCGCGCGGCAAGCGGCGCGACAAGAAAACCG
ACAACGATTGAGATCTTGCG

Downstream 100 bases:

>100_bases
TGGCATGCAACACTGCACCAGGTAAGCCTGTGCACCAGGGCGCTGTCAGGGTCTGGATTGTTGCAAGCCTGCTGGCCGTG
CTGGCGGGCTGTGCGACAGC

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MNLPISFEFFPPKTPEGADKLRVTRQALYAHKPEFCSVTFGAGGSTQEGTFGTVKEILDEGVIAACHFSCIGATKITVRQ
QLATLQAMGVKYLVALRGDLPSGYGIGGEFHYASDLVAFIREETGDAFHIEVAAYPEVHPQAKSADADLQAFATKVRAGA
NSSITQYFFNADAYFRFVDDVRKLGLDVPVVPGIMPITSSTQLMRFSDACGAEIPRWIRLRLQGFGDDTASIKSFGLDVV
TELCDQLCTAGVPGIHFYTMNQAAATTEILRRLQYRA

Sequences:

>Translated_277_residues
MNLPISFEFFPPKTPEGADKLRVTRQALYAHKPEFCSVTFGAGGSTQEGTFGTVKEILDEGVIAACHFSCIGATKITVRQ
QLATLQAMGVKYLVALRGDLPSGYGIGGEFHYASDLVAFIREETGDAFHIEVAAYPEVHPQAKSADADLQAFATKVRAGA
NSSITQYFFNADAYFRFVDDVRKLGLDVPVVPGIMPITSSTQLMRFSDACGAEIPRWIRLRLQGFGDDTASIKSFGLDVV
TELCDQLCTAGVPGIHFYTMNQAAATTEILRRLQYRA
>Mature_277_residues
MNLPISFEFFPPKTPEGADKLRVTRQALYAHKPEFCSVTFGAGGSTQEGTFGTVKEILDEGVIAACHFSCIGATKITVRQ
QLATLQAMGVKYLVALRGDLPSGYGIGGEFHYASDLVAFIREETGDAFHIEVAAYPEVHPQAKSADADLQAFATKVRAGA
NSSITQYFFNADAYFRFVDDVRKLGLDVPVVPGIMPITSSTQLMRFSDACGAEIPRWIRLRLQGFGDDTASIKSFGLDVV
TELCDQLCTAGVPGIHFYTMNQAAATTEILRRLQYRA

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=277, Percent_Identity=38.9891696750903, Blast_Score=181, Evalue=5e-46,
Organism=Escherichia coli, GI1790377, Length=275, Percent_Identity=41.0909090909091, Blast_Score=209, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI25147441, Length=276, Percent_Identity=34.7826086956522, Blast_Score=164, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI25147436, Length=276, Percent_Identity=34.7826086956522, Blast_Score=164, Evalue=6e-41,
Organism=Saccharomyces cerevisiae, GI6321313, Length=280, Percent_Identity=32.8571428571429, Blast_Score=157, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6325234, Length=284, Percent_Identity=34.5070422535211, Blast_Score=144, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 30181; Mature: 30181

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLPISFEFFPPKTPEGADKLRVTRQALYAHKPEFCSVTFGAGGSTQEGTFGTVKEILDE
CCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHC
GVIAACHFSCIGATKITVRQQLATLQAMGVKYLVALRGDLPSGYGIGGEFHYASDLVAFI
CCEEEHHHHHHCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCHHHHHHHHHH
REETGDAFHIEVAAYPEVHPQAKSADADLQAFATKVRAGANSSITQYFFNADAYFRFVDD
HHCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHEECCHHHHHHHHH
VRKLGLDVPVVPGIMPITSSTQLMRFSDACGAEIPRWIRLRLQGFGDDTASIKSFGLDVV
HHHCCCCCCCCCCEEECCCCCHHEEECCCCCCCCCHHHHHEECCCCCCHHHHHHHHHHHH
TELCDQLCTAGVPGIHFYTMNQAAATTEILRRLQYRA
HHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNLPISFEFFPPKTPEGADKLRVTRQALYAHKPEFCSVTFGAGGSTQEGTFGTVKEILDE
CCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHC
GVIAACHFSCIGATKITVRQQLATLQAMGVKYLVALRGDLPSGYGIGGEFHYASDLVAFI
CCEEEHHHHHHCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCHHHHHHHHHH
REETGDAFHIEVAAYPEVHPQAKSADADLQAFATKVRAGANSSITQYFFNADAYFRFVDD
HHCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHEECCHHHHHHHHH
VRKLGLDVPVVPGIMPITSSTQLMRFSDACGAEIPRWIRLRLQGFGDDTASIKSFGLDVV
HHHCCCCCCCCCCEEECCCCCHHEEECCCCCCCCCHHHHHEECCCCCCHHHHHHHHHHHH
TELCDQLCTAGVPGIHFYTMNQAAATTEILRRLQYRA
HHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]