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The map label for this gene is wcaJ [H]

Identifier: 91789825

GI number: 91789825

Start: 4178964

End: 4179524

Strand: Reverse

Name: wcaJ [H]

Synonym: Bpro_3985

Alternate gene names: 91789825

Gene position: 4179524-4178964 (Counterclockwise)

Preceding gene: 91789826

Following gene: 91789824

Centisome position: 80.37

GC content: 55.79

Gene sequence:

>561_bases
GTGAAGCGTCTGTTTGATTTGGTGCTGGCCCTGTGCGCCGCCGTGATTCTGCTGCTGCCATTAATGGTGGTGGCCCTGGC
GGTGCGCCTGACTTCGCCGGGGCCGGTCCTGTACTGGTCAGACCGGGTGGGCAAGGGCAACAGGATTTTCAAAATGCCCA
AATTTCGCAGCATGCGAATGGGCACGCCGGCTGTGGCCACGCATCTGCTGGAGGACCCCAAGGCCTATCTCACGCCCATA
GGTTCTTTTCTTCGAAAAAGCAGCCTGGACGAGTTACCGCAGTTGTGGAGCATTATCAGGGGCGATATGAGTTTTGTCGG
GCCCCGGCCCGCGTTGTTTAATCAGCATGATTTGATTGCCCTGCGTACCCAATATGGTGTGGATAAGCTCCTTCCCGGCT
TGACGGGCTGGGCTCAGGTGAATGGGCGGGACGAATTACCCGTTTTCCAAAAGGTCCAGCTTGATACCGAATACGTGCGG
CGCCAGTCGCTGTTTTTTGATATGAAGATAATCGGCCTGACTTTCTACCGGGTGATCAGGCGGGACAGTATTACCCATTA
A

Upstream 100 bases:

>100_bases
TCTTTGCAGGTCGACATCTCCAAAACACGGCAGTTGCTGGGGTGGCGTCCGCCGGTGTCAGTTGATGCGGGGCTGAAAAA
AGCCGCGGAGGGCTATTTGC

Downstream 100 bases:

>100_bases
TAATGAATTGCTGAAAATAATCATCCAGGCGAATTCGTGTGATTTAATATAAGGCACATTGTTTTAACAAGGAACGCGGC
CATGAGCAATCTGATCGATA

Product: sugar transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 186; Mature: 186

Protein sequence:

>186_residues
MKRLFDLVLALCAAVILLLPLMVVALAVRLTSPGPVLYWSDRVGKGNRIFKMPKFRSMRMGTPAVATHLLEDPKAYLTPI
GSFLRKSSLDELPQLWSIIRGDMSFVGPRPALFNQHDLIALRTQYGVDKLLPGLTGWAQVNGRDELPVFQKVQLDTEYVR
RQSLFFDMKIIGLTFYRVIRRDSITH

Sequences:

>Translated_186_residues
MKRLFDLVLALCAAVILLLPLMVVALAVRLTSPGPVLYWSDRVGKGNRIFKMPKFRSMRMGTPAVATHLLEDPKAYLTPI
GSFLRKSSLDELPQLWSIIRGDMSFVGPRPALFNQHDLIALRTQYGVDKLLPGLTGWAQVNGRDELPVFQKVQLDTEYVR
RQSLFFDMKIIGLTFYRVIRRDSITH
>Mature_186_residues
MKRLFDLVLALCAAVILLLPLMVVALAVRLTSPGPVLYWSDRVGKGNRIFKMPKFRSMRMGTPAVATHLLEDPKAYLTPI
GSFLRKSSLDELPQLWSIIRGDMSFVGPRPALFNQHDLIALRTQYGVDKLLPGLTGWAQVNGRDELPVFQKVQLDTEYVR
RQSLFFDMKIIGLTFYRVIRRDSITH

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=186, Percent_Identity=41.9354838709677, Blast_Score=127, Evalue=6e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 21171; Mature: 21171

Theoretical pI: Translated: 10.67; Mature: 10.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLFDLVLALCAAVILLLPLMVVALAVRLTSPGPVLYWSDRVGKGNRIFKMPKFRSMRM
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCEEEECCCCCCCCC
GTPAVATHLLEDPKAYLTPIGSFLRKSSLDELPQLWSIIRGDMSFVGPRPALFNQHDLIA
CCHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHCCCCCCCCCCCCEEE
LRTQYGVDKLLPGLTGWAQVNGRDELPVFQKVQLDTEYVRRQSLFFDMKIIGLTFYRVIR
EHHHHCHHHHCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDSITH
HHCCCC
>Mature Secondary Structure
MKRLFDLVLALCAAVILLLPLMVVALAVRLTSPGPVLYWSDRVGKGNRIFKMPKFRSMRM
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCEEEECCCCCCCCC
GTPAVATHLLEDPKAYLTPIGSFLRKSSLDELPQLWSIIRGDMSFVGPRPALFNQHDLIA
CCHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHCCCCCCCCCCCCEEE
LRTQYGVDKLLPGLTGWAQVNGRDELPVFQKVQLDTEYVRRQSLFFDMKIIGLTFYRVIR
EHHHHCHHHHCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDSITH
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]