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The map label for this gene is eno [H]

Identifier: 91789044

GI number: 91789044

Start: 3367916

End: 3369199

Strand: Direct

Name: eno [H]

Synonym: Bpro_3184

Alternate gene names: 91789044

Gene position: 3367916-3369199 (Clockwise)

Preceding gene: 91789043

Following gene: 91789045

Centisome position: 64.76

GC content: 61.14

Gene sequence:

>1284_bases
ATGAGCGCAATTGTTGATATCGTTGGCCGTGAAATTCTCGACAGCCGTGGCAATCCCACCGTTGAATGCGACGTGCTGCT
GGAGTCCGGCGTGATGGGCCGCGCCGCAGTCCCCTCGGGCGCCTCCACCGGTTCGCGCGAAGCCATCGAGCTGCGCGACG
GCGACAAATCGCGCTACCTTGGCAAGGGCGTGCTCAAGGCCGTCGAGCACATCAACACTGAAATCTCCGAAGCCGTGCTC
GGCCTGGATGCCAGCGAGCAGGCCTTTCTCGACCGTACGCTGATTGACCTGGACGGAACGGACAACAAGTCCCGCCTGGG
TGCCAATGCCACGCTGGCCGTCAGCATGGCAGTCGCGCGTGCGGCGGCTGAAGAAGCTGGCCTGCCGCTGTACCGCTACT
TCGGCGGCAGCGGCGCCATGCAAATGCCCGTTCCCATGATGAACGTGGTCAACGGCGGCGCGCACGCCAACAACAACCTT
GACTTGCAGGAGCTGATGATCATCCCCATCGGCGCACCGAGCTTTCGCGAAGCCGTGCGTTACGGTGCCGAGGTGTTTCA
TGCGCTCAAGAAAATCCTGCACGACAAGGGCATGAGCGTGGCCGTTGGTGACGAGGGCGGCTTTGCGCCGAATGTGCCCA
GCCACGAAGCGGCCATCCAGATGATCCTCGAGGCTATCGACAAGGCCGGCTATGTCGCGGGTGAACAAATTGCGCTGGGC
CTGGATTGCGCGGCCTCCGAGTTTTACAAGGATGGCAAGTACGTGCTGGCCGGCGAAGGCCTGAGCCTGGACGCCACCGA
ATGGACCAACATCCTGGCGACCTGGGTCGACAAATACCCCATCATCAGCATTGAGGACGGCATGGCCGAAGGCGACTGGG
ATGGCTGGAAAATACTGACCGAACGCCTGGGAAAACAGGTGCAACTGGTCGGCGACGACCTGTTTGTCACCAACACCAAG
ATTCTCAAGGAAGGCATCGACAAGCACATCGCCAACTCGATCCTGATCAAGATCAACCAGATTGGCACGCTGACGGAAAC
CTTCGCTGCGATTGAAATGGCCAAGCGCGCCGGCTACACCGCCGTCATCAGCCACCGCTCTGGCGAAACCGAAGACTCCA
CCATTGCCGACATCGCGGTTGGCACCAATGCCGGCCAGATCAAGACCGGTTCGCTCTCCCGCTCGGACCGCATGGCCAAG
TACAACCAGCTGCTGCGCATCGAGGAAGACCTGGGTGACATCGCGACGTATCCAGGCCGCGCAGCGTTCTACAACCTGCG
TTGA

Upstream 100 bases:

>100_bases
GTCAGTCGCGTCAGGGCATCGCCGTCATGCGCATGGTGCTGGTTGAAGGTGTGTAGTTTCAGTCTTCTGATTTTTGTTTT
CTGATTTTGAAAGAACATAA

Downstream 100 bases:

>100_bases
CCTGACCACATCCAATTCCACGGCTTGTGGCTAAACCTTGGCTAATCGCTTCATCCCTGCCATCCTGATTGCGTTGCTGG
TGTTGTTTCATGCGCAACTC

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MSAIVDIVGREILDSRGNPTVECDVLLESGVMGRAAVPSGASTGSREAIELRDGDKSRYLGKGVLKAVEHINTEISEAVL
GLDASEQAFLDRTLIDLDGTDNKSRLGANATLAVSMAVARAAAEEAGLPLYRYFGGSGAMQMPVPMMNVVNGGAHANNNL
DLQELMIIPIGAPSFREAVRYGAEVFHALKKILHDKGMSVAVGDEGGFAPNVPSHEAAIQMILEAIDKAGYVAGEQIALG
LDCAASEFYKDGKYVLAGEGLSLDATEWTNILATWVDKYPIISIEDGMAEGDWDGWKILTERLGKQVQLVGDDLFVTNTK
ILKEGIDKHIANSILIKINQIGTLTETFAAIEMAKRAGYTAVISHRSGETEDSTIADIAVGTNAGQIKTGSLSRSDRMAK
YNQLLRIEEDLGDIATYPGRAAFYNLR

Sequences:

>Translated_427_residues
MSAIVDIVGREILDSRGNPTVECDVLLESGVMGRAAVPSGASTGSREAIELRDGDKSRYLGKGVLKAVEHINTEISEAVL
GLDASEQAFLDRTLIDLDGTDNKSRLGANATLAVSMAVARAAAEEAGLPLYRYFGGSGAMQMPVPMMNVVNGGAHANNNL
DLQELMIIPIGAPSFREAVRYGAEVFHALKKILHDKGMSVAVGDEGGFAPNVPSHEAAIQMILEAIDKAGYVAGEQIALG
LDCAASEFYKDGKYVLAGEGLSLDATEWTNILATWVDKYPIISIEDGMAEGDWDGWKILTERLGKQVQLVGDDLFVTNTK
ILKEGIDKHIANSILIKINQIGTLTETFAAIEMAKRAGYTAVISHRSGETEDSTIADIAVGTNAGQIKTGSLSRSDRMAK
YNQLLRIEEDLGDIATYPGRAAFYNLR
>Mature_426_residues
SAIVDIVGREILDSRGNPTVECDVLLESGVMGRAAVPSGASTGSREAIELRDGDKSRYLGKGVLKAVEHINTEISEAVLG
LDASEQAFLDRTLIDLDGTDNKSRLGANATLAVSMAVARAAAEEAGLPLYRYFGGSGAMQMPVPMMNVVNGGAHANNNLD
LQELMIIPIGAPSFREAVRYGAEVFHALKKILHDKGMSVAVGDEGGFAPNVPSHEAAIQMILEAIDKAGYVAGEQIALGL
DCAASEFYKDGKYVLAGEGLSLDATEWTNILATWVDKYPIISIEDGMAEGDWDGWKILTERLGKQVQLVGDDLFVTNTKI
LKEGIDKHIANSILIKINQIGTLTETFAAIEMAKRAGYTAVISHRSGETEDSTIADIAVGTNAGQIKTGSLSRSDRMAKY
NQLLRIEEDLGDIATYPGRAAFYNLR

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI5803011, Length=438, Percent_Identity=51.5981735159817, Blast_Score=431, Evalue=1e-121,
Organism=Homo sapiens, GI4503571, Length=430, Percent_Identity=48.8372093023256, Blast_Score=419, Evalue=1e-117,
Organism=Homo sapiens, GI301897477, Length=430, Percent_Identity=50.2325581395349, Blast_Score=412, Evalue=1e-115,
Organism=Homo sapiens, GI301897469, Length=430, Percent_Identity=50.2325581395349, Blast_Score=412, Evalue=1e-115,
Organism=Homo sapiens, GI301897479, Length=428, Percent_Identity=45.3271028037383, Blast_Score=355, Evalue=4e-98,
Organism=Homo sapiens, GI169201331, Length=336, Percent_Identity=27.3809523809524, Blast_Score=105, Evalue=9e-23,
Organism=Homo sapiens, GI169201757, Length=336, Percent_Identity=27.3809523809524, Blast_Score=105, Evalue=9e-23,
Organism=Homo sapiens, GI239744207, Length=336, Percent_Identity=27.3809523809524, Blast_Score=105, Evalue=9e-23,
Organism=Escherichia coli, GI1789141, Length=425, Percent_Identity=65.8823529411765, Blast_Score=556, Evalue=1e-160,
Organism=Caenorhabditis elegans, GI71995829, Length=437, Percent_Identity=51.0297482837529, Blast_Score=424, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17536383, Length=437, Percent_Identity=51.0297482837529, Blast_Score=424, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI32563855, Length=238, Percent_Identity=42.436974789916, Blast_Score=187, Evalue=8e-48,
Organism=Saccharomyces cerevisiae, GI6321693, Length=432, Percent_Identity=48.6111111111111, Blast_Score=386, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6324974, Length=433, Percent_Identity=49.1916859122402, Blast_Score=385, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6324969, Length=433, Percent_Identity=49.1916859122402, Blast_Score=385, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6323985, Length=433, Percent_Identity=48.9607390300231, Blast_Score=384, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6321968, Length=432, Percent_Identity=47.9166666666667, Blast_Score=362, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580918, Length=428, Percent_Identity=49.7663551401869, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24580916, Length=428, Percent_Identity=49.7663551401869, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24580920, Length=428, Percent_Identity=49.7663551401869, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24580914, Length=428, Percent_Identity=49.7663551401869, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI281360527, Length=428, Percent_Identity=49.7663551401869, Blast_Score=394, Evalue=1e-110,
Organism=Drosophila melanogaster, GI17137654, Length=428, Percent_Identity=49.7663551401869, Blast_Score=394, Evalue=1e-110,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 45678; Mature: 45547

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAIVDIVGREILDSRGNPTVECDVLLESGVMGRAAVPSGASTGSREAIELRDGDKSRYL
CCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHH
GKGVLKAVEHINTEISEAVLGLDASEQAFLDRTLIDLDGTDNKSRLGANATLAVSMAVAR
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHEEEECCCCCCHHHCCCCHHHHHHHHHHH
AAAEEAGLPLYRYFGGSGAMQMPVPMMNVVNGGAHANNNLDLQELMIIPIGAPSFREAVR
HHHHHCCCCEEEEECCCCCEECCCHHHHHHCCCCCCCCCCCHHHEEEEECCCHHHHHHHH
YGAEVFHALKKILHDKGMSVAVGDEGGFAPNVPSHEAAIQMILEAIDKAGYVAGEQIALG
HHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEE
LDCAASEFYKDGKYVLAGEGLSLDATEWTNILATWVDKYPIISIEDGMAEGDWDGWKILT
CHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHH
ERLGKQVQLVGDDLFVTNTKILKEGIDKHIANSILIKINQIGTLTETFAAIEMAKRAGYT
HHCCCEEEEECCCEEEEHHHHHHHHHHHHHHCEEEEEEECCCCHHHHHHHHHHHHHCCCE
AVISHRSGETEDSTIADIAVGTNAGQIKTGSLSRSDRMAKYNQLLRIEEDLGDIATYPGR
EEEECCCCCCCCCEEEEEEECCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
AAFYNLR
CEEECCC
>Mature Secondary Structure 
SAIVDIVGREILDSRGNPTVECDVLLESGVMGRAAVPSGASTGSREAIELRDGDKSRYL
CHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHH
GKGVLKAVEHINTEISEAVLGLDASEQAFLDRTLIDLDGTDNKSRLGANATLAVSMAVAR
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHEEEECCCCCCHHHCCCCHHHHHHHHHHH
AAAEEAGLPLYRYFGGSGAMQMPVPMMNVVNGGAHANNNLDLQELMIIPIGAPSFREAVR
HHHHHCCCCEEEEECCCCCEECCCHHHHHHCCCCCCCCCCCHHHEEEEECCCHHHHHHHH
YGAEVFHALKKILHDKGMSVAVGDEGGFAPNVPSHEAAIQMILEAIDKAGYVAGEQIALG
HHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEE
LDCAASEFYKDGKYVLAGEGLSLDATEWTNILATWVDKYPIISIEDGMAEGDWDGWKILT
CHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHH
ERLGKQVQLVGDDLFVTNTKILKEGIDKHIANSILIKINQIGTLTETFAAIEMAKRAGYT
HHCCCEEEEECCCEEEEHHHHHHHHHHHHHHCEEEEEEECCCCHHHHHHHHHHHHHCCCE
AVISHRSGETEDSTIADIAVGTNAGQIKTGSLSRSDRMAKYNQLLRIEEDLGDIATYPGR
EEEECCCCCCCCCEEEEEEECCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
AAFYNLR
CEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA