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The map label for this gene is oprM [H]

Identifier: 91789282

GI number: 91789282

Start: 3625332

End: 3626744

Strand: Reverse

Name: oprM [H]

Synonym: Bpro_3427

Alternate gene names: 91789282

Gene position: 3626744-3625332 (Counterclockwise)

Preceding gene: 91789283

Following gene: 91789281

Centisome position: 69.74

GC content: 68.79

Gene sequence:

>1413_bases
ATGAACCGCATTCATTCAACGCTGCGCATGTCGCTGGCGCCCCTGGTGGCAGCGCTGGTGCTGGCCGGCTGCGCTACCAG
CGCCTCGATAGACGCGTCCAGCCTGCCGAAGGCGCCGCAAGCCTTCAAGGAAAACGCCAGCCAGGTCGCTTCCGCAACGG
CCACCTCCGCCCAGGTGCAATCCCAGGGCACCTGGTGGAAGGTGTTTGCCGACCCCGTGCTGGACCAGCTGGTGGAGCAG
GCCGGCCGCAACAACAACAGCGTGCAAGTCGCGGCCGGGCGCCTGGCCCAGGCGAGGGCGATTGCCCGCAACACCGACGC
CGACCGCCTGCCTCAGGTCGGCCTGGGCGCCGGCGCCACCCGCCAGAGCAACGCCACCACCGGCAATGTGCCGCAAACCG
TAGTGAGCGCCAATGCCAACTTCTCTTACGAAGTGGACCTGTTCGGCAAGCTCTCGCTGAATTCCGACGCCGCCTCGCTG
GACGCCCAGTCCCGCGAAGCGCTGCTGCGCAGCACGAAATTGCTGGTGCAGGCCGATGTGGCCCAGACCTACCTGAACCT
GCGCGCACTTGACACCGAACGCTCGCTGGTGCGTGACACGCTGCAGGCCTACCGCAACACCCTGCGCCTGACCGAAGTGC
GCTTCAAGGAGGGCGACGTGGCCGAGCTCGACGTGGCCCGCGTGCGCGCCGAAGTCGCTTCCACGGAGTCGGAGGCGCTG
GCGCTGGACCGGCGCCGCGCCGAACTCGAGCATGCGCTGGCCGTGCTGGTGGGCGAGGTCGCCTCCAGCTTCCAGGTGGC
GCCCGCCGAATGGACCACGGCGCTGCCGGTCATCCCGGCCGGCGTGCCCAGCACCGTGCTGGTTCGCCGCCCCGATGTGT
CGGCGGCACAAAGCACCATGCAGGCGGCACAGGCCCGTGCCGGTGTGGCCAAGGCCGCCTGGTTCCCCACGTTCTCGCTG
ACAGCCACGGGCGGCTATGCGGCACCGGAGATCAGCGACCTGTTCAAGCTCTCCACCCGCGCCTGGGGCGTGGGCGCGTT
GTTGTCGCTGCCGATCTTTGACGGCGGCCGCCGCGAAGCCGGCGTGCAGAACGCCAATGCCGAACTCGACATCGCATTGG
CCAACTACCGCGAACAGATCCTGGTGGCCTTCAAGGATGTGGAAGACCAGCTGGCGGCGCTGCGCTTGCTGGCCGACCAG
TCCGAAGCGCAGTCGCGCTCGGTGGCCTCCGCTACCCGCGCCACGGTGCTGTCGGACTCGCGCTACCGCAACGGCTTCGT
CAGCCAGCTCGAGTTGCTGGACGCCCAGCGCAGCGAACTGCGCAACCGGCGGCAGGCGTTGCAGGTGAAGGCATCGCAGT
ACCTGGCCACGGTGGGGCTGATCCGCGCGTTGGGTGGTGGCTGGAGCACCTAA

Upstream 100 bases:

>100_bases
GCCTTCGCGGGCAGCGGCGGTGCCGGCGCGCATGCCCAGCCGGCGGCGCCCCTCAAGCATCACGAATAACAAGAAAACAA
GAAAGTCAAAGGAGTTCGTC

Downstream 100 bases:

>100_bases
ACACCCCTATCGGCGTTCACTGCGTGTAACGCCTTTTCCCCTTGCAGGGGACAGGCGCCTGTGGGCCGGCGAAGCCGGAC
CCCGGCCCCTGCTGGGGAAG

Product: RND efflux system outer membrane lipoprotein NodT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MNRIHSTLRMSLAPLVAALVLAGCATSASIDASSLPKAPQAFKENASQVASATATSAQVQSQGTWWKVFADPVLDQLVEQ
AGRNNNSVQVAAGRLAQARAIARNTDADRLPQVGLGAGATRQSNATTGNVPQTVVSANANFSYEVDLFGKLSLNSDAASL
DAQSREALLRSTKLLVQADVAQTYLNLRALDTERSLVRDTLQAYRNTLRLTEVRFKEGDVAELDVARVRAEVASTESEAL
ALDRRRAELEHALAVLVGEVASSFQVAPAEWTTALPVIPAGVPSTVLVRRPDVSAAQSTMQAAQARAGVAKAAWFPTFSL
TATGGYAAPEISDLFKLSTRAWGVGALLSLPIFDGGRREAGVQNANAELDIALANYREQILVAFKDVEDQLAALRLLADQ
SEAQSRSVASATRATVLSDSRYRNGFVSQLELLDAQRSELRNRRQALQVKASQYLATVGLIRALGGGWST

Sequences:

>Translated_470_residues
MNRIHSTLRMSLAPLVAALVLAGCATSASIDASSLPKAPQAFKENASQVASATATSAQVQSQGTWWKVFADPVLDQLVEQ
AGRNNNSVQVAAGRLAQARAIARNTDADRLPQVGLGAGATRQSNATTGNVPQTVVSANANFSYEVDLFGKLSLNSDAASL
DAQSREALLRSTKLLVQADVAQTYLNLRALDTERSLVRDTLQAYRNTLRLTEVRFKEGDVAELDVARVRAEVASTESEAL
ALDRRRAELEHALAVLVGEVASSFQVAPAEWTTALPVIPAGVPSTVLVRRPDVSAAQSTMQAAQARAGVAKAAWFPTFSL
TATGGYAAPEISDLFKLSTRAWGVGALLSLPIFDGGRREAGVQNANAELDIALANYREQILVAFKDVEDQLAALRLLADQ
SEAQSRSVASATRATVLSDSRYRNGFVSQLELLDAQRSELRNRRQALQVKASQYLATVGLIRALGGGWST
>Mature_470_residues
MNRIHSTLRMSLAPLVAALVLAGCATSASIDASSLPKAPQAFKENASQVASATATSAQVQSQGTWWKVFADPVLDQLVEQ
AGRNNNSVQVAAGRLAQARAIARNTDADRLPQVGLGAGATRQSNATTGNVPQTVVSANANFSYEVDLFGKLSLNSDAASL
DAQSREALLRSTKLLVQADVAQTYLNLRALDTERSLVRDTLQAYRNTLRLTEVRFKEGDVAELDVARVRAEVASTESEAL
ALDRRRAELEHALAVLVGEVASSFQVAPAEWTTALPVIPAGVPSTVLVRRPDVSAAQSTMQAAQARAGVAKAAWFPTFSL
TATGGYAAPEISDLFKLSTRAWGVGALLSLPIFDGGRREAGVQNANAELDIALANYREQILVAFKDVEDQLAALRLLADQ
SEAQSRSVASATRATVLSDSRYRNGFVSQLELLDAQRSELRNRRQALQVKASQYLATVGLIRALGGGWST

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=461, Percent_Identity=31.6702819956616, Blast_Score=201, Evalue=9e-53,
Organism=Escherichia coli, GI2367348, Length=469, Percent_Identity=25.5863539445629, Blast_Score=121, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 50083; Mature: 50083

Theoretical pI: Translated: 8.94; Mature: 8.94

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRIHSTLRMSLAPLVAALVLAGCATSASIDASSLPKAPQAFKENASQVASATATSAQVQ
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
SQGTWWKVFADPVLDQLVEQAGRNNNSVQVAAGRLAQARAIARNTDADRLPQVGLGAGAT
CCCCEEEEHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHCCCCHHHCCCCCCCCCCC
RQSNATTGNVPQTVVSANANFSYEVDLFGKLSLNSDAASLDAQSREALLRSTKLLVQADV
CCCCCCCCCCCHHHEECCCCCEEEEEEEEEEECCCCHHHCCHHHHHHHHHHHHHHHHHHH
AQTYLNLRALDTERSLVRDTLQAYRNTLRLTEVRFKEGDVAELDVARVRAEVASTESEAL
HHHHHHHHEECHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHCCCHHHH
ALDRRRAELEHALAVLVGEVASSFQVAPAEWTTALPVIPAGVPSTVLVRRPDVSAAQSTM
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHH
QAAQARAGVAKAAWFPTFSLTATGGYAAPEISDLFKLSTRAWGVGALLSLPIFDGGRREA
HHHHHHCCHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCC
GVQNANAELDIALANYREQILVAFKDVEDQLAALRLLADQSEAQSRSVASATRATVLSDS
CCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RYRNGFVSQLELLDAQRSELRNRRQALQVKASQYLATVGLIRALGGGWST
HHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNRIHSTLRMSLAPLVAALVLAGCATSASIDASSLPKAPQAFKENASQVASATATSAQVQ
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
SQGTWWKVFADPVLDQLVEQAGRNNNSVQVAAGRLAQARAIARNTDADRLPQVGLGAGAT
CCCCEEEEHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHCCCCHHHCCCCCCCCCCC
RQSNATTGNVPQTVVSANANFSYEVDLFGKLSLNSDAASLDAQSREALLRSTKLLVQADV
CCCCCCCCCCCHHHEECCCCCEEEEEEEEEEECCCCHHHCCHHHHHHHHHHHHHHHHHHH
AQTYLNLRALDTERSLVRDTLQAYRNTLRLTEVRFKEGDVAELDVARVRAEVASTESEAL
HHHHHHHHEECHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHCCCHHHH
ALDRRRAELEHALAVLVGEVASSFQVAPAEWTTALPVIPAGVPSTVLVRRPDVSAAQSTM
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHH
QAAQARAGVAKAAWFPTFSLTATGGYAAPEISDLFKLSTRAWGVGALLSLPIFDGGRREA
HHHHHHCCHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCC
GVQNANAELDIALANYREQILVAFKDVEDQLAALRLLADQSEAQSRSVASATRATVLSDS
CCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RYRNGFVSQLELLDAQRSELRNRRQALQVKASQYLATVGLIRALGGGWST
HHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]