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The map label for this gene is estA [H]

Identifier: 91789257

GI number: 91789257

Start: 3599035

End: 3599697

Strand: Reverse

Name: estA [H]

Synonym: Bpro_3402

Alternate gene names: 91789257

Gene position: 3599697-3599035 (Counterclockwise)

Preceding gene: 91789259

Following gene: 91789254

Centisome position: 69.22

GC content: 65.61

Gene sequence:

>663_bases
ATGTCCCTTGAAGTTCTTGAAGCCCAGACCGGCAACAACCCGGTCGCCACCATTGTCATCATGCACGGCCTGGGTGCCGA
CGGTCGCGATTTTGTGCCGATTGCCGAGCAACTGGATTTGTCCAGTGTGGGGCCGGTGCGCTTTCTCTTTCCCAGTGCGC
CCGTGATGCCCGTCACCATCAATGGGGGCTATGTCATGCCCGCCTGGTACGACATTCTGGGCGCCGACCTGGCCAAACGT
GAGGACGAGGCCGGGCTGCGCCAGTCGCAGGCTTCGATTGAAGCCTTGCTCGCACATGAAAAGTCGCGGGGTATACCGGC
CAATCGCATCGTGGTGGCCGGCTTCTCGCAGGGCTGCGCCCTGGCCCTCATGACGGGCTTGCGGCACGGTGAACGGCTGG
CCGGTATTGCGGGCCTGTCGGGCTACCTGCCGCTGGCGGACAAGACCGCTGCCGAGCGCAGCGCTGCCAGCCAGGGCCTG
CCCATCTTCCTGGCTCATGGCTCCCACGACGGCGTGGTGCCGCTGCCGCGCGCCACGGCCTCGCGCGATGCACTCACGGC
CCTGGGCTACCCGGTTGAATGGCACGAATACCGCATGGAGCATTCCGTCTGCCCCGAAGAGGTTGTGGATCTGGAGCGCT
GGCTGCGGCGGGTGCTTGCCTGA

Upstream 100 bases:

>100_bases
TTGCCAGGCAGTTCCTTCGCTGTTCAGCCGCTGACCTGCAGCGAGCCCGCAACGCAGCAGCATCCCTGCGCCAACGTCAA
CCCTACAACCGGCAAGTCCC

Downstream 100 bases:

>100_bases
TCCAGGAGCAGGCAACGACTGCAACTCCGGTTCATTGGGTGCGAAATGCTATTGAAAATATAGCAATCCGATGACGGCCC
GGTGGAGCGTTCAGGCCGGC

Product: carboxylesterase

Products: NA

Alternate protein names: Esterase I [H]

Number of amino acids: Translated: 220; Mature: 219

Protein sequence:

>220_residues
MSLEVLEAQTGNNPVATIVIMHGLGADGRDFVPIAEQLDLSSVGPVRFLFPSAPVMPVTINGGYVMPAWYDILGADLAKR
EDEAGLRQSQASIEALLAHEKSRGIPANRIVVAGFSQGCALALMTGLRHGERLAGIAGLSGYLPLADKTAAERSAASQGL
PIFLAHGSHDGVVPLPRATASRDALTALGYPVEWHEYRMEHSVCPEEVVDLERWLRRVLA

Sequences:

>Translated_220_residues
MSLEVLEAQTGNNPVATIVIMHGLGADGRDFVPIAEQLDLSSVGPVRFLFPSAPVMPVTINGGYVMPAWYDILGADLAKR
EDEAGLRQSQASIEALLAHEKSRGIPANRIVVAGFSQGCALALMTGLRHGERLAGIAGLSGYLPLADKTAAERSAASQGL
PIFLAHGSHDGVVPLPRATASRDALTALGYPVEWHEYRMEHSVCPEEVVDLERWLRRVLA
>Mature_219_residues
SLEVLEAQTGNNPVATIVIMHGLGADGRDFVPIAEQLDLSSVGPVRFLFPSAPVMPVTINGGYVMPAWYDILGADLAKRE
DEAGLRQSQASIEALLAHEKSRGIPANRIVVAGFSQGCALALMTGLRHGERLAGIAGLSGYLPLADKTAAERSAASQGLP
IFLAHGSHDGVVPLPRATASRDALTALGYPVEWHEYRMEHSVCPEEVVDLERWLRRVLA

Specific function: Hydrolyzes carboxylic ester bonds with relatively broad substrate specificity [H]

COG id: COG0400

COG function: function code R; Predicted esterase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase 2 family [H]

Homologues:

Organism=Homo sapiens, GI5453722, Length=209, Percent_Identity=36.3636363636364, Blast_Score=137, Evalue=6e-33,
Organism=Homo sapiens, GI9966764, Length=208, Percent_Identity=37.0192307692308, Blast_Score=137, Evalue=7e-33,
Organism=Homo sapiens, GI20270341, Length=205, Percent_Identity=24.8780487804878, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17508057, Length=209, Percent_Identity=36.8421052631579, Blast_Score=134, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6323147, Length=213, Percent_Identity=34.7417840375587, Blast_Score=122, Evalue=5e-29,
Organism=Drosophila melanogaster, GI45553055, Length=206, Percent_Identity=35.9223300970874, Blast_Score=132, Evalue=2e-31,
Organism=Drosophila melanogaster, GI45553057, Length=206, Percent_Identity=35.9223300970874, Blast_Score=132, Evalue=2e-31,
Organism=Drosophila melanogaster, GI21357257, Length=206, Percent_Identity=35.9223300970874, Blast_Score=132, Evalue=2e-31,
Organism=Drosophila melanogaster, GI161078179, Length=215, Percent_Identity=28.3720930232558, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003140 [H]

Pfam domain/function: PF02230 Abhydrolase_2 [H]

EC number: =3.1.1.1 [H]

Molecular weight: Translated: 23526; Mature: 23395

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLEVLEAQTGNNPVATIVIMHGLGADGRDFVPIAEQLDLSSVGPVRFLFPSAPVMPVTI
CCCEEEECCCCCCCEEEEEEEECCCCCCCCCCCHHHHCCCCCCCCEEEECCCCCEEEEEE
NGGYVMPAWYDILGADLAKREDEAGLRQSQASIEALLAHEKSRGIPANRIVVAGFSQGCA
CCCEECHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHH
LALMTGLRHGERLAGIAGLSGYLPLADKTAAERSAASQGLPIFLAHGSHDGVVPLPRATA
HHHHHHHCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCEECCCCCC
SRDALTALGYPVEWHEYRMEHSVCPEEVVDLERWLRRVLA
CCHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SLEVLEAQTGNNPVATIVIMHGLGADGRDFVPIAEQLDLSSVGPVRFLFPSAPVMPVTI
CCEEEECCCCCCCEEEEEEEECCCCCCCCCCCHHHHCCCCCCCCEEEECCCCCEEEEEE
NGGYVMPAWYDILGADLAKREDEAGLRQSQASIEALLAHEKSRGIPANRIVVAGFSQGCA
CCCEECHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHH
LALMTGLRHGERLAGIAGLSGYLPLADKTAAERSAASQGLPIFLAHGSHDGVVPLPRATA
HHHHHHHCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCEECCCCCC
SRDALTALGYPVEWHEYRMEHSVCPEEVVDLERWLRRVLA
CCHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA