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The map label for this gene is gcvA [H]

Identifier: 91789789

GI number: 91789789

Start: 4138920

End: 4139810

Strand: Reverse

Name: gcvA [H]

Synonym: Bpro_3949

Alternate gene names: 91789789

Gene position: 4139810-4138920 (Counterclockwise)

Preceding gene: 91789793

Following gene: 91789787

Centisome position: 79.61

GC content: 66.67

Gene sequence:

>891_bases
ATGCGCCGAAAAATCCCCTCCACCCAAGCCCTGCTGGCCTTCGAAGCGGCGGCGCACCGCGGCAGTTTTACGCTCGCGGG
GCAGGACCTGTCGGTGAGCCAGAGTGCGGTCAGCCATCAGATCCTGGGGCTGGAGCAGGACCTCGATGTGAGTCTTTTTT
TGCGCCTGCCGCGCCAGCTGGTGTTGACTGATGCCGGGCGCTCGCTGCTGTCGCGTGTCAGCCCGGCACTCGATGCGCTG
GAAGCCGCGATGCTGGACCTGGCGTCTTCCAAGGGCGAGGGCGGCACGCTGGAGCTCGGCGTGGTGCCCACCTTCGCCAC
CAAGTGGCTGATCCCCCGCATGCCCACCTTCCTCAAGGCGCACCCGCATGTGACGCTGAACCTGTCGACCCGGCTGCTGC
CCTTTGATTTTTCACTCACCGGCCTGGACGCGGCCATCCACTACGGCCGGCCCGACTGGCCGGGCACCGAGTCCGAGTAC
CTGATGGGCGAGGAAAGCGTGGTGGTGTGCAGCCCCGCGTTGATGAAAAAGCGCCAGCTGCGGCGCCCGGCCGACGTGCT
TAAGTTCACGCTGATCCACCAGAGCACGCGCCCGCACGCCTGGCGCGACTGGCTGCAGCTGGCCGGCATTGACGACGCGC
AGGGCGCCAGCACCGGCCCGAAGTACGAACTGTTTTCCATGATCGCCCAGGCCGTCAAGGCCGGGCTGGGCGTGGCCGTG
CTGCCGCGCTTTTTGGTGGCCGACGAGATTGCCGACGGCACCCTGGCCGTGCCGTTTGACCTGTCATTGAGTTCCGACTT
TGCCTACTATCTGGTGTGGCCCACGCAAAAGGCCAGCTGGCGGCCCCTGCTGCAGCTGCGCGAATGGCTGCGCGAACAGG
CGGCGCCTTAA

Upstream 100 bases:

>100_bases
TTCAGCGGGTAAATTCAACGGGTGGAAGCCGGATTTTCGGGAAATCACAGCCACTGCGCAAAGGATGTATTTCGATGCAG
GCATGAGATATATTCATGCG

Downstream 100 bases:

>100_bases
AGCGGCTCAGGCGCCGTCTGTAGGCGCCAGGCTTTGCGCCGGGCGGCGCAGCGTGCTGTCAAAAGGGTTGAGCTGCGCGC
CGATGGCCGTGGCCTCGCGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MRRKIPSTQALLAFEAAAHRGSFTLAGQDLSVSQSAVSHQILGLEQDLDVSLFLRLPRQLVLTDAGRSLLSRVSPALDAL
EAAMLDLASSKGEGGTLELGVVPTFATKWLIPRMPTFLKAHPHVTLNLSTRLLPFDFSLTGLDAAIHYGRPDWPGTESEY
LMGEESVVVCSPALMKKRQLRRPADVLKFTLIHQSTRPHAWRDWLQLAGIDDAQGASTGPKYELFSMIAQAVKAGLGVAV
LPRFLVADEIADGTLAVPFDLSLSSDFAYYLVWPTQKASWRPLLQLREWLREQAAP

Sequences:

>Translated_296_residues
MRRKIPSTQALLAFEAAAHRGSFTLAGQDLSVSQSAVSHQILGLEQDLDVSLFLRLPRQLVLTDAGRSLLSRVSPALDAL
EAAMLDLASSKGEGGTLELGVVPTFATKWLIPRMPTFLKAHPHVTLNLSTRLLPFDFSLTGLDAAIHYGRPDWPGTESEY
LMGEESVVVCSPALMKKRQLRRPADVLKFTLIHQSTRPHAWRDWLQLAGIDDAQGASTGPKYELFSMIAQAVKAGLGVAV
LPRFLVADEIADGTLAVPFDLSLSSDFAYYLVWPTQKASWRPLLQLREWLREQAAP
>Mature_296_residues
MRRKIPSTQALLAFEAAAHRGSFTLAGQDLSVSQSAVSHQILGLEQDLDVSLFLRLPRQLVLTDAGRSLLSRVSPALDAL
EAAMLDLASSKGEGGTLELGVVPTFATKWLIPRMPTFLKAHPHVTLNLSTRLLPFDFSLTGLDAAIHYGRPDWPGTESEY
LMGEESVVVCSPALMKKRQLRRPADVLKFTLIHQSTRPHAWRDWLQLAGIDDAQGASTGPKYELFSMIAQAVKAGLGVAV
LPRFLVADEIADGTLAVPFDLSLSSDFAYYLVWPTQKASWRPLLQLREWLREQAAP

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=296, Percent_Identity=34.4594594594595, Blast_Score=165, Evalue=3e-42,
Organism=Escherichia coli, GI1786448, Length=282, Percent_Identity=32.6241134751773, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1788706, Length=294, Percent_Identity=29.9319727891156, Blast_Score=121, Evalue=6e-29,
Organism=Escherichia coli, GI1786401, Length=254, Percent_Identity=26.7716535433071, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787589, Length=145, Percent_Identity=35.1724137931034, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787128, Length=134, Percent_Identity=32.8358208955224, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI157672245, Length=118, Percent_Identity=42.3728813559322, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI145693193, Length=287, Percent_Identity=25.4355400696864, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1789639, Length=253, Percent_Identity=28.4584980237154, Blast_Score=68, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32551; Mature: 32551

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRKIPSTQALLAFEAAAHRGSFTLAGQDLSVSQSAVSHQILGLEQDLDVSLFLRLPRQL
CCCCCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCHHHHHHCCHHH
VLTDAGRSLLSRVSPALDALEAAMLDLASSKGEGGTLELGVVPTFATKWLIPRMPTFLKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECHHHHHHHHCCCCHHHCC
HPHVTLNLSTRLLPFDFSLTGLDAAIHYGRPDWPGTESEYLMGEESVVVCSPALMKKRQL
CCCEEEEECCEEEEECCEECCHHHHEECCCCCCCCCCCCEEECCCCEEEECHHHHHHHHH
RRPADVLKFTLIHQSTRPHAWRDWLQLAGIDDAQGASTGPKYELFSMIAQAVKAGLGVAV
CCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHH
LPRFLVADEIADGTLAVPFDLSLSSDFAYYLVWPTQKASWRPLLQLREWLREQAAP
HHHHHHHHHCCCCEEEEEEECEECCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRRKIPSTQALLAFEAAAHRGSFTLAGQDLSVSQSAVSHQILGLEQDLDVSLFLRLPRQL
CCCCCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCHHHHHHCCHHH
VLTDAGRSLLSRVSPALDALEAAMLDLASSKGEGGTLELGVVPTFATKWLIPRMPTFLKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECHHHHHHHHCCCCHHHCC
HPHVTLNLSTRLLPFDFSLTGLDAAIHYGRPDWPGTESEYLMGEESVVVCSPALMKKRQL
CCCEEEEECCEEEEECCEECCHHHHEECCCCCCCCCCCCEEECCCCEEEECHHHHHHHHH
RRPADVLKFTLIHQSTRPHAWRDWLQLAGIDDAQGASTGPKYELFSMIAQAVKAGLGVAV
CCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHH
LPRFLVADEIADGTLAVPFDLSLSSDFAYYLVWPTQKASWRPLLQLREWLREQAAP
HHHHHHHHHCCCCEEEEEEECEECCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]