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The map label for this gene is gsiB [H]

Identifier: 91787986

GI number: 91787986

Start: 2203070

End: 2204653

Strand: Direct

Name: gsiB [H]

Synonym: Bpro_2113

Alternate gene names: 91787986

Gene position: 2203070-2204653 (Clockwise)

Preceding gene: 91787985

Following gene: 91787987

Centisome position: 42.36

GC content: 60.8

Gene sequence:

>1584_bases
ATGAAATTCAAGCCAAACCTGCTCTCGGTCGCCGTGTTTTGTGCGGTAGCTGCTACAAGTTTTGCAGCGTCAGCGCAGAC
CCTGCGCGTCGCCAACCAGGGTGACGCGCTGTCCATGGACCCGCATTCGCTCAACGAATCGATGCAGCTCAGTGTGACGG
GCAATGTTTACGAAGGCCTGGTGATTCGCAACAAGGACCTGAGCCTGGCCCCGGGCCTGGCGACCGCCTGGAAGCAGACC
TCGCCGACCGTGTGGCGTTTTGAGCTGCGCAAGGGCGTGCAGTTTCATGACGGCACGCCTTTCACGGCCGATGACGTGGT
GTTCAGCTTCCAGCGCACGCAGGTCGAAGGCTCCGACATGAAGAGCTACACCAACGACTTCAAGGAAGTGCGTAAGGTCA
ACGACCACGTGGTCGAAATCGAAACCAAGACGCCTTTTCCCATCCTTCCTGATGTGATCTCGCTGGTCTACATGATGAGC
AAGAAGTGGTGCGAGACCAATCAGGCAGCCAAGCCGGTGGACCGTCGCAAGGGTGTCGAGAACGCCGCCTCGTTCCGCGC
CAACGGCACGGGCCCGTTCCGTGTGCGCGAGCGCCAGCCCAATGTGCGAACCACCTTTGTCCGCAACGGCAACTACTGGG
GCAAGATCGAGGGCAACGTTCAGGAGGTGGTCTTCACCCCGATCGGCAATGACGCGACCCGCGTGGCTGCCCTGCTGTCG
GGCGAGATCGACGTCATGGAGCCGGTGCCGGTTCAGGATATCGACCGCGTCAACAGTGCCCCTGCCACCCGCGTGCTGGC
CGGCCCCGAGTTGCGCACCATCTTTTTGGGCATGGACCAGAAGCGCGACGAGCTGCTGTATTCCAGCGTCAAGGGCAAGA
ATCCGTTCAAGGACAAGCGCGTGCGCCAGGCCTTCTACCAGGCCATCGATATCGAGGGCATCAAGAAGACTGTGATGCGT
GGCGGCTCGAACACGACCGCGCTGCTGGTGGGCCCCGGCATCAATGGCTTCCAGGCCGATCAGAACAAGCGACTGCCGTA
TGACCCGGAAGCAGCCAAGAAGCTGATGGCGGAGGCCGGTTACCCGAACGGCTTCGAGGTCTCCATGAACTGCCCGAACG
ACCGCTATGTGAATGACAGCCGGATTTGCCAGACCGTGGCCGCCAACCTGTCGCGCATCAACGTTAAGATCAACCTGCAG
GCTGAGACCAAGGGCAGCTACTTCCCTAAGGTGCTGCGCCGCGACACCAGCTTCTACATGCTGGGCTGGACGCCCAGCAC
CTACGACGCGCACAACGCGCTCAACGCGCTGCTCGCCTGCGTCGACGACAAAGGTGCCGGCCAATTCAACCTGGGGGCTT
ATTGCAATCCCAAGGTCGACGAGCTCACCAAGAAGGTGCAGGCCGAGACCGACAAGGCCAAGCGCAACGCCATGATCAAG
GAAGCGTTCGAGATCCATTCGGCCGACATCGGCCACCTGCCGCTGCACCAGCAGGCGCTGGCCTGGGGCATGAGCAAGAA
GGTCGAGTTGGTCCAGCTGGCCGACAACTTCATGTTCTACAAGTGGGTGAGCGTCAAAAAATAA

Upstream 100 bases:

>100_bases
TGGCCATGCTGGGGGCCACTGATGCTGGCCTGCCACTGAATCATTGAGCCGACAATTTTTACCCACCCCAAGCCCTCATC
TCTTCCATAAGGAGTTCCCG

Downstream 100 bases:

>100_bases
GGGCGTAATGTCTTGGGCGCCGGCGGATCACACCCGCCAGCGCCCTCTTGGCTTTCGTACCTCTCTATCTCGCACCTCAC
GATGAAAAAAATCTTTGCCC

Product: extracellular solute-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 527; Mature: 527

Protein sequence:

>527_residues
MKFKPNLLSVAVFCAVAATSFAASAQTLRVANQGDALSMDPHSLNESMQLSVTGNVYEGLVIRNKDLSLAPGLATAWKQT
SPTVWRFELRKGVQFHDGTPFTADDVVFSFQRTQVEGSDMKSYTNDFKEVRKVNDHVVEIETKTPFPILPDVISLVYMMS
KKWCETNQAAKPVDRRKGVENAASFRANGTGPFRVRERQPNVRTTFVRNGNYWGKIEGNVQEVVFTPIGNDATRVAALLS
GEIDVMEPVPVQDIDRVNSAPATRVLAGPELRTIFLGMDQKRDELLYSSVKGKNPFKDKRVRQAFYQAIDIEGIKKTVMR
GGSNTTALLVGPGINGFQADQNKRLPYDPEAAKKLMAEAGYPNGFEVSMNCPNDRYVNDSRICQTVAANLSRINVKINLQ
AETKGSYFPKVLRRDTSFYMLGWTPSTYDAHNALNALLACVDDKGAGQFNLGAYCNPKVDELTKKVQAETDKAKRNAMIK
EAFEIHSADIGHLPLHQQALAWGMSKKVELVQLADNFMFYKWVSVKK

Sequences:

>Translated_527_residues
MKFKPNLLSVAVFCAVAATSFAASAQTLRVANQGDALSMDPHSLNESMQLSVTGNVYEGLVIRNKDLSLAPGLATAWKQT
SPTVWRFELRKGVQFHDGTPFTADDVVFSFQRTQVEGSDMKSYTNDFKEVRKVNDHVVEIETKTPFPILPDVISLVYMMS
KKWCETNQAAKPVDRRKGVENAASFRANGTGPFRVRERQPNVRTTFVRNGNYWGKIEGNVQEVVFTPIGNDATRVAALLS
GEIDVMEPVPVQDIDRVNSAPATRVLAGPELRTIFLGMDQKRDELLYSSVKGKNPFKDKRVRQAFYQAIDIEGIKKTVMR
GGSNTTALLVGPGINGFQADQNKRLPYDPEAAKKLMAEAGYPNGFEVSMNCPNDRYVNDSRICQTVAANLSRINVKINLQ
AETKGSYFPKVLRRDTSFYMLGWTPSTYDAHNALNALLACVDDKGAGQFNLGAYCNPKVDELTKKVQAETDKAKRNAMIK
EAFEIHSADIGHLPLHQQALAWGMSKKVELVQLADNFMFYKWVSVKK
>Mature_527_residues
MKFKPNLLSVAVFCAVAATSFAASAQTLRVANQGDALSMDPHSLNESMQLSVTGNVYEGLVIRNKDLSLAPGLATAWKQT
SPTVWRFELRKGVQFHDGTPFTADDVVFSFQRTQVEGSDMKSYTNDFKEVRKVNDHVVEIETKTPFPILPDVISLVYMMS
KKWCETNQAAKPVDRRKGVENAASFRANGTGPFRVRERQPNVRTTFVRNGNYWGKIEGNVQEVVFTPIGNDATRVAALLS
GEIDVMEPVPVQDIDRVNSAPATRVLAGPELRTIFLGMDQKRDELLYSSVKGKNPFKDKRVRQAFYQAIDIEGIKKTVMR
GGSNTTALLVGPGINGFQADQNKRLPYDPEAAKKLMAEAGYPNGFEVSMNCPNDRYVNDSRICQTVAANLSRINVKINLQ
AETKGSYFPKVLRRDTSFYMLGWTPSTYDAHNALNALLACVDDKGAGQFNLGAYCNPKVDELTKKVQAETDKAKRNAMIK
EAFEIHSADIGHLPLHQQALAWGMSKKVELVQLADNFMFYKWVSVKK

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=514, Percent_Identity=25.2918287937743, Blast_Score=146, Evalue=3e-36,
Organism=Escherichia coli, GI1787052, Length=516, Percent_Identity=24.4186046511628, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1787495, Length=522, Percent_Identity=24.7126436781609, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI87081878, Length=521, Percent_Identity=23.0326295585413, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1787762, Length=521, Percent_Identity=22.4568138195777, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1789887, Length=518, Percent_Identity=23.5521235521236, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1789397, Length=522, Percent_Identity=23.5632183908046, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI1787551, Length=454, Percent_Identity=23.1277533039648, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 58656; Mature: 58656

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFKPNLLSVAVFCAVAATSFAASAQTLRVANQGDALSMDPHSLNESMQLSVTGNVYEGL
CCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEECCHHHCCCCEEEEEECCCEECE
VIRNKDLSLAPGLATAWKQTSPTVWRFELRKGVQFHDGTPFTADDVVFSFQRTQVEGSDM
EEECCCCCCCCCHHHHHCCCCCCEEEEEECCCCEECCCCCCCHHHHEEEEHHCCCCCCHH
KSYTNDFKEVRKVNDHVVEIETKTPFPILPDVISLVYMMSKKWCETNQAAKPVDRRKGVE
HHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCC
NAASFRANGTGPFRVRERQPNVRTTFVRNGNYWGKIEGNVQEVVFTPIGNDATRVAALLS
HHHHCCCCCCCCEEEECCCCCCEEEEEECCCEEEEECCCEEEEEEEECCCCHHHHHHHHC
GEIDVMEPVPVQDIDRVNSAPATRVLAGPELRTIFLGMDQKRDELLYSSVKGKNPFKDKR
CCCEEECCCCHHHHHHCCCCCCCEEECCCCEEEEEECCCCCHHHHHHHHCCCCCCCHHHH
VRQAFYQAIDIEGIKKTVMRGGSNTTALLVGPGINGFQADQNKRLPYDPEAAKKLMAEAG
HHHHHHHHHCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCC
YPNGFEVSMNCPNDRYVNDSRICQTVAANLSRINVKINLQAETKGSYFPKVLRRDTSFYM
CCCCEEEEEECCCCCCCCHHHHHHHHHCCCEEEEEEEEEEECCCCCHHHHHHHCCCCEEE
LGWTPSTYDAHNALNALLACVDDKGAGQFNLGAYCNPKVDELTKKVQAETDKAKRNAMIK
EECCCCCCCHHHHHHHHHHHCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
EAFEIHSADIGHLPLHQQALAWGMSKKVELVQLADNFMFYKWVSVKK
HHHHHHCCCCCCCCCHHHHHHHCCCCCCHHEEHHCCEEEEEEEEECC
>Mature Secondary Structure
MKFKPNLLSVAVFCAVAATSFAASAQTLRVANQGDALSMDPHSLNESMQLSVTGNVYEGL
CCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEECCHHHCCCCEEEEEECCCEECE
VIRNKDLSLAPGLATAWKQTSPTVWRFELRKGVQFHDGTPFTADDVVFSFQRTQVEGSDM
EEECCCCCCCCCHHHHHCCCCCCEEEEEECCCCEECCCCCCCHHHHEEEEHHCCCCCCHH
KSYTNDFKEVRKVNDHVVEIETKTPFPILPDVISLVYMMSKKWCETNQAAKPVDRRKGVE
HHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCC
NAASFRANGTGPFRVRERQPNVRTTFVRNGNYWGKIEGNVQEVVFTPIGNDATRVAALLS
HHHHCCCCCCCCEEEECCCCCCEEEEEECCCEEEEECCCEEEEEEEECCCCHHHHHHHHC
GEIDVMEPVPVQDIDRVNSAPATRVLAGPELRTIFLGMDQKRDELLYSSVKGKNPFKDKR
CCCEEECCCCHHHHHHCCCCCCCEEECCCCEEEEEECCCCCHHHHHHHHCCCCCCCHHHH
VRQAFYQAIDIEGIKKTVMRGGSNTTALLVGPGINGFQADQNKRLPYDPEAAKKLMAEAG
HHHHHHHHHCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCC
YPNGFEVSMNCPNDRYVNDSRICQTVAANLSRINVKINLQAETKGSYFPKVLRRDTSFYM
CCCCEEEEEECCCCCCCCHHHHHHHHHCCCEEEEEEEEEEECCCCCHHHHHHHCCCCEEE
LGWTPSTYDAHNALNALLACVDDKGAGQFNLGAYCNPKVDELTKKVQAETDKAKRNAMIK
EECCCCCCCHHHHHHHHHHHCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
EAFEIHSADIGHLPLHQQALAWGMSKKVELVQLADNFMFYKWVSVKK
HHHHHHCCCCCCCCCHHHHHHHCCCCCCHHEEHHCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA