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The map label for this gene is garL [H]

Identifier: 91789699

GI number: 91789699

Start: 4048231

End: 4048992

Strand: Direct

Name: garL [H]

Synonym: Bpro_3855

Alternate gene names: 91789699

Gene position: 4048231-4048992 (Clockwise)

Preceding gene: 91789698

Following gene: 91789700

Centisome position: 77.85

GC content: 62.07

Gene sequence:

>762_bases
TTGACTGAGACCAGCTTCCGGCAACTGGTGCGTGCACGCAACCGGCTGGTGGGCACCTTTGTCAAGACGGCCAGCCATCA
GACGGTTGAGGTCCTCGGGACCACCGGGCTGGACTTCATTGTCATTGACGCTGAACACGCGCCCTTCAGTCAGAATGCGC
TGGACACCTGCCTGCTCGCTGCCAGAGCCACGCAGCTGCCCGCGCTGGTCAGGATTGCCGATACCGAGCCGGCCACGATA
CTGCAGGCACTGGACATGGGCGCTACCGGATTGCTGGTACCCCACGCCAAGACGGTTGCGGGCATCCATGCGGTGCTGGC
ATGCACTCAGTACCGCCGTGGCAGCAGGGGATTTTCCAACTCATCCCGAGCCGGACAGTACGGCACGACTCCCATGCTGC
AGCTGGTGGACTCCTCCGACCAGGACGTAGCGGTAGTGTTCCAGATCGAGGACCGTGAGGCCGTTGAAGCGATAGAGACC
TTGGCCGCCATGGAAGAAGTGGATGGCTTCCTGATCGGGCGTGCCGACCTAGCCATGTCCTATGGCGTTTTTGACGCGGC
ACATCCCGAGGTGTACGCGGCTGCTGTTCGGGTCTGCAAGGCTTGTGCGGCGGCTAACAAGCCGGTTGGCATGTTCCTGG
CCGACGCGAGCGAACTGGAGCACTGGGTTCAATTGGGTGTCAGCCTGTTTGTGATGGGATCCGATCAGGCCTTGCTGCGT
GCACAGGCCAACGCCATGGCCAGCAAAGTCCGGTCTGCCTGA

Upstream 100 bases:

>100_bases
GGCTATGAGCCCATCGGGGACACGCCGCAACAGTTTGAAGCCTTCCTGAAAAAGGACAAAGACGTTTCCGGCAAGCGCAT
CAAGGCACTGGGGATCAAAC

Downstream 100 bases:

>100_bases
TGGCCGGAATCATTTTTTCATATTCGTATTGATAAGGAACACCCCATGACTCAAATGTTTGACTGGAACAAGCTGCCCCG
CGAATTTGTGCGCGAAGGCA

Product: HpcH/HpaI aldolase

Products: NA

Alternate protein names: KDGluc aldolase; KDGlucA; 2-dehydro-3-deoxy-D-glucarate aldolase; 2-keto-3-deoxy-D-glucarate aldolase; 5-dehydro-4-deoxy-D-glucarate aldolase; Alpha-keto-beta-deoxy-D-glucarate aldolase [H]

Number of amino acids: Translated: 253; Mature: 252

Protein sequence:

>253_residues
MTETSFRQLVRARNRLVGTFVKTASHQTVEVLGTTGLDFIVIDAEHAPFSQNALDTCLLAARATQLPALVRIADTEPATI
LQALDMGATGLLVPHAKTVAGIHAVLACTQYRRGSRGFSNSSRAGQYGTTPMLQLVDSSDQDVAVVFQIEDREAVEAIET
LAAMEEVDGFLIGRADLAMSYGVFDAAHPEVYAAAVRVCKACAAANKPVGMFLADASELEHWVQLGVSLFVMGSDQALLR
AQANAMASKVRSA

Sequences:

>Translated_253_residues
MTETSFRQLVRARNRLVGTFVKTASHQTVEVLGTTGLDFIVIDAEHAPFSQNALDTCLLAARATQLPALVRIADTEPATI
LQALDMGATGLLVPHAKTVAGIHAVLACTQYRRGSRGFSNSSRAGQYGTTPMLQLVDSSDQDVAVVFQIEDREAVEAIET
LAAMEEVDGFLIGRADLAMSYGVFDAAHPEVYAAAVRVCKACAAANKPVGMFLADASELEHWVQLGVSLFVMGSDQALLR
AQANAMASKVRSA
>Mature_252_residues
TETSFRQLVRARNRLVGTFVKTASHQTVEVLGTTGLDFIVIDAEHAPFSQNALDTCLLAARATQLPALVRIADTEPATIL
QALDMGATGLLVPHAKTVAGIHAVLACTQYRRGSRGFSNSSRAGQYGTTPMLQLVDSSDQDVAVVFQIEDREAVEAIETL
AAMEEVDGFLIGRADLAMSYGVFDAAHPEVYAAAVRVCKACAAANKPVGMFLADASELEHWVQLGVSLFVMGSDQALLRA
QANAMASKVRSA

Specific function: Catalyzes the reversible retro-aldol cleavage of both 5- keto-4-deoxy-D-glucarate and 2-keto-3-deoxy-D-glucarate to pyruvate and tartronic semialdehyde [H]

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family. KDGluc aldolase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789514, Length=250, Percent_Identity=28.4, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1788578, Length=244, Percent_Identity=29.5081967213115, Blast_Score=92, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR017648
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: =4.1.2.20 [H]

Molecular weight: Translated: 26939; Mature: 26808

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTETSFRQLVRARNRLVGTFVKTASHQTVEVLGTTGLDFIVIDAEHAPFSQNALDTCLLA
CCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHH
ARATQLPALVRIADTEPATILQALDMGATGLLVPHAKTVAGIHAVLACTQYRRGSRGFSN
HHHHCCCEEEEECCCCHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCCCC
SSRAGQYGTTPMLQLVDSSDQDVAVVFQIEDREAVEAIETLAAMEEVDGFLIGRADLAMS
CCCCCCCCCCHHHHEECCCCCCEEEEEEECCHHHHHHHHHHHHHHHHCCEEEEHHHHHHH
YGVFDAAHPEVYAAAVRVCKACAAANKPVGMFLADASELEHWVQLGVSLFVMGSDQALLR
HCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHCEEEEEECCCHHHHH
AQANAMASKVRSA
HHHHHHHHHHHCC
>Mature Secondary Structure 
TETSFRQLVRARNRLVGTFVKTASHQTVEVLGTTGLDFIVIDAEHAPFSQNALDTCLLA
CCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHH
ARATQLPALVRIADTEPATILQALDMGATGLLVPHAKTVAGIHAVLACTQYRRGSRGFSN
HHHHCCCEEEEECCCCHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCCCC
SSRAGQYGTTPMLQLVDSSDQDVAVVFQIEDREAVEAIETLAAMEEVDGFLIGRADLAMS
CCCCCCCCCCHHHHEECCCCCCEEEEEEECCHHHHHHHHHHHHHHHHCCEEEEHHHHHHH
YGVFDAAHPEVYAAAVRVCKACAAANKPVGMFLADASELEHWVQLGVSLFVMGSDQALLR
HCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHCEEEEEECCCHHHHH
AQANAMASKVRSA
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA