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The map label for this gene is sucB [H]

Identifier: 91788485

GI number: 91788485

Start: 2754370

End: 2755638

Strand: Reverse

Name: sucB [H]

Synonym: Bpro_2623

Alternate gene names: 91788485

Gene position: 2755638-2754370 (Counterclockwise)

Preceding gene: 91788486

Following gene: 91788484

Centisome position: 52.99

GC content: 61.62

Gene sequence:

>1269_bases
ATGGCTATCGTAGAAGTCAAAGTCCCCCAATTGTCCGAGTCCGTGGCGGAAGCCACCATGCTGCAGTGGAAAAAGAAAAT
CGGCGAGGCAATTGCCATCGACGAAATCCTGATTGAAATCGAAACCGACAAGGTCGTGCTGGAAGTGCCCGCGCCTTCGG
CCGGTGTGCTGACCGAGCTGGTGGTGGCCGATGGCGGCACCGTGGTGTCCGACCAGGTGATTGCGAGAATTGATACCGAG
GGCAAGGCAGGCGCTGGTGCGCCGGCTCCAGCGGCGGCAGCGGCCGCTGCTCCTGCAGCTGCAGCTTCATCTGTGCCGGC
CCCTGCGGCGGGTGGTTCCATGGCCGGTGTGCCCATGCCGGCTGCTGCCAAGCTGATGGCGGACGCCAACCTGGCGGCCG
GCTCTGTGCCTGGGACCGGCAAGGACGGCCGTGTCACCAAGGGTGATGTGCTCGGCGCAACAGCAGCCGGTGCTGTCAAA
TCTGTAGCTGCCAGCCCAGTTCCTACGGGGGCTCCGACCACTTCCTTGCCTCAGGTCGCCGCGCAGGTGAAGCCCGCTGA
CCTGGGCGATCGTCCCGAGCAGCGTGTGCCCATGAGCCGCCTGCGTGCGCGCGTGGCCGAGCGCCTGTTGCAGTCCCAAT
CCACCAACGCCATCCTCACTACCTTCAATGAAGTGAACATGGCTCCCGTGATGGAAATGCGCAAGCGATTCCAGGAGAAA
TTCGAGAAGGAGCATGGCGTCAAGATCGGCTTCATGAGCTTTTTTGTGAAGGCCGCAGTGCATGCGTTGAAGAAGTACCC
GGTCCTGAACGCGTCGGTCGACGGTAACGACATCGTCTACCACGGCTACTTCGACATCGGTATTGCTGTCGGTTCACCGC
GCGGCCTGGTGGTGCCCATCCTGCGCAATGCCGACCAGATGAGCTTTGCCGACATCGAGAAGAAGATCGCCGAATATGGT
GCCAAGGCGCGCGATGGCAAGCTGGGCATCGAAGAGATGACGGGTGGTACCTTCTCGATCTCCAACGGCGGTGTGTTCGG
CTCCATGCTGTCCACGCCCATCATCAACCCACCGCAGTCCGCCATTCTGGGCGTGCACGCCACGAAAGACCGGGCAGTCG
TCGAGAACGGCCAGATTGTGGTTCGCCCGATGAACTACCTGGCCATGAGCTATGACCACCGCATCATTGATGGCCGCGAA
GCCGTGCTGGGCCTGGTGGCCATGAAGGAAGCGCTGGAAGATCCGGCCCGCTTGCTGTTTGATATTTGA

Upstream 100 bases:

>100_bases
GTTTGGCAAGCTCAAGGGCTTTGTGCTGACCAAGTAAGAACCAGGATCCCCGGGCCTTCGTGCGCCCGCGGAACACAAGA
ACATATCGGAAAGAAACAAA

Downstream 100 bases:

>100_bases
CGCCCCTGTTGCCGCTCACTGCGTGTAGCGGCCTCCCCCCTCAAGGGGGCGACACCTGCGGCCCGGCAAAGCCGGTTCCG
CGGTGTCTCGCGAACAGGCT

Product: dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 422; Mature: 421

Protein sequence:

>422_residues
MAIVEVKVPQLSESVAEATMLQWKKKIGEAIAIDEILIEIETDKVVLEVPAPSAGVLTELVVADGGTVVSDQVIARIDTE
GKAGAGAPAPAAAAAAAPAAAASSVPAPAAGGSMAGVPMPAAAKLMADANLAAGSVPGTGKDGRVTKGDVLGATAAGAVK
SVAASPVPTGAPTTSLPQVAAQVKPADLGDRPEQRVPMSRLRARVAERLLQSQSTNAILTTFNEVNMAPVMEMRKRFQEK
FEKEHGVKIGFMSFFVKAAVHALKKYPVLNASVDGNDIVYHGYFDIGIAVGSPRGLVVPILRNADQMSFADIEKKIAEYG
AKARDGKLGIEEMTGGTFSISNGGVFGSMLSTPIINPPQSAILGVHATKDRAVVENGQIVVRPMNYLAMSYDHRIIDGRE
AVLGLVAMKEALEDPARLLFDI

Sequences:

>Translated_422_residues
MAIVEVKVPQLSESVAEATMLQWKKKIGEAIAIDEILIEIETDKVVLEVPAPSAGVLTELVVADGGTVVSDQVIARIDTE
GKAGAGAPAPAAAAAAAPAAAASSVPAPAAGGSMAGVPMPAAAKLMADANLAAGSVPGTGKDGRVTKGDVLGATAAGAVK
SVAASPVPTGAPTTSLPQVAAQVKPADLGDRPEQRVPMSRLRARVAERLLQSQSTNAILTTFNEVNMAPVMEMRKRFQEK
FEKEHGVKIGFMSFFVKAAVHALKKYPVLNASVDGNDIVYHGYFDIGIAVGSPRGLVVPILRNADQMSFADIEKKIAEYG
AKARDGKLGIEEMTGGTFSISNGGVFGSMLSTPIINPPQSAILGVHATKDRAVVENGQIVVRPMNYLAMSYDHRIIDGRE
AVLGLVAMKEALEDPARLLFDI
>Mature_421_residues
AIVEVKVPQLSESVAEATMLQWKKKIGEAIAIDEILIEIETDKVVLEVPAPSAGVLTELVVADGGTVVSDQVIARIDTEG
KAGAGAPAPAAAAAAAPAAAASSVPAPAAGGSMAGVPMPAAAKLMADANLAAGSVPGTGKDGRVTKGDVLGATAAGAVKS
VAASPVPTGAPTTSLPQVAAQVKPADLGDRPEQRVPMSRLRARVAERLLQSQSTNAILTTFNEVNMAPVMEMRKRFQEKF
EKEHGVKIGFMSFFVKAAVHALKKYPVLNASVDGNDIVYHGYFDIGIAVGSPRGLVVPILRNADQMSFADIEKKIAEYGA
KARDGKLGIEEMTGGTFSISNGGVFGSMLSTPIINPPQSAILGVHATKDRAVVENGQIVVRPMNYLAMSYDHRIIDGREA
VLGLVAMKEALEDPARLLFDI

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=452, Percent_Identity=30.0884955752212, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI203098753, Length=453, Percent_Identity=29.1390728476821, Blast_Score=162, Evalue=6e-40,
Organism=Homo sapiens, GI203098816, Length=453, Percent_Identity=29.1390728476821, Blast_Score=161, Evalue=9e-40,
Organism=Homo sapiens, GI110671329, Length=438, Percent_Identity=26.027397260274, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=37.2670807453416, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI19923748, Length=66, Percent_Identity=46.969696969697, Blast_Score=67, Evalue=5e-11,
Organism=Escherichia coli, GI1786946, Length=422, Percent_Identity=50.9478672985782, Blast_Score=433, Evalue=1e-122,
Organism=Escherichia coli, GI1786305, Length=430, Percent_Identity=32.5581395348837, Blast_Score=157, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI25146366, Length=418, Percent_Identity=44.7368421052632, Blast_Score=335, Evalue=3e-92,
Organism=Caenorhabditis elegans, GI17560088, Length=451, Percent_Identity=31.2638580931264, Blast_Score=170, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17537937, Length=432, Percent_Identity=27.5462962962963, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17538894, Length=305, Percent_Identity=31.1475409836066, Blast_Score=135, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6320352, Length=422, Percent_Identity=41.2322274881517, Blast_Score=314, Evalue=2e-86,
Organism=Saccharomyces cerevisiae, GI6324258, Length=448, Percent_Identity=27.0089285714286, Blast_Score=134, Evalue=4e-32,
Organism=Drosophila melanogaster, GI24645909, Length=243, Percent_Identity=55.5555555555556, Blast_Score=281, Evalue=5e-76,
Organism=Drosophila melanogaster, GI18859875, Length=447, Percent_Identity=28.6353467561521, Blast_Score=139, Evalue=5e-33,
Organism=Drosophila melanogaster, GI24582497, Length=230, Percent_Identity=31.7391304347826, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI20129315, Length=228, Percent_Identity=32.0175438596491, Blast_Score=125, Evalue=4e-29,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 43847; Mature: 43716

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIVEVKVPQLSESVAEATMLQWKKKIGEAIAIDEILIEIETDKVVLEVPAPSAGVLTEL
CEEEEEECCHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEECCEEEEECCCCCHHHHHHH
VVADGGTVVSDQVIARIDTEGKAGAGAPAPAAAAAAAPAAAASSVPAPAAGGSMAGVPMP
HHCCCCEEECCCEEEEECCCCCCCCCCCCCHHHHHHCCHHHHCCCCCCCCCCCCCCCCCC
AAAKLMADANLAAGSVPGTGKDGRVTKGDVLGATAAGAVKSVAASPVPTGAPTTSLPQVA
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHH
AQVKPADLGDRPEQRVPMSRLRARVAERLLQSQSTNAILTTFNEVNMAPVMEMRKRFQEK
HHCCCCCCCCCCHHCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHH
FEKEHGVKIGFMSFFVKAAVHALKKYPVLNASVDGNDIVYHGYFDIGIAVGSPRGLVVPI
HHHHHCCEEHHHHHHHHHHHHHHHHCCCEECCCCCCEEEEEEEEEEEEEECCCCCEEEEE
LRNADQMSFADIEKKIAEYGAKARDGKLGIEEMTGGTFSISNGGVFGSMLSTPIINPPQS
ECCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHCCCEEEECCCCEEHHHHCCCCCCCCHH
AILGVHATKDRAVVENGQIVVRPMNYLAMSYDHRIIDGREAVLGLVAMKEALEDPARLLF
HEEEEECCCCCEEEECCCEEEEEHHHHHHHCCCEEECCHHHHHHHHHHHHHHCCHHHHHH
DI
CC
>Mature Secondary Structure 
AIVEVKVPQLSESVAEATMLQWKKKIGEAIAIDEILIEIETDKVVLEVPAPSAGVLTEL
EEEEEECCHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEECCEEEEECCCCCHHHHHHH
VVADGGTVVSDQVIARIDTEGKAGAGAPAPAAAAAAAPAAAASSVPAPAAGGSMAGVPMP
HHCCCCEEECCCEEEEECCCCCCCCCCCCCHHHHHHCCHHHHCCCCCCCCCCCCCCCCCC
AAAKLMADANLAAGSVPGTGKDGRVTKGDVLGATAAGAVKSVAASPVPTGAPTTSLPQVA
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHH
AQVKPADLGDRPEQRVPMSRLRARVAERLLQSQSTNAILTTFNEVNMAPVMEMRKRFQEK
HHCCCCCCCCCCHHCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHH
FEKEHGVKIGFMSFFVKAAVHALKKYPVLNASVDGNDIVYHGYFDIGIAVGSPRGLVVPI
HHHHHCCEEHHHHHHHHHHHHHHHHCCCEECCCCCCEEEEEEEEEEEEEECCCCCEEEEE
LRNADQMSFADIEKKIAEYGAKARDGKLGIEEMTGGTFSISNGGVFGSMLSTPIINPPQS
ECCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHCCCEEEECCCCEEHHHHCCCCCCCCHH
AILGVHATKDRAVVENGQIVVRPMNYLAMSYDHRIIDGREAVLGLVAMKEALEDPARLLF
HEEEEECCCCCEEEECCCEEEEEHHHHHHHCCCEEECCHHHHHHHHHHHHHHCCHHHHHH
DI
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8867378 [H]