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The map label for this gene is est [H]

Identifier: 91789285

GI number: 91789285

Start: 3631325

End: 3632218

Strand: Reverse

Name: est [H]

Synonym: Bpro_3430

Alternate gene names: 91789285

Gene position: 3632218-3631325 (Counterclockwise)

Preceding gene: 91789287

Following gene: 91789284

Centisome position: 69.85

GC content: 69.46

Gene sequence:

>894_bases
ATGCGGCCACAGATGATGAACAGCCAGCAGCTCACCCGCTCAGGCACCGACAAGGCCGCACCCGCCTGGGTGGACAAGCA
GATCCAGCTTTCGCTGAAAAAGCCGCTGGATGTGCGCTTTTACGGCCGCCACCGTGTCGGCGCCGTGTCGCCGCTGGTGG
TGCACTTTCACGGCGGCGCGTTTGCCGGTGGTTCGCTGGACGGCGGCTCCTGTGTGGCCCGGCTGCTGGCCGACGCCGGC
GCCGTGGTCATGTCGCTCGACTATCCGCTGGCGCCTGCGCATCCTTTTCCGGCCGCCGTTGAAACCGGTTATGCCGCGCT
GCTGTGGGCCTGGAAAGCGCGCCAGAAACTGGCCGGGCAGGGCGCACCTCTTTTCATTGCAGGTGAGGAAGCCGGTGGCA
ACCTGGCGGCCGCGGTCTCGCTGGTCGCGCGTGACCGGCAGGAGCCCGCGCTGGCCGGGCAGATCCTGCTGTCACCCATG
CTGGATCCCTGCCTGGCGACGGCCTCGCTGCGCGATGCCGAAGCCGGCCCCGTCGGTTGTGTCTGGGCCGACGGCTGGCA
CCACTACCTGCCCCGCCTGGAAGACGCCAGCCATCCCTATGCCGCGCCGGGCTCGGCCCTGCGCCTGACCGGGCTGCCAC
CGACCTTGCTGGTGACCGCCCAGGATGATCCGCTGCGCGACGAAACGCTGGAGTATGCCGAACGGCTGCGCGCTGCCGGG
CTGGTTGCGCAGCACGCCGTGCTGCCGGTGGTGACTGGCTGGCCCTGCAGTTACCTCCAGCCTGCCAGCCACGAGGCGGC
GTGGGCGCCGGCGGTGCGGCAGCTGTTCACCGAATTTTTCGCCGGCACCCCTGCGCTGCAGGCCGGGCACACCCATTCGT
CTTCCGTTTCCTGA

Upstream 100 bases:

>100_bases
TTGATTATTTCACAGGAGAAATAATGAATGAGCGAAGTGGCTATTTATTGATTGAGGCGGTGGGCTTATCTTCTGCTGCA
TTGCAACAACCGAGGGCCGT

Downstream 100 bases:

>100_bases
ATCCGTTTTCTTTCTTTGTTTTTTTTGAGGGAGTTCACCACCATGCCATCGATTCAATTACCGGCCAACAAGCGCCGACT
TCTGGCCGTTGCTCTGGCCG

Product: alpha/beta hydrolase fold-3 protein

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MRPQMMNSQQLTRSGTDKAAPAWVDKQIQLSLKKPLDVRFYGRHRVGAVSPLVVHFHGGAFAGGSLDGGSCVARLLADAG
AVVMSLDYPLAPAHPFPAAVETGYAALLWAWKARQKLAGQGAPLFIAGEEAGGNLAAAVSLVARDRQEPALAGQILLSPM
LDPCLATASLRDAEAGPVGCVWADGWHHYLPRLEDASHPYAAPGSALRLTGLPPTLLVTAQDDPLRDETLEYAERLRAAG
LVAQHAVLPVVTGWPCSYLQPASHEAAWAPAVRQLFTEFFAGTPALQAGHTHSSSVS

Sequences:

>Translated_297_residues
MRPQMMNSQQLTRSGTDKAAPAWVDKQIQLSLKKPLDVRFYGRHRVGAVSPLVVHFHGGAFAGGSLDGGSCVARLLADAG
AVVMSLDYPLAPAHPFPAAVETGYAALLWAWKARQKLAGQGAPLFIAGEEAGGNLAAAVSLVARDRQEPALAGQILLSPM
LDPCLATASLRDAEAGPVGCVWADGWHHYLPRLEDASHPYAAPGSALRLTGLPPTLLVTAQDDPLRDETLEYAERLRAAG
LVAQHAVLPVVTGWPCSYLQPASHEAAWAPAVRQLFTEFFAGTPALQAGHTHSSSVS
>Mature_297_residues
MRPQMMNSQQLTRSGTDKAAPAWVDKQIQLSLKKPLDVRFYGRHRVGAVSPLVVHFHGGAFAGGSLDGGSCVARLLADAG
AVVMSLDYPLAPAHPFPAAVETGYAALLWAWKARQKLAGQGAPLFIAGEEAGGNLAAAVSLVARDRQEPALAGQILLSPM
LDPCLATASLRDAEAGPVGCVWADGWHHYLPRLEDASHPYAAPGSALRLTGLPPTLLVTAQDDPLRDETLEYAERLRAAG
LVAQHAVLPVVTGWPCSYLQPASHEAAWAPAVRQLFTEFFAGTPALQAGHTHSSSVS

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Secreted (Potential). Note=External to the cytoplasmic membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI21328446, Length=93, Percent_Identity=41.9354838709677, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 31368; Mature: 31368

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPQMMNSQQLTRSGTDKAAPAWVDKQIQLSLKKPLDVRFYGRHRVGAVSPLVVHFHGGA
CCCCCCCCHHHHHCCCCCCCCCCCCCEEEEEECCCCCEEEECCCCCCCCCEEEEEECCCE
FAGGSLDGGSCVARLLADAGAVVMSLDYPLAPAHPFPAAVETGYAALLWAWKARQKLAGQ
ECCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHCCC
GAPLFIAGEEAGGNLAAAVSLVARDRQEPALAGQILLSPMLDPCLATASLRDAEAGPVGC
CCCEEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
VWADGWHHYLPRLEDASHPYAAPGSALRLTGLPPTLLVTAQDDPLRDETLEYAERLRAAG
EEECCHHHHCCCCCCCCCCCCCCCCEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHH
LVAQHAVLPVVTGWPCSYLQPASHEAAWAPAVRQLFTEFFAGTPALQAGHTHSSSVS
HHHHHHHHEEECCCCHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRPQMMNSQQLTRSGTDKAAPAWVDKQIQLSLKKPLDVRFYGRHRVGAVSPLVVHFHGGA
CCCCCCCCHHHHHCCCCCCCCCCCCCEEEEEECCCCCEEEECCCCCCCCCEEEEEECCCE
FAGGSLDGGSCVARLLADAGAVVMSLDYPLAPAHPFPAAVETGYAALLWAWKARQKLAGQ
ECCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHCCC
GAPLFIAGEEAGGNLAAAVSLVARDRQEPALAGQILLSPMLDPCLATASLRDAEAGPVGC
CCCEEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
VWADGWHHYLPRLEDASHPYAAPGSALRLTGLPPTLLVTAQDDPLRDETLEYAERLRAAG
EEECCHHHHCCCCCCCCCCCCCCCCEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHH
LVAQHAVLPVVTGWPCSYLQPASHEAAWAPAVRQLFTEFFAGTPALQAGHTHSSSVS
HHHHHHHHEEECCCCHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2545531 [H]