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The map label for this gene is potB [H]

Identifier: 91789730

GI number: 91789730

Start: 4081068

End: 4081973

Strand: Direct

Name: potB [H]

Synonym: Bpro_3888

Alternate gene names: 91789730

Gene position: 4081068-4081973 (Clockwise)

Preceding gene: 91789729

Following gene: 91789731

Centisome position: 78.48

GC content: 62.58

Gene sequence:

>906_bases
ATGCAAACCGCGGTTGCCGCCCCGGGAATTGGCACAACACCAGCCTCAACGCAGGCCCGGCTTATCCTTTTCCTGTTGCT
GGCGCCTGCGCTGATCTGGCTGGTGGGCCTGATTGTGCTGCCGCACATCGATCTGGCGGTGCTGTCGCTGCGCGAGCGCA
TCGCGCCGCGGCAGTACACGCCCAGCATCGCCCAGTACCGCACCTTCATTGAAGAGCCGCTGTACTGGCACACCTTTGTG
CGGACAGCCGTCATGTCCATCCTGGCCACACTGATCACGCTGCTGCTGGCCTTTCCGGTGGCCTGGACCATTGCCAAGCT
GGTGCGCGGCCGCGCCAAGTCCTTGCTGCTGGTGCTGTGCCTGATCCCCTTCTGGGTCAGCGAAACCGTGCGCACCTTGG
GGTGGATGATCCTGCTGCGCGAGTCCGGCGTGCTGCCCGCCCTGCTGGTGCAACTCGGCCTCACGGCAACCCCTGTGGAA
ATGCTTTACCGGGATGCCACCATTCTGGTGGGCCTGGTGTACACCTCCATGCTGTTCATGGTGGTGCCGCTGATCAGCGC
CCTGGAAAGCCTGGACGACGCCCTGATTGAAGCGGCCTACGATCTGGGAGGCACCGGCCCCTCCATCCTGCGCCAGATCG
TGATTCCGCACGCGGCGCCCGGAATTCTGGCCGGCTGCATCGTGGTCTTCATGCTGACCCTGGGCAACTACCTCACGCCC
ACCTTGCTGGGCGGCAAAAACTCGCTATGGTTTACCGAGCAGATTTACACCCAGTTCATTACCCGTTTCAACTGGGAACA
GGGCGCTGCCTTCGGCTTTTTGCTGCTGGGGCTGTCCACCGCCATCGTCTGGCTGGGCCTGAAGCTCACGGGCCAGAAAT
TCGGTGAAGTGATGAGGAAGACATGA

Upstream 100 bases:

>100_bases
AAACCGGCCGTTTTGCCGACCTGCGCATCGGGGAAACCGTGGCCTTCAGCTTTGACCCGCAGCAGGCCGTCTGTTTCGCG
GCGGCGAGCGCCTGAGATTC

Downstream 100 bases:

>100_bases
CACACCCCCGTTGCGGACTCACTTCGTGTAGCGGCCTCCCCCCTGGCAGGGGGCGACACCTGCGGCCCGGCAAAGCCGGT
TCCGCGATGGACACGACACA

Product: binding-protein-dependent transport systems inner membrane component

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MQTAVAAPGIGTTPASTQARLILFLLLAPALIWLVGLIVLPHIDLAVLSLRERIAPRQYTPSIAQYRTFIEEPLYWHTFV
RTAVMSILATLITLLLAFPVAWTIAKLVRGRAKSLLLVLCLIPFWVSETVRTLGWMILLRESGVLPALLVQLGLTATPVE
MLYRDATILVGLVYTSMLFMVVPLISALESLDDALIEAAYDLGGTGPSILRQIVIPHAAPGILAGCIVVFMLTLGNYLTP
TLLGGKNSLWFTEQIYTQFITRFNWEQGAAFGFLLLGLSTAIVWLGLKLTGQKFGEVMRKT

Sequences:

>Translated_301_residues
MQTAVAAPGIGTTPASTQARLILFLLLAPALIWLVGLIVLPHIDLAVLSLRERIAPRQYTPSIAQYRTFIEEPLYWHTFV
RTAVMSILATLITLLLAFPVAWTIAKLVRGRAKSLLLVLCLIPFWVSETVRTLGWMILLRESGVLPALLVQLGLTATPVE
MLYRDATILVGLVYTSMLFMVVPLISALESLDDALIEAAYDLGGTGPSILRQIVIPHAAPGILAGCIVVFMLTLGNYLTP
TLLGGKNSLWFTEQIYTQFITRFNWEQGAAFGFLLLGLSTAIVWLGLKLTGQKFGEVMRKT
>Mature_301_residues
MQTAVAAPGIGTTPASTQARLILFLLLAPALIWLVGLIVLPHIDLAVLSLRERIAPRQYTPSIAQYRTFIEEPLYWHTFV
RTAVMSILATLITLLLAFPVAWTIAKLVRGRAKSLLLVLCLIPFWVSETVRTLGWMILLRESGVLPALLVQLGLTATPVE
MLYRDATILVGLVYTSMLFMVVPLISALESLDDALIEAAYDLGGTGPSILRQIVIPHAAPGILAGCIVVFMLTLGNYLTP
TLLGGKNSLWFTEQIYTQFITRFNWEQGAAFGFLLLGLSTAIVWLGLKLTGQKFGEVMRKT

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=266, Percent_Identity=33.4586466165414, Blast_Score=148, Evalue=4e-37,
Organism=Escherichia coli, GI1787080, Length=238, Percent_Identity=29.8319327731092, Blast_Score=130, Evalue=9e-32,
Organism=Escherichia coli, GI1787713, Length=212, Percent_Identity=32.0754716981132, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 33034; Mature: 33034

Theoretical pI: Translated: 9.34; Mature: 9.34

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTAVAAPGIGTTPASTQARLILFLLLAPALIWLVGLIVLPHIDLAVLSLRERIAPRQYT
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCC
PSIAQYRTFIEEPLYWHTFVRTAVMSILATLITLLLAFPVAWTIAKLVRGRAKSLLLVLC
CHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIPFWVSETVRTLGWMILLRESGVLPALLVQLGLTATPVEMLYRDATILVGLVYTSMLFM
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VVPLISALESLDDALIEAAYDLGGTGPSILRQIVIPHAAPGILAGCIVVFMLTLGNYLTP
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCH
TLLGGKNSLWFTEQIYTQFITRFNWEQGAAFGFLLLGLSTAIVWLGLKLTGQKFGEVMRK
HHCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
T
C
>Mature Secondary Structure
MQTAVAAPGIGTTPASTQARLILFLLLAPALIWLVGLIVLPHIDLAVLSLRERIAPRQYT
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCC
PSIAQYRTFIEEPLYWHTFVRTAVMSILATLITLLLAFPVAWTIAKLVRGRAKSLLLVLC
CHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIPFWVSETVRTLGWMILLRESGVLPALLVQLGLTATPVEMLYRDATILVGLVYTSMLFM
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VVPLISALESLDDALIEAAYDLGGTGPSILRQIVIPHAAPGILAGCIVVFMLTLGNYLTP
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCH
TLLGGKNSLWFTEQIYTQFITRFNWEQGAAFGFLLLGLSTAIVWLGLKLTGQKFGEVMRK
HHCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
T
C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]