Click here to switch to the map view.

The map label for this gene is gcvA [H]

Identifier: 91788343

GI number: 91788343

Start: 2594685

End: 2595635

Strand: Direct

Name: gcvA [H]

Synonym: Bpro_2479

Alternate gene names: 91788343

Gene position: 2594685-2595635 (Clockwise)

Preceding gene: 91788339

Following gene: 91788346

Centisome position: 49.9

GC content: 60.04

Gene sequence:

>951_bases
ATGCAAAAACTACCGCCACTCAATTCGATACGCGCTTTCGAAGTCGCTGGCAGGTTGCGCAGCTTTTCGCGCGCTGCCGA
CGAGCTTTCAGTGACTCACGGCGCAGTAAGTCACCAGATCAGGTCGCTGGAAGAGTGGCTGGGGGTGGCCCTGTTTCGGC
GGTTGGGGAAATCTGTGGCGCTGACGGACCAGGGGAGGCTCTACCTCGATTCGGTCAGCCCGGCATTGGCCGCCATTGCG
TCGGCGTCGCGAAGCATCAGCGCGCATGAGGTTCTGCGCGTCAATGCGCTTCCCACGTTCACGATGCGCTGGCTGTTCCC
CAGGCTCGCGCAATTCCGTTCGCAGCATCCCCACATCGAAGTGCAAATCTCCACGGGCCTCGAACCCATGAGCAAACTGC
CCGCCAACATCGATGTGGTGATTCGCCGTGAACCCCAGGAGGTCACGGGCTTGACCAAGGTGCGCATGGTATCCGAATGC
GCCTTCCCAGTCTGTTCACCCACCTTGCTGAGCCAGCTGCCGCTGGCGCACATCACCGACCTGGGTCTTCATACCATCCT
GCAATGCCCAGCCCGGCCCCTGGCGTGGGACGACTGGCTGGCCTTCGCGCACCACAGTCGCGTGGTACCAGCCCAATCAC
TTGAACTCGAGCACCTGTATTTTTGCCTGCAGGCGGCCATAGATGGCATCGGCGTGGCCATGGGCTATTCCCCCTTGGTG
GCCACAGACATCGCCGCCGGCCGCCTCGTCGCGCCCTTCGGTCGCGTGACCCTGGACACTCCAGGCTATTTCCTAATCAC
GCGGACCGAACGCCAGCATGATCCAGTAGTTAAAACTTTTTGTGATTGGCTGCTGGAACAAGGCGCCAATTTCGATGCCG
AGATGAATGCCTTGCTAAAAGCAACACCGATTGACAACGACCTGCATCGCCATCAAACCATGTTGCCGTAG

Upstream 100 bases:

>100_bases
GGCCAATGCGCTTTCTGAAGCCGTGCTGTATTCAATCACAGCGACACGCCCGCTTCAAACGATATAAACTGGCCTGATCT
GCGAATAAAACTAACACTTA

Downstream 100 bases:

>100_bases
CATTGACTTCACTAACCGCGCACCACGCGAGCTGATTGGAAATGAACCGACTTGCGCACCATGGGGGAATCGTACTTTTT
GGGCAAATCGGTGCGCTTGA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MQKLPPLNSIRAFEVAGRLRSFSRAADELSVTHGAVSHQIRSLEEWLGVALFRRLGKSVALTDQGRLYLDSVSPALAAIA
SASRSISAHEVLRVNALPTFTMRWLFPRLAQFRSQHPHIEVQISTGLEPMSKLPANIDVVIRREPQEVTGLTKVRMVSEC
AFPVCSPTLLSQLPLAHITDLGLHTILQCPARPLAWDDWLAFAHHSRVVPAQSLELEHLYFCLQAAIDGIGVAMGYSPLV
ATDIAAGRLVAPFGRVTLDTPGYFLITRTERQHDPVVKTFCDWLLEQGANFDAEMNALLKATPIDNDLHRHQTMLP

Sequences:

>Translated_316_residues
MQKLPPLNSIRAFEVAGRLRSFSRAADELSVTHGAVSHQIRSLEEWLGVALFRRLGKSVALTDQGRLYLDSVSPALAAIA
SASRSISAHEVLRVNALPTFTMRWLFPRLAQFRSQHPHIEVQISTGLEPMSKLPANIDVVIRREPQEVTGLTKVRMVSEC
AFPVCSPTLLSQLPLAHITDLGLHTILQCPARPLAWDDWLAFAHHSRVVPAQSLELEHLYFCLQAAIDGIGVAMGYSPLV
ATDIAAGRLVAPFGRVTLDTPGYFLITRTERQHDPVVKTFCDWLLEQGANFDAEMNALLKATPIDNDLHRHQTMLP
>Mature_316_residues
MQKLPPLNSIRAFEVAGRLRSFSRAADELSVTHGAVSHQIRSLEEWLGVALFRRLGKSVALTDQGRLYLDSVSPALAAIA
SASRSISAHEVLRVNALPTFTMRWLFPRLAQFRSQHPHIEVQISTGLEPMSKLPANIDVVIRREPQEVTGLTKVRMVSEC
AFPVCSPTLLSQLPLAHITDLGLHTILQCPARPLAWDDWLAFAHHSRVVPAQSLELEHLYFCLQAAIDGIGVAMGYSPLV
ATDIAAGRLVAPFGRVTLDTPGYFLITRTERQHDPVVKTFCDWLLEQGANFDAEMNALLKATPIDNDLHRHQTMLP

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=295, Percent_Identity=36.6101694915254, Blast_Score=183, Evalue=1e-47,
Organism=Escherichia coli, GI1786448, Length=289, Percent_Identity=30.1038062283737, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1788706, Length=290, Percent_Identity=32.7586206896552, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1787589, Length=302, Percent_Identity=25.8278145695364, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI157672245, Length=110, Percent_Identity=40.9090909090909, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI145693193, Length=169, Percent_Identity=33.1360946745562, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1786401, Length=275, Percent_Identity=24.3636363636364, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787128, Length=294, Percent_Identity=23.469387755102, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1789639, Length=298, Percent_Identity=23.489932885906, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35013; Mature: 35013

Theoretical pI: Translated: 7.41; Mature: 7.41

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKLPPLNSIRAFEVAGRLRSFSRAADELSVTHGAVSHQIRSLEEWLGVALFRRLGKSVA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
LTDQGRLYLDSVSPALAAIASASRSISAHEVLRVNALPTFTMRWLFPRLAQFRSQHPHIE
EECCCCEEEECCCHHHHHHHHHHCCCCHHHEEEECCCCHHHHHHHHHHHHHHHCCCCCEE
VQISTGLEPMSKLPANIDVVIRREPQEVTGLTKVRMVSECAFPVCSPTLLSQLPLAHITD
EEECCCCCHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHH
LGLHTILQCPARPLAWDDWLAFAHHSRVVPAQSLELEHLYFCLQAAIDGIGVAMGYSPLV
HHHHHHHHCCCCCCCHHHHHHHHHCCCCCCHHHCHHHHHHHHHHHHHHHHHHHHCCCCHH
ATDIAAGRLVAPFGRVTLDTPGYFLITRTERQHDPVVKTFCDWLLEQGANFDAEMNALLK
HHHHCCCCEECCCCCEEECCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHEE
ATPIDNDLHRHQTMLP
ECCCCCHHHHHHCCCC
>Mature Secondary Structure
MQKLPPLNSIRAFEVAGRLRSFSRAADELSVTHGAVSHQIRSLEEWLGVALFRRLGKSVA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
LTDQGRLYLDSVSPALAAIASASRSISAHEVLRVNALPTFTMRWLFPRLAQFRSQHPHIE
EECCCCEEEECCCHHHHHHHHHHCCCCHHHEEEECCCCHHHHHHHHHHHHHHHCCCCCEE
VQISTGLEPMSKLPANIDVVIRREPQEVTGLTKVRMVSECAFPVCSPTLLSQLPLAHITD
EEECCCCCHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHH
LGLHTILQCPARPLAWDDWLAFAHHSRVVPAQSLELEHLYFCLQAAIDGIGVAMGYSPLV
HHHHHHHHCCCCCCCHHHHHHHHHCCCCCCHHHCHHHHHHHHHHHHHHHHHHHHCCCCHH
ATDIAAGRLVAPFGRVTLDTPGYFLITRTERQHDPVVKTFCDWLLEQGANFDAEMNALLK
HHHHCCCCEECCCCCEEECCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHEE
ATPIDNDLHRHQTMLP
ECCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]