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The map label for this gene is pmbA [H]

Identifier: 91789147

GI number: 91789147

Start: 3483014

End: 3484444

Strand: Reverse

Name: pmbA [H]

Synonym: Bpro_3287

Alternate gene names: 91789147

Gene position: 3484444-3483014 (Counterclockwise)

Preceding gene: 91789151

Following gene: 91789146

Centisome position: 67.01

GC content: 63.52

Gene sequence:

>1431_bases
ATGACCCAAAAGCCTTCCACCCGCCATTCCACCCCTTCAGCCGGCAGCGTTTCCGGCCTGAAGAGCCGCACCACCCGCCA
GCCTGAATCCGGTTTCAGCTACCACCGCGACTTTTTCGAGGAGCTGGTGGACACTGCGCTGGACCATGCGAAGAAGCTGG
GCGCCAGCGATGCGGCGGCCGAAGCGTCGGAGGGCTGCGGCCTGTCCGTCAGCGTGCGCAAGGGTGAACTCGAAAACGTG
GAACGTAACCGCGACAAGTCACTGGGTATCACGGTGTACATCGGCAAAAAGCGCGGCAACGCGGCCACCAGCGACTTTTC
CAAAGCTGCGATCCAGCAGACCGTGAAGGCGGCCTACGACATTGCGCGCTTTACCGCGGAAGATGAGACCTCGGGCCTGC
CCGACGACCGCGACATTGCCCGCGAGTACCCGGCGCTGGACCTGTTCCACCCCTGGGCCATCACGTCGGAGGAGGCGGCT
CACCTAGCCCTGCAATGCGAAGCCGCGGCCATGGGCACCAGCCGGCTGATCACCAACAGCGAGGGGGCTGGCGTGTCGGC
CCAGCAAAGCCATTTCTTCAGTGCCCATACCAAGGGATTTCGCGGCGGCTATGCCAGTTCGCGCCACAGCATTTCGGTGG
CGCCCATCGCGGGCAAGGGCAACCAGATGCAGCGCGACGCCTGGTACAGCTCCATGCGCGACGCCAACGAATTGGCCCGC
CCCGAGGCGGTGGGCCGTTATGCCGCCGAGCGCGCGCTGGCGCGCCTGAGAAGCCGGAAAATTCCCACCACGCAGTGCCC
GGTGCTGTTTGAATCACCTCTGGCGGCCGGCCTGCTGGGCGGTTTTGTACAGGCTGTCAGCGGCGGTGCGCTTTACCGCA
AAAGCACGTTTTTGGCGAACAGCCTGGGCAAGGCCGCTTTCCCCAGGCACATCGACATTTACGAAGACCCGCACCTGATG
AAGGGCAAGGGCAGCGCGCCGTTTGACGAAGAGGGTGTGAAAACCAGCGCCCGCAGGGTGGTGGACGCCGGGCGGGTGGA
GGGGTACTTCCTGAGCACCTACTCAGCGCGCAAGCTGGGCATGCGCACCACCGGCAATGCCGGCGGCTCGCACAACCTCA
CGCTGACCAGCCGCCACACGCGGACAGGCGATGACCTGGACGCCATGCTGCGCAAGCTGGGCCGGGGGCTGTTTGTGACC
GAACTGATGGGGCAAGGCGTGAACTACGTGACCGGCGACTACTCGCGCGGTGCGTCCGGCTTCTGGGTCGAAAACGGCCG
GATTGCCTTCCCCGTTGAAGAAATCACCATTGCCGGTAACCTCAAGGCCATGCTGATGGGGATTGAGGCCGTGGGGGCCG
ATATGTATACTTATGGGTCAAAATCAGTCGGCTCAATGTTAGTAAATCGTATGAAAGTGGCGGGTACTTGA

Upstream 100 bases:

>100_bases
AGCGATTTCTTCGGGTTTGACGAAGCGGCCGTTGGACCAGTATCCCTTGATGGCCTTGGTAGTCTTGGTGTTCATAGCAG
TCAGTATCATAGCTACCCAT

Downstream 100 bases:

>100_bases
GGGAATAAATGAATAAAAAAGTAGTAATTACCTCTGCCCTTGCGGTTTGGTTTTCATTACTTCCCTTATGCCGGGCGCAG
TCTGCCTTGGCGGTACCCAA

Product: microcin-processing peptidase 1

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MTQKPSTRHSTPSAGSVSGLKSRTTRQPESGFSYHRDFFEELVDTALDHAKKLGASDAAAEASEGCGLSVSVRKGELENV
ERNRDKSLGITVYIGKKRGNAATSDFSKAAIQQTVKAAYDIARFTAEDETSGLPDDRDIAREYPALDLFHPWAITSEEAA
HLALQCEAAAMGTSRLITNSEGAGVSAQQSHFFSAHTKGFRGGYASSRHSISVAPIAGKGNQMQRDAWYSSMRDANELAR
PEAVGRYAAERALARLRSRKIPTTQCPVLFESPLAAGLLGGFVQAVSGGALYRKSTFLANSLGKAAFPRHIDIYEDPHLM
KGKGSAPFDEEGVKTSARRVVDAGRVEGYFLSTYSARKLGMRTTGNAGGSHNLTLTSRHTRTGDDLDAMLRKLGRGLFVT
ELMGQGVNYVTGDYSRGASGFWVENGRIAFPVEEITIAGNLKAMLMGIEAVGADMYTYGSKSVGSMLVNRMKVAGT

Sequences:

>Translated_476_residues
MTQKPSTRHSTPSAGSVSGLKSRTTRQPESGFSYHRDFFEELVDTALDHAKKLGASDAAAEASEGCGLSVSVRKGELENV
ERNRDKSLGITVYIGKKRGNAATSDFSKAAIQQTVKAAYDIARFTAEDETSGLPDDRDIAREYPALDLFHPWAITSEEAA
HLALQCEAAAMGTSRLITNSEGAGVSAQQSHFFSAHTKGFRGGYASSRHSISVAPIAGKGNQMQRDAWYSSMRDANELAR
PEAVGRYAAERALARLRSRKIPTTQCPVLFESPLAAGLLGGFVQAVSGGALYRKSTFLANSLGKAAFPRHIDIYEDPHLM
KGKGSAPFDEEGVKTSARRVVDAGRVEGYFLSTYSARKLGMRTTGNAGGSHNLTLTSRHTRTGDDLDAMLRKLGRGLFVT
ELMGQGVNYVTGDYSRGASGFWVENGRIAFPVEEITIAGNLKAMLMGIEAVGADMYTYGSKSVGSMLVNRMKVAGT
>Mature_475_residues
TQKPSTRHSTPSAGSVSGLKSRTTRQPESGFSYHRDFFEELVDTALDHAKKLGASDAAAEASEGCGLSVSVRKGELENVE
RNRDKSLGITVYIGKKRGNAATSDFSKAAIQQTVKAAYDIARFTAEDETSGLPDDRDIAREYPALDLFHPWAITSEEAAH
LALQCEAAAMGTSRLITNSEGAGVSAQQSHFFSAHTKGFRGGYASSRHSISVAPIAGKGNQMQRDAWYSSMRDANELARP
EAVGRYAAERALARLRSRKIPTTQCPVLFESPLAAGLLGGFVQAVSGGALYRKSTFLANSLGKAAFPRHIDIYEDPHLMK
GKGSAPFDEEGVKTSARRVVDAGRVEGYFLSTYSARKLGMRTTGNAGGSHNLTLTSRHTRTGDDLDAMLRKLGRGLFVTE
LMGQGVNYVTGDYSRGASGFWVENGRIAFPVEEITIAGNLKAMLMGIEAVGADMYTYGSKSVGSMLVNRMKVAGT

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=442, Percent_Identity=47.2850678733032, Blast_Score=391, Evalue=1e-110,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 51119; Mature: 50988

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQKPSTRHSTPSAGSVSGLKSRTTRQPESGFSYHRDFFEELVDTALDHAKKLGASDAAA
CCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
EASEGCGLSVSVRKGELENVERNRDKSLGITVYIGKKRGNAATSDFSKAAIQQTVKAAYD
HHHCCCCEEEEECCCCHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHH
IARFTAEDETSGLPDDRDIAREYPALDLFHPWAITSEEAAHLALQCEAAAMGTSRLITNS
HHHHCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCHHHHEEEEEEHHHHCCCHHEEECC
EGAGVSAQQSHFFSAHTKGFRGGYASSRHSISVAPIAGKGNQMQRDAWYSSMRDANELAR
CCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHC
PEAVGRYAAERALARLRSRKIPTTQCPVLFESPLAAGLLGGFVQAVSGGALYRKSTFLAN
HHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHH
SLGKAAFPRHIDIYEDPHLMKGKGSAPFDEEGVKTSARRVVDAGRVEGYFLSTYSARKLG
HHCHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCHHHHCC
MRTTGNAGGSHNLTLTSRHTRTGDDLDAMLRKLGRGLFVTELMGQGVNYVTGDYSRGASG
CCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHCCCEEHHHHHHCCCCEEECCCCCCCCC
FWVENGRIAFPVEEITIAGNLKAMLMGIEAVGADMYTYGSKSVGSMLVNRMKVAGT
EEEECCEEEECHHHEEECCCHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TQKPSTRHSTPSAGSVSGLKSRTTRQPESGFSYHRDFFEELVDTALDHAKKLGASDAAA
CCCCCCCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
EASEGCGLSVSVRKGELENVERNRDKSLGITVYIGKKRGNAATSDFSKAAIQQTVKAAYD
HHHCCCCEEEEECCCCHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHH
IARFTAEDETSGLPDDRDIAREYPALDLFHPWAITSEEAAHLALQCEAAAMGTSRLITNS
HHHHCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCHHHHEEEEEEHHHHCCCHHEEECC
EGAGVSAQQSHFFSAHTKGFRGGYASSRHSISVAPIAGKGNQMQRDAWYSSMRDANELAR
CCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHC
PEAVGRYAAERALARLRSRKIPTTQCPVLFESPLAAGLLGGFVQAVSGGALYRKSTFLAN
HHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHH
SLGKAAFPRHIDIYEDPHLMKGKGSAPFDEEGVKTSARRVVDAGRVEGYFLSTYSARKLG
HHCHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCHHHHCC
MRTTGNAGGSHNLTLTSRHTRTGDDLDAMLRKLGRGLFVTELMGQGVNYVTGDYSRGASG
CCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHCCCEEHHHHHHCCCCEEECCCCCCCCC
FWVENGRIAFPVEEITIAGNLKAMLMGIEAVGADMYTYGSKSVGSMLVNRMKVAGT
EEEECCEEEECHHHEEECCCHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]