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The map label for this gene is gdhA [H]

Identifier: 91789099

GI number: 91789099

Start: 3429060

End: 3430376

Strand: Direct

Name: gdhA [H]

Synonym: Bpro_3239

Alternate gene names: 91789099

Gene position: 3429060-3430376 (Clockwise)

Preceding gene: 91789098

Following gene: 91789124

Centisome position: 65.94

GC content: 62.87

Gene sequence:

>1317_bases
ATGTCACAAGCACCCATCCCCAGCGCAAGCGCCGCAGCAGGCACCCATCCGCTTCCTTCTTACCTGCAGGCCGACAAGCT
CGGGCCCTGGGGCAACTACCTGCAGCAGGTGGACCGCGTCATACCGCACCTGGGCAGCCTGGGTCGGTGGGCAGAAACGC
TCAAGCGGCCCAAGCGCATCCTGGTTGTCGATGTGCCCATCAACATGGACGACGGCACCATTGCGCACTTTGAAGGCTAC
AGGGTTCAGCACAACGTGTCGCGCGGCCCGGGCAAGGGCGGCGTGCGTTTTCACCAGGATGTGACGCTGTCGGAGGTGAT
GGCGCTGTCGGCCTGGATGTCCATCAAGAATGCTGCTGTGAATGTTCCTTTCGGGGGTGCCAAGGGCGGCATACGCGTGG
ACCCAAAAACAGTTTCCCAGGGCGAACTGGAACGCATCACGCGGCGCTACACGAGCGAGATCGGCATCATCATCGGCCCC
ACCAAGGACATCCCGGCGCCCGATGTGAATACCAACGAGCAGGTCATGGCGTGGATGATGGACACCTACTCGATGAACAC
GGGCTCCACGTCAACCGGGGTGGTCACTGGCAAACCGGTCGACCTGGGGGGCTCACTGGGTCGCCGGGATGCCACCGGGC
GCGGCGTTTTCACGGTGGGCGTGGAAGCGGCCAGGCACATTGACCTGGACATCAGCACGTCGCGGGTTGCGGTACAGGGT
TTCGGCAACGTGGGCGGCGTCGCCGGCAGGCTGTTCCATGAAACGGGGGCGCGTATCGTGGCCGTGCAGGATCATGGCGG
CACGATTTACCGCGAAGCCGGCCTGGACGTGCCAGCCCTGATCAGGCATGTGGCCGAGACCGGCAGCGTGGGAGGCTTCC
CGAACGCGGAAGTGATTGCCAATGAGCTGTTCTGGGAAGTCGACTGCGACATCATGATTCCGGCTGCGCTGGAAGAGCAG
ATCAATGCCGCCAACGCCGGCCGCATCAAGGCCCGCATGATCATCGAAGGCGCCAACGGCCCGACCACACCCGAGGCCGA
CGACATCCTGCAGGAACGCAACGTGCTGGTGCTGCCTGATGTGATCGCCAATGCGGGCGGCGTGACCGTCAGTTATTTTG
AATGGGTGCAGGACTTTTCCAGCTTTTTCTGGGATGAAGCGGAGATCAATGCACGCCTGGTCCGCATCATGAAAGAAGCT
TTCGCCGGCGTCTGGCAAGTCGCGCAGGACCACAAGGTGACGCTGCGCACCGCCACGTTCATCGTGGCTTGCAAACGCAT
CCTGCATACCCGCCAGCTGCGCGGCCTGTACCCCTGA

Upstream 100 bases:

>100_bases
CTATGCATTTTTTGCATGACGATGACCGAACAATGCCTTGGACAGTTTGACTCGCGGCTTCTAAGCTTGGCGACCTGAAT
GTTCCACAAGGAGATTTCGC

Downstream 100 bases:

>100_bases
GTCGCTGAGTCAAGAGGAGCCCATCGCGCCAAAGAGCTGAGTGCAATGAACGACTTTGGCGGCTCGACATTTCAAGCAAG
GGCCGCGGAACCGGCTTTGC

Product: Glu/Leu/Phe/Val dehydrogenase, C terminal

Products: NA

Alternate protein names: GDH [H]

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MSQAPIPSASAAAGTHPLPSYLQADKLGPWGNYLQQVDRVIPHLGSLGRWAETLKRPKRILVVDVPINMDDGTIAHFEGY
RVQHNVSRGPGKGGVRFHQDVTLSEVMALSAWMSIKNAAVNVPFGGAKGGIRVDPKTVSQGELERITRRYTSEIGIIIGP
TKDIPAPDVNTNEQVMAWMMDTYSMNTGSTSTGVVTGKPVDLGGSLGRRDATGRGVFTVGVEAARHIDLDISTSRVAVQG
FGNVGGVAGRLFHETGARIVAVQDHGGTIYREAGLDVPALIRHVAETGSVGGFPNAEVIANELFWEVDCDIMIPAALEEQ
INAANAGRIKARMIIEGANGPTTPEADDILQERNVLVLPDVIANAGGVTVSYFEWVQDFSSFFWDEAEINARLVRIMKEA
FAGVWQVAQDHKVTLRTATFIVACKRILHTRQLRGLYP

Sequences:

>Translated_438_residues
MSQAPIPSASAAAGTHPLPSYLQADKLGPWGNYLQQVDRVIPHLGSLGRWAETLKRPKRILVVDVPINMDDGTIAHFEGY
RVQHNVSRGPGKGGVRFHQDVTLSEVMALSAWMSIKNAAVNVPFGGAKGGIRVDPKTVSQGELERITRRYTSEIGIIIGP
TKDIPAPDVNTNEQVMAWMMDTYSMNTGSTSTGVVTGKPVDLGGSLGRRDATGRGVFTVGVEAARHIDLDISTSRVAVQG
FGNVGGVAGRLFHETGARIVAVQDHGGTIYREAGLDVPALIRHVAETGSVGGFPNAEVIANELFWEVDCDIMIPAALEEQ
INAANAGRIKARMIIEGANGPTTPEADDILQERNVLVLPDVIANAGGVTVSYFEWVQDFSSFFWDEAEINARLVRIMKEA
FAGVWQVAQDHKVTLRTATFIVACKRILHTRQLRGLYP
>Mature_437_residues
SQAPIPSASAAAGTHPLPSYLQADKLGPWGNYLQQVDRVIPHLGSLGRWAETLKRPKRILVVDVPINMDDGTIAHFEGYR
VQHNVSRGPGKGGVRFHQDVTLSEVMALSAWMSIKNAAVNVPFGGAKGGIRVDPKTVSQGELERITRRYTSEIGIIIGPT
KDIPAPDVNTNEQVMAWMMDTYSMNTGSTSTGVVTGKPVDLGGSLGRRDATGRGVFTVGVEAARHIDLDISTSRVAVQGF
GNVGGVAGRLFHETGARIVAVQDHGGTIYREAGLDVPALIRHVAETGSVGGFPNAEVIANELFWEVDCDIMIPAALEEQI
NAANAGRIKARMIIEGANGPTTPEADDILQERNVLVLPDVIANAGGVTVSYFEWVQDFSSFFWDEAEINARLVRIMKEAF
AGVWQVAQDHKVTLRTATFIVACKRILHTRQLRGLYP

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=368, Percent_Identity=44.5652173913043, Blast_Score=301, Evalue=5e-82,
Organism=Homo sapiens, GI4885281, Length=368, Percent_Identity=43.75, Blast_Score=295, Evalue=4e-80,
Organism=Escherichia coli, GI1788059, Length=359, Percent_Identity=33.4261838440111, Blast_Score=171, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17544676, Length=394, Percent_Identity=39.5939086294416, Blast_Score=269, Evalue=3e-72,
Organism=Saccharomyces cerevisiae, GI6319256, Length=393, Percent_Identity=29.7709923664122, Blast_Score=137, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6324951, Length=388, Percent_Identity=30.9278350515464, Blast_Score=133, Evalue=4e-32,
Organism=Drosophila melanogaster, GI45553475, Length=362, Percent_Identity=43.646408839779, Blast_Score=282, Evalue=3e-76,
Organism=Drosophila melanogaster, GI45549226, Length=361, Percent_Identity=43.4903047091413, Blast_Score=280, Evalue=1e-75,
Organism=Drosophila melanogaster, GI24649283, Length=331, Percent_Identity=43.202416918429, Blast_Score=266, Evalue=2e-71,
Organism=Drosophila melanogaster, GI24649460, Length=308, Percent_Identity=43.5064935064935, Blast_Score=234, Evalue=1e-61,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.3 [H]

Molecular weight: Translated: 47321; Mature: 47190

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQAPIPSASAAAGTHPLPSYLQADKLGPWGNYLQQVDRVIPHLGSLGRWAETLKRPKRI
CCCCCCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEE
LVVDVPINMDDGTIAHFEGYRVQHNVSRGPGKGGVRFHQDVTLSEVMALSAWMSIKNAAV
EEEEECCCCCCCCEEEEECEEEEECCCCCCCCCCCEEECCCCHHHHHHHHHHHHHCCCEE
NVPFGGAKGGIRVDPKTVSQGELERITRRYTSEIGIIIGPTKDIPAPDVNTNEQVMAWMM
ECCCCCCCCCEEECCCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCHHHHHHHH
DTYSMNTGSTSTGVVTGKPVDLGGSLGRRDATGRGVFTVGVEAARHIDLDISTSRVAVQG
HHHCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCEEEEECCCCEEEEEECCCCEEEEEC
FGNVGGVAGRLFHETGARIVAVQDHGGTIYREAGLDVPALIRHVAETGSVGGFPNAEVIA
CCCCCHHHHHHHHHCCCEEEEEECCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCHHHHH
NELFWEVDCDIMIPAALEEQINAANAGRIKARMIIEGANGPTTPEADDILQERNVLVLPD
HHHEEEECCCEECCHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHCCEEEECH
VIANAGGVTVSYFEWVQDFSSFFWDEAEINARLVRIMKEAFAGVWQVAQDHKVTLRTATF
HHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHH
IVACKRILHTRQLRGLYP
HHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SQAPIPSASAAAGTHPLPSYLQADKLGPWGNYLQQVDRVIPHLGSLGRWAETLKRPKRI
CCCCCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEE
LVVDVPINMDDGTIAHFEGYRVQHNVSRGPGKGGVRFHQDVTLSEVMALSAWMSIKNAAV
EEEEECCCCCCCCEEEEECEEEEECCCCCCCCCCCEEECCCCHHHHHHHHHHHHHCCCEE
NVPFGGAKGGIRVDPKTVSQGELERITRRYTSEIGIIIGPTKDIPAPDVNTNEQVMAWMM
ECCCCCCCCCEEECCCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCHHHHHHHH
DTYSMNTGSTSTGVVTGKPVDLGGSLGRRDATGRGVFTVGVEAARHIDLDISTSRVAVQG
HHHCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCEEEEECCCCEEEEEECCCCEEEEEC
FGNVGGVAGRLFHETGARIVAVQDHGGTIYREAGLDVPALIRHVAETGSVGGFPNAEVIA
CCCCCHHHHHHHHHCCCEEEEEECCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCHHHHH
NELFWEVDCDIMIPAALEEQINAANAGRIKARMIIEGANGPTTPEADDILQERNVLVLPD
HHHEEEECCCEECCHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHCCEEEECH
VIANAGGVTVSYFEWVQDFSSFFWDEAEINARLVRIMKEAFAGVWQVAQDHKVTLRTATF
HHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHH
IVACKRILHTRQLRGLYP
HHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9680336; 10360571; 9135121; 9654452; 10366510 [H]