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The map label for this gene is cca [H]

Identifier: 91789755

GI number: 91789755

Start: 4108136

End: 4109524

Strand: Direct

Name: cca [H]

Synonym: Bpro_3914

Alternate gene names: 91789755

Gene position: 4108136-4109524 (Clockwise)

Preceding gene: 91789754

Following gene: 91789756

Centisome position: 79.0

GC content: 69.11

Gene sequence:

>1389_bases
ATGCAGATTTACATGGTCGGTGGTGCAGTGCGCGATGCCTTGATGGGCCTGGCGGTGCAGGACCACGACTGGGTGGTGGT
GGGTGCGACACCGGAGGACCTGGTGGCCCAGGGCTATCAGCCGGTGGGCAAGGATTTTCCGGTGTTCTTGCACCCCGAAA
CCCGCGAGGAGTACGCGCTGGCCCGCACCGAGCGCAAGACCGCGCGCGGCTACCGCGGCTTTGTGGTGCATGCCGATCCC
GGCGTCACGCTGGAGCAGGATCTGGCACGGCGCGATCTCACCATCAATGCCATCGCGGCTCCCGCCCGTTCGACAGGTGC
TGCGGCTGGCGCTGCAGCCCGCCCCGGTGCCGCATTGAATACTGATTTTGACGCTGACTTTGACGCGCTGATCGACCCTT
ATGGCGGCCAGAAGGACATCCGGGACAAGGTCCTGCGCCACGTGACCGAGGCCTTCCGCGAAGACCCGGTGCGCATTCTC
CGGCTGGCCCGCTTTGCCGCCCGATTTGCGGATTTTTCGGTGGCGCCCGAAACCCTGGCGCTGATGCGGGACATGGTGAC
CGAGGGCGAAGTCGATGCACTGGTCCCCGAACGCGTCTGGCAGGAGCTGGCCCGCGGACTGATGGAGGACAAGCCCTCGC
GCCTCTTTGATGTGCTGCGCGACTGCGGCGCATTGCACAGGCTGCTGCCGGAAGTCGCCAGGCTCTGGGGCGTGCCGCAG
CGCGCCGAGCACCACCCCGAAGTCGACACCGGCGTCCACCTGATGATGGTGATGGACATGGCAGCGCAGCTCCAGGCGTC
CCTGCCCGTGCGCTTCGCCTGCCTGTGCCACGACCTGGGCAAGGGCACGACGCGGGCACCGGACGCTGGCATGTCCACGC
CAGGCGAGCCTCCCCCGCCAGAGTTCTGGCGCGAGAAGATGGCGTCCGACCCCCAGCTGGGACGCCACATTGGCCATGAG
GAGCGCAGTGCGCGCCTGCTCAAGGACGTATGCGAGCGCCTGCGCGTGCCGGTGGACTGCCGCGAGCTGGCCGATGTGGT
GGCGCGCGAGCACGGCAACATTCACGGCAGTGCCGCATTCGGCGCGGCGGCCGTGGTGCGCCTGCTGGAGCGCTGCGACG
CATTTCGCAAGCCCAGGCGCTTTGCCGAGGTGCTGCTGGCCTGCGAGTGCGATGCCCGTGGCCGTCTCGGGTACGAGCAT
TCGCCGTATCCCCAGCGCCCGCGGCTGCTGGAGGCCCTCGCCGCGGCCCAGTCTGTCGCCACGCACCAGGTGGCCGCCGA
CGCGCAGGCCGCCGGGCAGGATGGCAGGCAGATTGGCGAGCTGATTCACCAGGCCCGGGTGGCGGCCGTGGGCGAGATCC
CCGGCTTTGCGGCGGGCTCCCCCGGCTGA

Upstream 100 bases:

>100_bases
CGGCCCTGGACAGCAGCTTCATCCTGACCAAACAGCTGTGAACAGAAGAGCCACGCGGCCGCCCCTGCCGCGTGAGCCGG
GCAAAGCCTACACTTCGGTC

Downstream 100 bases:

>100_bases
TCTTTCGACCGAAAATTGTGAAAAAGCGAAGCCGGCCTACAGCCATGTCGCACGTGTTTTGCTAAGGTGAATGCCGATGC
AGCTTCTCACCCCATCCCCG

Product: metal dependent phosphohydrolase

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase [H]

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MQIYMVGGAVRDALMGLAVQDHDWVVVGATPEDLVAQGYQPVGKDFPVFLHPETREEYALARTERKTARGYRGFVVHADP
GVTLEQDLARRDLTINAIAAPARSTGAAAGAAARPGAALNTDFDADFDALIDPYGGQKDIRDKVLRHVTEAFREDPVRIL
RLARFAARFADFSVAPETLALMRDMVTEGEVDALVPERVWQELARGLMEDKPSRLFDVLRDCGALHRLLPEVARLWGVPQ
RAEHHPEVDTGVHLMMVMDMAAQLQASLPVRFACLCHDLGKGTTRAPDAGMSTPGEPPPPEFWREKMASDPQLGRHIGHE
ERSARLLKDVCERLRVPVDCRELADVVAREHGNIHGSAAFGAAAVVRLLERCDAFRKPRRFAEVLLACECDARGRLGYEH
SPYPQRPRLLEALAAAQSVATHQVAADAQAAGQDGRQIGELIHQARVAAVGEIPGFAAGSPG

Sequences:

>Translated_462_residues
MQIYMVGGAVRDALMGLAVQDHDWVVVGATPEDLVAQGYQPVGKDFPVFLHPETREEYALARTERKTARGYRGFVVHADP
GVTLEQDLARRDLTINAIAAPARSTGAAAGAAARPGAALNTDFDADFDALIDPYGGQKDIRDKVLRHVTEAFREDPVRIL
RLARFAARFADFSVAPETLALMRDMVTEGEVDALVPERVWQELARGLMEDKPSRLFDVLRDCGALHRLLPEVARLWGVPQ
RAEHHPEVDTGVHLMMVMDMAAQLQASLPVRFACLCHDLGKGTTRAPDAGMSTPGEPPPPEFWREKMASDPQLGRHIGHE
ERSARLLKDVCERLRVPVDCRELADVVAREHGNIHGSAAFGAAAVVRLLERCDAFRKPRRFAEVLLACECDARGRLGYEH
SPYPQRPRLLEALAAAQSVATHQVAADAQAAGQDGRQIGELIHQARVAAVGEIPGFAAGSPG
>Mature_462_residues
MQIYMVGGAVRDALMGLAVQDHDWVVVGATPEDLVAQGYQPVGKDFPVFLHPETREEYALARTERKTARGYRGFVVHADP
GVTLEQDLARRDLTINAIAAPARSTGAAAGAAARPGAALNTDFDADFDALIDPYGGQKDIRDKVLRHVTEAFREDPVRIL
RLARFAARFADFSVAPETLALMRDMVTEGEVDALVPERVWQELARGLMEDKPSRLFDVLRDCGALHRLLPEVARLWGVPQ
RAEHHPEVDTGVHLMMVMDMAAQLQASLPVRFACLCHDLGKGTTRAPDAGMSTPGEPPPPEFWREKMASDPQLGRHIGHE
ERSARLLKDVCERLRVPVDCRELADVVAREHGNIHGSAAFGAAAVVRLLERCDAFRKPRRFAEVLLACECDARGRLGYEH
SPYPQRPRLLEALAAAQSVATHQVAADAQAAGQDGRQIGELIHQARVAAVGEIPGFAAGSPG

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789436, Length=452, Percent_Identity=48.4513274336283, Blast_Score=400, Evalue=1e-112,
Organism=Escherichia coli, GI87081691, Length=248, Percent_Identity=28.2258064516129, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 50438; Mature: 50438

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIYMVGGAVRDALMGLAVQDHDWVVVGATPEDLVAQGYQPVGKDFPVFLHPETREEYAL
CEEEEECHHHHHHHHHCEEECCCEEEECCCHHHHHHCCCCCCCCCCCEEECCCCHHHHHH
ARTERKTARGYRGFVVHADPGVTLEQDLARRDLTINAIAAPARSTGAAAGAAARPGAALN
HHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCEEEHHHCCCCCCCCCCCCCCCCCCCCC
TDFDADFDALIDPYGGQKDIRDKVLRHVTEAFREDPVRILRLARFAARFADFSVAPETLA
CCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCHHHHH
LMRDMVTEGEVDALVPERVWQELARGLMEDKPSRLFDVLRDCGALHRLLPEVARLWGVPQ
HHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RAEHHPEVDTGVHLMMVMDMAAQLQASLPVRFACLCHDLGKGTTRAPDAGMSTPGEPPPP
HHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCH
EFWREKMASDPQLGRHIGHEERSARLLKDVCERLRVPVDCRELADVVAREHGNIHGSAAF
HHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHH
GAAAVVRLLERCDAFRKPRRFAEVLLACECDARGRLGYEHSPYPQRPRLLEALAAAQSVA
HHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
THQVAADAQAAGQDGRQIGELIHQARVAAVGEIPGFAAGSPG
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MQIYMVGGAVRDALMGLAVQDHDWVVVGATPEDLVAQGYQPVGKDFPVFLHPETREEYAL
CEEEEECHHHHHHHHHCEEECCCEEEECCCHHHHHHCCCCCCCCCCCEEECCCCHHHHHH
ARTERKTARGYRGFVVHADPGVTLEQDLARRDLTINAIAAPARSTGAAAGAAARPGAALN
HHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCEEEHHHCCCCCCCCCCCCCCCCCCCCC
TDFDADFDALIDPYGGQKDIRDKVLRHVTEAFREDPVRILRLARFAARFADFSVAPETLA
CCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCHHHHH
LMRDMVTEGEVDALVPERVWQELARGLMEDKPSRLFDVLRDCGALHRLLPEVARLWGVPQ
HHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RAEHHPEVDTGVHLMMVMDMAAQLQASLPVRFACLCHDLGKGTTRAPDAGMSTPGEPPPP
HHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCH
EFWREKMASDPQLGRHIGHEERSARLLKDVCERLRVPVDCRELADVVAREHGNIHGSAAF
HHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHH
GAAAVVRLLERCDAFRKPRRFAEVLLACECDARGRLGYEHSPYPQRPRLLEALAAAQSVA
HHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
THQVAADAQAAGQDGRQIGELIHQARVAAVGEIPGFAAGSPG
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA