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The map label for this gene is rfbF [H]

Identifier: 91789849

GI number: 91789849

Start: 4208229

End: 4209002

Strand: Direct

Name: rfbF [H]

Synonym: Bpro_4009

Alternate gene names: 91789849

Gene position: 4208229-4209002 (Clockwise)

Preceding gene: 91789848

Following gene: 91789850

Centisome position: 80.92

GC content: 52.45

Gene sequence:

>774_bases
ATGAAAGCAGTCATCCTCGCAGGCGGTTTAGGGACCCGCATTTCCGAAGAAACCCACCTCAAACCGAAACCCATGGTGGA
AATCGGCGGGAAGCCCATACTCTGGCACATCATGAAGACCTATTCGGCTCATGGCGTAAACGATTTCATTATCTGCTGCG
GCTACAAGGGTTATGTCATCAAGGAGTATTTCGCCAATTACTTCCTTCATATGTCAGATGTCACTTTCGACATGGTCAAC
AATCAAATGCAGGTTCATCATCGCTATGCGGAGCCTTGGCGCGTGACCCTCGTGGATACTGGCGAAGAAACCATGACTGG
CGGTCGCCTCAAGCGCGTCGCACCTTATTTGCAAGGAGAAGACGCTTTCTGCTTCACTTATGGCGATGGTGTAAGTGACG
TAAACGTCAGTGAACTCATTTCTTTTCACAAAGCGCAAAGCGTCATGGCCACACTCACCGCTACGTTGCCTCCGGGCCGC
TTTGGCGCGCTGGATTTTGAGGGCAACAAGGTACGCACCTTCAAGGAAAAACCCAAGGGCGACGGAGCCATGATCAACGG
CGGTTTTTTTGTGTTGTCCCCCAAGGTGATCGATTACATCGAAAACGACGCTACCGTCTGGGAGCGTGACCCACTGGAAA
AACTGGCTGAAGAGGGCCAACTCGCCGCCTTCCAGCACGACGGTTTCTGGCAACCCATGGACACTCTGCGGGACAAAATT
CACTTGGAAGACTTGTGGTCTAGCGGCAAGGCGCCGTGGAAGGTGTGGAAATGA

Upstream 100 bases:

>100_bases
TTCATTCTTCTCTTTGGCATAGTGGGTTGGCAGGCCTGGATAGGGCGTCGCTCATTTCGGAGTTTTTTAATACAGAAAAA
TCAGGTAGGAATCTAGAACC

Downstream 100 bases:

>100_bases
ACCCAGCCTTCTGGCACGGCAGACGCGTCTTTCTCACTGGGCACACCGGCTTCAAGGGCAGCTGGATGTCCCTGTGGCTT
CAATCTCTAGGTGCGAACCT

Product: nucleotidyl transferase

Products: NA

Alternate protein names: CDP-glucose pyrophosphorylase [H]

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MKAVILAGGLGTRISEETHLKPKPMVEIGGKPILWHIMKTYSAHGVNDFIICCGYKGYVIKEYFANYFLHMSDVTFDMVN
NQMQVHHRYAEPWRVTLVDTGEETMTGGRLKRVAPYLQGEDAFCFTYGDGVSDVNVSELISFHKAQSVMATLTATLPPGR
FGALDFEGNKVRTFKEKPKGDGAMINGGFFVLSPKVIDYIENDATVWERDPLEKLAEEGQLAAFQHDGFWQPMDTLRDKI
HLEDLWSSGKAPWKVWK

Sequences:

>Translated_257_residues
MKAVILAGGLGTRISEETHLKPKPMVEIGGKPILWHIMKTYSAHGVNDFIICCGYKGYVIKEYFANYFLHMSDVTFDMVN
NQMQVHHRYAEPWRVTLVDTGEETMTGGRLKRVAPYLQGEDAFCFTYGDGVSDVNVSELISFHKAQSVMATLTATLPPGR
FGALDFEGNKVRTFKEKPKGDGAMINGGFFVLSPKVIDYIENDATVWERDPLEKLAEEGQLAAFQHDGFWQPMDTLRDKI
HLEDLWSSGKAPWKVWK
>Mature_257_residues
MKAVILAGGLGTRISEETHLKPKPMVEIGGKPILWHIMKTYSAHGVNDFIICCGYKGYVIKEYFANYFLHMSDVTFDMVN
NQMQVHHRYAEPWRVTLVDTGEETMTGGRLKRVAPYLQGEDAFCFTYGDGVSDVNVSELISFHKAQSVMATLTATLPPGR
FGALDFEGNKVRTFKEKPKGDGAMINGGFFVLSPKVIDYIENDATVWERDPLEKLAEEGQLAAFQHDGFWQPMDTLRDKI
HLEDLWSSGKAPWKVWK

Specific function: Involved in the biosynthesis of the tyvelose, a 3,6- dideoxyhexose found in the O-antigen of the surface lipopolysaccharides. It catalyzes the transfer of a CMP moiety from CTP to glucose 1-phosphate. This enzyme can utilize either CTP or UTP as the nucle

COG id: COG1208

COG function: function code MJ; Nucleoside-diphosphate-sugar pyrophosphorylase involved in lipopolysaccharide biosynthesis/translation initiation factor 2B, gamma/epsilon subunits (eIF-2Bgamma/eIF-2Bepsilon)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate cytidylyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI11761621, Length=246, Percent_Identity=28.8617886178862, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI11761619, Length=246, Percent_Identity=28.8617886178862, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI133931050, Length=248, Percent_Identity=29.8387096774194, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6320148, Length=247, Percent_Identity=31.5789473684211, Blast_Score=105, Evalue=8e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013446
- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.33 [H]

Molecular weight: Translated: 29029; Mature: 29029

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAVILAGGLGTRISEETHLKPKPMVEIGGKPILWHIMKTYSAHGVNDFIICCGYKGYVI
CCEEEEECCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHCCCCCCEEEEECCCCHHH
KEYFANYFLHMSDVTFDMVNNQMQVHHRYAEPWRVTLVDTGEETMTGGRLKRVAPYLQGE
HHHHHHHEEEECCEEEEHHCCCCEEHEECCCCEEEEEEECCCHHHCCCCHHHHHHHHCCC
DAFCFTYGDGVSDVNVSELISFHKAQSVMATLTATLPPGRFGALDFEGNKVRTFKEKPKG
CEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCEEEEECCCCEEEEECCCCCC
DGAMINGGFFVLSPKVIDYIENDATVWERDPLEKLAEEGQLAAFQHDGFWQPMDTLRDKI
CCEEEECCEEEECCHHHHHHCCCCCCCCCCHHHHHHCCCCEEEEECCCCCCCHHHHHHHH
HLEDLWSSGKAPWKVWK
HHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKAVILAGGLGTRISEETHLKPKPMVEIGGKPILWHIMKTYSAHGVNDFIICCGYKGYVI
CCEEEEECCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHCCCCCCEEEEECCCCHHH
KEYFANYFLHMSDVTFDMVNNQMQVHHRYAEPWRVTLVDTGEETMTGGRLKRVAPYLQGE
HHHHHHHEEEECCEEEEHHCCCCEEHEECCCCEEEEEEECCCHHHCCCCHHHHHHHHCCC
DAFCFTYGDGVSDVNVSELISFHKAQSVMATLTATLPPGRFGALDFEGNKVRTFKEKPKG
CEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCEEEEECCCCEEEEECCCCCC
DGAMINGGFFVLSPKVIDYIENDATVWERDPLEKLAEEGQLAAFQHDGFWQPMDTLRDKI
CCEEEECCEEEECCHHHHHHCCCCCCCCCCHHHHHHCCCCEEEEECCCCCCCHHHHHHHH
HLEDLWSSGKAPWKVWK
HHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]