Click here to switch to the map view.

The map label for this gene is potH [H]

Identifier: 91787970

GI number: 91787970

Start: 2185479

End: 2186420

Strand: Direct

Name: potH [H]

Synonym: Bpro_2097

Alternate gene names: 91787970

Gene position: 2185479-2186420 (Clockwise)

Preceding gene: 91787969

Following gene: 91787971

Centisome position: 42.03

GC content: 59.55

Gene sequence:

>942_bases
ATGAAAATCCCCTCCTGGCTCCAGTTCAACCCCGGGCGGCGCTTTGTCATCGGCGTGCCCTACGTCTGGCTGCTGCTGTT
TTTTGTGCTGCCTTTCCTGATCATGCTGCGCATCAGCGTGACGGACATGGGCAATGGCGTGGATCCTTTTGCGCCGCTGG
TGGAGGCCGTGTCGGGCTCCTGGCGTGTCATGCTGCGTTATGCAAATTACGTTTCCATCTTTCACGACGCAGGGCAGGGG
GCCGATAGCGGATGGGGTAACACCATCTACATGGAGGGCTACGAGACCTCCCTGAAATACGCCTTGTTCACCACAGTGTC
GTGCCTGGTGATTGGCTATCCGTTTGCCTATTTCATTGCCCGGTCGCGGCCCTCCATTCGCCCGGCCTTGCTGATGCTGG
TCATGCTGCCTTTCTGGACCTCGTTCCTGCTGCGCGTCTATGCGTGGAAGGGGATCCTGGCCGACCAGGGCGTGCTCAAC
AACCTGCTGCTGGCGCTGGGCATCATCGATGAACCCATGGTGATGCTCTACACCAATGTGTCCATGCTGGTCGGCATGAC
CTATGTGTATTGCCCCTTCATGATCCTGCCGCTCTATGCCAACCTGGTGAAGATGGATTTCCGCCTGCTGGAAGCGGCCC
ATGACCTGGGGGCCAGCGCCTGGAAGGCGTTCTGGCTGATCACCGTGCCGTTGAGCAAATCCGGCATCATTGCCGGCTCC
ATGCTGGTCTTCATTCCCAGTGTGGGTGAGTTCGTCATTCCCTCGCTGCTGGGCGGCGCCGAAAACATCATGATCGGCCG
CGTGGTCTGGGACGAAATGTTCAGCAGCAACAACTGGCCGCGCGCGGCGGCCCTGGCCGTCGTGATGATTCTGCTCATCC
TGGTGCCTTTGGCCTTTTACTACCGTGCAAGCGCCGCGCGCGATGCTCACGGAGAATCGTGA

Upstream 100 bases:

>100_bases
ACCACGCGGCATGAAGGCAGCGTGATCACCTGGGATGACCCGGTCTTTTTCTGGTGGGATGACTCGGCACCGGTGGTGCT
GACCCAATAAAGCCGCCGTG

Downstream 100 bases:

>100_bases
CATGAGAAGCCTGTGGCGCTTTGCAGAAAACCAGGTGGGCCGGCTCTGGCTGGGCGCGGTCTATCTGTTCCTCTACATCC
CGCTGTTCTTCCTCATCGTC

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MKIPSWLQFNPGRRFVIGVPYVWLLLFFVLPFLIMLRISVTDMGNGVDPFAPLVEAVSGSWRVMLRYANYVSIFHDAGQG
ADSGWGNTIYMEGYETSLKYALFTTVSCLVIGYPFAYFIARSRPSIRPALLMLVMLPFWTSFLLRVYAWKGILADQGVLN
NLLLALGIIDEPMVMLYTNVSMLVGMTYVYCPFMILPLYANLVKMDFRLLEAAHDLGASAWKAFWLITVPLSKSGIIAGS
MLVFIPSVGEFVIPSLLGGAENIMIGRVVWDEMFSSNNWPRAAALAVVMILLILVPLAFYYRASAARDAHGES

Sequences:

>Translated_313_residues
MKIPSWLQFNPGRRFVIGVPYVWLLLFFVLPFLIMLRISVTDMGNGVDPFAPLVEAVSGSWRVMLRYANYVSIFHDAGQG
ADSGWGNTIYMEGYETSLKYALFTTVSCLVIGYPFAYFIARSRPSIRPALLMLVMLPFWTSFLLRVYAWKGILADQGVLN
NLLLALGIIDEPMVMLYTNVSMLVGMTYVYCPFMILPLYANLVKMDFRLLEAAHDLGASAWKAFWLITVPLSKSGIIAGS
MLVFIPSVGEFVIPSLLGGAENIMIGRVVWDEMFSSNNWPRAAALAVVMILLILVPLAFYYRASAARDAHGES
>Mature_313_residues
MKIPSWLQFNPGRRFVIGVPYVWLLLFFVLPFLIMLRISVTDMGNGVDPFAPLVEAVSGSWRVMLRYANYVSIFHDAGQG
ADSGWGNTIYMEGYETSLKYALFTTVSCLVIGYPFAYFIARSRPSIRPALLMLVMLPFWTSFLLRVYAWKGILADQGVLN
NLLLALGIIDEPMVMLYTNVSMLVGMTYVYCPFMILPLYANLVKMDFRLLEAAHDLGASAWKAFWLITVPLSKSGIIAGS
MLVFIPSVGEFVIPSLLGGAENIMIGRVVWDEMFSSNNWPRAAALAVVMILLILVPLAFYYRASAARDAHGES

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=308, Percent_Identity=48.3766233766234, Blast_Score=285, Evalue=4e-78,
Organism=Escherichia coli, GI1787369, Length=226, Percent_Identity=41.5929203539823, Blast_Score=190, Evalue=1e-49,
Organism=Escherichia coli, GI1787713, Length=223, Percent_Identity=33.1838565022422, Blast_Score=113, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 34945; Mature: 34945

Theoretical pI: Translated: 8.15; Mature: 8.15

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIPSWLQFNPGRRFVIGVPYVWLLLFFVLPFLIMLRISVTDMGNGVDPFAPLVEAVSGS
CCCCCCCEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCC
WRVMLRYANYVSIFHDAGQGADSGWGNTIYMEGYETSLKYALFTTVSCLVIGYPFAYFIA
HHEEHHHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RSRPSIRPALLMLVMLPFWTSFLLRVYAWKGILADQGVLNNLLLALGIIDEPMVMLYTNV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCHHHHHHHH
SMLVGMTYVYCPFMILPLYANLVKMDFRLLEAAHDLGASAWKAFWLITVPLSKSGIIAGS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHEEEEEEEECCCCCCCHHHH
MLVFIPSVGEFVIPSLLGGAENIMIGRVVWDEMFSSNNWPRAAALAVVMILLILVPLAFY
HHHEECCCHHHHHHHHHCCCCCEEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
YRASAARDAHGES
HHHHHCCCCCCCC
>Mature Secondary Structure
MKIPSWLQFNPGRRFVIGVPYVWLLLFFVLPFLIMLRISVTDMGNGVDPFAPLVEAVSGS
CCCCCCCEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCC
WRVMLRYANYVSIFHDAGQGADSGWGNTIYMEGYETSLKYALFTTVSCLVIGYPFAYFIA
HHEEHHHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RSRPSIRPALLMLVMLPFWTSFLLRVYAWKGILADQGVLNNLLLALGIIDEPMVMLYTNV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCHHHHHHHH
SMLVGMTYVYCPFMILPLYANLVKMDFRLLEAAHDLGASAWKAFWLITVPLSKSGIIAGS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHEEEEEEEECCCCCCCHHHH
MLVFIPSVGEFVIPSLLGGAENIMIGRVVWDEMFSSNNWPRAAALAVVMILLILVPLAFY
HHHEECCCHHHHHHHHHCCCCCEEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
YRASAARDAHGES
HHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]