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The map label for this gene is queF

Identifier: 91789556

GI number: 91789556

Start: 3916504

End: 3917331

Strand: Reverse

Name: queF

Synonym: Bpro_3710

Alternate gene names: 91789556

Gene position: 3917331-3916504 (Counterclockwise)

Preceding gene: 91789557

Following gene: 91789555

Centisome position: 75.33

GC content: 60.39

Gene sequence:

>828_bases
ATGAATACCCCTGAACACTCCCAACTCGGTAAGTCCTCGGCCTATGTTGACCAGTACGATGCCTCCTTGCTCTTTCCGAT
CCCCCGTGCGGAAAAACGTGCCGAAATCGGCGTCACCGGAACACCGCCGTTTTTTGGCGCCGACATGTGGACCGCATTCG
AGCTGAGCTGGCTCAATATGCGCGGCAAGCCGCAGGTGGCTTTGGCGCACATCACCGTGCCCTGCGAGTCGCCCAACATT
GTCGAGAGCAAGTCCTTCAAGCTTTACCTGAACAGCTTCAATAACACGCGTTTTTCCGACGCGCGCGATGTGCGCGAGCG
CATCCGCGCCGACATCAATGCAGCGGTGGGCGCTGGCGTGGGGGTTAAAACACTGGGGCCGGAACTGTTTGACCGCGAAC
CCGTGCATGAGCTCGATGGCCTGAGCCTGGACCGGCTCGATGTGGAGTGCATCCACTTCACGCCGGCGCCGGAACTGTTA
TTTGCAGAGTTCGACGAGCCGCCGGTCGATGAAACCCTGACCAGCAACCTGCTCAAAAGCAACTGCCTGGTGACCGGGCA
GCCCGATTGGGGCAGTGTGCAGATCAGCTACTCGGGGCCACAAATCAACCAGGAAGGCCTGCTGCAATACCTGGTGAGCT
TTCGCAACCACAACGAATTTCACGAGCAGTGCGTGGAGCGCATCTTCATGGACGTGTGGACGCGCTGCAGGCCGCTGAAG
CTGTCGGTGTACGCCCGCTACACCCGCCGCGGAGGGCTGGACATCAACCCGTTTCGCACCAGCCATCCGCAAGCCCTGCC
GGCCAATATCCGGATGGCGCGGCAGTAA

Upstream 100 bases:

>100_bases
CTGTCCCTTTCTCTACGTAAAGGGGTCTTCGCTGGCCCGGTGCGGCCCTTCGCGGCGCGGTCAGCTACATTGATCAAGAT
GATCAGAGAATTTACGACCC

Downstream 100 bases:

>100_bases
GTTGCATTTGTGTATTGATTTGCACATGGGTTGGCCAACCTCGCAGTGCCGGGCATGGCCCGCGTGACTTCGCGCGTGTT
GCGGTGCAGTAGACCAGGCC

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MNTPEHSQLGKSSAYVDQYDASLLFPIPRAEKRAEIGVTGTPPFFGADMWTAFELSWLNMRGKPQVALAHITVPCESPNI
VESKSFKLYLNSFNNTRFSDARDVRERIRADINAAVGAGVGVKTLGPELFDREPVHELDGLSLDRLDVECIHFTPAPELL
FAEFDEPPVDETLTSNLLKSNCLVTGQPDWGSVQISYSGPQINQEGLLQYLVSFRNHNEFHEQCVERIFMDVWTRCRPLK
LSVYARYTRRGGLDINPFRTSHPQALPANIRMARQ

Sequences:

>Translated_275_residues
MNTPEHSQLGKSSAYVDQYDASLLFPIPRAEKRAEIGVTGTPPFFGADMWTAFELSWLNMRGKPQVALAHITVPCESPNI
VESKSFKLYLNSFNNTRFSDARDVRERIRADINAAVGAGVGVKTLGPELFDREPVHELDGLSLDRLDVECIHFTPAPELL
FAEFDEPPVDETLTSNLLKSNCLVTGQPDWGSVQISYSGPQINQEGLLQYLVSFRNHNEFHEQCVERIFMDVWTRCRPLK
LSVYARYTRRGGLDINPFRTSHPQALPANIRMARQ
>Mature_275_residues
MNTPEHSQLGKSSAYVDQYDASLLFPIPRAEKRAEIGVTGTPPFFGADMWTAFELSWLNMRGKPQVALAHITVPCESPNI
VESKSFKLYLNSFNNTRFSDARDVRERIRADINAAVGAGVGVKTLGPELFDREPVHELDGLSLDRLDVECIHFTPAPELL
FAEFDEPPVDETLTSNLLKSNCLVTGQPDWGSVQISYSGPQINQEGLLQYLVSFRNHNEFHEQCVERIFMDVWTRCRPLK
LSVYARYTRRGGLDINPFRTSHPQALPANIRMARQ

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1)

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily

Homologues:

Organism=Escherichia coli, GI1789158, Length=269, Percent_Identity=50.9293680297398, Blast_Score=272, Evalue=2e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEF_POLSJ (Q126D2)

Other databases:

- EMBL:   CP000316
- RefSeq:   YP_550508.1
- ProteinModelPortal:   Q126D2
- SMR:   Q126D2
- STRING:   Q126D2
- GeneID:   4013621
- GenomeReviews:   CP000316_GR
- KEGG:   pol:Bpro_3710
- NMPDR:   fig|296591.1.peg.1628
- eggNOG:   COG0780
- HOGENOM:   HBG289139
- OMA:   NPFRTSH
- PhylomeDB:   Q126D2
- ProtClustDB:   PRK11792
- BioCyc:   PSP296591:BPRO_3710-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00817
- InterPro:   IPR016428
- InterPro:   IPR020602
- PIRSF:   PIRSF004750
- TIGRFAMs:   TIGR03138

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =1.7.1.13

Molecular weight: Translated: 31053; Mature: 31053

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTPEHSQLGKSSAYVDQYDASLLFPIPRAEKRAEIGVTGTPPFFGADMWTAFELSWLNM
CCCCCCHHCCCCCCHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCCCCHHHEEEEEEECC
RGKPQVALAHITVPCESPNIVESKSFKLYLNSFNNTRFSDARDVRERIRADINAAVGAGV
CCCCCEEEEEEEEECCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCC
GVKTLGPELFDREPVHELDGLSLDRLDVECIHFTPAPELLFAEFDEPPVDETLTSNLLKS
CHHHCCHHHCCCCCHHHHCCCCCCCEEEEEEEECCCCCCEEECCCCCCCHHHHHHHHHHC
NCLVTGQPDWGSVQISYSGPQINQEGLLQYLVSFRNHNEFHEQCVERIFMDVWTRCRPLK
CCEEECCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEE
LSVYARYTRRGGLDINPFRTSHPQALPANIRMARQ
EEEEEEEHHCCCCCCCCCCCCCCCCCCCCCEECCC
>Mature Secondary Structure
MNTPEHSQLGKSSAYVDQYDASLLFPIPRAEKRAEIGVTGTPPFFGADMWTAFELSWLNM
CCCCCCHHCCCCCCHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCCCCHHHEEEEEEECC
RGKPQVALAHITVPCESPNIVESKSFKLYLNSFNNTRFSDARDVRERIRADINAAVGAGV
CCCCCEEEEEEEEECCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCC
GVKTLGPELFDREPVHELDGLSLDRLDVECIHFTPAPELLFAEFDEPPVDETLTSNLLKS
CHHHCCHHHCCCCCHHHHCCCCCCCEEEEEEEECCCCCCEEECCCCCCCHHHHHHHHHHC
NCLVTGQPDWGSVQISYSGPQINQEGLLQYLVSFRNHNEFHEQCVERIFMDVWTRCRPLK
CCEEECCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEE
LSVYARYTRRGGLDINPFRTSHPQALPANIRMARQ
EEEEEEEHHCCCCCCCCCCCCCCCCCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA