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The map label for this gene is selU [H]

Identifier: 91788892

GI number: 91788892

Start: 3196385

End: 3197443

Strand: Direct

Name: selU [H]

Synonym: Bpro_3032

Alternate gene names: 91788892

Gene position: 3196385-3197443 (Clockwise)

Preceding gene: 91788891

Following gene: 91788898

Centisome position: 61.47

GC content: 64.87

Gene sequence:

>1059_bases
GTGAGCCTCGAACGCATTTCCGCCGAGGACGCTCTCGGCAAACTGGGCAGTTTTTCCGCCATCATTGACGCCCGCAGCGA
AGGTGAATACGCGGAAGACCACCTGCCTGGCGCCGTCAACTGGCCCAGCCTGAAGGACGACGAGCGAAAAATAGTCGGCA
CCCAGTACAAGCAGATCAACGCGTTTGAAGCCAAAAAGCAGGGTGCGGCGCTGGTGGCCAGGAACATTGCCGCCCACATC
GAGCGCGAAGTGCTGGACAAGCCGCGGGAATGGCAGCCGCTGGTGTATTGCTGGCGCGGCGGCAAACGCAGCGGCTCGCT
GGCGCTCATCCTCGACCAGATCGGCTTCAAGGTCACCCTGGTCGATGGCGGCTACAAGGCCTTCCGGGCGGCGCTGGTGG
CTGATTTGCCGCAGCTGGCAGCACGCCACAGCTACCAGGTTGTTTGCGGCACCACCGGTTCGGGCAAGACCCGCCTGCTC
CAGGCGCTGGCGGCCCAGGGTGCGCAGGTGCTGGACCTCGAAGCCCTGGCCAACCACCGCAGTTCGGTACTGGGCATGAT
TCCGGGCTCGCCGCAGCCCAGCCAGAAAGCCTTTGACAGCCGCATCTGGGCCGCCTTGCGCGGCTTTGACCCGACTCGCC
CGGTGTACATCGAGAGCGAAAGCAAAAAAGTCGGCAATGTGGCCGTGCCCGAAGGGCTGATTGCCGCCATGCGTGCCAGC
CCCTGCCTGCAGCTGGAACTGCCCGAAGACGAACGGGTGGCGCTGCTGCTGGAGGACTATGATTTTTTTGTCAAGGACAT
CGCCTTTTTCTGCGACCGGCTGGGCGCCCTGACCGAGGCGCGCGGCAAGGAAACCGTGCAGGACTGGCAGGCACGGGCCC
GCAACGGCGACGTGGCCTCGGTGGTTCGCGAATTGCTGGTCAAGCACTATGACCCGGTCTACCTGCAGTCGATGCGGCGC
AATTTCACCCACTACGCCACGGCGCGGGTACTGCAGCCGCAGGACCACAGCGTGGCTGCCATGGCGGCGCTGGCGCGGGA
AATGCTGGCCCGGCCCTGA

Upstream 100 bases:

>100_bases
ACCCCGTCGCCAACTTCCAGGCCGACCGCATTCACCCCAACGAACAGTCGCAAGCCAGGATGCTGGCCAATGTCTGGCCC
GAACTGAAGAAACTGATCAA

Downstream 100 bases:

>100_bases
AAGAGGCCTCTCGGCGCGGTCCGTCGTGCCGCGTCTAGAAATCCCCGCTCGATCCGCTGGCCAGACTCTCAAACTTGGTG
ATGCGGTTGATAAACGCCAG

Product: tRNA 2-selenouridine synthase

Products: NA

Alternate protein names: Selenophosphate-dependent tRNA 2-selenouridine synthase [H]

Number of amino acids: Translated: 352; Mature: 351

Protein sequence:

>352_residues
MSLERISAEDALGKLGSFSAIIDARSEGEYAEDHLPGAVNWPSLKDDERKIVGTQYKQINAFEAKKQGAALVARNIAAHI
EREVLDKPREWQPLVYCWRGGKRSGSLALILDQIGFKVTLVDGGYKAFRAALVADLPQLAARHSYQVVCGTTGSGKTRLL
QALAAQGAQVLDLEALANHRSSVLGMIPGSPQPSQKAFDSRIWAALRGFDPTRPVYIESESKKVGNVAVPEGLIAAMRAS
PCLQLELPEDERVALLLEDYDFFVKDIAFFCDRLGALTEARGKETVQDWQARARNGDVASVVRELLVKHYDPVYLQSMRR
NFTHYATARVLQPQDHSVAAMAALAREMLARP

Sequences:

>Translated_352_residues
MSLERISAEDALGKLGSFSAIIDARSEGEYAEDHLPGAVNWPSLKDDERKIVGTQYKQINAFEAKKQGAALVARNIAAHI
EREVLDKPREWQPLVYCWRGGKRSGSLALILDQIGFKVTLVDGGYKAFRAALVADLPQLAARHSYQVVCGTTGSGKTRLL
QALAAQGAQVLDLEALANHRSSVLGMIPGSPQPSQKAFDSRIWAALRGFDPTRPVYIESESKKVGNVAVPEGLIAAMRAS
PCLQLELPEDERVALLLEDYDFFVKDIAFFCDRLGALTEARGKETVQDWQARARNGDVASVVRELLVKHYDPVYLQSMRR
NFTHYATARVLQPQDHSVAAMAALAREMLARP
>Mature_351_residues
SLERISAEDALGKLGSFSAIIDARSEGEYAEDHLPGAVNWPSLKDDERKIVGTQYKQINAFEAKKQGAALVARNIAAHIE
REVLDKPREWQPLVYCWRGGKRSGSLALILDQIGFKVTLVDGGYKAFRAALVADLPQLAARHSYQVVCGTTGSGKTRLLQ
ALAAQGAQVLDLEALANHRSSVLGMIPGSPQPSQKAFDSRIWAALRGFDPTRPVYIESESKKVGNVAVPEGLIAAMRASP
CLQLELPEDERVALLLEDYDFFVKDIAFFCDRLGALTEARGKETVQDWQARARNGDVASVVRELLVKHYDPVYLQSMRRN
FTHYATARVLQPQDHSVAAMAALAREMLARP

Specific function: Catalyzes the transfer of selenium from selenophosphate for conversion of 2-thiouridine to 2-selenouridine at the wobble position in tRNA [H]

COG id: COG2603

COG function: function code R; Predicted ATPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

Organism=Escherichia coli, GI1786712, Length=320, Percent_Identity=31.5625, Blast_Score=110, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR017582 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: NA

Molecular weight: Translated: 38755; Mature: 38623

Theoretical pI: Translated: 7.98; Mature: 7.98

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLERISAEDALGKLGSFSAIIDARSEGEYAEDHLPGAVNWPSLKDDERKIVGTQYKQIN
CCCHHHCHHHHHHHHCCHHHHEECCCCCCCHHHCCCCCCCCCCCCCCCHHHHCCHHHHHH
AFEAKKQGAALVARNIAAHIEREVLDKPREWQPLVYCWRGGKRSGSLALILDQIGFKVTL
HHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCEEEEEECCCCEEEE
VDGGYKAFRAALVADLPQLAARHSYQVVCGTTGSGKTRLLQALAAQGAQVLDLEALANHR
ECCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCCEEEHHHHHCCH
SSVLGMIPGSPQPSQKAFDSRIWAALRGFDPTRPVYIESESKKVGNVAVPEGLIAAMRAS
HCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHCC
PCLQLELPEDERVALLLEDYDFFVKDIAFFCDRLGALTEARGKETVQDWQARARNGDVAS
CCEEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHH
VVRELLVKHYDPVYLQSMRRNFTHYATARVLQPQDHSVAAMAALAREMLARP
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SLERISAEDALGKLGSFSAIIDARSEGEYAEDHLPGAVNWPSLKDDERKIVGTQYKQIN
CCHHHCHHHHHHHHCCHHHHEECCCCCCCHHHCCCCCCCCCCCCCCCHHHHCCHHHHHH
AFEAKKQGAALVARNIAAHIEREVLDKPREWQPLVYCWRGGKRSGSLALILDQIGFKVTL
HHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCEEEEEECCCCEEEE
VDGGYKAFRAALVADLPQLAARHSYQVVCGTTGSGKTRLLQALAAQGAQVLDLEALANHR
ECCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCCEEEHHHHHCCH
SSVLGMIPGSPQPSQKAFDSRIWAALRGFDPTRPVYIESESKKVGNVAVPEGLIAAMRAS
HCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHCC
PCLQLELPEDERVALLLEDYDFFVKDIAFFCDRLGALTEARGKETVQDWQARARNGDVAS
CCEEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHH
VVRELLVKHYDPVYLQSMRRNFTHYATARVLQPQDHSVAAMAALAREMLARP
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]