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The map label for this gene is ispDF [H]

Identifier: 91788577

GI number: 91788577

Start: 2860797

End: 2861306

Strand: Reverse

Name: ispDF [H]

Synonym: Bpro_2715

Alternate gene names: 91788577

Gene position: 2861306-2860797 (Counterclockwise)

Preceding gene: 91788578

Following gene: 91788574

Centisome position: 55.02

GC content: 64.71

Gene sequence:

>510_bases
ATGAATTCCGTGCCGTCCACCCCAGCCAACCCGCTTCCCCATTTCCGTATCGGCGAAGGCTGGGACACGCACGCACTCGT
TGCCGGGCGCAAGCTCATCATCGGCGGCGTCGAGATTCCGCACCCGGTGGGCTTGCTGGGGCATTCGGATGCCGATGTAC
TGCTGCATGCCATTACCGACGCCTTGCTGGGCGCTGCCGGGCTGGGTGACATTGGCAGCCATTTCCCGGACACCGATGTG
CGTTTCAAAGGCGCCGATTCGATGGTGCTGCTGGCTGAGGCCGGGCGGCTGCTGGCCGATCGGGGCTACAGTATCGGCAA
CATAGACAGCACCGTCATTGCGCAGGCCCCCAAACTGGCGCCGCACATCCTCGCTATGCGCGCGCGGATCGCACAGGCAC
TGGGCCTGGAACCGGGGCAGGTGAATGTCAAGGCCAAGACCGCCGAGAAGATGGGACCGGTCGGGGAGGGCCGGAGCATG
GAGGCTCGTGCCGTGGTGTTGCTGTATTGA

Upstream 100 bases:

>100_bases
CTCACGCGCAGCCGCAGACCCTGAAGCCGTTGCCGGGCCATCGTTGACCGCCCGGGAAACGATTCCCTTTTACTTTTATT
TTCATGTGAAAGCGCATGCC

Downstream 100 bases:

>100_bases
GGCTATACGTTTGATCGCGCAGGTTCGGGTGAGTTGGGTGAGTTGGGCACCCGGCACTGGCCTCAGCTTCTGCGCAGCTT
GATATGCGCGGCCAGCCCGC

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase; 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT; 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase; MECDP-synthase; MECPS [H]

Number of amino acids: Translated: 169; Mature: 169

Protein sequence:

>169_residues
MNSVPSTPANPLPHFRIGEGWDTHALVAGRKLIIGGVEIPHPVGLLGHSDADVLLHAITDALLGAAGLGDIGSHFPDTDV
RFKGADSMVLLAEAGRLLADRGYSIGNIDSTVIAQAPKLAPHILAMRARIAQALGLEPGQVNVKAKTAEKMGPVGEGRSM
EARAVVLLY

Sequences:

>Translated_169_residues
MNSVPSTPANPLPHFRIGEGWDTHALVAGRKLIIGGVEIPHPVGLLGHSDADVLLHAITDALLGAAGLGDIGSHFPDTDV
RFKGADSMVLLAEAGRLLADRGYSIGNIDSTVIAQAPKLAPHILAMRARIAQALGLEPGQVNVKAKTAEKMGPVGEGRSM
EARAVVLLY
>Mature_169_residues
MNSVPSTPANPLPHFRIGEGWDTHALVAGRKLIIGGVEIPHPVGLLGHSDADVLLHAITDALLGAAGLGDIGSHFPDTDV
RFKGADSMVLLAEAGRLLADRGYSIGNIDSTVIAQAPKLAPHILAMRARIAQALGLEPGQVNVKAKTAEKMGPVGEGRSM
EARAVVLLY

Specific function: Bifunctional enzyme that catalyzes the formation of 4- diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl- D-erythritol 4-phosphate (MEP) (ispD), and converts 4- diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C- methyl-D-erythri

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the ispF family [H]

Homologues:

Organism=Escherichia coli, GI1789103, Length=153, Percent_Identity=58.8235294117647, Blast_Score=161, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001228
- InterPro:   IPR018294
- InterPro:   IPR003526
- InterPro:   IPR020555 [H]

Pfam domain/function: PF01128 IspD; PF02542 YgbB [H]

EC number: =2.7.7.60; =4.6.1.12 [H]

Molecular weight: Translated: 17603; Mature: 17603

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSVPSTPANPLPHFRIGEGWDTHALVAGRKLIIGGVEIPHPVGLLGHSDADVLLHAITD
CCCCCCCCCCCCCCCEECCCCCCCEEECCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHH
ALLGAAGLGDIGSHFPDTDVRFKGADSMVLLAEAGRLLADRGYSIGNIDSTVIAQAPKLA
HHHCCCCCCCHHCCCCCCCEEEECCCCEEEEECCCHHHHHCCCCCCCCCCHHHHCCCCHH
PHILAMRARIAQALGLEPGQVNVKAKTAEKMGPVGEGRSMEARAVVLLY
HHHHHHHHHHHHHHCCCCCEEEEEECCHHHCCCCCCCCCCCCEEEEEEC
>Mature Secondary Structure
MNSVPSTPANPLPHFRIGEGWDTHALVAGRKLIIGGVEIPHPVGLLGHSDADVLLHAITD
CCCCCCCCCCCCCCCEECCCCCCCEEECCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHH
ALLGAAGLGDIGSHFPDTDVRFKGADSMVLLAEAGRLLADRGYSIGNIDSTVIAQAPKLA
HHHCCCCCCCHHCCCCCCCEEEECCCCEEEEECCCHHHHHCCCCCCCCCCHHHHCCCCHH
PHILAMRARIAQALGLEPGQVNVKAKTAEKMGPVGEGRSMEARAVVLLY
HHHHHHHHHHHHHHCCCCCEEEEEECCHHHCCCCCCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA