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The map label for this gene is rpoD [H]

Identifier: 91788957

GI number: 91788957

Start: 3267503

End: 3269857

Strand: Direct

Name: rpoD [H]

Synonym: Bpro_3097

Alternate gene names: 91788957

Gene position: 3267503-3269857 (Clockwise)

Preceding gene: 91788956

Following gene: 91788959

Centisome position: 62.83

GC content: 59.24

Gene sequence:

>2355_bases
ATGCCGACAGCCAAAACGGCAGCGAAACCCGCTGCCAAACCGGCCGCAAGGCATGGCCCCCAGAACGTCAAGGTTGAAAA
ACCCGGAAAACCCGCCATATCCGTTAAAGCCAGTAGCCCGAATGCCCCGGTGAGCAAAAAGCCCCTGAAATCCGCGAAAC
CTGATACGTCCCTGAAGAAAGTCCCTGCCGTGCCGACAAAGTCCAGCCCCGAATCCAAGCCTGTTGCAGCCGTTGCCGAC
GTGCCCGTGAAGAAAAAACCGGGTCGCCCGCCGAAAAACCCTGCAGCAGCCGATAGCGCACCTGCAAGCACCGGCGCCAA
GCGCGGCCGCAAGCCCAAGAACGCGGGCGCAGCCAAGGTCGGGGAAGACCTCGACCTGTCCGACATTGAAGAGGATCTGG
TCGGCGAACCCGTTGCGGAGACCACCGAAAAGGTCAAACCGCTGCGCATGAAGATCAGCAAGGCCAAAGAACGTGCCTTG
ATGAAGGAATTTGGCCTGGACGAGACGGTGTTGTCGGAAGAGGACATGCTCAAGCGCCGCATGCGCCTGAAAACCCTGAT
CAAGCTCGGCAAAACCCGCGGCTACCTCACGCACGGCGAAATTTCCGATCACCTGCCCGACAAGCTGGTCGACGCGGAAA
CGCTGGAAGTCGTCGTCAACATGCTCAACGACATGGGCGTGGCCGTGTACGAGCAGGCGCCGGACGCCGAAACCCTGCTG
CTGAACAACACCGGGCCGACCGTGGCCACGGAAGAAGAAGCCGAAGAAGAAGCCGAAGCCGCCCTGTCCACCGTGGACAG
CGAATTCGGCCGCACGACCGACCCTGTGCGCATGTACATGCGTGAAATGGGCACGGTCGAGCTGCTGACCCGCGAAGGCG
AAATCGAGATTGCCAAGCGCATCGAGGGCGGCCTCATGAAAATGATGGAAGCCATCTCGGAGAGCCCCGCCACCATCGCA
GAAATCATGCGGCTGGGCGAGGAAATCCGCGAAGGCAAGATCGTGATCTCGACCGTGGTGGACGGTTTCTCCAACCCCAA
CGAAGCCGATGACTATGTGGCCGAAGAGGACTTTGACGAGTTCGACGAGGAAGACGACGACGATGGCAAGGGCGGCTCCA
AGGCCCTGACCAAGAAACTCGAAGAGCTCAAAAAAGAAGCCCTCAGCCGCTTTGACAAAATTGCCAGCCTGTTCGAGAAG
GTGCACAAGACCTACGACAAGGAAGGCTATGGCACCCCGACTTATGTGAAGGCGCAAAAGGCCTTGAGCGACGAGCTCAT
GACCGTGCGCTTTACCGCCAAGACCATTGAAAAGCTCTGCGACATGCTGCGCGGCCAGGTGCTGGACGTGCGCAAGAAAG
AGCGCGAGCTGCGCCGCATCATCGTCGAGAAGTGCGGTATGCCCCAGGAAACCTTCATCAAGGATTTCCCGCCCAATCTG
CTCAACCTCAAGTGGGTTGAAAAACAGGTCGCGTCGGGCAAGCCCTGGTCCACCGTGATGGCGCGAAATATTCCGCCGAT
TCAGGAACTGCAGACCAAGCTGGCCGAATTGCAGGCCCGCGTGGTGGTGCCGCTGGGTCAGCTCAAGGACATCAACAAGC
GCATGAACGAAGGCGAGTCCAACTCGCGCGATGCCAAGAAAGAGATGATCGAGGCCAATTTGCGCCTGGTGATCTCGATT
GCCAAAAAATACACCAACCGCGGCCTGCAGTTCCTGGACCTGATCCAGGAAGGCAACATCGGTTTGATGAAAGCCGTCGA
CAAGTTCGAATACCGCCGCGGCTACAAATTCTCGACCTATGCCACCTGGTGGATTCGCCAGGCGATCACGCGCTCCATCG
CGGACCAGGCCCGTACCATCCGCATCCCGGTCCACATGATCGAGACCATCAACAAGATGAACCGCCTGTCGCGCCAGCAT
CTGCAGGAATTCGGCTTTGAGCCCGACGCCAGCATCCTGGCCGCCAAGATGGAGATTCCTGAAGACAAGATCCGCAAGAT
CATGAAGATCGCCAAGGAGCCGATCTCGATGGAAACGCCGATCGGTGACGATGATGATTCGCACCTCGGCGACTTCATCG
AAGACAGCGCCAACACCGCACCGCTGGAGGCCGCAATGCAGGCCGGCCTGCGCGATGTGGTGAAAGACATCCTCGACAGC
CTGACACCGCGCGAAGCCAAGGTGCTGCGCATGCGTTTCGGCATCGAGATGTCGACCGACCACACGCTGGAAGAAGTTGG
CAAGCAGTTTGACGTGACGCGCGAGCGCATCCGCCAGATCGAAGCCAAGGCGCTGCGCAAGCTCAAGCACCCGAGCCGCA
GCGACAAGCTGCGCAGCTTTATCGATACGCTGTAA

Upstream 100 bases:

>100_bases
ATAACAATTCCCGACATCTGGAGGTCTCATGCCTAGTGCCACGCCCGGCAAGTCCAAGAAGCCAAAAAAGCCGCAAACCA
CGCAGTCCAAAGCTGCCGTG

Downstream 100 bases:

>100_bases
AAAGCGGCTCGACGGGCCACAGCGGCCGGCACCTGTCAAAGGTGCCGGCCGCTTTTTTTTTCGATTGGCGCGAACTCGAT
ATTCAGTCGGACAGTGCGCG

Product: sigma 70 (RpoD)

Products: NA

Alternate protein names: Sigma-70 [H]

Number of amino acids: Translated: 784; Mature: 783

Protein sequence:

>784_residues
MPTAKTAAKPAAKPAARHGPQNVKVEKPGKPAISVKASSPNAPVSKKPLKSAKPDTSLKKVPAVPTKSSPESKPVAAVAD
VPVKKKPGRPPKNPAAADSAPASTGAKRGRKPKNAGAAKVGEDLDLSDIEEDLVGEPVAETTEKVKPLRMKISKAKERAL
MKEFGLDETVLSEEDMLKRRMRLKTLIKLGKTRGYLTHGEISDHLPDKLVDAETLEVVVNMLNDMGVAVYEQAPDAETLL
LNNTGPTVATEEEAEEEAEAALSTVDSEFGRTTDPVRMYMREMGTVELLTREGEIEIAKRIEGGLMKMMEAISESPATIA
EIMRLGEEIREGKIVISTVVDGFSNPNEADDYVAEEDFDEFDEEDDDDGKGGSKALTKKLEELKKEALSRFDKIASLFEK
VHKTYDKEGYGTPTYVKAQKALSDELMTVRFTAKTIEKLCDMLRGQVLDVRKKERELRRIIVEKCGMPQETFIKDFPPNL
LNLKWVEKQVASGKPWSTVMARNIPPIQELQTKLAELQARVVVPLGQLKDINKRMNEGESNSRDAKKEMIEANLRLVISI
AKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRLSRQH
LQEFGFEPDASILAAKMEIPEDKIRKIMKIAKEPISMETPIGDDDDSHLGDFIEDSANTAPLEAAMQAGLRDVVKDILDS
LTPREAKVLRMRFGIEMSTDHTLEEVGKQFDVTRERIRQIEAKALRKLKHPSRSDKLRSFIDTL

Sequences:

>Translated_784_residues
MPTAKTAAKPAAKPAARHGPQNVKVEKPGKPAISVKASSPNAPVSKKPLKSAKPDTSLKKVPAVPTKSSPESKPVAAVAD
VPVKKKPGRPPKNPAAADSAPASTGAKRGRKPKNAGAAKVGEDLDLSDIEEDLVGEPVAETTEKVKPLRMKISKAKERAL
MKEFGLDETVLSEEDMLKRRMRLKTLIKLGKTRGYLTHGEISDHLPDKLVDAETLEVVVNMLNDMGVAVYEQAPDAETLL
LNNTGPTVATEEEAEEEAEAALSTVDSEFGRTTDPVRMYMREMGTVELLTREGEIEIAKRIEGGLMKMMEAISESPATIA
EIMRLGEEIREGKIVISTVVDGFSNPNEADDYVAEEDFDEFDEEDDDDGKGGSKALTKKLEELKKEALSRFDKIASLFEK
VHKTYDKEGYGTPTYVKAQKALSDELMTVRFTAKTIEKLCDMLRGQVLDVRKKERELRRIIVEKCGMPQETFIKDFPPNL
LNLKWVEKQVASGKPWSTVMARNIPPIQELQTKLAELQARVVVPLGQLKDINKRMNEGESNSRDAKKEMIEANLRLVISI
AKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRLSRQH
LQEFGFEPDASILAAKMEIPEDKIRKIMKIAKEPISMETPIGDDDDSHLGDFIEDSANTAPLEAAMQAGLRDVVKDILDS
LTPREAKVLRMRFGIEMSTDHTLEEVGKQFDVTRERIRQIEAKALRKLKHPSRSDKLRSFIDTL
>Mature_783_residues
PTAKTAAKPAAKPAARHGPQNVKVEKPGKPAISVKASSPNAPVSKKPLKSAKPDTSLKKVPAVPTKSSPESKPVAAVADV
PVKKKPGRPPKNPAAADSAPASTGAKRGRKPKNAGAAKVGEDLDLSDIEEDLVGEPVAETTEKVKPLRMKISKAKERALM
KEFGLDETVLSEEDMLKRRMRLKTLIKLGKTRGYLTHGEISDHLPDKLVDAETLEVVVNMLNDMGVAVYEQAPDAETLLL
NNTGPTVATEEEAEEEAEAALSTVDSEFGRTTDPVRMYMREMGTVELLTREGEIEIAKRIEGGLMKMMEAISESPATIAE
IMRLGEEIREGKIVISTVVDGFSNPNEADDYVAEEDFDEFDEEDDDDGKGGSKALTKKLEELKKEALSRFDKIASLFEKV
HKTYDKEGYGTPTYVKAQKALSDELMTVRFTAKTIEKLCDMLRGQVLDVRKKERELRRIIVEKCGMPQETFIKDFPPNLL
NLKWVEKQVASGKPWSTVMARNIPPIQELQTKLAELQARVVVPLGQLKDINKRMNEGESNSRDAKKEMIEANLRLVISIA
KKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRLSRQHL
QEFGFEPDASILAAKMEIPEDKIRKIMKIAKEPISMETPIGDDDDSHLGDFIEDSANTAPLEAAMQAGLRDVVKDILDSL
TPREAKVLRMRFGIEMSTDHTLEEVGKQFDVTRERIRQIEAKALRKLKHPSRSDKLRSFIDTL

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=610, Percent_Identity=53.1147540983606, Blast_Score=606, Evalue=1e-174,
Organism=Escherichia coli, GI1789098, Length=225, Percent_Identity=40.4444444444444, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI1789871, Length=242, Percent_Identity=27.6859504132231, Blast_Score=86, Evalue=8e-18,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007631
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04546 Sigma70_ner; PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 87489; Mature: 87357

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2 ; PS00867 CPSASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTAKTAAKPAAKPAARHGPQNVKVEKPGKPAISVKASSPNAPVSKKPLKSAKPDTSLKK
CCCCCCCCCCCCCCHHHCCCCCCEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHH
VPAVPTKSSPESKPVAAVADVPVKKKPGRPPKNPAAADSAPASTGAKRGRKPKNAGAAKV
CCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCHHC
GEDLDLSDIEEDLVGEPVAETTEKVKPLRMKISKAKERALMKEFGLDETVLSEEDMLKRR
CCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHH
MRLKTLIKLGKTRGYLTHGEISDHLPDKLVDAETLEVVVNMLNDMGVAVYEQAPDAETLL
HHHHHHHHHHHCCCCEECCCHHHCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCEEE
LNNTGPTVATEEEAEEEAEAALSTVDSEFGRTTDPVRMYMREMGTVELLTREGEIEIAKR
EECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEECCCCHHHHHH
IEGGLMKMMEAISESPATIAEIMRLGEEIREGKIVISTVVDGFSNPNEADDYVAEEDFDE
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEHHHHHHCCCCCCCHHHHHHHCCHHH
FDEEDDDDGKGGSKALTKKLEELKKEALSRFDKIASLFEKVHKTYDKEGYGTPTYVKAQK
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
ALSDELMTVRFTAKTIEKLCDMLRGQVLDVRKKERELRRIIVEKCGMPQETFIKDFPPNL
HHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCC
LNLKWVEKQVASGKPWSTVMARNIPPIQELQTKLAELQARVVVPLGQLKDINKRMNEGES
CHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCC
NSRDAKKEMIEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTY
CCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHH
ATWWIRQAITRSIADQARTIRIPVHMIETINKMNRLSRQHLQEFGFEPDASILAAKMEIP
HHHHHHHHHHHHHHHHHHEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCC
EDKIRKIMKIAKEPISMETPIGDDDDSHLGDFIEDSANTAPLEAAMQAGLRDVVKDILDS
HHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
LTPREAKVLRMRFGIEMSTDHTLEEVGKQFDVTRERIRQIEAKALRKLKHPSRSDKLRSF
CCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
IDTL
HHCC
>Mature Secondary Structure 
PTAKTAAKPAAKPAARHGPQNVKVEKPGKPAISVKASSPNAPVSKKPLKSAKPDTSLKK
CCCCCCCCCCCCCHHHCCCCCCEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHH
VPAVPTKSSPESKPVAAVADVPVKKKPGRPPKNPAAADSAPASTGAKRGRKPKNAGAAKV
CCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCHHC
GEDLDLSDIEEDLVGEPVAETTEKVKPLRMKISKAKERALMKEFGLDETVLSEEDMLKRR
CCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHH
MRLKTLIKLGKTRGYLTHGEISDHLPDKLVDAETLEVVVNMLNDMGVAVYEQAPDAETLL
HHHHHHHHHHHCCCCEECCCHHHCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCEEE
LNNTGPTVATEEEAEEEAEAALSTVDSEFGRTTDPVRMYMREMGTVELLTREGEIEIAKR
EECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEECCCCHHHHHH
IEGGLMKMMEAISESPATIAEIMRLGEEIREGKIVISTVVDGFSNPNEADDYVAEEDFDE
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEHHHHHHCCCCCCCHHHHHHHCCHHH
FDEEDDDDGKGGSKALTKKLEELKKEALSRFDKIASLFEKVHKTYDKEGYGTPTYVKAQK
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
ALSDELMTVRFTAKTIEKLCDMLRGQVLDVRKKERELRRIIVEKCGMPQETFIKDFPPNL
HHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCC
LNLKWVEKQVASGKPWSTVMARNIPPIQELQTKLAELQARVVVPLGQLKDINKRMNEGES
CHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCC
NSRDAKKEMIEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTY
CCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHH
ATWWIRQAITRSIADQARTIRIPVHMIETINKMNRLSRQHLQEFGFEPDASILAAKMEIP
HHHHHHHHHHHHHHHHHHEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCC
EDKIRKIMKIAKEPISMETPIGDDDDSHLGDFIEDSANTAPLEAAMQAGLRDVVKDILDS
HHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
LTPREAKVLRMRFGIEMSTDHTLEEVGKQFDVTRERIRQIEAKALRKLKHPSRSDKLRSF
CCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
IDTL
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA