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The map label for this gene is gshA [H]

Identifier: 91789254

GI number: 91789254

Start: 3594898

End: 3596511

Strand: Reverse

Name: gshA [H]

Synonym: Bpro_3399

Alternate gene names: 91789254

Gene position: 3596511-3594898 (Counterclockwise)

Preceding gene: 91789257

Following gene: 91789251

Centisome position: 69.16

GC content: 65.61

Gene sequence:

>1614_bases
ATGAACAAGCTGCAGGCGCCCGAGGGCACGCTTTTGCAGGAGCGCCTGAAAGCGCTCACCCCTGCCCGGCTGAAGGGCAT
GCGCCGCGGCATCGAGAAGGAAAGCCTGCGCGCCCAGCCCGGGGGCGGCCTGGCGCTCACGCCACACCCGGCCGCGCTCG
GCTCGGCGCTGACGCATCCCAGCATCACCACCGACTTCAGCGAGTCGCAGATCGAACTGGTGACGGGGGCACATCAAGAC
GCGGAAGCCACACTGAAAGAACTCACGCAGATCCACCAGTTCACCTACCGTGTGATGCAGGACCTCGGCGATGAAATGCT
CTGGGTTTCCAGCATGCCCTGCGGTTTGCCGACCGACGAGACCATTCCGATTGCCCGCTTCGGCTCGTCCAATGTCGGGC
GCGCCAAGAGCGTCTACCGCATGGGCCTGTCCCACCGCTATGGCCGGCGCATGCAAACCATCTCCGGCATTCACTACAAC
TGGTCGCTGCCCGAGGTCACCAGCGAGCAGTATTTTGCGCTGATCCGCAACTTCCGGCGCCACGCCTTTTTGCTGCTTTA
CCTGTTTGGTGCATCGCCCGCCGTGTGTTCGAGTTTTGTGGCCGGACGCCAGCACGAGCTGCAACAGCTGGCCGATCACA
CCATGTACATGCCGCACGGCACCTCGCTGCGCATGGGGCGCCTGGGCTACCAGAGCGACGCCCAGGCCTCGCTGGCCGTG
AGCTACAACAGCCTCGAAGGCTACGGCGCGTCCTTGCAGGATGCGCTCACCCGGCCCTATCCGGCCTACGAGGCGGTCGG
CATCCGCAACCCCGGCGGCGACTACAACCAGCTGGCCACCACGCTGCTGCAGATTGAAAACGAGTTCTACGGCACCATCC
GCCCCAAGCGCGTGATCTTCCCCGGCGAGCGCCCGCTGCATGCGCTGCGCGAGCGCGGCGTTGAATACATTGAGGTGCGC
TTGATGGACCTCGATCCGTTTGTTCCCGTGGGCATCACGGCGCAAACCATGCGGTTTCTGGATGTGTTTTTGCTGCACTG
CCTGCTCACTGACAGCCCGCCCGACACCCCTGCGGAAATTGCCGCGCTGGCGCGCAACCAGCACCGCGCCGCTGCCCGCG
GCCGCGAGCCCGGCCTGCTGCTGGAGCGGGACGGCCGCGAGGTGACGCTGGCGGAGTGGGGCACCGCGGTGGTCGCCGAA
TGCGCACCGATTGCCGCGGCGCTGGATGCCCTCCATGCGGGCGCACCGGGCGGTGCGCAATACAGCGAGGCGCTGGCCGC
AGCAAGCGCCGCCTTGCGCGAGCCCGACACCCTGCCATCAGCCCGTGTACTGGCTGCCATGGCCAAAGACTATGACAACT
CCTTTGTCCGATTTGCCCGGGCCCAATCCGAAAAAGCCCAGACCATCCTGCGAAGCCTGCCCCTGGCCGAAGCGCAGCAT
GCCCGTTTCACCGAACTGACCCGGCAATCCATCGCCGAGCAAAAGCAGATCGAAGCCTCGGACAGCATGCCCTTTGAGGT
GTATCGGCAGCAGTATGTGTCGGCGGAGCGCTTGGGCATACCGGGTATGCCTGATGCGCCGGATATGGCGGGCCGCGCAG
CGCTCGCAATCTGA

Upstream 100 bases:

>100_bases
AGAGTCCGGTAAACCGTGCTGGCAAGCGGCTTTTCGCCAGCGCGAAGCGCGGGCCTGTGATAACTTCCACGCCACGACAC
GGACCTGAGGGAATGACATC

Downstream 100 bases:

>100_bases
TCAGGCCGGTGGACTCGCGTCCCCCAGCTTGCGCAAGGCCTCTTCGGCCTGTTCGCACCAGTAGTCGACCGCGTTGTCAT
AGCTTTCCCACACGCAGCCA

Product: glutamate-cysteine ligase

Products: NA

Alternate protein names: Gamma-ECS; GCS; Gamma-glutamylcysteine synthetase [H]

Number of amino acids: Translated: 537; Mature: 537

Protein sequence:

>537_residues
MNKLQAPEGTLLQERLKALTPARLKGMRRGIEKESLRAQPGGGLALTPHPAALGSALTHPSITTDFSESQIELVTGAHQD
AEATLKELTQIHQFTYRVMQDLGDEMLWVSSMPCGLPTDETIPIARFGSSNVGRAKSVYRMGLSHRYGRRMQTISGIHYN
WSLPEVTSEQYFALIRNFRRHAFLLLYLFGASPAVCSSFVAGRQHELQQLADHTMYMPHGTSLRMGRLGYQSDAQASLAV
SYNSLEGYGASLQDALTRPYPAYEAVGIRNPGGDYNQLATTLLQIENEFYGTIRPKRVIFPGERPLHALRERGVEYIEVR
LMDLDPFVPVGITAQTMRFLDVFLLHCLLTDSPPDTPAEIAALARNQHRAAARGREPGLLLERDGREVTLAEWGTAVVAE
CAPIAAALDALHAGAPGGAQYSEALAAASAALREPDTLPSARVLAAMAKDYDNSFVRFARAQSEKAQTILRSLPLAEAQH
ARFTELTRQSIAEQKQIEASDSMPFEVYRQQYVSAERLGIPGMPDAPDMAGRAALAI

Sequences:

>Translated_537_residues
MNKLQAPEGTLLQERLKALTPARLKGMRRGIEKESLRAQPGGGLALTPHPAALGSALTHPSITTDFSESQIELVTGAHQD
AEATLKELTQIHQFTYRVMQDLGDEMLWVSSMPCGLPTDETIPIARFGSSNVGRAKSVYRMGLSHRYGRRMQTISGIHYN
WSLPEVTSEQYFALIRNFRRHAFLLLYLFGASPAVCSSFVAGRQHELQQLADHTMYMPHGTSLRMGRLGYQSDAQASLAV
SYNSLEGYGASLQDALTRPYPAYEAVGIRNPGGDYNQLATTLLQIENEFYGTIRPKRVIFPGERPLHALRERGVEYIEVR
LMDLDPFVPVGITAQTMRFLDVFLLHCLLTDSPPDTPAEIAALARNQHRAAARGREPGLLLERDGREVTLAEWGTAVVAE
CAPIAAALDALHAGAPGGAQYSEALAAASAALREPDTLPSARVLAAMAKDYDNSFVRFARAQSEKAQTILRSLPLAEAQH
ARFTELTRQSIAEQKQIEASDSMPFEVYRQQYVSAERLGIPGMPDAPDMAGRAALAI
>Mature_537_residues
MNKLQAPEGTLLQERLKALTPARLKGMRRGIEKESLRAQPGGGLALTPHPAALGSALTHPSITTDFSESQIELVTGAHQD
AEATLKELTQIHQFTYRVMQDLGDEMLWVSSMPCGLPTDETIPIARFGSSNVGRAKSVYRMGLSHRYGRRMQTISGIHYN
WSLPEVTSEQYFALIRNFRRHAFLLLYLFGASPAVCSSFVAGRQHELQQLADHTMYMPHGTSLRMGRLGYQSDAQASLAV
SYNSLEGYGASLQDALTRPYPAYEAVGIRNPGGDYNQLATTLLQIENEFYGTIRPKRVIFPGERPLHALRERGVEYIEVR
LMDLDPFVPVGITAQTMRFLDVFLLHCLLTDSPPDTPAEIAALARNQHRAAARGREPGLLLERDGREVTLAEWGTAVVAE
CAPIAAALDALHAGAPGGAQYSEALAAASAALREPDTLPSARVLAAMAKDYDNSFVRFARAQSEKAQTILRSLPLAEAQH
ARFTELTRQSIAEQKQIEASDSMPFEVYRQQYVSAERLGIPGMPDAPDMAGRAALAI

Specific function: Glutathione biosynthesis; first step. [C]

COG id: COG2918

COG function: function code H; Gamma-glutamylcysteine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate--cysteine ligase type 1 family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789044, Length=513, Percent_Identity=38.9863547758285, Blast_Score=359, Evalue=1e-100,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007370
- InterPro:   IPR006334 [H]

Pfam domain/function: PF04262 Glu_cys_ligase [H]

EC number: =6.3.2.2 [H]

Molecular weight: Translated: 58926; Mature: 58926

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKLQAPEGTLLQERLKALTPARLKGMRRGIEKESLRAQPGGGLALTPHPAALGSALTHP
CCCCCCCCCHHHHHHHHHHCHHHHHHHHHCCCHHHHCCCCCCCEEECCCHHHHHHHHCCC
SITTDFSESQIELVTGAHQDAEATLKELTQIHQFTYRVMQDLGDEMLWVSSMPCGLPTDE
CCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCC
TIPIARFGSSNVGRAKSVYRMGLSHRYGRRMQTISGIHYNWSLPEVTSEQYFALIRNFRR
CCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHH
HAFLLLYLFGASPAVCSSFVAGRQHELQQLADHTMYMPHGTSLRMGRLGYQSDAQASLAV
HHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHCEECCCCCCEEECCCCCCCCCCEEEEE
SYNSLEGYGASLQDALTRPYPAYEAVGIRNPGGDYNQLATTLLQIENEFYGTIRPKRVIF
EECCCCCCCCCHHHHHCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEC
PGERPLHALRERGVEYIEVRLMDLDPFVPVGITAQTMRFLDVFLLHCLLTDSPPDTPAEI
CCCCHHHHHHHCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHH
AALARNQHRAAARGREPGLLLERDGREVTLAEWGTAVVAECAPIAAALDALHAGAPGGAQ
HHHHHHHHHHHHCCCCCCEEEECCCCEEEHHHHCHHHHHHHHHHHHHHHHHHCCCCCCHH
YSEALAAASAALREPDTLPSARVLAAMAKDYDNSFVRFARAQSEKAQTILRSLPLAEAQH
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
ARFTELTRQSIAEQKQIEASDSMPFEVYRQQYVSAERLGIPGMPDAPDMAGRAALAI
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCC
>Mature Secondary Structure
MNKLQAPEGTLLQERLKALTPARLKGMRRGIEKESLRAQPGGGLALTPHPAALGSALTHP
CCCCCCCCCHHHHHHHHHHCHHHHHHHHHCCCHHHHCCCCCCCEEECCCHHHHHHHHCCC
SITTDFSESQIELVTGAHQDAEATLKELTQIHQFTYRVMQDLGDEMLWVSSMPCGLPTDE
CCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCC
TIPIARFGSSNVGRAKSVYRMGLSHRYGRRMQTISGIHYNWSLPEVTSEQYFALIRNFRR
CCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHH
HAFLLLYLFGASPAVCSSFVAGRQHELQQLADHTMYMPHGTSLRMGRLGYQSDAQASLAV
HHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHCEECCCCCCEEECCCCCCCCCCEEEEE
SYNSLEGYGASLQDALTRPYPAYEAVGIRNPGGDYNQLATTLLQIENEFYGTIRPKRVIF
EECCCCCCCCCHHHHHCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEC
PGERPLHALRERGVEYIEVRLMDLDPFVPVGITAQTMRFLDVFLLHCLLTDSPPDTPAEI
CCCCHHHHHHHCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHH
AALARNQHRAAARGREPGLLLERDGREVTLAEWGTAVVAECAPIAAALDALHAGAPGGAQ
HHHHHHHHHHHHCCCCCCEEEECCCCEEEHHHHCHHHHHHHHHHHHHHHHHHCCCCCCHH
YSEALAAASAALREPDTLPSARVLAAMAKDYDNSFVRFARAQSEKAQTILRSLPLAEAQH
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
ARFTELTRQSIAEQKQIEASDSMPFEVYRQQYVSAERLGIPGMPDAPDMAGRAALAI
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA