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The map label for this gene is pleD [H]

Identifier: 91788256

GI number: 91788256

Start: 2483550

End: 2484029

Strand: Reverse

Name: pleD [H]

Synonym: Bpro_2389

Alternate gene names: 91788256

Gene position: 2484029-2483550 (Counterclockwise)

Preceding gene: 91788257

Following gene: 91788251

Centisome position: 47.77

GC content: 60.0

Gene sequence:

>480_bases
ATGAACACCGAATTCGACATTCTGAACGCCAGCATCCTGGTGGTGGACGACCAGGCCGCCAATGTGCAGCTGCTGGAGCA
GCTTCTGAGCAACACCGGCTACACCCGCGTCCGCTCCACCATGAATCCACTGGAGGTGGCCGGTCTGCACGCGGAGCACC
GCTATGACCTGATCCTGCTGGACCTGAAGATGCCCGGCATGGACGGCTTCCAGGTGATGCAGGAGCTCAAGAAAAACACC
AGCGACGACTACCTGCCCGTCATCGTCCTGACCGCGGAGCCGGGCCACAAGCTGCGCGCCCTGGAGGCCGGCGCCAAGGA
CTTCATCAGCAAGCCCTTTGACCTGGTTGAAGTCAAGACGCGCATCCACAACATGCTGGAAGTCCGTCTGCTGTATAAAA
AACTGGACAACTACAGCAAGCTGCTGGAGCACGCCGTGCACCAGGGCACGGTCGAGATCGACGAAGCGCGAAGGCAATAA

Upstream 100 bases:

>100_bases
CCAAACCCATCCGGATCAATGAATTCATGGACACCCTGGACCTGGCGCTGAAGTTTTCCAAAACAAAGTCCCCACGCCCT
TCCGTACCGGAGAAAACCGC

Downstream 100 bases:

>100_bases
ACGCCTGGTTCTTGGTGGGCGGGCCGGACGCCGGACGGCTCAATGAAAGTCGCGGCTGGAGGTGCCCAGCCGCCCCAGCA
GCGGCGTGAGGTCTTCCAGC

Product: response regulator receiver domain-containing protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MNTEFDILNASILVVDDQAANVQLLEQLLSNTGYTRVRSTMNPLEVAGLHAEHRYDLILLDLKMPGMDGFQVMQELKKNT
SDDYLPVIVLTAEPGHKLRALEAGAKDFISKPFDLVEVKTRIHNMLEVRLLYKKLDNYSKLLEHAVHQGTVEIDEARRQ

Sequences:

>Translated_159_residues
MNTEFDILNASILVVDDQAANVQLLEQLLSNTGYTRVRSTMNPLEVAGLHAEHRYDLILLDLKMPGMDGFQVMQELKKNT
SDDYLPVIVLTAEPGHKLRALEAGAKDFISKPFDLVEVKTRIHNMLEVRLLYKKLDNYSKLLEHAVHQGTVEIDEARRQ
>Mature_159_residues
MNTEFDILNASILVVDDQAANVQLLEQLLSNTGYTRVRSTMNPLEVAGLHAEHRYDLILLDLKMPGMDGFQVMQELKKNT
SDDYLPVIVLTAEPGHKLRALEAGAKDFISKPFDLVEVKTRIHNMLEVRLLYKKLDNYSKLLEHAVHQGTVEIDEARRQ

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1789809, Length=120, Percent_Identity=36.6666666666667, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI87082012, Length=118, Percent_Identity=33.0508474576271, Blast_Score=67, Evalue=5e-13,
Organism=Escherichia coli, GI1786599, Length=117, Percent_Identity=36.7521367521368, Blast_Score=66, Evalue=9e-13,
Organism=Escherichia coli, GI1786911, Length=124, Percent_Identity=33.8709677419355, Blast_Score=62, Evalue=2e-11,
Organism=Escherichia coli, GI1788550, Length=142, Percent_Identity=28.169014084507, Blast_Score=61, Evalue=3e-11,
Organism=Escherichia coli, GI1790299, Length=109, Percent_Identity=34.8623853211009, Blast_Score=61, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 18099; Mature: 18099

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTEFDILNASILVVDDQAANVQLLEQLLSNTGYTRVRSTMNPLEVAGLHAEHRYDLILL
CCCCCCEECEEEEEEECCCCCHHHHHHHHHCCCHHHHHHCCCHHHHHCCCCCCCCCEEEE
DLKMPGMDGFQVMQELKKNTSDDYLPVIVLTAEPGHKLRALEAGAKDFISKPFDLVEVKT
EEECCCCCHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHCHHHHHHCCHHHHHHHH
RIHNMLEVRLLYKKLDNYSKLLEHAVHQGTVEIDEARRQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHCCC
>Mature Secondary Structure
MNTEFDILNASILVVDDQAANVQLLEQLLSNTGYTRVRSTMNPLEVAGLHAEHRYDLILL
CCCCCCEECEEEEEEECCCCCHHHHHHHHHCCCHHHHHHCCCHHHHHCCCCCCCCCEEEE
DLKMPGMDGFQVMQELKKNTSDDYLPVIVLTAEPGHKLRALEAGAKDFISKPFDLVEVKT
EEECCCCCHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHCHHHHHHCCHHHHHHHH
RIHNMLEVRLLYKKLDNYSKLLEHAVHQGTVEIDEARRQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA