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The map label for this gene is potD-A [H]

Identifier: 91789728

GI number: 91789728

Start: 4078858

End: 4079934

Strand: Direct

Name: potD-A [H]

Synonym: Bpro_3886

Alternate gene names: 91789728

Gene position: 4078858-4079934 (Clockwise)

Preceding gene: 91789716

Following gene: 91789729

Centisome position: 78.44

GC content: 59.24

Gene sequence:

>1077_bases
ATGAAATTATCTCGCCTGATGCGCCTCATGATGGGGGCCGGTCTGCTTGCTTCTAGCCTGGCCAATACGGCTTGGGCTCA
GGATCAGAAACTGCGGCTGATTACCTGGGCGGACTACGTGCCGGCTGATGTTGTGGCGCAGTTCAAAAAGGAAACAGGAA
TTGACGTCGAGCTCACCCTGTCGAACAACGAGGAGATGATCTCCAAATTGCGCGCCACCGGCGGTGCGGGTTTTGACCTG
GCCCAGCCCTCACAGGACCGCATCACCGGCCCGCAGCAGGAGTTTGGTATTTACAAACCCATGGACCTGAGCAAGATCAA
GTCCGAGCTGTTCATTCCTGCCATGCTCGACGCCACCAAAAAGAACACCACGCTGGCCGGCAAGGTCTATGGCCTGCCGC
ACATCTGGGGTACCGACGGCCTGGTGGTCAACACCCAGTTGGCGAAGATGAGCGACTACCCCGATTTGTGCAAAGCCGAT
GTCAAGGGAAAGACCGCCGTTCGCCTCAAGCGGCCCACCCTGCTGGCATTTGCCTTTGCCTCCGGCAAGGATCCGTTTGC
ACTTTACAAAGACCCAAAAGCCTATGGCGCGCTGATGGACGAAGTCGGCAAAACCATGATCGCCTGCAAGAGCAACATCA
AGTTTTACTGGGACAACAAGGACCAGCTGCTCAACGGCATGCGCACCGGCGAAGTGGTGGGCGCCATGATGTGGGATACC
GGCGGCTGGAAGCTCAACAGTGAAAAACCCGAAATCCAGTACATCGCCCCCAAGTCGGGCGCGCTCGGCTGGATAGACAC
CTTTGCCATCCCCGCCAAAGGCCGCAATGATGCTGCCGCGTATGCCTGGATCAACTTCAACATGCGTCCGGAAATCGCTG
CCAAGGTGGCGGGCTCCGCCGGCAACTTCACCGCCGCCAAAGGCGCTGACCAGCTGATGGACGCCAAGCTCAAGGCCCAG
TTCGCCGCCAGCTTCCCGGAAGCCGCCCTGAAGAACGTCAAATGGTATCCGGCCGTACCGGCCGGCATCGAAGACATCGA
GGGCCGTGTGCTGGACCGCATCAAGGCCGCCCGGTAA

Upstream 100 bases:

>100_bases
AACCAGTACAGGCTATGCTTTTTTCGCATGCTCATGCATTTCCGGGCTTTGCTGCAATCCCTTCGCGTCACATCACCATG
TCATCAACCAGGAAAATCCC

Downstream 100 bases:

>100_bases
CCATCGCCCGCCATTCAAGCGGTGCAGGCAGACCTTCAAGCCGATCACGTCGGTAAGCACTACGGCGGCCAGCTCGCCGT
CAAGGACTTGTCCCTGACGG

Product: extracellular solute-binding protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MKLSRLMRLMMGAGLLASSLANTAWAQDQKLRLITWADYVPADVVAQFKKETGIDVELTLSNNEEMISKLRATGGAGFDL
AQPSQDRITGPQQEFGIYKPMDLSKIKSELFIPAMLDATKKNTTLAGKVYGLPHIWGTDGLVVNTQLAKMSDYPDLCKAD
VKGKTAVRLKRPTLLAFAFASGKDPFALYKDPKAYGALMDEVGKTMIACKSNIKFYWDNKDQLLNGMRTGEVVGAMMWDT
GGWKLNSEKPEIQYIAPKSGALGWIDTFAIPAKGRNDAAAYAWINFNMRPEIAAKVAGSAGNFTAAKGADQLMDAKLKAQ
FAASFPEAALKNVKWYPAVPAGIEDIEGRVLDRIKAAR

Sequences:

>Translated_358_residues
MKLSRLMRLMMGAGLLASSLANTAWAQDQKLRLITWADYVPADVVAQFKKETGIDVELTLSNNEEMISKLRATGGAGFDL
AQPSQDRITGPQQEFGIYKPMDLSKIKSELFIPAMLDATKKNTTLAGKVYGLPHIWGTDGLVVNTQLAKMSDYPDLCKAD
VKGKTAVRLKRPTLLAFAFASGKDPFALYKDPKAYGALMDEVGKTMIACKSNIKFYWDNKDQLLNGMRTGEVVGAMMWDT
GGWKLNSEKPEIQYIAPKSGALGWIDTFAIPAKGRNDAAAYAWINFNMRPEIAAKVAGSAGNFTAAKGADQLMDAKLKAQ
FAASFPEAALKNVKWYPAVPAGIEDIEGRVLDRIKAAR
>Mature_358_residues
MKLSRLMRLMMGAGLLASSLANTAWAQDQKLRLITWADYVPADVVAQFKKETGIDVELTLSNNEEMISKLRATGGAGFDL
AQPSQDRITGPQQEFGIYKPMDLSKIKSELFIPAMLDATKKNTTLAGKVYGLPHIWGTDGLVVNTQLAKMSDYPDLCKAD
VKGKTAVRLKRPTLLAFAFASGKDPFALYKDPKAYGALMDEVGKTMIACKSNIKFYWDNKDQLLNGMRTGEVVGAMMWDT
GGWKLNSEKPEIQYIAPKSGALGWIDTFAIPAKGRNDAAAYAWINFNMRPEIAAKVAGSAGNFTAAKGADQLMDAKLKAQ
FAASFPEAALKNVKWYPAVPAGIEDIEGRVLDRIKAAR

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=326, Percent_Identity=25.7668711656442, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1787367, Length=338, Percent_Identity=29.2899408284024, Blast_Score=108, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 39100; Mature: 39100

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSRLMRLMMGAGLLASSLANTAWAQDQKLRLITWADYVPADVVAQFKKETGIDVELTL
CCHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEEE
SNNEEMISKLRATGGAGFDLAQPSQDRITGPQQEFGIYKPMDLSKIKSELFIPAMLDATK
CCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHCCHHHHHCCC
KNTTLAGKVYGLPHIWGTDGLVVNTQLAKMSDYPDLCKADVKGKTAVRLKRPTLLAFAFA
CCCEEEEEEECCCEEECCCCEEEEEHHHHCCCCHHHHHHCCCCCEEEEEECCEEEEEEEE
SGKDPFALYKDPKAYGALMDEVGKTMIACKSNIKFYWDNKDQLLNGMRTGEVVGAMMWDT
CCCCCEEEECCCHHHHHHHHHHHHEEEEECCCCEEEECCHHHHHCCCCCCHHEEEEEECC
GGWKLNSEKPEIQYIAPKSGALGWIDTFAIPAKGRNDAAAYAWINFNMRPEIAAKVAGSA
CCEEECCCCCCEEEECCCCCCCEEHHEEECCCCCCCCCEEEEEEECCCCHHHHHHCCCCC
GNFTAAKGADQLMDAKLKAQFAASFPEAALKNVKWYPAVPAGIEDIEGRVLDRIKAAR
CCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHCCEECCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKLSRLMRLMMGAGLLASSLANTAWAQDQKLRLITWADYVPADVVAQFKKETGIDVELTL
CCHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEEE
SNNEEMISKLRATGGAGFDLAQPSQDRITGPQQEFGIYKPMDLSKIKSELFIPAMLDATK
CCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHCCHHHHHCCC
KNTTLAGKVYGLPHIWGTDGLVVNTQLAKMSDYPDLCKADVKGKTAVRLKRPTLLAFAFA
CCCEEEEEEECCCEEECCCCEEEEEHHHHCCCCHHHHHHCCCCCEEEEEECCEEEEEEEE
SGKDPFALYKDPKAYGALMDEVGKTMIACKSNIKFYWDNKDQLLNGMRTGEVVGAMMWDT
CCCCCEEEECCCHHHHHHHHHHHHEEEEECCCCEEEECCHHHHHCCCCCCHHEEEEEECC
GGWKLNSEKPEIQYIAPKSGALGWIDTFAIPAKGRNDAAAYAWINFNMRPEIAAKVAGSA
CCEEECCCCCCEEEECCCCCCCEEHHEEECCCCCCCCCEEEEEEECCCCHHHHHHCCCCC
GNFTAAKGADQLMDAKLKAQFAASFPEAALKNVKWYPAVPAGIEDIEGRVLDRIKAAR
CCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHCCEECCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]