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The map label for this gene is potF [H]

Identifier: 91787968

GI number: 91787968

Start: 2183094

End: 2184251

Strand: Direct

Name: potF [H]

Synonym: Bpro_2095

Alternate gene names: 91787968

Gene position: 2183094-2184251 (Clockwise)

Preceding gene: 91787967

Following gene: 91787969

Centisome position: 41.98

GC content: 59.15

Gene sequence:

>1158_bases
ATGATAAGAAAGCATTTGTGGATTTTTGCCTTTTCTGCGGTGGCACTGAGTGCCTGCGGCAAAAAGGAGGAGGCACCTGC
GGTTGCAACGCCGGCCGCCCCGGCCAGCGCGGCTGTGGCGGTGAACACCGAAGAGAAGGTGCTCAACGTCTACAACTGGC
CGGACTACGTCGCCAAGGACATGGTGGCTAACTTCGAGAAGGAAACGGGCATCAAGGTCAATTACCAGACCTTTGAGAAC
AACGAAGCCCTGCACGCCAAGCTGGTGGCCGGCAATACCGGCTACGACATCGTCGTGCCCGGCGCCGTGTTTGCCAAGCC
GCAAATTGATGGCGGGCTGCTGATGAAGCTGGACAAGTCCAAGATCAGCAACTATGGCAACCTCGACTCGCCCATCATGG
AAAAGTTGGCCACCATTGACCCGGGCAACGCCTACCTGGTGCCCTGGGCCTGGAGTTTCACCACGGTGGGCATCAACAAG
GCCAAGGTCGCCAAGGCGTTGGGCTCAACACCCATGCCTGAAAACGCCTGGGAGCTGGTGTTCAACCCGGTCTATACGGC
CAAACTGAAGTCCTGCGGTATCGCCTTCCTGGATTCGCCGACCGAGGTGATTCCACCGGCCATGCACTACCTGGGCAAGA
ACGCCTATTCGAACGATCCGGCCGACCACAAGGCCGCGGCCGCCATGCTGGCCAAGGTACGCCCGCACATCCGCATGTTC
TCCAGCACCATGATTGATGACCTGGCCGGTGGCAAGGCTTGCGTGGCGCTGGCTTGGGCCGGGGACATCAACATTGCCCG
TGGCCGCGCGATTGAAAACAAGAGTGGCAACGACGTCCAGGCGCTGTTGCCCAGCACCGGTGGCCTGATTTTCTTTGACA
CCCTGGCTATTCCCAAAGATGCCAAGCACCCGAACAACGCTTTGGCGTTCATCAACTACTCCCTGCGCCCCGAAGTGTCT
GCCTCGCTGACCAATGAACTGGGCTACGCCACGGCCAACAAGGCCAGCCTGGCCAGCGTCAAGCCGGAAATCGCCCAGGA
CAAAGCCGTGTTCCCCGATGCAGCCAACCTGCAGAAAATGGTCTCTCCCGCCAGCTTCAGCAACGAAGCGCGGGAATCCA
TGAGCAACGTCTTTACCCTGTTCAAAAAAGGCAGTTGA

Upstream 100 bases:

>100_bases
CTCTTATACAATCTGCGGCTGGCTGGCCTTTGCGTCCTGCAGTGCCACCTGCCGCGTGTGTTTGCCCTTTTGTTGTTACC
TTTCCTGACGGAGCCCGGAC

Downstream 100 bases:

>100_bases
GTTTTCGGGCCATGCCCCGTGGGCTGGCTGATCAGGCTGTGCACGCGATCCAGCGTGCACAGCCTTTTTGATGTGGTTTT
GCTACCTGGGAACTACGCCT

Product: extracellular solute-binding protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MIRKHLWIFAFSAVALSACGKKEEAPAVATPAAPASAAVAVNTEEKVLNVYNWPDYVAKDMVANFEKETGIKVNYQTFEN
NEALHAKLVAGNTGYDIVVPGAVFAKPQIDGGLLMKLDKSKISNYGNLDSPIMEKLATIDPGNAYLVPWAWSFTTVGINK
AKVAKALGSTPMPENAWELVFNPVYTAKLKSCGIAFLDSPTEVIPPAMHYLGKNAYSNDPADHKAAAAMLAKVRPHIRMF
SSTMIDDLAGGKACVALAWAGDINIARGRAIENKSGNDVQALLPSTGGLIFFDTLAIPKDAKHPNNALAFINYSLRPEVS
ASLTNELGYATANKASLASVKPEIAQDKAVFPDAANLQKMVSPASFSNEARESMSNVFTLFKKGS

Sequences:

>Translated_385_residues
MIRKHLWIFAFSAVALSACGKKEEAPAVATPAAPASAAVAVNTEEKVLNVYNWPDYVAKDMVANFEKETGIKVNYQTFEN
NEALHAKLVAGNTGYDIVVPGAVFAKPQIDGGLLMKLDKSKISNYGNLDSPIMEKLATIDPGNAYLVPWAWSFTTVGINK
AKVAKALGSTPMPENAWELVFNPVYTAKLKSCGIAFLDSPTEVIPPAMHYLGKNAYSNDPADHKAAAAMLAKVRPHIRMF
SSTMIDDLAGGKACVALAWAGDINIARGRAIENKSGNDVQALLPSTGGLIFFDTLAIPKDAKHPNNALAFINYSLRPEVS
ASLTNELGYATANKASLASVKPEIAQDKAVFPDAANLQKMVSPASFSNEARESMSNVFTLFKKGS
>Mature_385_residues
MIRKHLWIFAFSAVALSACGKKEEAPAVATPAAPASAAVAVNTEEKVLNVYNWPDYVAKDMVANFEKETGIKVNYQTFEN
NEALHAKLVAGNTGYDIVVPGAVFAKPQIDGGLLMKLDKSKISNYGNLDSPIMEKLATIDPGNAYLVPWAWSFTTVGINK
AKVAKALGSTPMPENAWELVFNPVYTAKLKSCGIAFLDSPTEVIPPAMHYLGKNAYSNDPADHKAAAAMLAKVRPHIRMF
SSTMIDDLAGGKACVALAWAGDINIARGRAIENKSGNDVQALLPSTGGLIFFDTLAIPKDAKHPNNALAFINYSLRPEVS
ASLTNELGYATANKASLASVKPEIAQDKAVFPDAANLQKMVSPASFSNEARESMSNVFTLFKKGS

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=335, Percent_Identity=44.4776119402985, Blast_Score=283, Evalue=9e-78,
Organism=Escherichia coli, GI1787367, Length=317, Percent_Identity=33.4384858044164, Blast_Score=182, Evalue=3e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 41212; Mature: 41212

Theoretical pI: Translated: 8.41; Mature: 8.41

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRKHLWIFAFSAVALSACGKKEEAPAVATPAAPASAAVAVNTEEKVLNVYNWPDYVAKD
CCCHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCEEEEECCHHHHEEECCCHHHHHHH
MVANFEKETGIKVNYQTFENNEALHAKLVAGNTGYDIVVPGAVFAKPQIDGGLLMKLDKS
HHHHHHHHCCCEEEEEEECCCCCEEEEEEECCCCCEEEECCEEEECCCCCCCEEEEEEHH
KISNYGNLDSPIMEKLATIDPGNAYLVPWAWSFTTVGINKAKVAKALGSTPMPENAWELV
HHCCCCCCCHHHHHHHHCCCCCCEEEEEEEEEEEEECCCHHHHHHHHCCCCCCCCCHHHE
FNPVYTAKLKSCGIAFLDSPTEVIPPAMHYLGKNAYSNDPADHKAAAAMLAKVRPHIRMF
ECCHHHHHHHHCCEEEECCCHHHCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
SSTMIDDLAGGKACVALAWAGDINIARGRAIENKSGNDVQALLPSTGGLIFFDTLAIPKD
HHHHHHHHCCCCEEEEEEEECCCEEECCCEECCCCCCCEEEECCCCCCEEEEEEECCCCC
AKHPNNALAFINYSLRPEVSASLTNELGYATANKASLASVKPEIAQDKAVFPDAANLQKM
CCCCCCEEEEEEEECCCCHHHHHHHHHCCCCCCCCHHHHCCCHHHCCCCCCCCHHHHHHH
VSPASFSNEARESMSNVFTLFKKGS
HCCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIRKHLWIFAFSAVALSACGKKEEAPAVATPAAPASAAVAVNTEEKVLNVYNWPDYVAKD
CCCHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCEEEEECCHHHHEEECCCHHHHHHH
MVANFEKETGIKVNYQTFENNEALHAKLVAGNTGYDIVVPGAVFAKPQIDGGLLMKLDKS
HHHHHHHHCCCEEEEEEECCCCCEEEEEEECCCCCEEEECCEEEECCCCCCCEEEEEEHH
KISNYGNLDSPIMEKLATIDPGNAYLVPWAWSFTTVGINKAKVAKALGSTPMPENAWELV
HHCCCCCCCHHHHHHHHCCCCCCEEEEEEEEEEEEECCCHHHHHHHHCCCCCCCCCHHHE
FNPVYTAKLKSCGIAFLDSPTEVIPPAMHYLGKNAYSNDPADHKAAAAMLAKVRPHIRMF
ECCHHHHHHHHCCEEEECCCHHHCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
SSTMIDDLAGGKACVALAWAGDINIARGRAIENKSGNDVQALLPSTGGLIFFDTLAIPKD
HHHHHHHHCCCCEEEEEEEECCCEEECCCEECCCCCCCEEEECCCCCCEEEEEEECCCCC
AKHPNNALAFINYSLRPEVSASLTNELGYATANKASLASVKPEIAQDKAVFPDAANLQKM
CCCCCCEEEEEEEECCCCHHHHHHHHHCCCCCCCCHHHHCCCHHHCCCCCCCCHHHHHHH
VSPASFSNEARESMSNVFTLFKKGS
HCCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]