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The map label for this gene is potI [H]

Identifier: 91787971

GI number: 91787971

Start: 2186422

End: 2187300

Strand: Direct

Name: potI [H]

Synonym: Bpro_2098

Alternate gene names: 91787971

Gene position: 2186422-2187300 (Clockwise)

Preceding gene: 91787970

Following gene: 91787973

Centisome position: 42.04

GC content: 60.86

Gene sequence:

>879_bases
ATGAGAAGCCTGTGGCGCTTTGCAGAAAACCAGGTGGGCCGGCTCTGGCTGGGCGCGGTCTATCTGTTCCTCTACATCCC
GCTGTTCTTCCTCATCGTCTTTTCTTTCAACAGCACCCGTCAGGATGGCGTGTTCACCGGCTTTTCCTTGCGCTGGTATG
AGGCCCTGCTGGCAGACTCCCGTCTGGTCGAGGGATTTTTCCTGTCGCTCAAGGTGGCGCTGATTACCGGCACGCTGTCG
GTGGTACTGGGCAGCTTTGCCGCGTTTGTATTGGTGCGCTACCTCCGCTTCACGGGGCGCACGCTGTTTTACGGCATGGT
GAATGCGCCGCTGGTGATGCCCGAAGTCATTATTGGGCTGTCTTTGTTGCTGCTGATGGTGGCTGTGCAGCGCGCGGTGG
GTTGGCCCGACCGGGGCTTCATCACCATCGTGACCGGCCACACGCTGCTCGGCATGGCTTATGCCACGGTCGTGATCCAG
TCGCGCCTGAGCGAGATGGACCGCTCGCTGGAGGAGGCCGCCATGGACCTGGGATGCCGGCCCATGCAGGTTTTTTTCCT
GGTGACCCTGCCCAATATTGCACCGGCCCTGGTCTCGGCCTGGCTGCTGACCTTTACCCTGTCATTTGACGACGTGGTGA
TCTCGGAGTTTTTGTCAGGCCCGGGCGTGACGACCTTGCCCCAGGTGATTTTCAGCTACGCGCGCCGTGGCGTGAACCCG
TCCATCTATGCGGCTGCGACTATCCTGATTGCCGTGGTTTCGCTGGGCATCATCACGTACAGCATCTATGTGATGCGCCG
GCAGAAACGGCTGGACCGTGAAATCAGTGCGGCGGCTCGACTTGAACAGAGCTTCCCCGACCCCGCCCGGGCAGGGTAG

Upstream 100 bases:

>100_bases
GCGGCGGCCCTGGCCGTCGTGATGATTCTGCTCATCCTGGTGCCTTTGGCCTTTTACTACCGTGCAAGCGCCGCGCGCGA
TGCTCACGGAGAATCGTGAC

Downstream 100 bases:

>100_bases
CCAGCACCACGCCGGCCAATGCAATGGCGAATGCGCCCATCTGGGTGCCGTTCAGTGTTTCGCCCAGCACGATGACACCC
ACTGCTGCGGCGCTGACGGG

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MRSLWRFAENQVGRLWLGAVYLFLYIPLFFLIVFSFNSTRQDGVFTGFSLRWYEALLADSRLVEGFFLSLKVALITGTLS
VVLGSFAAFVLVRYLRFTGRTLFYGMVNAPLVMPEVIIGLSLLLLMVAVQRAVGWPDRGFITIVTGHTLLGMAYATVVIQ
SRLSEMDRSLEEAAMDLGCRPMQVFFLVTLPNIAPALVSAWLLTFTLSFDDVVISEFLSGPGVTTLPQVIFSYARRGVNP
SIYAAATILIAVVSLGIITYSIYVMRRQKRLDREISAAARLEQSFPDPARAG

Sequences:

>Translated_292_residues
MRSLWRFAENQVGRLWLGAVYLFLYIPLFFLIVFSFNSTRQDGVFTGFSLRWYEALLADSRLVEGFFLSLKVALITGTLS
VVLGSFAAFVLVRYLRFTGRTLFYGMVNAPLVMPEVIIGLSLLLLMVAVQRAVGWPDRGFITIVTGHTLLGMAYATVVIQ
SRLSEMDRSLEEAAMDLGCRPMQVFFLVTLPNIAPALVSAWLLTFTLSFDDVVISEFLSGPGVTTLPQVIFSYARRGVNP
SIYAAATILIAVVSLGIITYSIYVMRRQKRLDREISAAARLEQSFPDPARAG
>Mature_292_residues
MRSLWRFAENQVGRLWLGAVYLFLYIPLFFLIVFSFNSTRQDGVFTGFSLRWYEALLADSRLVEGFFLSLKVALITGTLS
VVLGSFAAFVLVRYLRFTGRTLFYGMVNAPLVMPEVIIGLSLLLLMVAVQRAVGWPDRGFITIVTGHTLLGMAYATVVIQ
SRLSEMDRSLEEAAMDLGCRPMQVFFLVTLPNIAPALVSAWLLTFTLSFDDVVISEFLSGPGVTTLPQVIFSYARRGVNP
SIYAAATILIAVVSLGIITYSIYVMRRQKRLDREISAAARLEQSFPDPARAG

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1177

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component II

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787081, Length=283, Percent_Identity=49.8233215547703, Blast_Score=230, Evalue=8e-62,
Organism=Escherichia coli, GI1787368, Length=265, Percent_Identity=35.4716981132075, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1787714, Length=209, Percent_Identity=32.5358851674641, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1788764, Length=177, Percent_Identity=29.9435028248588, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32479; Mature: 32479

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSLWRFAENQVGRLWLGAVYLFLYIPLFFLIVFSFNSTRQDGVFTGFSLRWYEALLADS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCHHHHHHHHHHHH
RLVEGFFLSLKVALITGTLSVVLGSFAAFVLVRYLRFTGRTLFYGMVNAPLVMPEVIIGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHH
SLLLLMVAVQRAVGWPDRGFITIVTGHTLLGMAYATVVIQSRLSEMDRSLEEAAMDLGCR
HHHHHHHHHHHHCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PMQVFFLVTLPNIAPALVSAWLLTFTLSFDDVVISEFLSGPGVTTLPQVIFSYARRGVNP
HHHHHEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCC
SIYAAATILIAVVSLGIITYSIYVMRRQKRLDREISAAARLEQSFPDPARAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MRSLWRFAENQVGRLWLGAVYLFLYIPLFFLIVFSFNSTRQDGVFTGFSLRWYEALLADS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCHHHHHHHHHHHH
RLVEGFFLSLKVALITGTLSVVLGSFAAFVLVRYLRFTGRTLFYGMVNAPLVMPEVIIGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHH
SLLLLMVAVQRAVGWPDRGFITIVTGHTLLGMAYATVVIQSRLSEMDRSLEEAAMDLGCR
HHHHHHHHHHHHCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PMQVFFLVTLPNIAPALVSAWLLTFTLSFDDVVISEFLSGPGVTTLPQVIFSYARRGVNP
HHHHHEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCC
SIYAAATILIAVVSLGIITYSIYVMRRQKRLDREISAAARLEQSFPDPARAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]