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The map label for this gene is fabI [H]

Identifier: 91788284

GI number: 91788284

Start: 2517450

End: 2518247

Strand: Reverse

Name: fabI [H]

Synonym: Bpro_2419

Alternate gene names: 91788284

Gene position: 2518247-2517450 (Counterclockwise)

Preceding gene: 91788297

Following gene: 91788281

Centisome position: 48.43

GC content: 62.03

Gene sequence:

>798_bases
ATGGGTTTTCTTACCGGCAAAAAACTGTTGATCACGGGCGTGTTGTCCAACCGGTCCATTGCCTACGGCATAGCCAAGGC
GTGCCACGCACAAGGCGCCGAACTGGCCTTCAGCTATGTCGGGGAGCGCTTCAAGGACCGCATCACGGAGTTTGCCGCCG
ATTTTGGCTCCAAACTCATCTTTGACTGCGACGTCGGCGACGACGCCCAAATCGAGAAGCTGTTTGCCGACCTGGCCAAG
ACCTGGCCGACGTTTGACGGCTTTGTTCACAGCATTGGCTACGCACCGCGCGAAGCCATTGCCGGCGATTTCCTCGACGG
CCTCACCCGCGAGGGCTTCAAGATTGCCCACGACATCAGCGCCTACAGCTTTCCGGCCATGGCCAAGGCGGCGCTGCCCT
ACCTCAACAACAAGTCGGCGCTGCTGACCCTGACCTACCTGGGCGCGGTACGTACCGTGCCCAACTACAACACCATGGGG
CTGGCCAAGGCCAGCCTGGAGGCCTCGGTGCGCTACCTGGCCGAATCGCTGGGCCCCAAGGGCATGCGCGTCAACGGCAT
CAGCGCCGGCCCGATCAAGACCCTGGCCGCCAGCGGCATCAAGGGCTTCGGCAAGATTCTGAGCGTCGTCGCCGAAGCCT
CACCCATTCGCCGCAACGTAACGATTGAAGACGTCGGCAATGTCGCTGCGTTCTTGCTGAGTGACCTGGCTGGTGGCGTG
AGCGCCGAAATCACCTACGTGGATGGTGGTTTCAGCCACGTGGTGGGCGGCATCGCCGAGCCAACCGAAGCAGGCTGA

Upstream 100 bases:

>100_bases
CACGTCGGCAACGGTTGGCGGCCAGCAGGTAAGCCAGCTGCCCCATTCAATGCAACCAACCGCTATCGGCCGATCCACTC
GTACACACTGGAGCACAAGC

Downstream 100 bases:

>100_bases
ACGCCACGACGGCGGGCTTGGCAGCCCGACTTGCTATCATTTTGATGGCAAACGGTGCCCACGCATGTAAAGCTGAGGGC
CAAATCTACTTGTCCTTGAC

Product: enoyl-[acyl-carrier-protein] reductase

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase [H]

Number of amino acids: Translated: 265; Mature: 264

Protein sequence:

>265_residues
MGFLTGKKLLITGVLSNRSIAYGIAKACHAQGAELAFSYVGERFKDRITEFAADFGSKLIFDCDVGDDAQIEKLFADLAK
TWPTFDGFVHSIGYAPREAIAGDFLDGLTREGFKIAHDISAYSFPAMAKAALPYLNNKSALLTLTYLGAVRTVPNYNTMG
LAKASLEASVRYLAESLGPKGMRVNGISAGPIKTLAASGIKGFGKILSVVAEASPIRRNVTIEDVGNVAAFLLSDLAGGV
SAEITYVDGGFSHVVGGIAEPTEAG

Sequences:

>Translated_265_residues
MGFLTGKKLLITGVLSNRSIAYGIAKACHAQGAELAFSYVGERFKDRITEFAADFGSKLIFDCDVGDDAQIEKLFADLAK
TWPTFDGFVHSIGYAPREAIAGDFLDGLTREGFKIAHDISAYSFPAMAKAALPYLNNKSALLTLTYLGAVRTVPNYNTMG
LAKASLEASVRYLAESLGPKGMRVNGISAGPIKTLAASGIKGFGKILSVVAEASPIRRNVTIEDVGNVAAFLLSDLAGGV
SAEITYVDGGFSHVVGGIAEPTEAG
>Mature_264_residues
GFLTGKKLLITGVLSNRSIAYGIAKACHAQGAELAFSYVGERFKDRITEFAADFGSKLIFDCDVGDDAQIEKLFADLAKT
WPTFDGFVHSIGYAPREAIAGDFLDGLTREGFKIAHDISAYSFPAMAKAALPYLNNKSALLTLTYLGAVRTVPNYNTMGL
AKASLEASVRYLAESLGPKGMRVNGISAGPIKTLAASGIKGFGKILSVVAEASPIRRNVTIEDVGNVAAFLLSDLAGGVS
AEITYVDGGFSHVVGGIAEPTEAG

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787545, Length=252, Percent_Identity=64.2857142857143, Blast_Score=344, Evalue=3e-96,
Organism=Escherichia coli, GI87082100, Length=259, Percent_Identity=26.6409266409266, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1789378, Length=253, Percent_Identity=27.2727272727273, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 27853; Mature: 27722

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFLTGKKLLITGVLSNRSIAYGIAKACHAQGAELAFSYVGERFKDRITEFAADFGSKLI
CCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEE
FDCDVGDDAQIEKLFADLAKTWPTFDGFVHSIGYAPREAIAGDFLDGLTREGFKIAHDIS
EECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHH
AYSFPAMAKAALPYLNNKSALLTLTYLGAVRTVPNYNTMGLAKASLEASVRYLAESLGPK
HHCCHHHHHHHCCCCCCCCCEEEHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
GMRVNGISAGPIKTLAASGIKGFGKILSVVAEASPIRRNVTIEDVGNVAAFLLSDLAGGV
CEEECCCCCCHHHHHHHHCHHHHHHHHHHHHHCCCHHCCCCHHHHHHHHHHHHHHHCCCC
SAEITYVDGGFSHVVGGIAEPTEAG
EEEEEEECCCHHHHHCCCCCCCCCC
>Mature Secondary Structure 
GFLTGKKLLITGVLSNRSIAYGIAKACHAQGAELAFSYVGERFKDRITEFAADFGSKLI
CCCCCCCEEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEE
FDCDVGDDAQIEKLFADLAKTWPTFDGFVHSIGYAPREAIAGDFLDGLTREGFKIAHDIS
EECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHH
AYSFPAMAKAALPYLNNKSALLTLTYLGAVRTVPNYNTMGLAKASLEASVRYLAESLGPK
HHCCHHHHHHHCCCCCCCCCEEEHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
GMRVNGISAGPIKTLAASGIKGFGKILSVVAEASPIRRNVTIEDVGNVAAFLLSDLAGGV
CEEECCCCCCHHHHHHHHCHHHHHHHHHHHHHCCCHHCCCCHHHHHHHHHHHHHHHCCCC
SAEITYVDGGFSHVVGGIAEPTEAG
EEEEEEECCCHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]