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The map label for this gene is rnfB [H]

Identifier: 91788043

GI number: 91788043

Start: 2264096

End: 2264761

Strand: Reverse

Name: rnfB [H]

Synonym: Bpro_2170

Alternate gene names: 91788043

Gene position: 2264761-2264096 (Counterclockwise)

Preceding gene: 91788044

Following gene: 91788033

Centisome position: 43.55

GC content: 62.76

Gene sequence:

>666_bases
ATGAACGATTTAACTGCACGCCTCCACGCTGCCTTGCCGCAGACGCAATGCACCCGTTGCGGCTATCCTGACTGCGCGGC
CTACGCACAGGCCATTGCCCATGGTGACGCCGCCATCAACCAGTGCCCCCCGGGCGGCGCGGAGGGTATCCAGCGGCTGG
CAGACATCACCGGCTTGCCCGTGCTGCCACTGAACCCGGCCAACGGCACCGAAGGCCCGCGCGGGGTGGCCATCATCGAC
GAAGCCTGGTGCATAGGCTGCACGCTGTGCATCAAGGCCTGCCCGACCGATGCCATTGTGGGCAGCAACAAGATGATGCA
CACCGTGATTGAGCCTTACTGCACAGGCTGCGAGCTCTGCATCCCCGTGTGTCCGGTTGACTGCATCAGCCTCGAAAATG
TCTCGGGCACGCGCACCGGCTGGAGTGCCTGGTCTCAGGAAGCTGCCGACACAGCCCTCCATCGCTATACTTTTCATAGC
ATTCGACTGGAAAAAGATGGGACTCGGCAAGCTAAAAAACCTGCAGACCCGGAGAAGAAAGCAGACCTCCCGGTGATAGG
CCGGCCTGCCCGCTCCGAATCAGACACAGCCATCGTGGAGCGAAAGCGCGCGGTGATTGAAGCGGCGCTGGCCCGCGCCC
GGGCACAACGCCAACACAAGCCCTGA

Upstream 100 bases:

>100_bases
GTGATTTCCCTTCCGGCGCAGCAGTAGCCCGACATTCCTGAACCAATTCGCGCGCCGGCCTCAACGACGGCTTATCATCA
CCGGCCGCTGCAAACTCCCC

Downstream 100 bases:

>100_bases
CAGAAAGACCGGCTACCCGGGGCTCGCCCCGGGCTCCGTCCTGCAAACGGTCAGGCCGGCAGCTCGGTCAGGCCCAGCAC
CTCGGCTTCGTAGGCATGCA

Product: RnfABCDGE type electron transport complex subunit B

Products: NA

Alternate protein names: Nitrogen fixation protein rnfB [H]

Number of amino acids: Translated: 221; Mature: 221

Protein sequence:

>221_residues
MNDLTARLHAALPQTQCTRCGYPDCAAYAQAIAHGDAAINQCPPGGAEGIQRLADITGLPVLPLNPANGTEGPRGVAIID
EAWCIGCTLCIKACPTDAIVGSNKMMHTVIEPYCTGCELCIPVCPVDCISLENVSGTRTGWSAWSQEAADTALHRYTFHS
IRLEKDGTRQAKKPADPEKKADLPVIGRPARSESDTAIVERKRAVIEAALARARAQRQHKP

Sequences:

>Translated_221_residues
MNDLTARLHAALPQTQCTRCGYPDCAAYAQAIAHGDAAINQCPPGGAEGIQRLADITGLPVLPLNPANGTEGPRGVAIID
EAWCIGCTLCIKACPTDAIVGSNKMMHTVIEPYCTGCELCIPVCPVDCISLENVSGTRTGWSAWSQEAADTALHRYTFHS
IRLEKDGTRQAKKPADPEKKADLPVIGRPARSESDTAIVERKRAVIEAALARARAQRQHKP
>Mature_221_residues
MNDLTARLHAALPQTQCTRCGYPDCAAYAQAIAHGDAAINQCPPGGAEGIQRLADITGLPVLPLNPANGTEGPRGVAIID
EAWCIGCTLCIKACPTDAIVGSNKMMHTVIEPYCTGCELCIPVCPVDCISLENVSGTRTGWSAWSQEAADTALHRYTFHS
IRLEKDGTRQAKKPADPEKKADLPVIGRPARSESDTAIVERKRAVIEAALARARAQRQHKP

Specific function: Required for nitrogen fixation. May be part of a membrane complex functioning as an intermediate in the electron transport to nitrogenase [H]

COG id: COG2878

COG function: function code C; Predicted NADH:ubiquinone oxidoreductase, subunit RnfB

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787915, Length=139, Percent_Identity=46.0431654676259, Blast_Score=125, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR010207
- InterPro:   IPR016463
- InterPro:   IPR007202
- InterPro:   IPR012285 [H]

Pfam domain/function: PF00037 Fer4; PF04060 FeS [H]

EC number: NA

Molecular weight: Translated: 23636; Mature: 23636

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
7.2 %Cys+Met (Translated Protein)
5.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
7.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDLTARLHAALPQTQCTRCGYPDCAAYAQAIAHGDAAINQCPPGGAEGIQRLADITGLP
CCCHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHCCCC
VLPLNPANGTEGPRGVAIIDEAWCIGCTLCIKACPTDAIVGSNKMMHTVIEPYCTGCELC
EEECCCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHCCCCEE
IPVCPVDCISLENVSGTRTGWSAWSQEAADTALHRYTFHSIRLEKDGTRQAKKPADPEKK
ECCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCHH
ADLPVIGRPARSESDTAIVERKRAVIEAALARARAQRQHKP
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNDLTARLHAALPQTQCTRCGYPDCAAYAQAIAHGDAAINQCPPGGAEGIQRLADITGLP
CCCHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHCCCC
VLPLNPANGTEGPRGVAIIDEAWCIGCTLCIKACPTDAIVGSNKMMHTVIEPYCTGCELC
EEECCCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHCCCCEE
IPVCPVDCISLENVSGTRTGWSAWSQEAADTALHRYTFHSIRLEKDGTRQAKKPADPEKK
ECCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCHH
ADLPVIGRPARSESDTAIVERKRAVIEAALARARAQRQHKP
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA