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The map label for this gene is ooxB [H]

Identifier: 91789782

GI number: 91789782

Start: 4132013

End: 4133170

Strand: Reverse

Name: ooxB [H]

Synonym: Bpro_3942

Alternate gene names: 91789782

Gene position: 4133170-4132013 (Counterclockwise)

Preceding gene: 91789783

Following gene: 91789781

Centisome position: 79.48

GC content: 69.26

Gene sequence:

>1158_bases
ATGATGGAACGCATCTCTGCCGACGTCGCCATCATTGGCGGCGGCATCATGGGCTCGTCGGCCGCGCTGTTCCTGCGCCG
CACGGGCCTGTCGGTGGTCCTGCTGGAGCGCGACCTCTGCGGCTCCCGCTCCAGCGGCGTGAACTATGGCGGCGTGCGGC
GCCAGGGGCGGCCGCCCAGCCAGTTGCCGCTGTCGCAACGCGCGCAGGGCTTGTGGGCGCAGTTGCCGCAACTCATCGGC
ATCGACGGCGAATATCTGCGCTCCGGCCACCTCAAGATTGCACGCAGCGAAGCTGATTTGGCTGCGCTGGAAAGTTACCG
CGAGCGCACGCAGGGCTTCGGCATGGACCTGCAAATCCTGTCGGCGCGGGCCCTGCGCGAGCGCTGCCCCTGGCTGGGCC
GGGCGGCTGTGGGCGCGTCGCTGTGCCCGGACGACGGGCAGGCCAACCCGCGCCTGGTGTCGCCGGCCTTTGCGCTGGCC
GCGCGGCGGCTGGGCGCGGATGTGCGCGAGCAGACATGCATCGACGAAGCGGCACACGACGGCACGGCGTTTGTGCTGCG
CTCGGGCCATGCGCTGGAAGTGCGCGCCAGGCATTTGCTGAACTGCGCGGGCGCTTGGGCCGGCACCATCGCGGCGCAGT
TTGGCGATGCGGTGCCCATGGAATCGGGCCACCCCGAGATGGCGGTGACCGAACCCCTGCCGGTGTTCCTCAACTTCAGC
CTGGGCGTGGAAGGCGGCGGCGTCTATGCGCGGCAGGTGGCGCGCGGCAATTGCGTCATTGGCGGCGGCCGGGGCTATGC
GCTGGACGAGCAGCGCGCCCGCGCCCAACGCAGCGGCATTGCCTCCCTGATGCAGCAGACCATTGACTTGCTGCCGGCCC
TGCGCAACGCGCACATCATCCGCACCTGGAGTGGCACCGAAGGCTACTTGCCCGACCGCCAGCCGGTGCTGGGCCCCAGC
GCCACCACACCCGGCCTGATTCATGCATTCGGTTTTGCCGGCGGCGGTTTTCAGCTGGGCCCCGCAGTCGGCGCCGTGCT
GGCCGACCTGGTGCGTGACGGCCAGACCGCCACACCGATTGATGCATTTTCTGTTTCGCGTTTTTCCGAAAGCCCGCGTG
TGGCAACCACCGGGCCTGTTCCTTCCGCCGTTGTCTAA

Upstream 100 bases:

>100_bases
CTTGCGCCGCCGCACCCGATGCGGTGGGCCGGCTGCGCGGCCAGGCGCCCATCAAGCCGCTGCCGTTCGCGGCCATGGCC
CGGGCGAACGGAGAGGCCGC

Downstream 100 bases:

>100_bases
CCCCTCGATGTTTAACCTTGTTTCCAGGAGAACCCCATGAAGCTGCCCTCACGTTTCCCGTCCGCCCGCGCCACCGCGGT
GTCCCTGGCCGTTGCGGCCG

Product: FAD dependent oxidoreductase

Products: glycine; formaldehyde; H2O2

Alternate protein names: Octopine oxidase subunit B [H]

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MMERISADVAIIGGGIMGSSAALFLRRTGLSVVLLERDLCGSRSSGVNYGGVRRQGRPPSQLPLSQRAQGLWAQLPQLIG
IDGEYLRSGHLKIARSEADLAALESYRERTQGFGMDLQILSARALRERCPWLGRAAVGASLCPDDGQANPRLVSPAFALA
ARRLGADVREQTCIDEAAHDGTAFVLRSGHALEVRARHLLNCAGAWAGTIAAQFGDAVPMESGHPEMAVTEPLPVFLNFS
LGVEGGGVYARQVARGNCVIGGGRGYALDEQRARAQRSGIASLMQQTIDLLPALRNAHIIRTWSGTEGYLPDRQPVLGPS
ATTPGLIHAFGFAGGGFQLGPAVGAVLADLVRDGQTATPIDAFSVSRFSESPRVATTGPVPSAVV

Sequences:

>Translated_385_residues
MMERISADVAIIGGGIMGSSAALFLRRTGLSVVLLERDLCGSRSSGVNYGGVRRQGRPPSQLPLSQRAQGLWAQLPQLIG
IDGEYLRSGHLKIARSEADLAALESYRERTQGFGMDLQILSARALRERCPWLGRAAVGASLCPDDGQANPRLVSPAFALA
ARRLGADVREQTCIDEAAHDGTAFVLRSGHALEVRARHLLNCAGAWAGTIAAQFGDAVPMESGHPEMAVTEPLPVFLNFS
LGVEGGGVYARQVARGNCVIGGGRGYALDEQRARAQRSGIASLMQQTIDLLPALRNAHIIRTWSGTEGYLPDRQPVLGPS
ATTPGLIHAFGFAGGGFQLGPAVGAVLADLVRDGQTATPIDAFSVSRFSESPRVATTGPVPSAVV
>Mature_385_residues
MMERISADVAIIGGGIMGSSAALFLRRTGLSVVLLERDLCGSRSSGVNYGGVRRQGRPPSQLPLSQRAQGLWAQLPQLIG
IDGEYLRSGHLKIARSEADLAALESYRERTQGFGMDLQILSARALRERCPWLGRAAVGASLCPDDGQANPRLVSPAFALA
ARRLGADVREQTCIDEAAHDGTAFVLRSGHALEVRARHLLNCAGAWAGTIAAQFGDAVPMESGHPEMAVTEPLPVFLNFS
LGVEGGGVYARQVARGNCVIGGGRGYALDEQRARAQRSGIASLMQQTIDLLPALRNAHIIRTWSGTEGYLPDRQPVLGPS
ATTPGLIHAFGFAGGGFQLGPAVGAVLADLVRDGQTATPIDAFSVSRFSESPRVATTGPVPSAVV

Specific function: Oxidative cleavage of octopine into L-arginine and pyruvate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI197927446, Length=385, Percent_Identity=24.4155844155844, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI21361378, Length=385, Percent_Identity=24.4155844155844, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI8923708, Length=393, Percent_Identity=24.4274809160305, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI194306651, Length=380, Percent_Identity=22.8947368421053, Blast_Score=76, Evalue=7e-14,
Organism=Homo sapiens, GI24797151, Length=392, Percent_Identity=23.469387755102, Blast_Score=75, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI32563613, Length=391, Percent_Identity=24.5524296675192, Blast_Score=76, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI71994045, Length=396, Percent_Identity=23.989898989899, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI20130091, Length=380, Percent_Identity=25.7894736842105, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI28571104, Length=394, Percent_Identity=24.6192893401015, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.5.3.1

Molecular weight: Translated: 40536; Mature: 40536

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMERISADVAIIGGGIMGSSAALFLRRTGLSVVLLERDLCGSRSSGVNYGGVRRQGRPPS
CCCCCCCCEEEEECCCCCCHHEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCC
QLPLSQRAQGLWAQLPQLIGIDGEYLRSGHLKIARSEADLAALESYRERTQGFGMDLQIL
CCCHHHHHHHHHHHHHHHHCCCHHHHHCCCEEEECCHHHHHHHHHHHHHHHCCCCCHHHH
SARALRERCPWLGRAAVGASLCPDDGQANPRLVSPAFALAARRLGADVREQTCIDEAAHD
HHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCEECHHHHHHHHHHCCHHHHHHHHHHHCCC
GTAFVLRSGHALEVRARHLLNCAGAWAGTIAAQFGDAVPMESGHPEMAVTEPLPVFLNFS
CEEEEEECCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCEEEEEE
LGVEGGGVYARQVARGNCVIGGGRGYALDEQRARAQRSGIASLMQQTIDLLPALRNAHII
ECCCCCCHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEE
RTWSGTEGYLPDRQPVLGPSATTPGLIHAFGFAGGGFQLGPAVGAVLADLVRDGQTATPI
EEECCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCC
DAFSVSRFSESPRVATTGPVPSAVV
HHHHHHHHCCCCCCEECCCCCCCCC
>Mature Secondary Structure
MMERISADVAIIGGGIMGSSAALFLRRTGLSVVLLERDLCGSRSSGVNYGGVRRQGRPPS
CCCCCCCCEEEEECCCCCCHHEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCC
QLPLSQRAQGLWAQLPQLIGIDGEYLRSGHLKIARSEADLAALESYRERTQGFGMDLQIL
CCCHHHHHHHHHHHHHHHHCCCHHHHHCCCEEEECCHHHHHHHHHHHHHHHCCCCCHHHH
SARALRERCPWLGRAAVGASLCPDDGQANPRLVSPAFALAARRLGADVREQTCIDEAAHD
HHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCEECHHHHHHHHHHCCHHHHHHHHHHHCCC
GTAFVLRSGHALEVRARHLLNCAGAWAGTIAAQFGDAVPMESGHPEMAVTEPLPVFLNFS
CEEEEEECCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCEEEEEE
LGVEGGGVYARQVARGNCVIGGGRGYALDEQRARAQRSGIASLMQQTIDLLPALRNAHII
ECCCCCCHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEE
RTWSGTEGYLPDRQPVLGPSATTPGLIHAFGFAGGGFQLGPAVGAVLADLVRDGQTATPI
EEECCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCC
DAFSVSRFSESPRVATTGPVPSAVV
HHHHHHHHCCCCCCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: sarcosine; H2O; O2

Specific reaction: sarcosine + H2O + O2 = glycine + formaldehyde + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA