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The map label for this gene is trmJ [H]

Identifier: 91789152

GI number: 91789152

Start: 3487861

End: 3488667

Strand: Reverse

Name: trmJ [H]

Synonym: Bpro_3292

Alternate gene names: 91789152

Gene position: 3488667-3487861 (Counterclockwise)

Preceding gene: 91789166

Following gene: 91789151

Centisome position: 67.09

GC content: 63.44

Gene sequence:

>807_bases
ATGAAAACCCGTTTTGTCCTGATCAACACCAGCCACGCCGGCAATGTGGGCGCTGCCGCGCGCGCCATGAAGGTCATGGG
CTTTGACGACCTGGTGCTGGTGGCGCCGCGCTGGGCGAACGTGCTGAACCGTGAAGAAACCATCCAGCGGGCCAGCGGCG
CGCTCGACGTCCTGAAAAACGCCCGTATCGTGGCCACGCTGGATGAGGCGCTCGATGGCATGTCGCACCTGTGCGCCACC
GCCATGACGCCGCGCGACTTTGGCCCGCCAACCCGCGCGCCGCGCGAGCACTTCAGCAAATTGCTATCAAACGATGAGCC
CTCGTCGCCCGAAAATAAAGGGCTGCAGCCCGATTCGGTGGCCTTCCTGTTTGGTTCCGAACGGTTTGGCATGCAAAACG
ACGATGTGTACCGCTGCCATGTGGCCTTGAGCATTCCAACCGACCCGAAGTTTGGCTCGCTCAACCTCGGGGCCGCGGTG
CAGCTGATTGCCTATGACTGGCGCCAGGCGCTGGGCGGTTTTGCCGTGCAGCCGGCTACACCCGAGCCCAAACTGGCGGA
CGCCGCCGAGGTGGCCGGCATGCTCGGCCACTGGGAAGAAGCGCTCACCGACATTGGTTTTCTGGACCCGGCCTCACCGA
AAAAACTGATGCCGCGACTGAACCAGCTTTTCAACCGGGCGCAGCTCAGCCCTGAAGAGATCCACATCCTGCGCGGTGTG
GCCAAGGCCATGTCGAGGACGGCGAATAAAGCCGTGCCGTCCGCGGTCAAAACGCCTGCGCCAGCAGACACGCTGGACCC
CCAGTAG

Upstream 100 bases:

>100_bases
CCGGTAACCGGGGCAGGCGACAATATCCTCATTTTACCGGCATCGCCCGAATAGCCCCCCGAAACACGACGGCGACAGCC
TTCCACTGCAACATTTACCG

Downstream 100 bases:

>100_bases
ACTCGGAGCCATGTTTTCCAGACTTCGCTCCGACATCCAGTGCATTCTTTTGCGGGACCCCGCCGCCCGTACGGCCTGGG
AAGTGCTGACCTGCTACCCG

Product: RNA methyltransferase TrmH

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MKTRFVLINTSHAGNVGAAARAMKVMGFDDLVLVAPRWANVLNREETIQRASGALDVLKNARIVATLDEALDGMSHLCAT
AMTPRDFGPPTRAPREHFSKLLSNDEPSSPENKGLQPDSVAFLFGSERFGMQNDDVYRCHVALSIPTDPKFGSLNLGAAV
QLIAYDWRQALGGFAVQPATPEPKLADAAEVAGMLGHWEEALTDIGFLDPASPKKLMPRLNQLFNRAQLSPEEIHILRGV
AKAMSRTANKAVPSAVKTPAPADTLDPQ

Sequences:

>Translated_268_residues
MKTRFVLINTSHAGNVGAAARAMKVMGFDDLVLVAPRWANVLNREETIQRASGALDVLKNARIVATLDEALDGMSHLCAT
AMTPRDFGPPTRAPREHFSKLLSNDEPSSPENKGLQPDSVAFLFGSERFGMQNDDVYRCHVALSIPTDPKFGSLNLGAAV
QLIAYDWRQALGGFAVQPATPEPKLADAAEVAGMLGHWEEALTDIGFLDPASPKKLMPRLNQLFNRAQLSPEEIHILRGV
AKAMSRTANKAVPSAVKTPAPADTLDPQ
>Mature_268_residues
MKTRFVLINTSHAGNVGAAARAMKVMGFDDLVLVAPRWANVLNREETIQRASGALDVLKNARIVATLDEALDGMSHLCAT
AMTPRDFGPPTRAPREHFSKLLSNDEPSSPENKGLQPDSVAFLFGSERFGMQNDDVYRCHVALSIPTDPKFGSLNLGAAV
QLIAYDWRQALGGFAVQPATPEPKLADAAEVAGMLGHWEEALTDIGFLDPASPKKLMPRLNQLFNRAQLSPEEIHILRGV
AKAMSRTANKAVPSAVKTPAPADTLDPQ

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=256, Percent_Identity=37.890625, Blast_Score=150, Evalue=9e-38,
Organism=Escherichia coli, GI1790865, Length=230, Percent_Identity=29.5652173913043, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 28945; Mature: 28945

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTRFVLINTSHAGNVGAAARAMKVMGFDDLVLVAPRWANVLNREETIQRASGALDVLKN
CCCEEEEEECCCCCCHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHC
ARIVATLDEALDGMSHLCATAMTPRDFGPPTRAPREHFSKLLSNDEPSSPENKGLQPDSV
CEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCE
AFLFGSERFGMQNDDVYRCHVALSIPTDPKFGSLNLGAAVQLIAYDWRQALGGFAVQPAT
EEEECCCCCCCCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEECCCC
PEPKLADAAEVAGMLGHWEEALTDIGFLDPASPKKLMPRLNQLFNRAQLSPEEIHILRGV
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHH
AKAMSRTANKAVPSAVKTPAPADTLDPQ
HHHHHHHHHHHCCHHHCCCCCCCCCCCC
>Mature Secondary Structure
MKTRFVLINTSHAGNVGAAARAMKVMGFDDLVLVAPRWANVLNREETIQRASGALDVLKN
CCCEEEEEECCCCCCHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHC
ARIVATLDEALDGMSHLCATAMTPRDFGPPTRAPREHFSKLLSNDEPSSPENKGLQPDSV
CEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCE
AFLFGSERFGMQNDDVYRCHVALSIPTDPKFGSLNLGAAVQLIAYDWRQALGGFAVQPAT
EEEECCCCCCCCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEECCCC
PEPKLADAAEVAGMLGHWEEALTDIGFLDPASPKKLMPRLNQLFNRAQLSPEEIHILRGV
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHH
AKAMSRTANKAVPSAVKTPAPADTLDPQ
HHHHHHHHHHHCCHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]