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The map label for this gene is potB [H]

Identifier: 91787897

GI number: 91787897

Start: 2091858

End: 2092715

Strand: Direct

Name: potB [H]

Synonym: Bpro_2021

Alternate gene names: 91787897

Gene position: 2091858-2092715 (Clockwise)

Preceding gene: 91787896

Following gene: 91787898

Centisome position: 40.23

GC content: 63.99

Gene sequence:

>858_bases
ATGTCCACTCTGAATTCCGGCCGCCTCCGGCTCGCCTTGCTGGCGCTGCCGGCCGTTGTACTCCTGCTGCCTTTTTTGCT
GGCCGTTGTCTTTGTGCTGCGCTTTTCCTTTTCGGCGGACACGCAGGCCATTGACGGTTTCAGCCTGGAGCGTTACCTGG
GCCTGCTCGACCCGTTTTTTCTTCGCTCGGTCGTCCTGACGCTGAAGCTGGCGGCGCTCAGCACGGTCATCTGTCTGGTG
CTGGCGGTGCCGGTGGCCATGTTGATGGCGGTGATTACCCAACCCATCTGGCGGCGCGTCGTCACCAGCCTGATCCTGCT
GCCCATGATCCTGAATCTGCTGATCCAGTCCTATGGTTGGATCATGGTGCTGGGGCCTAACGGCATCATCAATGGCGTGC
TGCTCAAGCTGGGCCTGCTGGACCGCCCGGTGCAGCTGCTGTTCAATGAAATCGGTGTGCTGGTGGGCCTGGTGCAAACC
GCCTTGCCGCTGGCCGTGTTTCCGATCCTCGGCTCGATGCGGGCCATCTCGGCGCAATACCTGGAGGCCAGTGCCAGTCT
GGGGGCCGCGCCATGGCGCTCGTTTCGCGACATCACGCTGCCGCTGCTCAAGCCCGGGCTGATCGGGGCGGCCTCGATTG
TGTTTGCCTTCAACGCCAGCGCTTTCGCCGTGCCGCTGCTGCTCGGCGGCCGCCGTGTCCAGACCATGGGCGTGGCCATC
CGCGACATGATTTCACCGATGTTTGACTGGGCAGGCGCGGCGGCGGCGGGCGTCACCCTCATTGCCATCACGCTGGCAAC
CCTGATGGCCGCCACCTGGCTGTCCACACGCACCACCCAATGGCAGGGAGCACGATGA

Upstream 100 bases:

>100_bases
CAGCTGGATCAAGCGTTATACGCAGACCTTCTCCTGATCGCCTGCAGTTGATAAACGACCGGGCCGCGTCCTGCGGTCCG
GTCGTCAGGAAAGTTTCACC

Downstream 100 bases:

>100_bases
TGGCAACGGCAACTTCAATGACCCCTTCAGTGACTTCTCCCGCTGCGTCCTCAACCACTTCCCGGGCCGGCCGCGGATCG
TTTGGCTGGGTCATGGGCAT

Product: binding-protein-dependent transport systems inner membrane component

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MSTLNSGRLRLALLALPAVVLLLPFLLAVVFVLRFSFSADTQAIDGFSLERYLGLLDPFFLRSVVLTLKLAALSTVICLV
LAVPVAMLMAVITQPIWRRVVTSLILLPMILNLLIQSYGWIMVLGPNGIINGVLLKLGLLDRPVQLLFNEIGVLVGLVQT
ALPLAVFPILGSMRAISAQYLEASASLGAAPWRSFRDITLPLLKPGLIGAASIVFAFNASAFAVPLLLGGRRVQTMGVAI
RDMISPMFDWAGAAAAGVTLIAITLATLMAATWLSTRTTQWQGAR

Sequences:

>Translated_285_residues
MSTLNSGRLRLALLALPAVVLLLPFLLAVVFVLRFSFSADTQAIDGFSLERYLGLLDPFFLRSVVLTLKLAALSTVICLV
LAVPVAMLMAVITQPIWRRVVTSLILLPMILNLLIQSYGWIMVLGPNGIINGVLLKLGLLDRPVQLLFNEIGVLVGLVQT
ALPLAVFPILGSMRAISAQYLEASASLGAAPWRSFRDITLPLLKPGLIGAASIVFAFNASAFAVPLLLGGRRVQTMGVAI
RDMISPMFDWAGAAAAGVTLIAITLATLMAATWLSTRTTQWQGAR
>Mature_284_residues
STLNSGRLRLALLALPAVVLLLPFLLAVVFVLRFSFSADTQAIDGFSLERYLGLLDPFFLRSVVLTLKLAALSTVICLVL
AVPVAMLMAVITQPIWRRVVTSLILLPMILNLLIQSYGWIMVLGPNGIINGVLLKLGLLDRPVQLLFNEIGVLVGLVQTA
LPLAVFPILGSMRAISAQYLEASASLGAAPWRSFRDITLPLLKPGLIGAASIVFAFNASAFAVPLLLGGRRVQTMGVAIR
DMISPMFDWAGAAAAGVTLIAITLATLMAATWLSTRTTQWQGAR

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=231, Percent_Identity=32.034632034632, Blast_Score=117, Evalue=6e-28,
Organism=Escherichia coli, GI1787080, Length=248, Percent_Identity=29.8387096774194, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1787713, Length=236, Percent_Identity=27.9661016949153, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30534; Mature: 30403

Theoretical pI: Translated: 11.54; Mature: 11.54

Prosite motif: PS50928 ABC_TM1 ; PS00237 G_PROTEIN_RECEP_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTLNSGRLRLALLALPAVVLLLPFLLAVVFVLRFSFSADTQAIDGFSLERYLGLLDPFF
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHCHHH
LRSVVLTLKLAALSTVICLVLAVPVAMLMAVITQPIWRRVVTSLILLPMILNLLIQSYGW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCE
IMVLGPNGIINGVLLKLGLLDRPVQLLFNEIGVLVGLVQTALPLAVFPILGSMRAISAQY
EEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEASASLGAAPWRSFRDITLPLLKPGLIGAASIVFAFNASAFAVPLLLGGRRVQTMGVAI
HHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHH
RDMISPMFDWAGAAAAGVTLIAITLATLMAATWLSTRTTQWQGAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
STLNSGRLRLALLALPAVVLLLPFLLAVVFVLRFSFSADTQAIDGFSLERYLGLLDPFF
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHCHHH
LRSVVLTLKLAALSTVICLVLAVPVAMLMAVITQPIWRRVVTSLILLPMILNLLIQSYGW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCE
IMVLGPNGIINGVLLKLGLLDRPVQLLFNEIGVLVGLVQTALPLAVFPILGSMRAISAQY
EEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEASASLGAAPWRSFRDITLPLLKPGLIGAASIVFAFNASAFAVPLLLGGRRVQTMGVAI
HHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHH
RDMISPMFDWAGAAAAGVTLIAITLATLMAATWLSTRTTQWQGAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]