Click here to switch to the map view.

The map label for this gene is mltD [H]

Identifier: 91788148

GI number: 91788148

Start: 2368712

End: 2370115

Strand: Reverse

Name: mltD [H]

Synonym: Bpro_2278

Alternate gene names: 91788148

Gene position: 2370115-2368712 (Counterclockwise)

Preceding gene: 91788155

Following gene: 91788134

Centisome position: 45.58

GC content: 61.97

Gene sequence:

>1404_bases
ATGACGGGGCAAGCGCCTGCGCCGGCGCAGGAGCCCGACCTCAAGCCGCTGATCCCCAACGGTCCACTGCAGGCCATCAC
GCCGGGCCAAGCCGGCTCGCACCAGATTGCGTCCACCGAGCCGCCCAGGGAGTTGTGGGACCGGATTCGCCGCGGCTTTG
CCATGCCCAACCTGCAAAACGAACTGGTGACTGACCGTGAGCAGTGGTATGCCAGCCGGCCCGACTACATCCAGCGCATG
ACCGAGCGCTCCAGCAAGTATCTCTTTCATATTGTTGAAGAGCTGGAGCGCCGCCAGATGCCTACCGAACTGGCCCTGTT
GCCCTTCATTGAGAGCGCCTTTAACCCCCAGGCCGTTTCCAGCGCCAAGGCCGCCGGGATGTGGCAGTTCATGCCCGCCA
CGGGCAAGTACTTTGACCTGAAGCAAAACGCTTTTCGCGATGACCGCCGCGATGTTCTGGCCTCGACCCGGGCGGCGCTG
GATTACCTGCAAAAGCTCTACGGCATGTTTGGTGACTGGCACCTGGCGCTGGCCGCCTACAACTGGGGTGAAGGCAGCGT
GGGCCGCGCGATCGCCAAAAACCAGAGGGCCGGCCTGGGCACCAGTTACCTCGACCTCAACATGCCGGCGGAGACCCGGC
TTTACGTGCCCAAGCTGCAGGCCGTGAAAAACATCGTGGCGAACCCGCAGGGGTTCGGCACGGAACTGCCGCTGATCGAA
AACCACCCCTATTTCCAGCAGGTGATGATCACGCGCGACATCGATGTGACCCTGGCCGCACGGCTGGCCGATGTCAAGCT
TGAAGACTTCAAGGCGCTCAATCCCTCGGCCCATCGCCCGGTCATCATCGCGGCTGGCACGCCCCACATCTTGCTGCCCT
GGGACAATGCCATCGTCTTTCAACGCAATTTTGACGCCTACACCCAGGGCCAATATGCCAGCTGGACCGCATGGACAGCC
CCGACAACCATGAGTCCGGCCGAGGCAGCGCGACGCACCGGAATGAGCGAGACCGATCTGCGCAGCGTGAACAACATACC
ACCGCGCATGTTGATCAAGGCAGGCTCCACCCTGCTGGTGCCGCGATCGGCAAAAATGGAAAATGACGTGACAGGCCACC
TGGCCGACAGCGCTCAGGTTTCATTGGCGCCTGAAATGGTCACGCGGCGTACCACCGTCAAGGCACGCAAAGGTGAATCA
GTCGCCACCATCGCCAGGCGCTACAAATTGAGCGCGGCCAGCGTGGCTGAATGGAATGGCGTCAGTGCTTCTGCCGCCTT
CAAGCTGGGGCAACAGGTGGTTGTGTTCTTGCCGGTCAAGAGCCGTGCTTCCTCCGGGCGCAGCAATGTCAAAGCCGTCA
AGCGCAGCACCAGCAAGACCATTGTCAAGTCGCGAAGACGCTGA

Upstream 100 bases:

>100_bases
CATCCTGCACTCCCGCCTCGTTTCGCTTGCGTTCTTGCTCGTGGTCGCAGTGCTGGCCGGTTGCGCCAGCCCACCGGGCA
TGCCGGGTGATCCGGTGCTC

Downstream 100 bases:

>100_bases
CCGAGCCCGGAAAACAGCCGCCATCCTGGCTCAAGCGCTGGTGGCCTGAAGATTGACCGCTGCAGCCCTCACCGACGCAT
CGTCCCTGTGACGCGGCGCA

Product: lytic transglycosylase catalytic subunit

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase D; Regulatory protein dniR [H]

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MTGQAPAPAQEPDLKPLIPNGPLQAITPGQAGSHQIASTEPPRELWDRIRRGFAMPNLQNELVTDREQWYASRPDYIQRM
TERSSKYLFHIVEELERRQMPTELALLPFIESAFNPQAVSSAKAAGMWQFMPATGKYFDLKQNAFRDDRRDVLASTRAAL
DYLQKLYGMFGDWHLALAAYNWGEGSVGRAIAKNQRAGLGTSYLDLNMPAETRLYVPKLQAVKNIVANPQGFGTELPLIE
NHPYFQQVMITRDIDVTLAARLADVKLEDFKALNPSAHRPVIIAAGTPHILLPWDNAIVFQRNFDAYTQGQYASWTAWTA
PTTMSPAEAARRTGMSETDLRSVNNIPPRMLIKAGSTLLVPRSAKMENDVTGHLADSAQVSLAPEMVTRRTTVKARKGES
VATIARRYKLSAASVAEWNGVSASAAFKLGQQVVVFLPVKSRASSGRSNVKAVKRSTSKTIVKSRRR

Sequences:

>Translated_467_residues
MTGQAPAPAQEPDLKPLIPNGPLQAITPGQAGSHQIASTEPPRELWDRIRRGFAMPNLQNELVTDREQWYASRPDYIQRM
TERSSKYLFHIVEELERRQMPTELALLPFIESAFNPQAVSSAKAAGMWQFMPATGKYFDLKQNAFRDDRRDVLASTRAAL
DYLQKLYGMFGDWHLALAAYNWGEGSVGRAIAKNQRAGLGTSYLDLNMPAETRLYVPKLQAVKNIVANPQGFGTELPLIE
NHPYFQQVMITRDIDVTLAARLADVKLEDFKALNPSAHRPVIIAAGTPHILLPWDNAIVFQRNFDAYTQGQYASWTAWTA
PTTMSPAEAARRTGMSETDLRSVNNIPPRMLIKAGSTLLVPRSAKMENDVTGHLADSAQVSLAPEMVTRRTTVKARKGES
VATIARRYKLSAASVAEWNGVSASAAFKLGQQVVVFLPVKSRASSGRSNVKAVKRSTSKTIVKSRRR
>Mature_466_residues
TGQAPAPAQEPDLKPLIPNGPLQAITPGQAGSHQIASTEPPRELWDRIRRGFAMPNLQNELVTDREQWYASRPDYIQRMT
ERSSKYLFHIVEELERRQMPTELALLPFIESAFNPQAVSSAKAAGMWQFMPATGKYFDLKQNAFRDDRRDVLASTRAALD
YLQKLYGMFGDWHLALAAYNWGEGSVGRAIAKNQRAGLGTSYLDLNMPAETRLYVPKLQAVKNIVANPQGFGTELPLIEN
HPYFQQVMITRDIDVTLAARLADVKLEDFKALNPSAHRPVIIAAGTPHILLPWDNAIVFQRNFDAYTQGQYASWTAWTAP
TTMSPAEAARRTGMSETDLRSVNNIPPRMLIKAGSTLLVPRSAKMENDVTGHLADSAQVSLAPEMVTRRTTVKARKGESV
ATIARRYKLSAASVAEWNGVSASAAFKLGQQVVVFLPVKSRASSGRSNVKAVKRSTSKTIVKSRRR

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=414, Percent_Identity=30.6763285024155, Blast_Score=204, Evalue=1e-53,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR010511
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01476 LysM; PF06474 MLTD_N; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 51818; Mature: 51687

Theoretical pI: Translated: 10.45; Mature: 10.45

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGQAPAPAQEPDLKPLIPNGPLQAITPGQAGSHQIASTEPPRELWDRIRRGFAMPNLQN
CCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHH
ELVTDREQWYASRPDYIQRMTERSSKYLFHIVEELERRQMPTELALLPFIESAFNPQAVS
HHHCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHH
SAKAAGMWQFMPATGKYFDLKQNAFRDDRRDVLASTRAALDYLQKLYGMFGDWHLALAAY
HHHHCCCEEEECCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEE
NWGEGSVGRAIAKNQRAGLGTSYLDLNMPAETRLYVPKLQAVKNIVANPQGFGTELPLIE
ECCCCCHHHHHHHCCCCCCCCEEEEECCCCCCEEECHHHHHHHHHHCCCCCCCCCCCEEC
NHPYFQQVMITRDIDVTLAARLADVKLEDFKALNPSAHRPVIIAAGTPHILLPWDNAIVF
CCCHHHHEEEECCCCCEEHHHHHHCCHHHHHCCCCCCCCCEEEECCCCEEEEECCCEEEE
QRNFDAYTQGQYASWTAWTAPTTMSPAEAARRTGMSETDLRSVNNIPPRMLIKAGSTLLV
EECCCCCCCCCCCCEEEECCCCCCCHHHHHHHCCCCHHHHHHHCCCCHHHEEECCCEEEE
PRSAKMENDVTGHLADSAQVSLAPEMVTRRTTVKARKGESVATIARRYKLSAASVAEWNG
CCCCCCCCCCCCEECCCCCEEECHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHCCC
VSASAAFKLGQQVVVFLPVKSRASSGRSNVKAVKRSTSKTIVKSRRR
CCHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TGQAPAPAQEPDLKPLIPNGPLQAITPGQAGSHQIASTEPPRELWDRIRRGFAMPNLQN
CCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHH
ELVTDREQWYASRPDYIQRMTERSSKYLFHIVEELERRQMPTELALLPFIESAFNPQAVS
HHHCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHH
SAKAAGMWQFMPATGKYFDLKQNAFRDDRRDVLASTRAALDYLQKLYGMFGDWHLALAAY
HHHHCCCEEEECCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEE
NWGEGSVGRAIAKNQRAGLGTSYLDLNMPAETRLYVPKLQAVKNIVANPQGFGTELPLIE
ECCCCCHHHHHHHCCCCCCCCEEEEECCCCCCEEECHHHHHHHHHHCCCCCCCCCCCEEC
NHPYFQQVMITRDIDVTLAARLADVKLEDFKALNPSAHRPVIIAAGTPHILLPWDNAIVF
CCCHHHHEEEECCCCCEEHHHHHHCCHHHHHCCCCCCCCCEEEECCCCEEEEECCCEEEE
QRNFDAYTQGQYASWTAWTAPTTMSPAEAARRTGMSETDLRSVNNIPPRMLIKAGSTLLV
EECCCCCCCCCCCCEEEECCCCCCCHHHHHHHCCCCHHHHHHHCCCCHHHEEECCCEEEE
PRSAKMENDVTGHLADSAQVSLAPEMVTRRTTVKARKGESVATIARRYKLSAASVAEWNG
CCCCCCCCCCCCEECCCCCEEECHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHCCC
VSASAAFKLGQQVVVFLPVKSRASSGRSNVKAVKRSTSKTIVKSRRR
CCHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]