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The map label for this gene is dmlR [H]

Identifier: 91788928

GI number: 91788928

Start: 3234091

End: 3235107

Strand: Direct

Name: dmlR [H]

Synonym: Bpro_3068

Alternate gene names: 91788928

Gene position: 3234091-3235107 (Clockwise)

Preceding gene: 91788923

Following gene: 91788933

Centisome position: 62.19

GC content: 67.06

Gene sequence:

>1017_bases
ATGGATCGCAGCGATCTGGAGCTGGTACTGGCCGTCCGCGAGCACGGCAGTCTGACGGCGGCCGCCCTCAGCCTCGATGT
GGCGCCGCCCGTGGTGACCAAGCGGCTTGCCGCGCTGGAGGCCCGCCTGGGCCAGCGACTGTTCCAACGCACGACCCGGC
GCGTCAGCGCAACGGCCGAGGGCGAGACCATGTGCGAACGTGCCCTCGCACTGCTGCAAGGCTTTACCGCGCTGGAAGCC
GAGCTCCAGGAGCGCCAGTCGGAGCCCACGGGTTTGATCCGGCTGGCAGCGACGTTTGGCTTCGGGCGTTTGTGGCTGGG
CCCGGCGCTGGCCGACTTTCAGGAGCGCTATCCGCGCATCGAGATCCAGTTGCAACTGACCGAGCAATTGCCCGACCTTG
CGGAGGGCGGATTCGACGGCGCCATCTGGCTGTGGGAAGTTGAAGGCCGACAGGCGGCGCAATGGGTCTCACGCCGGCTT
GCGCGCAATCAGCGCGTGCTGGTGGCATCGCCCCGCTACATCCGGCAACACGGCGCACCGGCCAGCCTGGAAGACTTGCA
GCGCCACACCTGCCTGATCGTGCGCGAAAACGGCAATGCCACCGGCCAGCGCTTTGATGTCTGGCCGCTGCACAAGGAGC
GCGAAAAAACACCGGCGCGGGTACGGGTACGCGGGCCGCTGTCGAGCAACTCGGGTGAACTGGTACGTGACTGGTGTGTC
GAGGGCCGCGGCATCATGCTGCGCAGCCTCTGGGATATTGCACCCCAACTGGCATCCGGCGAGCTGGTGCGCGTGCTGCC
GGGCTACGCCATGCCCGATGCCGATATTCACTGGCTGGCCCCCTTCCGGCCCGATTCACCGCGGCGTATCCGCCTGCTCG
TTGACTTTTTGCTCGCCCAGTTTCAGGGTGCACCGTGGAAAAACAGTACGGTGACGGATGCCCGCAGGCGTTCCTTCGCT
ACGCCGCCGCCGGAGGCTTCGCGGACCTCACCACGAAGCTCACGCCCAGCGCGGTGA

Upstream 100 bases:

>100_bases
CAGATCTCGTGGGCCTCAGTGATTTTCAGTCGATTGTGCAGAAACAGGGATCCCGATTCAAGCAATAATCAGGCAATTGA
TTATTGCTTTAAAGGAATAA

Downstream 100 bases:

>100_bases
GCGCCGTACCCACCACGGTAGTCATGGTGATGGCCTCATCAAACAGCAACCAGGCCATCAGGGCCGTCGTCGGCGGCACC
AGGTAAAGCAGGCTGGTCAC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MDRSDLELVLAVREHGSLTAAALSLDVAPPVVTKRLAALEARLGQRLFQRTTRRVSATAEGETMCERALALLQGFTALEA
ELQERQSEPTGLIRLAATFGFGRLWLGPALADFQERYPRIEIQLQLTEQLPDLAEGGFDGAIWLWEVEGRQAAQWVSRRL
ARNQRVLVASPRYIRQHGAPASLEDLQRHTCLIVRENGNATGQRFDVWPLHKEREKTPARVRVRGPLSSNSGELVRDWCV
EGRGIMLRSLWDIAPQLASGELVRVLPGYAMPDADIHWLAPFRPDSPRRIRLLVDFLLAQFQGAPWKNSTVTDARRRSFA
TPPPEASRTSPRSSRPAR

Sequences:

>Translated_338_residues
MDRSDLELVLAVREHGSLTAAALSLDVAPPVVTKRLAALEARLGQRLFQRTTRRVSATAEGETMCERALALLQGFTALEA
ELQERQSEPTGLIRLAATFGFGRLWLGPALADFQERYPRIEIQLQLTEQLPDLAEGGFDGAIWLWEVEGRQAAQWVSRRL
ARNQRVLVASPRYIRQHGAPASLEDLQRHTCLIVRENGNATGQRFDVWPLHKEREKTPARVRVRGPLSSNSGELVRDWCV
EGRGIMLRSLWDIAPQLASGELVRVLPGYAMPDADIHWLAPFRPDSPRRIRLLVDFLLAQFQGAPWKNSTVTDARRRSFA
TPPPEASRTSPRSSRPAR
>Mature_338_residues
MDRSDLELVLAVREHGSLTAAALSLDVAPPVVTKRLAALEARLGQRLFQRTTRRVSATAEGETMCERALALLQGFTALEA
ELQERQSEPTGLIRLAATFGFGRLWLGPALADFQERYPRIEIQLQLTEQLPDLAEGGFDGAIWLWEVEGRQAAQWVSRRL
ARNQRVLVASPRYIRQHGAPASLEDLQRHTCLIVRENGNATGQRFDVWPLHKEREKTPARVRVRGPLSSNSGELVRDWCV
EGRGIMLRSLWDIAPQLASGELVRVLPGYAMPDADIHWLAPFRPDSPRRIRLLVDFLLAQFQGAPWKNSTVTDARRRSFA
TPPPEASRTSPRSSRPAR

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=298, Percent_Identity=38.9261744966443, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1789440, Length=300, Percent_Identity=37, Blast_Score=193, Evalue=1e-50,
Organism=Escherichia coli, GI1786401, Length=303, Percent_Identity=32.3432343234323, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI145693193, Length=300, Percent_Identity=29.6666666666667, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1787589, Length=302, Percent_Identity=27.4834437086093, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787128, Length=298, Percent_Identity=26.1744966442953, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1789639, Length=294, Percent_Identity=24.1496598639456, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1788706, Length=251, Percent_Identity=27.4900398406374, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI157672245, Length=124, Percent_Identity=37.0967741935484, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1788481, Length=261, Percent_Identity=26.8199233716475, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 37955; Mature: 37955

Theoretical pI: Translated: 10.53; Mature: 10.53

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRSDLELVLAVREHGSLTAAALSLDVAPPVVTKRLAALEARLGQRLFQRTTRRVSATAE
CCCCCCEEEEEEHHCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GETMCERALALLQGFTALEAELQERQSEPTGLIRLAATFGFGRLWLGPALADFQERYPRI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHCCCHHHHHHHHHHHHHHCCCE
EIQLQLTEQLPDLAEGGFDGAIWLWEVEGRQAAQWVSRRLARNQRVLVASPRYIRQHGAP
EEEEEEHHHCCHHHCCCCCCEEEEEEECCHHHHHHHHHHHHCCCEEEEECCHHHHHCCCC
ASLEDLQRHTCLIVRENGNATGQRFDVWPLHKEREKTPARVRVRGPLSSNSGELVRDWCV
CCHHHHCCCEEEEEEECCCCCCCEEEECCCCHHHCCCCCEEEEECCCCCCCCHHHHHHHH
EGRGIMLRSLWDIAPQLASGELVRVLPGYAMPDADIHWLAPFRPDSPRRIRLLVDFLLAQ
CCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHH
FQGAPWKNSTVTDARRRSFATPPPEASRTSPRSSRPAR
HCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MDRSDLELVLAVREHGSLTAAALSLDVAPPVVTKRLAALEARLGQRLFQRTTRRVSATAE
CCCCCCEEEEEEHHCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GETMCERALALLQGFTALEAELQERQSEPTGLIRLAATFGFGRLWLGPALADFQERYPRI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHCCCHHHHHHHHHHHHHHCCCE
EIQLQLTEQLPDLAEGGFDGAIWLWEVEGRQAAQWVSRRLARNQRVLVASPRYIRQHGAP
EEEEEEHHHCCHHHCCCCCCEEEEEEECCHHHHHHHHHHHHCCCEEEEECCHHHHHCCCC
ASLEDLQRHTCLIVRENGNATGQRFDVWPLHKEREKTPARVRVRGPLSSNSGELVRDWCV
CCHHHHCCCEEEEEEECCCCCCCEEEECCCCHHHCCCCCEEEEECCCCCCCCHHHHHHHH
EGRGIMLRSLWDIAPQLASGELVRVLPGYAMPDADIHWLAPFRPDSPRRIRLLVDFLLAQ
CCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHH
FQGAPWKNSTVTDARRRSFATPPPEASRTSPRSSRPAR
HCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]