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The map label for this gene is dnaG [H]

Identifier: 91788956

GI number: 91788956

Start: 3265102

End: 3267084

Strand: Direct

Name: dnaG [H]

Synonym: Bpro_3096

Alternate gene names: 91788956

Gene position: 3265102-3267084 (Clockwise)

Preceding gene: 91788954

Following gene: 91788957

Centisome position: 62.79

GC content: 64.09

Gene sequence:

>1983_bases
GTGGCCATTCCCCAATCATTTATCCAGGAGCTGCTCGCGCGGGCTGACGTGGTGGACATTGTGGGTCGCCACGTCCAGCT
GAAAAAGGGCGGCGCCAACTTCATGGGCCTGTGCCCGTTTCACGGCGAAAAGTCGCCCTCCTTCAGCGTCAGCCCGTCCA
AGCAGTTCTACCACTGCTTCGGCTGCGGCAAAAACGGCAATGTCATCAGCTTCCTGATGGAGCATGCCGGCATGAGCTTC
ATCGAAGCCGTGAAGGACCTGGCCCAGCAATACGGCCTGCAGGTGCCCGAAGACGACGTGTCGCCCCAGGACAGGGCCAA
GGCGGCACAGATGCGCGAACAGCAGGCCACCCTGACCAGTGTGCTGGAAAAAGCCGCCATGGCCTACATCAAGCACCTGC
GCGGCTCCGAGCGGGCCATCGAGTACTTCAAGGGCCGCGGCCTGTCGGGCGAGATCGCCAGGACTTTTGGGCTTGGTTAC
GCACCCGAGGGCTGGCGCAGCCTGGCCAGCGTATTTCCCGACTACAACGACCCGCTGCTGGTGGAAAGCGGCCTGGTGAT
CACCGGCGAGGCGGGCGAGGAAAACGCGGGCGGTGAAGCCAAGCGCTACGACCGCTTCCGCGACCGGGTCATGTTTCCCA
TCCGCAACGTCAAGGGCGAATGCATTGGCTTTGGCGGCCGCGTGCTGGGTGACGAAAAGCCCAAGTACCTGAACTCACCC
GAAACCCCGGTGTTCAGCAAGGGGCGCGAGCTCTACGGCCTGTTTGAAGCCCGCACGGCCTTGCGCGAGCACGGCTACGC
ACTGGTCACCGAAGGCTACATGGACGTGGTCGCGCTGGCCCAGCTGGGTTTTGCCAATGCCGTGGCCACCCTGGGCACGG
CCTGTACCGCCGACCATGTGCAAAAGCTGTTTCGCTTCACCGACTCGGTCGTCTTCAGCTTCGATGGTGACGGCGCCGGT
CGCCGCGCCGCGCGCAAGGCACTGGATGCCGCCCTGCCCTTTGCCACCGACGTGCGTACCGTCAAGTTTTTGTTTTTACC
GTCCGAGCACGACCCCGACAGTTACATCCGCGAACACGGCAAGGAAGGTTTTGCGGCTTACGTCAGCAAGGCTGTGCCGC
TGAGCCGCTTCCTGCTCGAAGCCGCTGCCGAGGGCTGCGACCTGGACACGGCGGAAGGCCGCGCCCACATGGCCAGCAAC
GCCCGCCCGCTGTGGAGCCTGTTGCCCGACGGTGCCCTGAAGCGCCAGCTGCTGGCCGAAATCGCCGACCAGGTGATGAT
TGACAGCCGCGAGTTGCTGCAGCTTTGGCAGCCCGCCTCCGCAGGTTACAAAAACAGCTATAAAAACAATAGCAGCAAGC
GCCTGCCCAATAAAGACCACAGGCCGGATTTACCCTCGGATTTCGGCAAAAACCACGACGTCCCCGACTATGCAGGCTAC
GTACCGGACCTACGCGAGGAGGCCGACTATGCCTCTTCCGAGCCGTATTTCCCGCCGCCCGCCATGTCAGCGCAGCGCCC
CGGGCGGTCGTCGGGTGGCGCCAGCCGGGCTCCGCGCCGCATCGCGGGCCGCATCCTGCCGGCCAGCCGTGAAGACCGCG
TCCTGCGGCTGCTGCTGACCGAGCCGCAGAGCTGGGACCAGCTCAGCACCGAAGAGCATCACCTGCTGCTGGCGTTGCCG
GCGCCGCACGGACCGCTGTTTGTCTGGCTGGAAAGCCAGCTGCATGAGCACGGCCCGCAACCCTGGGCCGCCCTGCGCGA
GGGCCTGCGCGAGCACCCCCACGAAAAGCACGCCATCGCGCAAATCGCGCAAATACCGGAAGGTATCGAGTGCGACTGGG
ATGAGGTCAGGAGCATCCTGGACCAGCTTGTCAGGCTCGAACGCCAGCGCGAAATGGACGAATTGGTGCCGCTGGTCACA
AAAGATCCCGCAGCCCGTCAGCGCTACCAGGAACTGCTGGAAGCCAACCTCAAAAAAAGCTGA

Upstream 100 bases:

>100_bases
TTCCGACCGGCCGCCCCTGGGGAAAAACGCCCCTCTCGGGGGCAGCGCAGTACGCGAAGCGACAAGCGTGGGGGTAATCC
TGTCGGGGCACAATACCGCT

Downstream 100 bases:

>100_bases
AAAAACAGCTTTTTTATTGCCCAAAAAACAGGCATCAGGGTATAATCCGACTTTCGACGGCAAGCGCGACAGCAACACCT
CCGGCACCGGCCCCCTTCAA

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 660; Mature: 659

Protein sequence:

>660_residues
MAIPQSFIQELLARADVVDIVGRHVQLKKGGANFMGLCPFHGEKSPSFSVSPSKQFYHCFGCGKNGNVISFLMEHAGMSF
IEAVKDLAQQYGLQVPEDDVSPQDRAKAAQMREQQATLTSVLEKAAMAYIKHLRGSERAIEYFKGRGLSGEIARTFGLGY
APEGWRSLASVFPDYNDPLLVESGLVITGEAGEENAGGEAKRYDRFRDRVMFPIRNVKGECIGFGGRVLGDEKPKYLNSP
ETPVFSKGRELYGLFEARTALREHGYALVTEGYMDVVALAQLGFANAVATLGTACTADHVQKLFRFTDSVVFSFDGDGAG
RRAARKALDAALPFATDVRTVKFLFLPSEHDPDSYIREHGKEGFAAYVSKAVPLSRFLLEAAAEGCDLDTAEGRAHMASN
ARPLWSLLPDGALKRQLLAEIADQVMIDSRELLQLWQPASAGYKNSYKNNSSKRLPNKDHRPDLPSDFGKNHDVPDYAGY
VPDLREEADYASSEPYFPPPAMSAQRPGRSSGGASRAPRRIAGRILPASREDRVLRLLLTEPQSWDQLSTEEHHLLLALP
APHGPLFVWLESQLHEHGPQPWAALREGLREHPHEKHAIAQIAQIPEGIECDWDEVRSILDQLVRLERQREMDELVPLVT
KDPAARQRYQELLEANLKKS

Sequences:

>Translated_660_residues
MAIPQSFIQELLARADVVDIVGRHVQLKKGGANFMGLCPFHGEKSPSFSVSPSKQFYHCFGCGKNGNVISFLMEHAGMSF
IEAVKDLAQQYGLQVPEDDVSPQDRAKAAQMREQQATLTSVLEKAAMAYIKHLRGSERAIEYFKGRGLSGEIARTFGLGY
APEGWRSLASVFPDYNDPLLVESGLVITGEAGEENAGGEAKRYDRFRDRVMFPIRNVKGECIGFGGRVLGDEKPKYLNSP
ETPVFSKGRELYGLFEARTALREHGYALVTEGYMDVVALAQLGFANAVATLGTACTADHVQKLFRFTDSVVFSFDGDGAG
RRAARKALDAALPFATDVRTVKFLFLPSEHDPDSYIREHGKEGFAAYVSKAVPLSRFLLEAAAEGCDLDTAEGRAHMASN
ARPLWSLLPDGALKRQLLAEIADQVMIDSRELLQLWQPASAGYKNSYKNNSSKRLPNKDHRPDLPSDFGKNHDVPDYAGY
VPDLREEADYASSEPYFPPPAMSAQRPGRSSGGASRAPRRIAGRILPASREDRVLRLLLTEPQSWDQLSTEEHHLLLALP
APHGPLFVWLESQLHEHGPQPWAALREGLREHPHEKHAIAQIAQIPEGIECDWDEVRSILDQLVRLERQREMDELVPLVT
KDPAARQRYQELLEANLKKS
>Mature_659_residues
AIPQSFIQELLARADVVDIVGRHVQLKKGGANFMGLCPFHGEKSPSFSVSPSKQFYHCFGCGKNGNVISFLMEHAGMSFI
EAVKDLAQQYGLQVPEDDVSPQDRAKAAQMREQQATLTSVLEKAAMAYIKHLRGSERAIEYFKGRGLSGEIARTFGLGYA
PEGWRSLASVFPDYNDPLLVESGLVITGEAGEENAGGEAKRYDRFRDRVMFPIRNVKGECIGFGGRVLGDEKPKYLNSPE
TPVFSKGRELYGLFEARTALREHGYALVTEGYMDVVALAQLGFANAVATLGTACTADHVQKLFRFTDSVVFSFDGDGAGR
RAARKALDAALPFATDVRTVKFLFLPSEHDPDSYIREHGKEGFAAYVSKAVPLSRFLLEAAAEGCDLDTAEGRAHMASNA
RPLWSLLPDGALKRQLLAEIADQVMIDSRELLQLWQPASAGYKNSYKNNSSKRLPNKDHRPDLPSDFGKNHDVPDYAGYV
PDLREEADYASSEPYFPPPAMSAQRPGRSSGGASRAPRRIAGRILPASREDRVLRLLLTEPQSWDQLSTEEHHLLLALPA
PHGPLFVWLESQLHEHGPQPWAALREGLREHPHEKHAIAQIAQIPEGIECDWDEVRSILDQLVRLERQREMDELVPLVTK
DPAARQRYQELLEANLKKS

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=444, Percent_Identity=45.945945945946, Blast_Score=394, Evalue=1e-110,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 73243; Mature: 73111

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIPQSFIQELLARADVVDIVGRHVQLKKGGANFMGLCPFHGEKSPSFSVSPSKQFYHCF
CCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEECCCCCCCCCCCCCCCCCCEEEEE
GCGKNGNVISFLMEHAGMSFIEAVKDLAQQYGLQVPEDDVSPQDRAKAAQMREQQATLTS
CCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
VLEKAAMAYIKHLRGSERAIEYFKGRGLSGEIARTFGLGYAPEGWRSLASVFPDYNDPLL
HHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHCCCCCCHHHHHHHHHCCCCCCCEE
VESGLVITGEAGEENAGGEAKRYDRFRDRVMFPIRNVKGECIGFGGRVLGDEKPKYLNSP
EECCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCEECCCCCCCCCCCC
ETPVFSKGRELYGLFEARTALREHGYALVTEGYMDVVALAQLGFANAVATLGTACTADHV
CCCHHHCCHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QKLFRFTDSVVFSFDGDGAGRRAARKALDAALPFATDVRTVKFLFLPSEHDPDSYIREHG
HHHHHHHHCEEEEECCCCCHHHHHHHHHHHHCCCHHCCCEEEEEEECCCCCCHHHHHHCC
KEGFAAYVSKAVPLSRFLLEAAAEGCDLDTAEGRAHMASNARPLWSLLPDGALKRQLLAE
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCHHHHHCCCHHHHHHHHHH
IADQVMIDSRELLQLWQPASAGYKNSYKNNSSKRLPNKDHRPDLPSDFGKNHDVPDYAGY
HHHHHHHCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCHHCC
VPDLREEADYASSEPYFPPPAMSAQRPGRSSGGASRAPRRIAGRILPASREDRVLRLLLT
CCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCEEHHHCC
EPQSWDQLSTEEHHLLLALPAPHGPLFVWLESQLHEHGPQPWAALREGLREHPHEKHAIA
CCCCCCCCCCCCCEEEEEECCCCCCEEEEEEHHHHHCCCCCHHHHHHHHHHCCCHHHHHH
QIAQIPEGIECDWDEVRSILDQLVRLERQREMDELVPLVTKDPAARQRYQELLEANLKKS
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AIPQSFIQELLARADVVDIVGRHVQLKKGGANFMGLCPFHGEKSPSFSVSPSKQFYHCF
CCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEECCCCCCCCCCCCCCCCCCEEEEE
GCGKNGNVISFLMEHAGMSFIEAVKDLAQQYGLQVPEDDVSPQDRAKAAQMREQQATLTS
CCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
VLEKAAMAYIKHLRGSERAIEYFKGRGLSGEIARTFGLGYAPEGWRSLASVFPDYNDPLL
HHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHCCCCCCHHHHHHHHHCCCCCCCEE
VESGLVITGEAGEENAGGEAKRYDRFRDRVMFPIRNVKGECIGFGGRVLGDEKPKYLNSP
EECCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCEECCCCCCCCCCCC
ETPVFSKGRELYGLFEARTALREHGYALVTEGYMDVVALAQLGFANAVATLGTACTADHV
CCCHHHCCHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QKLFRFTDSVVFSFDGDGAGRRAARKALDAALPFATDVRTVKFLFLPSEHDPDSYIREHG
HHHHHHHHCEEEEECCCCCHHHHHHHHHHHHCCCHHCCCEEEEEEECCCCCCHHHHHHCC
KEGFAAYVSKAVPLSRFLLEAAAEGCDLDTAEGRAHMASNARPLWSLLPDGALKRQLLAE
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCHHHHHCCCHHHHHHHHHH
IADQVMIDSRELLQLWQPASAGYKNSYKNNSSKRLPNKDHRPDLPSDFGKNHDVPDYAGY
HHHHHHHCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCHHCC
VPDLREEADYASSEPYFPPPAMSAQRPGRSSGGASRAPRRIAGRILPASREDRVLRLLLT
CCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCEEHHHCC
EPQSWDQLSTEEHHLLLALPAPHGPLFVWLESQLHEHGPQPWAALREGLREHPHEKHAIA
CCCCCCCCCCCCCEEEEEECCCCCCEEEEEEHHHHHCCCCCHHHHHHHHHHCCCHHHHHH
QIAQIPEGIECDWDEVRSILDQLVRLERQREMDELVPLVTKDPAARQRYQELLEANLKKS
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]