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The map label for this gene is rhmD [H]

Identifier: 91788697

GI number: 91788697

Start: 2989677

End: 2990849

Strand: Direct

Name: rhmD [H]

Synonym: Bpro_2836

Alternate gene names: 91788697

Gene position: 2989677-2990849 (Clockwise)

Preceding gene: 91788696

Following gene: 91788698

Centisome position: 57.49

GC content: 59.34

Gene sequence:

>1173_bases
ATGAAAATCAAATCAGTTCGCGCGCGTGTGTTTGAGTGGAAAGGCAAAACCGTTCCGCCGCAGGGTAATTTCTGCTCCAA
CGCCATGGACCTGCTCTACTCCAACAAGGAGACCATGAGCACCTTCCGCTTCCACTCCTGGACGGTGGTTGAAATCGAAA
CCGACGACGGCATCATCGGCCTGGGCAACGTGGCGCTGGCGCCCGCCATCGCCAAAGCCATCATTGACCAGTACCTCACG
CCGCTGGTGCTGGGCCAGGACCCGTGGGATTACGAATACCTGTGGCAGCGCATGTACCGCGCCACGCACGCCTGGGGCCG
CAAGGGCGTGACCATGGCCGCCATCTCGGCGATTGACCTGGCTATCTGGGACATCCTGGGCAAATCGGTGAACAAGCCGG
TGTTCAAGCTGCTGGGCGGCCGCACCAAGGAAAAAATCCCCTGCTACTACTCCAAGCTCTACCGCACCGACATCAAGGCC
ATGCAGGACGAAGCGCAGAAATACCTGGACCAGGGTTTCACCATGTTCAAGTCGCGCTTCGGCTATGGCCCGGCGCACGG
CACCAGGGGCGTGGCGGAAAACCTGAAGGCGGTGGAAGCGATTCGCGAAGTCATTGGCTACGACAACGACCTGATGCTCG
AGTGCTACATGGGCTGGAACCTGGAGTACGCCAAGCGCATGCTGCCCAAGCTGGAGAAATTCCAGCCGCGCTGGGTGGAA
GAACCGGTGATCGCCGACGACATTGACGGCTATGCCGAACTCAACCAGCTCACCAGCATCCCGATCTCTGGCGGCGAGCA
TGAGTTTTCGCTCTACGGCTTCAAGCAGCTGCTCGACAAAAAAGCGGTGTCGGTGGTGCAGTACGACACCAACCGCGTGG
GCGGCATCACGGCGGCCCACAAGATCAATGCCTTGTGCGAGGCCTACAGCGTGCCGGTGATTCCGCATGCCGGGCAGATG
CACAACTACCACCTGACCATGAGCACGCTGGCCTCGCCCATGAGCGAGTACTTCCCGATGTTCGACGTGGAAGTAGGCAA
CGAGCTCTTCTATTACATCTTCGACGGCGAACCGGTGGCAGAAAACGGCTTCCTGCAGCTTGACGACAACAAGCCCGGCC
TGGGCCTGACGCTCAAGACCGAGTTCCTCGAAGACTTCAACATCATTGAGTGA

Upstream 100 bases:

>100_bases
AGCGCATGGGTACCGCCCGGCGGGAGCAGACTGCTTGAGTGTTCACAGTCTCGCTACAACAAGCGACACATTCATTTTTT
TAATCCGTTAAAGAATCATC

Downstream 100 bases:

>100_bases
GCACCATGGCAGCCAGGTTCAAGGGCGTATTTCCGGTGGTGCCCACCACCTTCAACGATGCGGGCGAGCTGGACCTCGAA
AGCCAGAAGCGCTGCATCGA

Product: mandelate racemase/muconate lactonizing protein

Products: NA

Alternate protein names: RhamD [H]

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MKIKSVRARVFEWKGKTVPPQGNFCSNAMDLLYSNKETMSTFRFHSWTVVEIETDDGIIGLGNVALAPAIAKAIIDQYLT
PLVLGQDPWDYEYLWQRMYRATHAWGRKGVTMAAISAIDLAIWDILGKSVNKPVFKLLGGRTKEKIPCYYSKLYRTDIKA
MQDEAQKYLDQGFTMFKSRFGYGPAHGTRGVAENLKAVEAIREVIGYDNDLMLECYMGWNLEYAKRMLPKLEKFQPRWVE
EPVIADDIDGYAELNQLTSIPISGGEHEFSLYGFKQLLDKKAVSVVQYDTNRVGGITAAHKINALCEAYSVPVIPHAGQM
HNYHLTMSTLASPMSEYFPMFDVEVGNELFYYIFDGEPVAENGFLQLDDNKPGLGLTLKTEFLEDFNIIE

Sequences:

>Translated_390_residues
MKIKSVRARVFEWKGKTVPPQGNFCSNAMDLLYSNKETMSTFRFHSWTVVEIETDDGIIGLGNVALAPAIAKAIIDQYLT
PLVLGQDPWDYEYLWQRMYRATHAWGRKGVTMAAISAIDLAIWDILGKSVNKPVFKLLGGRTKEKIPCYYSKLYRTDIKA
MQDEAQKYLDQGFTMFKSRFGYGPAHGTRGVAENLKAVEAIREVIGYDNDLMLECYMGWNLEYAKRMLPKLEKFQPRWVE
EPVIADDIDGYAELNQLTSIPISGGEHEFSLYGFKQLLDKKAVSVVQYDTNRVGGITAAHKINALCEAYSVPVIPHAGQM
HNYHLTMSTLASPMSEYFPMFDVEVGNELFYYIFDGEPVAENGFLQLDDNKPGLGLTLKTEFLEDFNIIE
>Mature_390_residues
MKIKSVRARVFEWKGKTVPPQGNFCSNAMDLLYSNKETMSTFRFHSWTVVEIETDDGIIGLGNVALAPAIAKAIIDQYLT
PLVLGQDPWDYEYLWQRMYRATHAWGRKGVTMAAISAIDLAIWDILGKSVNKPVFKLLGGRTKEKIPCYYSKLYRTDIKA
MQDEAQKYLDQGFTMFKSRFGYGPAHGTRGVAENLKAVEAIREVIGYDNDLMLECYMGWNLEYAKRMLPKLEKFQPRWVE
EPVIADDIDGYAELNQLTSIPISGGEHEFSLYGFKQLLDKKAVSVVQYDTNRVGGITAAHKINALCEAYSVPVIPHAGQM
HNYHLTMSTLASPMSEYFPMFDVEVGNELFYYIFDGEPVAENGFLQLDDNKPGLGLTLKTEFLEDFNIIE

Specific function: Catalyzes the dehydration of L-rhamnonate to 2-keto-3- deoxy-L-rhamnonate (KDR) [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. RhamD subfamily [H]

Homologues:

Organism=Homo sapiens, GI42544119, Length=368, Percent_Identity=24.7282608695652, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI186972148, Length=271, Percent_Identity=26.9372693726937, Blast_Score=99, Evalue=9e-21,
Organism=Homo sapiens, GI42544117, Length=326, Percent_Identity=25.4601226993865, Blast_Score=96, Evalue=5e-20,
Organism=Escherichia coli, GI48994953, Length=360, Percent_Identity=30.5555555555556, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI226510960, Length=338, Percent_Identity=28.9940828402367, Blast_Score=145, Evalue=3e-36,
Organism=Escherichia coli, GI1787864, Length=376, Percent_Identity=26.8617021276596, Blast_Score=125, Evalue=6e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.90 [H]

Molecular weight: Translated: 44236; Mature: 44236

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKSVRARVFEWKGKTVPPQGNFCSNAMDLLYSNKETMSTFRFHSWTVVEIETDDGIIG
CCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCHHHHHHEEECCEEEEEEECCCCEEE
LGNVALAPAIAKAIIDQYLTPLVLGQDPWDYEYLWQRMYRATHAWGRKGVTMAAISAIDL
ECHHHHHHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
AIWDILGKSVNKPVFKLLGGRTKEKIPCYYSKLYRTDIKAMQDEAQKYLDQGFTMFKSRF
HHHHHHHHHCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GYGPAHGTRGVAENLKAVEAIREVIGYDNDLMLECYMGWNLEYAKRMLPKLEKFQPRWVE
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCCC
EPVIADDIDGYAELNQLTSIPISGGEHEFSLYGFKQLLDKKAVSVVQYDTNRVGGITAAH
CCCCCCCCCCHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHEEECCCCCCCCHHHH
KINALCEAYSVPVIPHAGQMHNYHLTMSTLASPMSEYFPMFDVEVGNELFYYIFDGEPVA
HHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHHHHHHHCCEEEEEECCEEEEEEECCCEEC
ENGFLQLDDNKPGLGLTLKTEFLEDFNIIE
CCCCEEECCCCCCCEEEEEHHHHCCCCCCC
>Mature Secondary Structure
MKIKSVRARVFEWKGKTVPPQGNFCSNAMDLLYSNKETMSTFRFHSWTVVEIETDDGIIG
CCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCHHHHHHEEECCEEEEEEECCCCEEE
LGNVALAPAIAKAIIDQYLTPLVLGQDPWDYEYLWQRMYRATHAWGRKGVTMAAISAIDL
ECHHHHHHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
AIWDILGKSVNKPVFKLLGGRTKEKIPCYYSKLYRTDIKAMQDEAQKYLDQGFTMFKSRF
HHHHHHHHHCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GYGPAHGTRGVAENLKAVEAIREVIGYDNDLMLECYMGWNLEYAKRMLPKLEKFQPRWVE
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCCC
EPVIADDIDGYAELNQLTSIPISGGEHEFSLYGFKQLLDKKAVSVVQYDTNRVGGITAAH
CCCCCCCCCCHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHEEECCCCCCCCHHHH
KINALCEAYSVPVIPHAGQMHNYHLTMSTLASPMSEYFPMFDVEVGNELFYYIFDGEPVA
HHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHHHHHHHCCEEEEEECCEEEEEEECCCEEC
ENGFLQLDDNKPGLGLTLKTEFLEDFNIIE
CCCCEEECCCCCCCEEEEEHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA