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The map label for this gene is mhpA [H]

Identifier: 91789564

GI number: 91789564

Start: 3924080

End: 3925837

Strand: Direct

Name: mhpA [H]

Synonym: Bpro_3718

Alternate gene names: 91789564

Gene position: 3924080-3925837 (Clockwise)

Preceding gene: 91789563

Following gene: 91789565

Centisome position: 75.46

GC content: 64.96

Gene sequence:

>1758_bases
ATGCAAGAAGAACATCCGGACTACGCTGAAGCCGCCTTCACCAACGGCTATGAATTCACGCAGGGCACGGGCTACCACCT
GCCCGAATACCCATTTATCGCACCGCCCGAACTCACCTCGGGCGAGGTCAGGCGTCACCCGATTGTCATTGTGGGCGGTG
GCATCACCGGCCTGACGCTGACCTGCCTGCTGGCGCATTACGGTGTGCCGGTGATACTGCTCGACGAAGACAACACGGTG
GGCGTCAAGGGGGCTTCGTCCCGCGGCATTTGCTACGCGCAAAAGACACTCGAAATCTTCAAACGCCTGGGCATTTACGG
GCGCATTGCCGCCAGGGGCGTGCAGTGGAGCGTGGGACGCACCTTTGCCGGCGACGACGAGGTCTACAGCTTCGACCTGC
GCCAGCAGCAAACCCTCTCCCTGAGCAAAGAACCTCCCTTCATCAACATCCAGCAGTTCTATGTGGAAGGTTTTCTGGTC
GAACGGCTCTACCAGCTGGCCCAGGCCCATCCGGGCGTGGAGCTCCGCTGGCGCCACCGGGTTACCGGGCTTGAGCAGAA
TGCCGAAACCGCGACGCTATCCATCGACACGCCGGCCGGGACCTATTCGCTGCAGGCCGACCATGTGGTTGACTGCAGCG
GCTCGCACAGCCCGTTTCGCGGCTGGTGCCAGGCCACGGTCGCCACCAAAAAGGGCGACGACCGCTGGTGCATTGCCGAC
GTGCGTTTCAGCAACCCGCTACCGGTGGAGCGCCACACCTGGATAGAGGCGCCATTCAACGAAGGCCGTGCCGTCTGGCA
GCACCTGATGGCCGACGGCGTCTGGCGCATTGACTACCAGATGCCGCCCGACGCCGACCTGCAATCAATGAGCCGCGAAG
ACGTCGTGCGGGCCCGGCTGAACGCCCAATTCAAGCGTGAGCTGAGTCCGGAGGAATGTGAAATCGTCTGGGTGGGCCCT
TACGCCTACCGCAGCGAATGCCTGGACATGCTGCGCCACGGCCGGGTGTTTTTTGCCGGCGATGCTGCCCATGTGGTCAG
CCCCTTTGGTGCGCGCGGCGGCAACTCGGGGGTGCAGGACGCCGACAACCTGGCCTGGAAGCTGGCCGCCGTGGTGGAGG
ACCGCGCCGCGCCCCAGTTGCTGGACAGCTACAGCGACGAGCGGCGTGAGGCCGCACAGTTCAACATGCGGGTCACCAAC
CGCACCACACGCTTTTTGCGCCCCGCCGATGGCGCGGAACGCCTGTTTCGCAATGCCGTCCTGAGCCTGGCCAGGCAGCA
TGCGTTTGCCCGGCAACTGGTCAACACCGGCCGCATGTCCACCCCCAACACCTACACCCACTCCAGCGTGTGCGGCAACA
CCGGTGGCCTGGCCACGCAAAACGCCGCCCTCACCTGGCCCAACGGGACCCGCGGCGAGCTGATTGACCTGCTCAACTGG
GCCGGCAGCCGGCTGCTGCTGCTGGTGTTTGGCGACCTGCCCGCCACCGCCGCCAGGCGGCTCCAGGAACTGGCCGTACA
AACCGACGTACGCTGCGTGCAGGTGATTCCCCCCGACAGCCGGGCCAGCGCCGTGGAGCACGTGATTGACCAGGAGGGTC
ATCTGCAGGCCAGCTGCCACGTCTTTGGCCATGCCTGGGCACTGCTGCGGCCCGACAGCTACGTGGCCGCGACCGGCGAA
AGCATTGACACCTCACTGGTGCATGCCATTGCCATGGCGCTTGGCACCCACTGGAGCCTGCACGGAGAAACCGCATGA

Upstream 100 bases:

>100_bases
GCCCGAAGTTGCCGCCAGAGCGATGGCGCGGCCATAGAAGGCGGCCAGGGATTGCAGCAGGAAATCGTGTTGGTAAATGC
AGGCAGGAGGGACATCACCC

Downstream 100 bases:

>100_bases
AAACCAACGTCAACTTCCAGGATGCCGATGCGTTTTACGAATCCCTGCTCGACGCCCACCAGGGCCTGAGCCGGGAGCAG
TCCGAGTTGCTCAATGCCCG

Product: FAD-binding monooxygenase

Products: H2O; 3-(2,3-dihydroxyphenyl)propionate; NAD [C]

Alternate protein names: 3-HCI hydroxylase; 3-HPP hydroxylase [H]

Number of amino acids: Translated: 585; Mature: 585

Protein sequence:

>585_residues
MQEEHPDYAEAAFTNGYEFTQGTGYHLPEYPFIAPPELTSGEVRRHPIVIVGGGITGLTLTCLLAHYGVPVILLDEDNTV
GVKGASSRGICYAQKTLEIFKRLGIYGRIAARGVQWSVGRTFAGDDEVYSFDLRQQQTLSLSKEPPFINIQQFYVEGFLV
ERLYQLAQAHPGVELRWRHRVTGLEQNAETATLSIDTPAGTYSLQADHVVDCSGSHSPFRGWCQATVATKKGDDRWCIAD
VRFSNPLPVERHTWIEAPFNEGRAVWQHLMADGVWRIDYQMPPDADLQSMSREDVVRARLNAQFKRELSPEECEIVWVGP
YAYRSECLDMLRHGRVFFAGDAAHVVSPFGARGGNSGVQDADNLAWKLAAVVEDRAAPQLLDSYSDERREAAQFNMRVTN
RTTRFLRPADGAERLFRNAVLSLARQHAFARQLVNTGRMSTPNTYTHSSVCGNTGGLATQNAALTWPNGTRGELIDLLNW
AGSRLLLLVFGDLPATAARRLQELAVQTDVRCVQVIPPDSRASAVEHVIDQEGHLQASCHVFGHAWALLRPDSYVAATGE
SIDTSLVHAIAMALGTHWSLHGETA

Sequences:

>Translated_585_residues
MQEEHPDYAEAAFTNGYEFTQGTGYHLPEYPFIAPPELTSGEVRRHPIVIVGGGITGLTLTCLLAHYGVPVILLDEDNTV
GVKGASSRGICYAQKTLEIFKRLGIYGRIAARGVQWSVGRTFAGDDEVYSFDLRQQQTLSLSKEPPFINIQQFYVEGFLV
ERLYQLAQAHPGVELRWRHRVTGLEQNAETATLSIDTPAGTYSLQADHVVDCSGSHSPFRGWCQATVATKKGDDRWCIAD
VRFSNPLPVERHTWIEAPFNEGRAVWQHLMADGVWRIDYQMPPDADLQSMSREDVVRARLNAQFKRELSPEECEIVWVGP
YAYRSECLDMLRHGRVFFAGDAAHVVSPFGARGGNSGVQDADNLAWKLAAVVEDRAAPQLLDSYSDERREAAQFNMRVTN
RTTRFLRPADGAERLFRNAVLSLARQHAFARQLVNTGRMSTPNTYTHSSVCGNTGGLATQNAALTWPNGTRGELIDLLNW
AGSRLLLLVFGDLPATAARRLQELAVQTDVRCVQVIPPDSRASAVEHVIDQEGHLQASCHVFGHAWALLRPDSYVAATGE
SIDTSLVHAIAMALGTHWSLHGETA
>Mature_585_residues
MQEEHPDYAEAAFTNGYEFTQGTGYHLPEYPFIAPPELTSGEVRRHPIVIVGGGITGLTLTCLLAHYGVPVILLDEDNTV
GVKGASSRGICYAQKTLEIFKRLGIYGRIAARGVQWSVGRTFAGDDEVYSFDLRQQQTLSLSKEPPFINIQQFYVEGFLV
ERLYQLAQAHPGVELRWRHRVTGLEQNAETATLSIDTPAGTYSLQADHVVDCSGSHSPFRGWCQATVATKKGDDRWCIAD
VRFSNPLPVERHTWIEAPFNEGRAVWQHLMADGVWRIDYQMPPDADLQSMSREDVVRARLNAQFKRELSPEECEIVWVGP
YAYRSECLDMLRHGRVFFAGDAAHVVSPFGARGGNSGVQDADNLAWKLAAVVEDRAAPQLLDSYSDERREAAQFNMRVTN
RTTRFLRPADGAERLFRNAVLSLARQHAFARQLVNTGRMSTPNTYTHSSVCGNTGGLATQNAALTWPNGTRGELIDLLNW
AGSRLLLLVFGDLPATAARRLQELAVQTDVRCVQVIPPDSRASAVEHVIDQEGHLQASCHVFGHAWALLRPDSYVAATGE
SIDTSLVHAIAMALGTHWSLHGETA

Specific function: Catalyzes the insertion of one atom of molecular oxygen into position 2 of the phenyl ring of 3-(3- hydroxyphenyl)propionate (3-HPP) and hydroxycinnamic acid (3HCI) [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786543, Length=525, Percent_Identity=21.7142857142857, Blast_Score=100, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.- [C]

Molecular weight: Translated: 64777; Mature: 64777

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQEEHPDYAEAAFTNGYEFTQGTGYHLPEYPFIAPPELTSGEVRRHPIVIVGGGITGLTL
CCCCCCCHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHH
TCLLAHYGVPVILLDEDNTVGVKGASSRGICYAQKTLEIFKRLGIYGRIAARGVQWSVGR
HHHHHHCCCCEEEECCCCEEEECCCCCCCEEEHHHHHHHHHHCCCCEEHHHCCEEEECCC
TFAGDDEVYSFDLRQQQTLSLSKEPPFINIQQFYVEGFLVERLYQLAQAHPGVELRWRHR
EECCCCCEEEECCCHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEC
VTGLEQNAETATLSIDTPAGTYSLQADHVVDCSGSHSPFRGWCQATVATKKGDDRWCIAD
CCCCCCCCCEEEEEEECCCCEEEECCCCEEECCCCCCCCCHHHEEEEEECCCCCCEEEEE
VRFSNPLPVERHTWIEAPFNEGRAVWQHLMADGVWRIDYQMPPDADLQSMSREDVVRARL
EECCCCCCCCCCEEEECCCCCCHHHHHHHHHCCEEEEEECCCCCCCHHHCCHHHHHHHHH
NAQFKRELSPEECEIVWVGPYAYRSECLDMLRHGRVFFAGDAAHVVSPFGARGGNSGVQD
HHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHCCCEEEECCHHHHHCCCCCCCCCCCCCC
ADNLAWKLAAVVEDRAAPQLLDSYSDERREAAQFNMRVTNRTTRFLRPADGAERLFRNAV
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEEEEECCHHHEECCCCHHHHHHHHHH
LSLARQHAFARQLVNTGRMSTPNTYTHSSVCGNTGGLATQNAALTWPNGTRGELIDLLNW
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHC
AGSRLLLLVFGDLPATAARRLQELAVQTDVRCVQVIPPDSRASAVEHVIDQEGHLQASCH
CCCEEEEEEECCCCHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHCCCCCEEEEEE
VFGHAWALLRPDSYVAATGESIDTSLVHAIAMALGTHWSLHGETA
EEEEEEEEECCCCEEEECCCCHHHHHHHHHHHHHCCCEEECCCCC
>Mature Secondary Structure
MQEEHPDYAEAAFTNGYEFTQGTGYHLPEYPFIAPPELTSGEVRRHPIVIVGGGITGLTL
CCCCCCCHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHH
TCLLAHYGVPVILLDEDNTVGVKGASSRGICYAQKTLEIFKRLGIYGRIAARGVQWSVGR
HHHHHHCCCCEEEECCCCEEEECCCCCCCEEEHHHHHHHHHHCCCCEEHHHCCEEEECCC
TFAGDDEVYSFDLRQQQTLSLSKEPPFINIQQFYVEGFLVERLYQLAQAHPGVELRWRHR
EECCCCCEEEECCCHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEC
VTGLEQNAETATLSIDTPAGTYSLQADHVVDCSGSHSPFRGWCQATVATKKGDDRWCIAD
CCCCCCCCCEEEEEEECCCCEEEECCCCEEECCCCCCCCCHHHEEEEEECCCCCCEEEEE
VRFSNPLPVERHTWIEAPFNEGRAVWQHLMADGVWRIDYQMPPDADLQSMSREDVVRARL
EECCCCCCCCCCEEEECCCCCCHHHHHHHHHCCEEEEEECCCCCCCHHHCCHHHHHHHHH
NAQFKRELSPEECEIVWVGPYAYRSECLDMLRHGRVFFAGDAAHVVSPFGARGGNSGVQD
HHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHCCCEEEECCHHHHHCCCCCCCCCCCCCC
ADNLAWKLAAVVEDRAAPQLLDSYSDERREAAQFNMRVTNRTTRFLRPADGAERLFRNAV
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEEEEECCHHHEECCCCHHHHHHHHHH
LSLARQHAFARQLVNTGRMSTPNTYTHSSVCGNTGGLATQNAALTWPNGTRGELIDLLNW
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHC
AGSRLLLLVFGDLPATAARRLQELAVQTDVRCVQVIPPDSRASAVEHVIDQEGHLQASCH
CCCEEEEEEECCCCHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHCCCCCEEEEEE
VFGHAWALLRPDSYVAATGESIDTSLVHAIAMALGTHWSLHGETA
EEEEEEEEECCCCEEEECCCCHHHHHHHHHHHHHCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-(3-hydroxyphenyl)propionate; NADH; O2 [C]

Specific reaction: 3-(3-hydroxyphenyl)propionate + NADH + O2 = H2O + 3-(2,3-dihydroxyphenyl)propionate + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA