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The map label for this gene is tcmA [H]

Identifier: 91788237

GI number: 91788237

Start: 2463431

End: 2464993

Strand: Reverse

Name: tcmA [H]

Synonym: Bpro_2370

Alternate gene names: 91788237

Gene position: 2464993-2463431 (Counterclockwise)

Preceding gene: 91788239

Following gene: 91788233

Centisome position: 47.4

GC content: 67.31

Gene sequence:

>1563_bases
ATGCTGGGTAACGCGCAATCTCCTTGTGACAAAGGAGTCATTCAGGCACAACGCAGCTTGCCCAGCACTTCCTTGGCCCC
TTCCCTACTGACTTCGCAGCCCTCTTCTGCCGGTGCAGACGCCGCCGCCCGAAAAGGCTATGCCCTGGCCGCCACCGTCC
TCGGTTCGTCCATGGCCTTCATCGACGGCTCGGTCGTCAACATTGCCCTGCCCGCCATCCAGCGCGACTTTGCCAGTAGC
GCCGGCGCCAGCCTGGCGGCCATGCAGTGGGTCATCAACGCCTATCTGCTGGCGCTGGGTTCGCTAGTGCTGGTGGGCGG
CTCGCTGGGGGACAGGTTCGGGCGGCGCAGCATTTTCATCACCGGCATTGCGCTTTTTACCGCCGCGTCGGCGGCCTGCG
GGCTGGCCTCCGGCACGCTGGCCCTGATTGCCGCGCGGGCGCTGCAGGGCGTGGGCGCGGCGCTGCTGGTGCCAGCCAGC
CTGGCCATTATTGGCAACGTATTTGAAGGCGACGCACGCGGCAAGGCGATTGGCGCTTGGGCGGCGTGGGCGTCCATCAC
GGGTGCGGCGGGTCCGGTGCTGGGCGGCTGGCTGGTCGATGCAGTGTCGTGGCGCGCCATCTTTTTTCTGAACGTACCGC
TGGCCGTGGCCACCATCTGGCTGGCCGTCTATGCCGTGCCCGACAGCCGCGACCCCGATGCGCCGCGGCATTTCGACTGG
CTCGGTGTGTTGCTGGTGGCCAGCGGGCTGGGTGCCCTCACCTATGGGCTGACACTGGCGCCCGAGCGCGGATGGGGCCA
CGCGAGCGTGCTGTGGCCCCTGGTGTTGGGCGTAGTCGGGCTGGCTGCTTTTGCCGTGGCGCAGGCGCGGGTGCGTGCGC
CCATGATGCCGCCGGCGCTTTTCAGGTCGCGCGACTTTGTCGGTGCCAATCTGCTGACACTGCTGCTGTACTTTGCGCTG
GTCGGCGTGCTGTTTTTTCTGCCCTTTGTGCTGATTGGCGCCTTCCGCTATTCGGCCACCGCAGCGGGTGCCACGCTGCT
GCCCTTCTCCATCATTTTGGGCTTGCTGTCCTCTGCGGCCGGCAAGGCCATGGATCGTTTTGGCGCCAGGCTCATGCTTA
TCTGCGGTCCGGTGATCGCCGCTGCCGGCCTGGCGTTGATGGCGCTGCCGGGTGTGGGCTGGCCTTACTGGGCGGGCTTT
TTTCCGGCCATGGTGGTGCTGGGCCTGGGCATGACGATTGCCATTGCACCGCTCACCACCACCGTGATGAACGCGGTGTC
GCCGGCCCACGCCGGGGTGGCCTCGGGCGTGAACAACGCGGTGGCGCGGGTGGCGGGCTTGCTCGCAGTGGCCGTGCTGG
GTGTGGTGTTTGCCGCTGCCTTGGGCGGGCCCCTGGACGGCGCACCCGCGACTACGCTGATCCACGCGTTTCGCTGGGTG
GTGCTGGCAGCCGTGCTGTGCGCGCTGGCCTCGGCAGCCTGTGCGGCCATGTTTTTAACGCCTGCACCCGCGCCAGTTAC
AAAACGCAAGCCTGTCAGAACCCGGCCAGCACCGCCCCCTTGA

Upstream 100 bases:

>100_bases
CTTGCCGTGCTACAGCACTGTCTGCGGCGGCACGCCTAGCCAGGACCGCTGCGCTGGGTTTTGTTAGCGGCTCTTAACTC
CTTAACCAACGAAAAAGTCC

Downstream 100 bases:

>100_bases
ACTCAGTCTGGATGAACTTGCGGATTTCTTCAGAGTGGTAGGCCTTGACCAGCTTGGCCACCCAGGGCTTGTCCTTGTCT
TGCTCGCGCACAGCCAGCAG

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 520; Mature: 520

Protein sequence:

>520_residues
MLGNAQSPCDKGVIQAQRSLPSTSLAPSLLTSQPSSAGADAAARKGYALAATVLGSSMAFIDGSVVNIALPAIQRDFASS
AGASLAAMQWVINAYLLALGSLVLVGGSLGDRFGRRSIFITGIALFTAASAACGLASGTLALIAARALQGVGAALLVPAS
LAIIGNVFEGDARGKAIGAWAAWASITGAAGPVLGGWLVDAVSWRAIFFLNVPLAVATIWLAVYAVPDSRDPDAPRHFDW
LGVLLVASGLGALTYGLTLAPERGWGHASVLWPLVLGVVGLAAFAVAQARVRAPMMPPALFRSRDFVGANLLTLLLYFAL
VGVLFFLPFVLIGAFRYSATAAGATLLPFSIILGLLSSAAGKAMDRFGARLMLICGPVIAAAGLALMALPGVGWPYWAGF
FPAMVVLGLGMTIAIAPLTTTVMNAVSPAHAGVASGVNNAVARVAGLLAVAVLGVVFAAALGGPLDGAPATTLIHAFRWV
VLAAVLCALASAACAAMFLTPAPAPVTKRKPVRTRPAPPP

Sequences:

>Translated_520_residues
MLGNAQSPCDKGVIQAQRSLPSTSLAPSLLTSQPSSAGADAAARKGYALAATVLGSSMAFIDGSVVNIALPAIQRDFASS
AGASLAAMQWVINAYLLALGSLVLVGGSLGDRFGRRSIFITGIALFTAASAACGLASGTLALIAARALQGVGAALLVPAS
LAIIGNVFEGDARGKAIGAWAAWASITGAAGPVLGGWLVDAVSWRAIFFLNVPLAVATIWLAVYAVPDSRDPDAPRHFDW
LGVLLVASGLGALTYGLTLAPERGWGHASVLWPLVLGVVGLAAFAVAQARVRAPMMPPALFRSRDFVGANLLTLLLYFAL
VGVLFFLPFVLIGAFRYSATAAGATLLPFSIILGLLSSAAGKAMDRFGARLMLICGPVIAAAGLALMALPGVGWPYWAGF
FPAMVVLGLGMTIAIAPLTTTVMNAVSPAHAGVASGVNNAVARVAGLLAVAVLGVVFAAALGGPLDGAPATTLIHAFRWV
VLAAVLCALASAACAAMFLTPAPAPVTKRKPVRTRPAPPP
>Mature_520_residues
MLGNAQSPCDKGVIQAQRSLPSTSLAPSLLTSQPSSAGADAAARKGYALAATVLGSSMAFIDGSVVNIALPAIQRDFASS
AGASLAAMQWVINAYLLALGSLVLVGGSLGDRFGRRSIFITGIALFTAASAACGLASGTLALIAARALQGVGAALLVPAS
LAIIGNVFEGDARGKAIGAWAAWASITGAAGPVLGGWLVDAVSWRAIFFLNVPLAVATIWLAVYAVPDSRDPDAPRHFDW
LGVLLVASGLGALTYGLTLAPERGWGHASVLWPLVLGVVGLAAFAVAQARVRAPMMPPALFRSRDFVGANLLTLLLYFAL
VGVLFFLPFVLIGAFRYSATAAGATLLPFSIILGLLSSAAGKAMDRFGARLMLICGPVIAAAGLALMALPGVGWPYWAGF
FPAMVVLGLGMTIAIAPLTTTVMNAVSPAHAGVASGVNNAVARVAGLLAVAVLGVVFAAALGGPLDGAPATTLIHAFRWV
VLAAVLCALASAACAAMFLTPAPAPVTKRKPVRTRPAPPP

Specific function: Resistance to tetracenomycin C by an active tetracenomycin C efflux system which is probably energized by transmembrane electrochemical gradients [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=470, Percent_Identity=28.5106382978723, Blast_Score=138, Evalue=1e-33,
Organism=Escherichia coli, GI1789042, Length=408, Percent_Identity=27.2058823529412, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI87081983, Length=401, Percent_Identity=28.6783042394015, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI1788710, Length=321, Percent_Identity=26.4797507788162, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1788392, Length=362, Percent_Identity=30.1104972375691, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1790146, Length=175, Percent_Identity=28, Blast_Score=74, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6323520, Length=415, Percent_Identity=26.0240963855422, Blast_Score=98, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6323935, Length=404, Percent_Identity=23.5148514851485, Blast_Score=83, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324954, Length=406, Percent_Identity=23.6453201970443, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6319770, Length=411, Percent_Identity=26.0340632603406, Blast_Score=68, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 52600; Mature: 52600

Theoretical pI: Translated: 10.62; Mature: 10.62

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGNAQSPCDKGVIQAQRSLPSTSLAPSLLTSQPSSAGADAAARKGYALAATVLGSSMAF
CCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCHHHHCCHHHHHHHHCCHHHH
IDGSVVNIALPAIQRDFASSAGASLAAMQWVINAYLLALGSLVLVGGSLGDRFGRRSIFI
HCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHH
TGIALFTAASAACGLASGTLALIAARALQGVGAALLVPASLAIIGNVFEGDARGKAIGAW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCHHHH
AAWASITGAAGPVLGGWLVDAVSWRAIFFLNVPLAVATIWLAVYAVPDSRDPDAPRHFDW
HHHHHHCCCCCHHHHHHHHHHHHHHHHEEEECHHHHHHHHHHHHCCCCCCCCCCCCHHHH
LGVLLVASGLGALTYGLTLAPERGWGHASVLWPLVLGVVGLAAFAVAQARVRAPMMPPAL
HHHHHHHHCHHHHHHCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
FRSRDFVGANLLTLLLYFALVGVLFFLPFVLIGAFRYSATAAGATLLPFSIILGLLSSAA
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
GKAMDRFGARLMLICGPVIAAAGLALMALPGVGWPYWAGFFPAMVVLGLGMTIAIAPLTT
HHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHH
TVMNAVSPAHAGVASGVNNAVARVAGLLAVAVLGVVFAAALGGPLDGAPATTLIHAFRWV
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
VLAAVLCALASAACAAMFLTPAPAPVTKRKPVRTRPAPPP
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLGNAQSPCDKGVIQAQRSLPSTSLAPSLLTSQPSSAGADAAARKGYALAATVLGSSMAF
CCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCHHHHCCHHHHHHHHCCHHHH
IDGSVVNIALPAIQRDFASSAGASLAAMQWVINAYLLALGSLVLVGGSLGDRFGRRSIFI
HCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHH
TGIALFTAASAACGLASGTLALIAARALQGVGAALLVPASLAIIGNVFEGDARGKAIGAW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCHHHH
AAWASITGAAGPVLGGWLVDAVSWRAIFFLNVPLAVATIWLAVYAVPDSRDPDAPRHFDW
HHHHHHCCCCCHHHHHHHHHHHHHHHHEEEECHHHHHHHHHHHHCCCCCCCCCCCCHHHH
LGVLLVASGLGALTYGLTLAPERGWGHASVLWPLVLGVVGLAAFAVAQARVRAPMMPPAL
HHHHHHHHCHHHHHHCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
FRSRDFVGANLLTLLLYFALVGVLFFLPFVLIGAFRYSATAAGATLLPFSIILGLLSSAA
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
GKAMDRFGARLMLICGPVIAAAGLALMALPGVGWPYWAGFFPAMVVLGLGMTIAIAPLTT
HHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHH
TVMNAVSPAHAGVASGVNNAVARVAGLLAVAVLGVVFAAALGGPLDGAPATTLIHAFRWV
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
VLAAVLCALASAACAAMFLTPAPAPVTKRKPVRTRPAPPP
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1592819 [H]