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The map label for this gene is odhL [H]

Identifier: 91788484

GI number: 91788484

Start: 2752775

End: 2754202

Strand: Reverse

Name: odhL [H]

Synonym: Bpro_2622

Alternate gene names: 91788484

Gene position: 2754202-2752775 (Counterclockwise)

Preceding gene: 91788485

Following gene: 91788483

Centisome position: 52.96

GC content: 61.48

Gene sequence:

>1428_bases
ATGTCCAAACAATTCGACGTGATCGTCATCGGCGGCGGCCCCGGCGGCTACATTGCCGCCATTCGCGCCGCCCAGTTGGG
TTTTAACGTCGCCTGTATCGACGAGTGGAAAAATGCCAAGGGCGGGCCTGCCCTGGGCGGCACCTGTACCAATGTAGGCT
GCATCCCGTCCAAGGCCCTGTTGCAGTCGTCCGAGCATTACGAGCAGGCCGGTCACCACTTCGCCGACCACGGCATTGAA
GTGAAGGGCCTGAGCCTGGACCTGGCCAAGATGGTGGGTCGAAAGGACACGGTCGTCAAGCAAAACAACGATGGCATCGT
GTACCTGTTCAAGAAGAACAAGGTCACCTTCTTCCATGGCCGCGCCTCCTTTGCCGCTGCCAAGGACGGTCTCTACGACA
TCAAGGTCGCGGGCGCTGCGGAAGAAACGCTTTCGGGCAAGCACATCATTGTGGCCACGGGTTCGAATGCCCGCGCGCTG
CCCGGCGCTCCTTTTGACGAAGAGAGCATTCTGTCGAACGACGGCGCGCTGCGCATCGGTGCCGTGCCCAAAAAGCTCGG
TTTGATCGGTTCCGGCGTGATCGGCCTCGAAATGGGTTCAGTCTGGCGCCGTCTGGGTGCCGAGGTCACGGTTCTGGAAG
GCCTGCCCACGTTCCTGGGTGCCGTTGACCAGCAGATTGCCAAAGAGGCGTACAAGGCCTTCGTCAAGCAGGGCCTGAAG
ATCGAGCTGGGTGTCCAGGTGGGCGAGGTCAAGTCGGGTAAAAACGGTGTCTCCGTGGCCTATGTGGATGCGAAGGGCCA
GGCCCAGAAAATTGATGTGGACAAGCTGATCGTCTCGATTGGCCGGGTTGCCAACACCATCGGACTGGCGCCTGAAACGG
TCGGGCTGAAGCTCGATGAGCGCGGCGCCATCGTGGTGGACGATGAATGCAAGACGAATTTGCCGAATGTGTGGGCCATC
GGCGACGTGGTGCGCGGGCCGATGCTGGCGCACAAGGCCGAGGAAGAAGGCGTTGCGGTCGCTGAACGCATTGCAGGCCA
GCACGGCCACGTCAACTTCAACACCATTCCGTGGGTGATTTACACCAACCCCGAGATCGCCTGGGTGGGCCAGACGGAAG
AGCAGCTGAAGGCCGCAGGACGCGCTTACCGGGCTGGCACCTTCCCCTTCATGGCCAATGGCCGGGCGCGCGCTTTGGGC
GACACCACCGGCATGGTCAAGATGCTGGCCGATACGGCCACGGATGAAATCCTGGGCGTGCACATTGTGGGACCGTTTGC
CAGCGAGTTGATTGCCGAATGTGTGGTGGCCATGGAGTTCCGCGCCAGCAGCGAGGACATCGCGCGCATCTGCCATGCCC
ATCCATCCTTGAGCGAGGCCACGAAAGAGGCCGCGCTGGCAGTGGACAAGCGTACCCTGAACTTCTGA

Upstream 100 bases:

>100_bases
AAAGCCGGTTCCGCGGTGTCTCGCGAACAGGCTTTGCTTCGCTCGCCGGTGCGAGAGCAAATCGAGGTATTGCCACCGGC
AGCTTTATTGAAAGAACAAC

Downstream 100 bases:

>100_bases
CACCCTGGATACCGGACCGGTGACACAGCTGGTGCGCTCGGCCTACGAGGCTGAACTGGTGGCGCGGGGTTACACGAGTG
ACCCCGCCCAGTTGCGTGCG

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MSKQFDVIVIGGGPGGYIAAIRAAQLGFNVACIDEWKNAKGGPALGGTCTNVGCIPSKALLQSSEHYEQAGHHFADHGIE
VKGLSLDLAKMVGRKDTVVKQNNDGIVYLFKKNKVTFFHGRASFAAAKDGLYDIKVAGAAEETLSGKHIIVATGSNARAL
PGAPFDEESILSNDGALRIGAVPKKLGLIGSGVIGLEMGSVWRRLGAEVTVLEGLPTFLGAVDQQIAKEAYKAFVKQGLK
IELGVQVGEVKSGKNGVSVAYVDAKGQAQKIDVDKLIVSIGRVANTIGLAPETVGLKLDERGAIVVDDECKTNLPNVWAI
GDVVRGPMLAHKAEEEGVAVAERIAGQHGHVNFNTIPWVIYTNPEIAWVGQTEEQLKAAGRAYRAGTFPFMANGRARALG
DTTGMVKMLADTATDEILGVHIVGPFASELIAECVVAMEFRASSEDIARICHAHPSLSEATKEAALAVDKRTLNF

Sequences:

>Translated_475_residues
MSKQFDVIVIGGGPGGYIAAIRAAQLGFNVACIDEWKNAKGGPALGGTCTNVGCIPSKALLQSSEHYEQAGHHFADHGIE
VKGLSLDLAKMVGRKDTVVKQNNDGIVYLFKKNKVTFFHGRASFAAAKDGLYDIKVAGAAEETLSGKHIIVATGSNARAL
PGAPFDEESILSNDGALRIGAVPKKLGLIGSGVIGLEMGSVWRRLGAEVTVLEGLPTFLGAVDQQIAKEAYKAFVKQGLK
IELGVQVGEVKSGKNGVSVAYVDAKGQAQKIDVDKLIVSIGRVANTIGLAPETVGLKLDERGAIVVDDECKTNLPNVWAI
GDVVRGPMLAHKAEEEGVAVAERIAGQHGHVNFNTIPWVIYTNPEIAWVGQTEEQLKAAGRAYRAGTFPFMANGRARALG
DTTGMVKMLADTATDEILGVHIVGPFASELIAECVVAMEFRASSEDIARICHAHPSLSEATKEAALAVDKRTLNF
>Mature_474_residues
SKQFDVIVIGGGPGGYIAAIRAAQLGFNVACIDEWKNAKGGPALGGTCTNVGCIPSKALLQSSEHYEQAGHHFADHGIEV
KGLSLDLAKMVGRKDTVVKQNNDGIVYLFKKNKVTFFHGRASFAAAKDGLYDIKVAGAAEETLSGKHIIVATGSNARALP
GAPFDEESILSNDGALRIGAVPKKLGLIGSGVIGLEMGSVWRRLGAEVTVLEGLPTFLGAVDQQIAKEAYKAFVKQGLKI
ELGVQVGEVKSGKNGVSVAYVDAKGQAQKIDVDKLIVSIGRVANTIGLAPETVGLKLDERGAIVVDDECKTNLPNVWAIG
DVVRGPMLAHKAEEEGVAVAERIAGQHGHVNFNTIPWVIYTNPEIAWVGQTEEQLKAAGRAYRAGTFPFMANGRARALGD
TTGMVKMLADTATDEILGVHIVGPFASELIAECVVAMEFRASSEDIARICHAHPSLSEATKEAALAVDKRTLNF

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=479, Percent_Identity=48.0167014613779, Blast_Score=422, Evalue=1e-118,
Organism=Homo sapiens, GI50301238, Length=470, Percent_Identity=28.0851063829787, Blast_Score=162, Evalue=5e-40,
Organism=Homo sapiens, GI22035672, Length=475, Percent_Identity=28.8421052631579, Blast_Score=126, Evalue=5e-29,
Organism=Homo sapiens, GI33519430, Length=469, Percent_Identity=26.226012793177, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI33519428, Length=469, Percent_Identity=26.226012793177, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI33519426, Length=469, Percent_Identity=26.226012793177, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI148277065, Length=469, Percent_Identity=26.226012793177, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI148277071, Length=469, Percent_Identity=26.226012793177, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI291045266, Length=492, Percent_Identity=24.1869918699187, Blast_Score=102, Evalue=8e-22,
Organism=Homo sapiens, GI291045268, Length=486, Percent_Identity=23.6625514403292, Blast_Score=94, Evalue=3e-19,
Organism=Escherichia coli, GI1786307, Length=460, Percent_Identity=37.3913043478261, Blast_Score=281, Evalue=6e-77,
Organism=Escherichia coli, GI87082354, Length=471, Percent_Identity=29.723991507431, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI87081717, Length=466, Percent_Identity=28.1115879828326, Blast_Score=165, Evalue=5e-42,
Organism=Escherichia coli, GI1789915, Length=467, Percent_Identity=26.7665952890792, Blast_Score=138, Evalue=9e-34,
Organism=Caenorhabditis elegans, GI32565766, Length=472, Percent_Identity=48.3050847457627, Blast_Score=428, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17557007, Length=487, Percent_Identity=27.7207392197125, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI71982272, Length=484, Percent_Identity=27.0661157024793, Blast_Score=124, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI71983419, Length=377, Percent_Identity=30.2387267904509, Blast_Score=118, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI71983429, Length=377, Percent_Identity=30.2387267904509, Blast_Score=118, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI17559934, Length=198, Percent_Identity=33.3333333333333, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6321091, Length=480, Percent_Identity=48.9583333333333, Blast_Score=424, Evalue=1e-119,
Organism=Saccharomyces cerevisiae, GI6325240, Length=481, Percent_Identity=33.6798336798337, Blast_Score=217, Evalue=4e-57,
Organism=Saccharomyces cerevisiae, GI6325166, Length=474, Percent_Identity=27.8481012658228, Blast_Score=162, Evalue=1e-40,
Organism=Drosophila melanogaster, GI21358499, Length=471, Percent_Identity=49.0445859872611, Blast_Score=442, Evalue=1e-124,
Organism=Drosophila melanogaster, GI24640553, Length=492, Percent_Identity=28.2520325203252, Blast_Score=131, Evalue=8e-31,
Organism=Drosophila melanogaster, GI24640549, Length=492, Percent_Identity=28.2520325203252, Blast_Score=131, Evalue=9e-31,
Organism=Drosophila melanogaster, GI24640551, Length=492, Percent_Identity=28.2520325203252, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI17737741, Length=486, Percent_Identity=26.9547325102881, Blast_Score=127, Evalue=1e-29,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 50171; Mature: 50040

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKQFDVIVIGGGPGGYIAAIRAAQLGFNVACIDEWKNAKGGPALGGTCTNVGCIPSKAL
CCCCEEEEEEECCCCCHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHH
LQSSEHYEQAGHHFADHGIEVKGLSLDLAKMVGRKDTVVKQNNDGIVYLFKKNKVTFFHG
HHCHHHHHHHCCHHHHCCEEEEECCCHHHHHCCCCCCEEEECCCCEEEEEECCEEEEEEC
RASFAAAKDGLYDIKVAGAAEETLSGKHIIVATGSNARALPGAPFDEESILSNDGALRIG
CCCHHHHCCCCEEEEEECCHHHHCCCCEEEEEECCCCCCCCCCCCCCHHHHCCCCCEEEC
AVPKKLGLIGSGVIGLEMGSVWRRLGAEVTVLEGLPTFLGAVDQQIAKEAYKAFVKQGLK
CCCHHHCCCCCCEEEEHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCE
IELGVQVGEVKSGKNGVSVAYVDAKGQAQKIDVDKLIVSIGRVANTIGLAPETVGLKLDE
EEEEEEEECCCCCCCCCEEEEEECCCCCEECCHHHHHHHHHHHHHHHCCCCCCCCEEECC
RGAIVVDDECKTNLPNVWAIGDVVRGPMLAHKAEEEGVAVAERIAGQHGHVNFNTIPWVI
CCCEEECCCCCCCCCCEEEEHHHHCCCHHHHCCCCCCHHHHHHHCCCCCCEEECEEEEEE
YTNPEIAWVGQTEEQLKAAGRAYRAGTFPFMANGRARALGDTTGMVKMLADTATDEILGV
EECCEEEEECCCHHHHHHCCCCEECCCCCEEECCCCEECCCCHHHHHHHHHHCCCCEEEE
HIVGPFASELIAECVVAMEFRASSEDIARICHAHPSLSEATKEAALAVDKRTLNF
EEECHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHCCHHCCCC
>Mature Secondary Structure 
SKQFDVIVIGGGPGGYIAAIRAAQLGFNVACIDEWKNAKGGPALGGTCTNVGCIPSKAL
CCCEEEEEEECCCCCHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHH
LQSSEHYEQAGHHFADHGIEVKGLSLDLAKMVGRKDTVVKQNNDGIVYLFKKNKVTFFHG
HHCHHHHHHHCCHHHHCCEEEEECCCHHHHHCCCCCCEEEECCCCEEEEEECCEEEEEEC
RASFAAAKDGLYDIKVAGAAEETLSGKHIIVATGSNARALPGAPFDEESILSNDGALRIG
CCCHHHHCCCCEEEEEECCHHHHCCCCEEEEEECCCCCCCCCCCCCCHHHHCCCCCEEEC
AVPKKLGLIGSGVIGLEMGSVWRRLGAEVTVLEGLPTFLGAVDQQIAKEAYKAFVKQGLK
CCCHHHCCCCCCEEEEHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCE
IELGVQVGEVKSGKNGVSVAYVDAKGQAQKIDVDKLIVSIGRVANTIGLAPETVGLKLDE
EEEEEEEECCCCCCCCCEEEEEECCCCCEECCHHHHHHHHHHHHHHHCCCCCCCCEEECC
RGAIVVDDECKTNLPNVWAIGDVVRGPMLAHKAEEEGVAVAERIAGQHGHVNFNTIPWVI
CCCEEECCCCCCCCCCEEEEHHHHCCCHHHHCCCCCCHHHHHHHCCCCCCEEECEEEEEE
YTNPEIAWVGQTEEQLKAAGRAYRAGTFPFMANGRARALGDTTGMVKMLADTATDEILGV
EECCEEEEECCCHHHHHHCCCCEECCCCCEEECCCCEECCCCHHHHHHHHHHCCCCEEEE
HIVGPFASELIAECVVAMEFRASSEDIARICHAHPSLSEATKEAALAVDKRTLNF
EEECHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8867378 [H]