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The map label for this gene is dusC [H]

Identifier: 91789280

GI number: 91789280

Start: 3622617

End: 3623651

Strand: Reverse

Name: dusC [H]

Synonym: Bpro_3425

Alternate gene names: 91789280

Gene position: 3623651-3622617 (Counterclockwise)

Preceding gene: 91789281

Following gene: 91789272

Centisome position: 69.68

GC content: 68.21

Gene sequence:

>1035_bases
ATGAAAGCTGAACCGCACGCCACAGTCTTGCTGGCACCGATGGAAGGCCTGCTGGACTTTGTCCTGCGCGACATCCTGAC
GCGGGTCGGCGGCATAGACCGCTGCGTCTCCGAATTCATCCGCATCACTGACCAGCTCTTGCCCGAGCGCGTGTTCACCC
GCATCGTGCCCGAGCTGCACAACGGTGGCCGCACGCTGGCCGGCGTGCCGGTGCGCGCGCAACTGCTCGGTTCTGACCCG
GCCTGCATGGCCGAGAACGCGGCGCGGCTGGCTACGCTCGGCCCGGCAGGCATTGACCTCAATTTCGGTTGCCCCGCCGC
TGTCGTCAACCGCCACGGCGGCGGTGCATCCCTGCTCAAGGAGCCCGAAACCCTGTTGGCCATCGTCAGCGCGGTGCGCC
GCGCCGTGCCGGCTGGCATGCCGGTTTCGGCCAAGATGCGGCTGGGCTACAACGACGACTCGCGTGCGGTCGAATGCGCA
CTGGCCATTGCCGAAGGCGGCGCCGGCGAGCTGGTGGTGCATGCCCGCACCAAGGCGCAGGCCTACAAGCCGCCCGCCTA
CTGGGAGCGCATTGCCGACATCCGCGCCGCGGTGCAGATACCCGTTGTCGCCAATGGCGAAATCTGGACGGTGGACGATG
CGCGCCGCTGCCGCGAAGCCTCGGGCTGCAGCATGCTGATGATTGGCCGCGGCGCCGTGGCAGACCCTGGCCTGGGGCTG
GCCATCAAGGCGGCTATGAGTCACCCGGCCGCTGGCGGGCAGGAGCCGGGCATCACGTGGGCAGAACTTGTGCCGCTGCT
GGCTGATTTCTGGCACGTGGTGTGCGCCCGCCTGGATGCGCGTGCCCGGCCCGGCCGGCTCAAGCAGTGGCTGAATTTTT
TGCGCCGCCGTTTTCCGCAAGCCGAAGCCGCCTACCAGGTGCTCAAGACCATCACCGACCCGGCCTTGATTGACGAGTGG
CTGGCAGCCTTGGTGGAGGCGGACGCCCGGCGCAGCGCTGACTTGCTGGAGCGCGCTGAACTCCTGCCTGCCTGA

Upstream 100 bases:

>100_bases
AGCCTCGGACGCGACCGACCCGCTGCATGCGGCGCGCGCGGCAGAAGTCACGTCGCTGTGGCGGCTGGCGGCCAAATAGA
AGCTTGAGAAGTTTGACGAG

Downstream 100 bases:

>100_bases
GCGTTGCGCATCGTGCTGCCGGTCTCGGCTGCCGGTGGCCCGGCCGCCTTGTCATCGGGGTTGTGAACCTTGTTTTCGGG
CGATTTTCGCCACCTCCGAA

Product: dihydrouridine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MKAEPHATVLLAPMEGLLDFVLRDILTRVGGIDRCVSEFIRITDQLLPERVFTRIVPELHNGGRTLAGVPVRAQLLGSDP
ACMAENAARLATLGPAGIDLNFGCPAAVVNRHGGGASLLKEPETLLAIVSAVRRAVPAGMPVSAKMRLGYNDDSRAVECA
LAIAEGGAGELVVHARTKAQAYKPPAYWERIADIRAAVQIPVVANGEIWTVDDARRCREASGCSMLMIGRGAVADPGLGL
AIKAAMSHPAAGGQEPGITWAELVPLLADFWHVVCARLDARARPGRLKQWLNFLRRRFPQAEAAYQVLKTITDPALIDEW
LAALVEADARRSADLLERAELLPA

Sequences:

>Translated_344_residues
MKAEPHATVLLAPMEGLLDFVLRDILTRVGGIDRCVSEFIRITDQLLPERVFTRIVPELHNGGRTLAGVPVRAQLLGSDP
ACMAENAARLATLGPAGIDLNFGCPAAVVNRHGGGASLLKEPETLLAIVSAVRRAVPAGMPVSAKMRLGYNDDSRAVECA
LAIAEGGAGELVVHARTKAQAYKPPAYWERIADIRAAVQIPVVANGEIWTVDDARRCREASGCSMLMIGRGAVADPGLGL
AIKAAMSHPAAGGQEPGITWAELVPLLADFWHVVCARLDARARPGRLKQWLNFLRRRFPQAEAAYQVLKTITDPALIDEW
LAALVEADARRSADLLERAELLPA
>Mature_344_residues
MKAEPHATVLLAPMEGLLDFVLRDILTRVGGIDRCVSEFIRITDQLLPERVFTRIVPELHNGGRTLAGVPVRAQLLGSDP
ACMAENAARLATLGPAGIDLNFGCPAAVVNRHGGGASLLKEPETLLAIVSAVRRAVPAGMPVSAKMRLGYNDDSRAVECA
LAIAEGGAGELVVHARTKAQAYKPPAYWERIADIRAAVQIPVVANGEIWTVDDARRCREASGCSMLMIGRGAVADPGLGL
AIKAAMSHPAAGGQEPGITWAELVPLLADFWHVVCARLDARARPGRLKQWLNFLRRRFPQAEAAYQVLKTITDPALIDEW
LAALVEADARRSADLLERAELLPA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusC subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=231, Percent_Identity=29.004329004329, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI40807366, Length=242, Percent_Identity=30.1652892561983, Blast_Score=85, Evalue=9e-17,
Organism=Homo sapiens, GI239788483, Length=236, Percent_Identity=27.9661016949153, Blast_Score=82, Evalue=8e-16,
Organism=Homo sapiens, GI239788462, Length=236, Percent_Identity=27.9661016949153, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI8923374, Length=250, Percent_Identity=28, Blast_Score=72, Evalue=7e-13,
Organism=Escherichia coli, GI1788462, Length=311, Percent_Identity=45.9807073954984, Blast_Score=275, Evalue=4e-75,
Organism=Escherichia coli, GI1789660, Length=237, Percent_Identity=33.3333333333333, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI145693211, Length=231, Percent_Identity=29.8701298701299, Blast_Score=71, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17507177, Length=154, Percent_Identity=37.012987012987, Blast_Score=81, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI25144369, Length=214, Percent_Identity=28.0373831775701, Blast_Score=81, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17543114, Length=150, Percent_Identity=28.6666666666667, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6323560, Length=277, Percent_Identity=22.0216606498195, Blast_Score=80, Evalue=5e-16,
Organism=Drosophila melanogaster, GI19921524, Length=172, Percent_Identity=33.7209302325581, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24585320, Length=237, Percent_Identity=29.957805907173, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24580595, Length=173, Percent_Identity=32.9479768786127, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI19920448, Length=173, Percent_Identity=32.9479768786127, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI45549423, Length=162, Percent_Identity=29.0123456790123, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 36883; Mature: 36883

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAEPHATVLLAPMEGLLDFVLRDILTRVGGIDRCVSEFIRITDQLLPERVFTRIVPELH
CCCCCCCEEEECCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NGGRTLAGVPVRAQLLGSDPACMAENAARLATLGPAGIDLNFGCPAAVVNRHGGGASLLK
CCCCEEECCCCEEHCCCCCCHHHHHCCHHHHCCCCCCEECCCCCCHHHHHCCCCCHHHHH
EPETLLAIVSAVRRAVPAGMPVSAKMRLGYNDDSRAVECALAIAEGGAGELVVHARTKAQ
CHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCEEEEECHHHH
AYKPPAYWERIADIRAAVQIPVVANGEIWTVDDARRCREASGCSMLMIGRGAVADPGLGL
CCCCHHHHHHHHHHHHHEEEEEEECCCEEECCHHHHHHHHCCCCEEEECCCCCCCCCCCH
AIKAAMSHPAAGGQEPGITWAELVPLLADFWHVVCARLDARARPGRLKQWLNFLRRRFPQ
HHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCC
AEAAYQVLKTITDPALIDEWLAALVEADARRSADLLERAELLPA
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKAEPHATVLLAPMEGLLDFVLRDILTRVGGIDRCVSEFIRITDQLLPERVFTRIVPELH
CCCCCCCEEEECCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NGGRTLAGVPVRAQLLGSDPACMAENAARLATLGPAGIDLNFGCPAAVVNRHGGGASLLK
CCCCEEECCCCEEHCCCCCCHHHHHCCHHHHCCCCCCEECCCCCCHHHHHCCCCCHHHHH
EPETLLAIVSAVRRAVPAGMPVSAKMRLGYNDDSRAVECALAIAEGGAGELVVHARTKAQ
CHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCEEEEECHHHH
AYKPPAYWERIADIRAAVQIPVVANGEIWTVDDARRCREASGCSMLMIGRGAVADPGLGL
CCCCHHHHHHHHHHHHHEEEEEEECCCEEECCHHHHHHHHCCCCEEEECCCCCCCCCCCH
AIKAAMSHPAAGGQEPGITWAELVPLLADFWHVVCARLDARARPGRLKQWLNFLRRRFPQ
HHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCC
AEAAYQVLKTITDPALIDEWLAALVEADARRSADLLERAELLPA
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]